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The map label for this gene is pnp

Identifier: 116515160

GI number: 116515160

Start: 262614

End: 264695

Strand: Reverse

Name: pnp

Synonym: BCc_227

Alternate gene names: 116515160

Gene position: 264695-262614 (Counterclockwise)

Preceding gene: 116515161

Following gene: 116515159

Centisome position: 63.57

GC content: 26.51

Gene sequence:

>2082_bases
TTGTTAAAACCAATTATACATAAATTTAAGTATGGAAAACATAATATTATTTTAGAAACCGGAGTAATAGCTCGACAAGC
TACTGCTTCAGTTTTAGCAAGTATGGATGATACTACAGTATTAATAACTATTGTTAGTGATAATACTACTATTCCTGGGC
AAAAATTTTTTCCTTTAGTAGTAAATTATCAAGAACGAACTTATGCTGCTGGTAGAATACCCGGTGGATTTTTTCGTCGT
GAAGGACGACCTAGTGAAAATGAAATTTTAATTTCTCGATTGATTGATAGACCAATTAGACCTTTATTTCCTAAAGATTT
TTTATATGAAGTGCAAATTATTGCAACAGTTATTTCTGTAAATCCACAAATTAATCCTGATATTATTTCTATAATTGGTT
CATCTGCAGCATTATGTTTATCAGGATTACCTTTCTTAGGTCCTATTGGTGCAGCTCGTATTGGTTTTTTAAATAATAAA
TATATTTTAAATCCTTCTATAGAAAAAATAAAAAAAAGTAGTTTAGATTTAGTAGTTTCTGGAACTAAAAATACTATTTT
TATGGTAGAAGCTGAAGCTAGCATATTGTCTGAAGATAATATTTTAAATGCTATATTATTTGGTCATGAATCTCAACAAT
CGTTAATTGATAATATTTATTTGTTTTCTGATAAAGCAAATAAAATACCAAATATATATAAAAATATTTATAAACCTGAT
AAACAGATATTTAATTTAATTTCTAAAAAAATAAAAAAAGACATTAATAAAGCATATTATATTTTAAAGAAAAAAGAGAG
ATTAAAAAAATTAAATGATATTAAACAAAAAGTTACTTCAGAATTATTAAACAACGATAGTCAATTAACTAGTTCAAAAA
TTGAAGAAAGTATTTATTTTTTAGAACGTAATATTGTAAGAAAACGTATTTTAAAAGGAAAATTAAGAATAGATGGTCGT
ATCAACAATGAGGTTAGAAAAATAGATGTAAGAACAGGTATATTACCAAGAGTTCACGGATCTGCTCTTTTTACTCGAGG
AGAAACACAAGCTTTAGTATCAGCAACCTTAGGGACATCTCGAGATGCACAAAATTTAGATGATTTATTAGGAGACAGAA
CAGATAATTTTTTATTTCATTATAATTTTCCACCTTATTCAGTAGGAGAAATAGGAATAGTAGGATCTCCAAAAAGAAGA
GAAATAGGACATGGTAAGTTAGCTAAACGCAGTTTTTTAGCTGTTATGCCAAATATTGAAGATTTTCCATATACAATTCG
TTTAGTATCTGAAATAACTGAATCTAATGGATCTTCTTCTATGGCTTCTGTTTGTGGTGCATCTTTAGCTTTAATGGATG
CTGGAGTTCCTATTAAATCCGCAATTGCTGGAATTGCAATGGGTTTAATTAAAGAAAATGATTCTTATATAATTCTTTCT
GATATATTAGGTGATGAAGATTATTTAGGTGATATGGATTTTAAAGTATCAGGAAGTAGATTAGGTATTACTGCTTTACA
AATGGATATTAAAGTTTCTGGAATTACTAGTGATATTATTAGATCTGCTTTATATCAAGCAAAATCAGCTAGATTAAAAA
TATTAGATATTATGGAAAATACTTTACAAATTCCTAGAAGTGATATTTCAAAATTTGCTCCTAGAATTTATACTATAAAA
ATAAATCCAGAAAAAATAAAAGATGTTATTGGAAAAGGAGGTTCAATTATTAGAATGCTGACAGAAGAAACTGGTACTGT
TATTGAAATTAAAGATGATGGAATAGTAAAAATTTCTGCAATTAATGGTGAAAAAGCTAAATATGCAATTAAAAGAATAC
AAGAAATCACTGAAGATATACAAATAGGAAAAATTTATTCAGGAAAAGTTACAAGAATTTTAGATTTTGGAGCTTTTGTT
TCTATCGGATTTGGTAAAGAAGGATTAATTCATATTTCTCAAATTTCTAATAAAAGAATTGATAAAGTGATAAATCATTT
AAAAATTAATCAAATTATTTTTGTAAAAGTTTTAGAGGTTGATCGACAAGGTCGAATAAGATTAAGTATGAAGAATATTT
AA

Upstream 100 bases:

>100_bases
ATTTAGGTTATTTTTTATTTAAATAAATAAATTTATTATTAAAAATTTTTTAATAGATATAAATATATTTTCAAATTATT
TTATTTTAAGGATATTAATT

Downstream 100 bases:

>100_bases
AATTTATATTTTTTAAAAAAAATTTGTTTATATTTTGAATAAAATATATGTACTATCTTATTAGATAATTTTATTGTATA
ATAAATTCTTAATGAATTTA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 693; Mature: 693

Protein sequence:

>693_residues
MLKPIIHKFKYGKHNIILETGVIARQATASVLASMDDTTVLITIVSDNTTIPGQKFFPLVVNYQERTYAAGRIPGGFFRR
EGRPSENEILISRLIDRPIRPLFPKDFLYEVQIIATVISVNPQINPDIISIIGSSAALCLSGLPFLGPIGAARIGFLNNK
YILNPSIEKIKKSSLDLVVSGTKNTIFMVEAEASILSEDNILNAILFGHESQQSLIDNIYLFSDKANKIPNIYKNIYKPD
KQIFNLISKKIKKDINKAYYILKKKERLKKLNDIKQKVTSELLNNDSQLTSSKIEESIYFLERNIVRKRILKGKLRIDGR
INNEVRKIDVRTGILPRVHGSALFTRGETQALVSATLGTSRDAQNLDDLLGDRTDNFLFHYNFPPYSVGEIGIVGSPKRR
EIGHGKLAKRSFLAVMPNIEDFPYTIRLVSEITESNGSSSMASVCGASLALMDAGVPIKSAIAGIAMGLIKENDSYIILS
DILGDEDYLGDMDFKVSGSRLGITALQMDIKVSGITSDIIRSALYQAKSARLKILDIMENTLQIPRSDISKFAPRIYTIK
INPEKIKDVIGKGGSIIRMLTEETGTVIEIKDDGIVKISAINGEKAKYAIKRIQEITEDIQIGKIYSGKVTRILDFGAFV
SIGFGKEGLIHISQISNKRIDKVINHLKINQIIFVKVLEVDRQGRIRLSMKNI

Sequences:

>Translated_693_residues
MLKPIIHKFKYGKHNIILETGVIARQATASVLASMDDTTVLITIVSDNTTIPGQKFFPLVVNYQERTYAAGRIPGGFFRR
EGRPSENEILISRLIDRPIRPLFPKDFLYEVQIIATVISVNPQINPDIISIIGSSAALCLSGLPFLGPIGAARIGFLNNK
YILNPSIEKIKKSSLDLVVSGTKNTIFMVEAEASILSEDNILNAILFGHESQQSLIDNIYLFSDKANKIPNIYKNIYKPD
KQIFNLISKKIKKDINKAYYILKKKERLKKLNDIKQKVTSELLNNDSQLTSSKIEESIYFLERNIVRKRILKGKLRIDGR
INNEVRKIDVRTGILPRVHGSALFTRGETQALVSATLGTSRDAQNLDDLLGDRTDNFLFHYNFPPYSVGEIGIVGSPKRR
EIGHGKLAKRSFLAVMPNIEDFPYTIRLVSEITESNGSSSMASVCGASLALMDAGVPIKSAIAGIAMGLIKENDSYIILS
DILGDEDYLGDMDFKVSGSRLGITALQMDIKVSGITSDIIRSALYQAKSARLKILDIMENTLQIPRSDISKFAPRIYTIK
INPEKIKDVIGKGGSIIRMLTEETGTVIEIKDDGIVKISAINGEKAKYAIKRIQEITEDIQIGKIYSGKVTRILDFGAFV
SIGFGKEGLIHISQISNKRIDKVINHLKINQIIFVKVLEVDRQGRIRLSMKNI
>Mature_693_residues
MLKPIIHKFKYGKHNIILETGVIARQATASVLASMDDTTVLITIVSDNTTIPGQKFFPLVVNYQERTYAAGRIPGGFFRR
EGRPSENEILISRLIDRPIRPLFPKDFLYEVQIIATVISVNPQINPDIISIIGSSAALCLSGLPFLGPIGAARIGFLNNK
YILNPSIEKIKKSSLDLVVSGTKNTIFMVEAEASILSEDNILNAILFGHESQQSLIDNIYLFSDKANKIPNIYKNIYKPD
KQIFNLISKKIKKDINKAYYILKKKERLKKLNDIKQKVTSELLNNDSQLTSSKIEESIYFLERNIVRKRILKGKLRIDGR
INNEVRKIDVRTGILPRVHGSALFTRGETQALVSATLGTSRDAQNLDDLLGDRTDNFLFHYNFPPYSVGEIGIVGSPKRR
EIGHGKLAKRSFLAVMPNIEDFPYTIRLVSEITESNGSSSMASVCGASLALMDAGVPIKSAIAGIAMGLIKENDSYIILS
DILGDEDYLGDMDFKVSGSRLGITALQMDIKVSGITSDIIRSALYQAKSARLKILDIMENTLQIPRSDISKFAPRIYTIK
INPEKIKDVIGKGGSIIRMLTEETGTVIEIKDDGIVKISAINGEKAKYAIKRIQEITEDIQIGKIYSGKVTRILDFGAFV
SIGFGKEGLIHISQISNKRIDKVINHLKINQIIFVKVLEVDRQGRIRLSMKNI

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=702, Percent_Identity=36.7521367521368, Blast_Score=429, Evalue=1e-120,
Organism=Escherichia coli, GI145693187, Length=693, Percent_Identity=64.9350649350649, Blast_Score=966, Evalue=0.0,
Organism=Escherichia coli, GI87082262, Length=159, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI115534063, Length=717, Percent_Identity=31.9386331938633, Blast_Score=338, Evalue=4e-93,
Organism=Drosophila melanogaster, GI281362905, Length=708, Percent_Identity=37.1468926553672, Blast_Score=449, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24651641, Length=708, Percent_Identity=37.1468926553672, Blast_Score=449, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24651643, Length=708, Percent_Identity=37.1468926553672, Blast_Score=449, Evalue=1e-126,
Organism=Drosophila melanogaster, GI161079377, Length=655, Percent_Identity=36.9465648854962, Blast_Score=410, Evalue=1e-114,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_BUCCC (Q057K3)

Other databases:

- EMBL:   CP000263
- RefSeq:   YP_802789.1
- ProteinModelPortal:   Q057K3
- STRING:   Q057K3
- EnsemblBacteria:   EBBUCT00000001446
- GeneID:   4440802
- GenomeReviews:   CP000263_GR
- KEGG:   bcc:BCc_227
- eggNOG:   COG1185
- GeneTree:   EBGT00050000007805
- HOGENOM:   HBG382411
- OMA:   KRVDNVD
- ProtClustDB:   PRK11824
- BioCyc:   BAPH372461:BCC_227-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 77155; Mature: 77155

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKPIIHKFKYGKHNIILETGVIARQATASVLASMDDTTVLITIVSDNTTIPGQKFFPLV
CCCHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCEEE
VNYQERTYAAGRIPGGFFRREGRPSENEILISRLIDRPIRPLFPKDFLYEVQIIATVISV
EEECHHEEECCCCCCHHHHCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCC
NPQINPDIISIIGSSAALCLSGLPFLGPIGAARIGFLNNKYILNPSIEKIKKSSLDLVVS
CCCCCCHHHHHHCCCHHHHHCCCCCCCCCCHHHEEEECCEEEECCCHHHHHCCCCCEEEE
GTKNTIFMVEAEASILSEDNILNAILFGHESQQSLIDNIYLFSDKANKIPNIYKNIYKPD
CCCCEEEEEECCHHHCCCCCCEEEEEECCCHHHHHHHEEEEECCCCCCCCHHHHHHCCCH
KQIFNLISKKIKKDINKAYYILKKKERLKKLNDIKQKVTSELLNNDSQLTSSKIEESIYF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LERNIVRKRILKGKLRIDGRINNEVRKIDVRTGILPRVHGSALFTRGETQALVSATLGTS
HHHHHHHHHHHHCCEEECCEECCCEEEEEECCCCCCCCCCCEEEECCCHHHHHHHHCCCC
RDAQNLDDLLGDRTDNFLFHYNFPPYSVGEIGIVGSPKRREIGHGKLAKRSFLAVMPNIE
CCHHHHHHHHCCCCCCEEEEECCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHEECCCCC
DFPYTIRLVSEITESNGSSSMASVCGASLALMDAGVPIKSAIAGIAMGLIKENDSYIILS
CCCHHHHHHHHHHHCCCCHHHHHHHCCHHEEHCCCCCHHHHHHHHHHHHEECCCCEEEEE
DILGDEDYLGDMDFKVSGSRLGITALQMDIKVSGITSDIIRSALYQAKSARLKILDIMEN
ECCCCCCCCCCCCEEECCCEEEEEEEEEEEEEECCHHHHHHHHHHHHHCCCEEHHHHHHH
TLQIPRSDISKFAPRIYTIKINPEKIKDVIGKGGSIIRMLTEETGTVIEIKDDGIVKISA
HHCCCHHHHHHHCCEEEEEEECHHHHHHHHCCCCCEEEEEECCCCCEEEECCCCEEEEEE
INGEKAKYAIKRIQEITEDIQIGKIYSGKVTRILDFGAFVSIGFGKEGLIHISQISNKRI
CCCCHHHHHHHHHHHHHHHCEECEEECCCEEEEEECCCEEEECCCCCCCEEEEHHCCHHH
DKVINHLKINQIIFVKVLEVDRQGRIRLSMKNI
HHHHHHHCCCCEEEEEEEEECCCCCEEEEECCC
>Mature Secondary Structure
MLKPIIHKFKYGKHNIILETGVIARQATASVLASMDDTTVLITIVSDNTTIPGQKFFPLV
CCCHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCEEE
VNYQERTYAAGRIPGGFFRREGRPSENEILISRLIDRPIRPLFPKDFLYEVQIIATVISV
EEECHHEEECCCCCCHHHHCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCC
NPQINPDIISIIGSSAALCLSGLPFLGPIGAARIGFLNNKYILNPSIEKIKKSSLDLVVS
CCCCCCHHHHHHCCCHHHHHCCCCCCCCCCHHHEEEECCEEEECCCHHHHHCCCCCEEEE
GTKNTIFMVEAEASILSEDNILNAILFGHESQQSLIDNIYLFSDKANKIPNIYKNIYKPD
CCCCEEEEEECCHHHCCCCCCEEEEEECCCHHHHHHHEEEEECCCCCCCCHHHHHHCCCH
KQIFNLISKKIKKDINKAYYILKKKERLKKLNDIKQKVTSELLNNDSQLTSSKIEESIYF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LERNIVRKRILKGKLRIDGRINNEVRKIDVRTGILPRVHGSALFTRGETQALVSATLGTS
HHHHHHHHHHHHCCEEECCEECCCEEEEEECCCCCCCCCCCEEEECCCHHHHHHHHCCCC
RDAQNLDDLLGDRTDNFLFHYNFPPYSVGEIGIVGSPKRREIGHGKLAKRSFLAVMPNIE
CCHHHHHHHHCCCCCCEEEEECCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHEECCCCC
DFPYTIRLVSEITESNGSSSMASVCGASLALMDAGVPIKSAIAGIAMGLIKENDSYIILS
CCCHHHHHHHHHHHCCCCHHHHHHHCCHHEEHCCCCCHHHHHHHHHHHHEECCCCEEEEE
DILGDEDYLGDMDFKVSGSRLGITALQMDIKVSGITSDIIRSALYQAKSARLKILDIMEN
ECCCCCCCCCCCCEEECCCEEEEEEEEEEEEEECCHHHHHHHHHHHHHCCCEEHHHHHHH
TLQIPRSDISKFAPRIYTIKINPEKIKDVIGKGGSIIRMLTEETGTVIEIKDDGIVKISA
HHCCCHHHHHHHCCEEEEEEECHHHHHHHHCCCCCEEEEEECCCCCEEEECCCCEEEEEE
INGEKAKYAIKRIQEITEDIQIGKIYSGKVTRILDFGAFVSIGFGKEGLIHISQISNKRI
CCCCHHHHHHHHHHHHHHHCEECEEECCCEEEEEECCCEEEECCCCCCCEEEEHHCCHHH
DKVINHLKINQIIFVKVLEVDRQGRIRLSMKNI
HHHHHHHCCCCEEEEEEEEECCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA