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The map label for this gene is rpoA

Identifier: 116515234

GI number: 116515234

Start: 355988

End: 356977

Strand: Reverse

Name: rpoA

Synonym: BCc_316

Alternate gene names: 116515234

Gene position: 356977-355988 (Counterclockwise)

Preceding gene: 116515235

Following gene: 116515233

Centisome position: 85.73

GC content: 28.89

Gene sequence:

>990_bases
ATGCAAGGTTTTGTAGAAGATTTTTTAAAACCTAGATTAGTTGATATTCAACAAATTAGTGCCACACATGCTAAAATTAC
TTTAGAACCATTAGAACGTGGTTTTGGACATACTTTAGGAAATGCTTTACGAAGAATTTTATTATCTTCTATTTCAGGAT
GTGCAGTTACAGAGGTTGAAATAGATGGAATCTTACATGAATATATGCATAAGGAAGGAGTAAAAGAAGATATATTAGAT
ATTCTTTTAAATTTAAAAGGTTTATCAATAAAATTATTTGGAAAAGATGAAGTCACTTTATATTTAAAAAAAAGCGGGAT
TGGTCCAGTTTTAGCTAAAGATATTAATCATGATAATTCTACTGAAATTATAAATAACAAACACATTATTTGTAATCTTA
CTTGTTCGGATGCATCAATTGATATTAGAATGAAAGTAACACGAGGAAGAGGATATATTACAGCAAAATCTAGAAAAGAA
AATTATGATAATGAACATATTATTGGAAAACTCTTTCTTGATGTTACTTATAGCCCAATAGAACGTATTATGTATACAGT
TGAATCTGCCCGGGTAGAACAAAGAACTGATTTAGATAAATTAATTATAGAAATGGAAACTAATGGAACTATTGATCCTG
AAGATGCAATTAGAAAAGCAGCTACTATTTTAGCTCAACAACTAGAAGCTTTTGTAGATTTAAGAGGTGTTCGTGAGCCA
GAAATTAAAGAAGAAAAACCAGAATTTGAACCTGTTTTATTACGACCAGTAGATGATTTAGAATTAACTGTACGATCTGC
TAATTGTTTGAAAGCAGAAAGCATTCATTACATTGGTGATTTAGTACAAAAAACAGAAGTAGAATTACTCAAAACTCCAA
ATTTAGGTAAAAAATCACTTACTGAAATTAAAGATGTATTAGCATCACGTGGATTATCATTAGGAATGAAATTAGATAAT
TGGCCACCTAAAAGTCTTTTAGAAGATTAA

Upstream 100 bases:

>100_bases
TATAGAACGTGATGATTTATCTTCGGATATTAATGAGCATTTAATTATAGAATTATATTCGAAGTAACATTTTATATATT
CTATTAAGAGAGAAATAATA

Downstream 100 bases:

>100_bases
ATTTTTAAAATTTTAAAGGAAATAATTATGAGACATAGAAAAATTGGACGATTATTAAATAGAACACGAAGTCATTTAAA
ATCTATGTTTAAAAATATGA

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MQGFVEDFLKPRLVDIQQISATHAKITLEPLERGFGHTLGNALRRILLSSISGCAVTEVEIDGILHEYMHKEGVKEDILD
ILLNLKGLSIKLFGKDEVTLYLKKSGIGPVLAKDINHDNSTEIINNKHIICNLTCSDASIDIRMKVTRGRGYITAKSRKE
NYDNEHIIGKLFLDVTYSPIERIMYTVESARVEQRTDLDKLIIEMETNGTIDPEDAIRKAATILAQQLEAFVDLRGVREP
EIKEEKPEFEPVLLRPVDDLELTVRSANCLKAESIHYIGDLVQKTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMKLDN
WPPKSLLED

Sequences:

>Translated_329_residues
MQGFVEDFLKPRLVDIQQISATHAKITLEPLERGFGHTLGNALRRILLSSISGCAVTEVEIDGILHEYMHKEGVKEDILD
ILLNLKGLSIKLFGKDEVTLYLKKSGIGPVLAKDINHDNSTEIINNKHIICNLTCSDASIDIRMKVTRGRGYITAKSRKE
NYDNEHIIGKLFLDVTYSPIERIMYTVESARVEQRTDLDKLIIEMETNGTIDPEDAIRKAATILAQQLEAFVDLRGVREP
EIKEEKPEFEPVLLRPVDDLELTVRSANCLKAESIHYIGDLVQKTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMKLDN
WPPKSLLED
>Mature_329_residues
MQGFVEDFLKPRLVDIQQISATHAKITLEPLERGFGHTLGNALRRILLSSISGCAVTEVEIDGILHEYMHKEGVKEDILD
ILLNLKGLSIKLFGKDEVTLYLKKSGIGPVLAKDINHDNSTEIINNKHIICNLTCSDASIDIRMKVTRGRGYITAKSRKE
NYDNEHIIGKLFLDVTYSPIERIMYTVESARVEQRTDLDKLIIEMETNGTIDPEDAIRKAATILAQQLEAFVDLRGVREP
EIKEEKPEFEPVLLRPVDDLELTVRSANCLKAESIHYIGDLVQKTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMKLDN
WPPKSLLED

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=329, Percent_Identity=75.6838905775076, Blast_Score=525, Evalue=1e-150,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_BUCCC (Q057C9)

Other databases:

- EMBL:   CP000263
- RefSeq:   YP_802863.1
- ProteinModelPortal:   Q057C9
- STRING:   Q057C9
- EnsemblBacteria:   EBBUCT00000001356
- GeneID:   4440674
- GenomeReviews:   CP000263_GR
- KEGG:   bcc:BCc_316
- eggNOG:   COG0202
- GeneTree:   EBGT00050000008134
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- BioCyc:   BAPH372461:BCC_316-MONOMER
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 37009; Mature: 37009

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGFVEDFLKPRLVDIQQISATHAKITLEPLERGFGHTLGNALRRILLSSISGCAVTEVE
CCCHHHHHCCCCCCCHHHHCCCHHEEEHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEH
IDGILHEYMHKEGVKEDILDILLNLKGLSIKLFGKDEVTLYLKKSGIGPVLAKDINHDNS
HHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCCHHHHHCCCCCC
TEIINNKHIICNLTCSDASIDIRMKVTRGRGYITAKSRKENYDNEHIIGKLFLDVTYSPI
CEEECCCEEEEEEEECCCEEEEEEEEECCCEEEEECCCCCCCCCCCEEEEEEEECCHHHH
ERIMYTVESARVEQRTDLDKLIIEMETNGTIDPEDAIRKAATILAQQLEAFVDLRGVREP
HHHHHHHHHHHHHHHCCHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EIKEEKPEFEPVLLRPVDDLELTVRSANCLKAESIHYIGDLVQKTEVELLKTPNLGKKSL
CCCCCCCCCCCEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
TEIKDVLASRGLSLGMKLDNWPPKSLLED
HHHHHHHHHCCCEECCCCCCCCCHHHCCC
>Mature Secondary Structure
MQGFVEDFLKPRLVDIQQISATHAKITLEPLERGFGHTLGNALRRILLSSISGCAVTEVE
CCCHHHHHCCCCCCCHHHHCCCHHEEEHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEH
IDGILHEYMHKEGVKEDILDILLNLKGLSIKLFGKDEVTLYLKKSGIGPVLAKDINHDNS
HHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCCHHHHHCCCCCC
TEIINNKHIICNLTCSDASIDIRMKVTRGRGYITAKSRKENYDNEHIIGKLFLDVTYSPI
CEEECCCEEEEEEEECCCEEEEEEEEECCCEEEEECCCCCCCCCCCEEEEEEEECCHHHH
ERIMYTVESARVEQRTDLDKLIIEMETNGTIDPEDAIRKAATILAQQLEAFVDLRGVREP
HHHHHHHHHHHHHHHCCHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EIKEEKPEFEPVLLRPVDDLELTVRSANCLKAESIHYIGDLVQKTEVELLKTPNLGKKSL
CCCCCCCCCCCEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
TEIKDVLASRGLSLGMKLDNWPPKSLLED
HHHHHHHHHCCCEECCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA