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The map label for this gene is pykA [H]

Identifier: 116515134

GI number: 116515134

Start: 232481

End: 233920

Strand: Reverse

Name: pykA [H]

Synonym: BCc_198

Alternate gene names: 116515134

Gene position: 233920-232481 (Counterclockwise)

Preceding gene: 116515139

Following gene: 116515129

Centisome position: 56.18

GC content: 24.24

Gene sequence:

>1440_bases
ATGATACATTTTTTTAGAAGAACAAAAATTATTTCTACATTGGGACCATCTACAGATAAAAAAAGTGTATTAAAAAAAAT
AATACAATCCGGAGCAAATGCATTAAGATTAAATTTTTCTCATGGTACTTCTATAGATCACATAAATAGAGTAAAAAATA
TTCGAGAAATTGAAAAAGAATTAAATTGTTTTGTTTCTTTAATTGGTGATTTACAAGGACCGAAAATTAGAATTTCTAGT
TTTAAAAAAAAAAAAATTTTTTTAAATAAAGGAGATAATTTTTTATTAGATTTAAGTATTCCAAAATATTATGGTAATAA
AAATTCTGTAGGTATTAATTATAAAAATTTACCTAATGAAGTTAATTCTGGCGACATTCTCTTATTAGATGACGGAAAAA
TACAATTACGAGTTTTAAAAAAAAATAATAGAAAAATTTTTACTAAAGTAATTATTGGAGGATATTTATCTGATAATAAA
GGTTTAAATAAATTAGGAGGAGGACTATCAGCATCTGCATTAACAAAAAAAGATGAAAAAGACATAAAATTAGCCGCAAT
GTTAGGAGTAGATTATATAGCTGTGTCTTTTCCTAGATCTAAAGAAGATTTACAAAAAGCACGAAAATTAATAAAAAAAT
CAGGTAGTAAAGCTAAAATTATTGCAAAAATTGAACGAGCAGAAGCTGTATCATCAAACAATGTAATGAAAGATATAATT
TTAGAATCTGATGTTATTATGATTGCTAGAGGAGATTTAGGAGTAGAAATCGGTGATCATGAGTTAATTGGAATACAAAA
AAAATTAATTTCAATGACTAGAAAATTAAATCGAATAGTAATTACTGCTACACAAATGATGGAATCAATGATAGAAAATC
CTTTTCCTACTAGAGCAGAAGTTATGGATATTGCAAATTCTGTATTAGATGGTACAGATGCGGTTATGTTATCAGCAGAA
ACTGCATCAGGCAGTTTTCCTGAAATAACAGTAAAAACTATGTCAAAAATTTGTTTAGGAGCTGAAAAAGTTCCTAGTGC
AAAAATTTCTAAACATCGTTTAAATGAAAAATTTAGTGATGTTGATGAAACTATTGCTATGTCTGCTATGTATTCTGCTA
ATCATTTAAAAAATGTTTCTGCAATAATAATTTTTTTAACATCAAGAAAAATTGCTTTGTTAGCATCAAGAATTACATCA
GGATTACCTATTTTTTATATATCAAATTGTGTTAAAAAACTTCGTCTATCAACATTATATCGAGGTGTAATTCCTATTTA
TTTTAATAAAAATAATTTTAATAATCATGATAAAGTTATAATAAAGTTTCTAAAAAAGAAAAAATTTTTAAAAAAAAATG
AAATTATTGTCATTATAAAAAATGATTTAGAAAGAAACAGTAAAATTACAAATACTTGTAAAATTATCAATGTAACATAA

Upstream 100 bases:

>100_bases
TTATAATAAATAAAATATACATATATTTTTATATAAAATTGATTATTTTTTATATATATTTATTTAAAATATTTCTCTTA
TTTTTAAAGGAAATTTAAGT

Downstream 100 bases:

>100_bases
TTATTTTATTAATTTAATTAAATTAAAATTTCTGTTTTGATTATATATGAATAAATATATTTTATTTTTGTATAAAAATA
TTTTTATTAATATGCTAATT

Product: PykA

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MIHFFRRTKIISTLGPSTDKKSVLKKIIQSGANALRLNFSHGTSIDHINRVKNIREIEKELNCFVSLIGDLQGPKIRISS
FKKKKIFLNKGDNFLLDLSIPKYYGNKNSVGINYKNLPNEVNSGDILLLDDGKIQLRVLKKNNRKIFTKVIIGGYLSDNK
GLNKLGGGLSASALTKKDEKDIKLAAMLGVDYIAVSFPRSKEDLQKARKLIKKSGSKAKIIAKIERAEAVSSNNVMKDII
LESDVIMIARGDLGVEIGDHELIGIQKKLISMTRKLNRIVITATQMMESMIENPFPTRAEVMDIANSVLDGTDAVMLSAE
TASGSFPEITVKTMSKICLGAEKVPSAKISKHRLNEKFSDVDETIAMSAMYSANHLKNVSAIIIFLTSRKIALLASRITS
GLPIFYISNCVKKLRLSTLYRGVIPIYFNKNNFNNHDKVIIKFLKKKKFLKKNEIIVIIKNDLERNSKITNTCKIINVT

Sequences:

>Translated_479_residues
MIHFFRRTKIISTLGPSTDKKSVLKKIIQSGANALRLNFSHGTSIDHINRVKNIREIEKELNCFVSLIGDLQGPKIRISS
FKKKKIFLNKGDNFLLDLSIPKYYGNKNSVGINYKNLPNEVNSGDILLLDDGKIQLRVLKKNNRKIFTKVIIGGYLSDNK
GLNKLGGGLSASALTKKDEKDIKLAAMLGVDYIAVSFPRSKEDLQKARKLIKKSGSKAKIIAKIERAEAVSSNNVMKDII
LESDVIMIARGDLGVEIGDHELIGIQKKLISMTRKLNRIVITATQMMESMIENPFPTRAEVMDIANSVLDGTDAVMLSAE
TASGSFPEITVKTMSKICLGAEKVPSAKISKHRLNEKFSDVDETIAMSAMYSANHLKNVSAIIIFLTSRKIALLASRITS
GLPIFYISNCVKKLRLSTLYRGVIPIYFNKNNFNNHDKVIIKFLKKKKFLKKNEIIVIIKNDLERNSKITNTCKIINVT
>Mature_479_residues
MIHFFRRTKIISTLGPSTDKKSVLKKIIQSGANALRLNFSHGTSIDHINRVKNIREIEKELNCFVSLIGDLQGPKIRISS
FKKKKIFLNKGDNFLLDLSIPKYYGNKNSVGINYKNLPNEVNSGDILLLDDGKIQLRVLKKNNRKIFTKVIIGGYLSDNK
GLNKLGGGLSASALTKKDEKDIKLAAMLGVDYIAVSFPRSKEDLQKARKLIKKSGSKAKIIAKIERAEAVSSNNVMKDII
LESDVIMIARGDLGVEIGDHELIGIQKKLISMTRKLNRIVITATQMMESMIENPFPTRAEVMDIANSVLDGTDAVMLSAE
TASGSFPEITVKTMSKICLGAEKVPSAKISKHRLNEKFSDVDETIAMSAMYSANHLKNVSAIIIFLTSRKIALLASRITS
GLPIFYISNCVKKLRLSTLYRGVIPIYFNKNNFNNHDKVIIKFLKKKKFLKKNEIIVIIKNDLERNSKITNTCKIINVT

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=500, Percent_Identity=34.2, Blast_Score=238, Evalue=9e-63,
Organism=Homo sapiens, GI33286422, Length=499, Percent_Identity=33.2665330661323, Blast_Score=238, Evalue=1e-62,
Organism=Homo sapiens, GI33286420, Length=499, Percent_Identity=33.2665330661323, Blast_Score=238, Evalue=1e-62,
Organism=Homo sapiens, GI32967597, Length=500, Percent_Identity=32.6, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI10835121, Length=500, Percent_Identity=32.6, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI310128732, Length=291, Percent_Identity=35.3951890034364, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI310128730, Length=291, Percent_Identity=35.3951890034364, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=35.0210970464135, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=35.0210970464135, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI310128738, Length=216, Percent_Identity=34.2592592592593, Blast_Score=102, Evalue=9e-22,
Organism=Escherichia coli, GI1788160, Length=483, Percent_Identity=57.7639751552795, Blast_Score=582, Evalue=1e-167,
Organism=Escherichia coli, GI1787965, Length=425, Percent_Identity=35.0588235294118, Blast_Score=248, Evalue=7e-67,
Organism=Caenorhabditis elegans, GI17544584, Length=491, Percent_Identity=34.4195519348269, Blast_Score=251, Evalue=4e-67,
Organism=Caenorhabditis elegans, GI17506829, Length=497, Percent_Identity=35.2112676056338, Blast_Score=238, Evalue=4e-63,
Organism=Caenorhabditis elegans, GI17506831, Length=497, Percent_Identity=35.2112676056338, Blast_Score=238, Evalue=5e-63,
Organism=Caenorhabditis elegans, GI71984413, Length=497, Percent_Identity=35.2112676056338, Blast_Score=238, Evalue=6e-63,
Organism=Caenorhabditis elegans, GI71984406, Length=497, Percent_Identity=35.2112676056338, Blast_Score=238, Evalue=6e-63,
Organism=Saccharomyces cerevisiae, GI6319279, Length=471, Percent_Identity=33.7579617834395, Blast_Score=230, Evalue=4e-61,
Organism=Saccharomyces cerevisiae, GI6324923, Length=454, Percent_Identity=31.7180616740088, Blast_Score=211, Evalue=2e-55,
Organism=Drosophila melanogaster, GI28571814, Length=497, Percent_Identity=32.7967806841046, Blast_Score=237, Evalue=1e-62,
Organism=Drosophila melanogaster, GI24648964, Length=497, Percent_Identity=32.7967806841046, Blast_Score=237, Evalue=1e-62,
Organism=Drosophila melanogaster, GI24581235, Length=434, Percent_Identity=32.7188940092166, Blast_Score=216, Evalue=3e-56,
Organism=Drosophila melanogaster, GI24648966, Length=422, Percent_Identity=33.175355450237, Blast_Score=209, Evalue=3e-54,
Organism=Drosophila melanogaster, GI24646914, Length=276, Percent_Identity=34.7826086956522, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 53457; Mature: 53457

Theoretical pI: Translated: 10.64; Mature: 10.64

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHFFRRTKIISTLGPSTDKKSVLKKIIQSGANALRLNFSHGTSIDHINRVKNIREIEKE
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
LNCFVSLIGDLQGPKIRISSFKKKKIFLNKGDNFLLDLSIPKYYGNKNSVGINYKNLPNE
HHHHHHHHHCCCCCEEEECCCCCEEEEEECCCCEEEEEECHHHCCCCCCCCEEECCCCCC
VNSGDILLLDDGKIQLRVLKKNNRKIFTKVIIGGYLSDNKGLNKLGGGLSASALTKKDEK
CCCCCEEEEECCCEEEEEEECCCCEEEEEEEEECEECCCCCHHHHCCCCCHHHHCCCCCC
DIKLAAMLGVDYIAVSFPRSKEDLQKARKLIKKSGSKAKIIAKIERAEAVSSNNVMKDII
CEEEEEHHCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHCCCCHHHHHH
LESDVIMIARGDLGVEIGDHELIGIQKKLISMTRKLNRIVITATQMMESMIENPFPTRAE
CCCCEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHCCCCCCHHH
VMDIANSVLDGTDAVMLSAETASGSFPEITVKTMSKICLGAEKVPSAKISKHRLNEKFSD
HHHHHHHHCCCCCEEEEEEECCCCCCCEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
VDETIAMSAMYSANHLKNVSAIIIFLTSRKIALLASRITSGLPIFYISNCVKKLRLSTLY
HHHHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RGVIPIYFNKNNFNNHDKVIIKFLKKKKFLKKNEIIVIIKNDLERNSKITNTCKIINVT
HCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEEEEC
>Mature Secondary Structure
MIHFFRRTKIISTLGPSTDKKSVLKKIIQSGANALRLNFSHGTSIDHINRVKNIREIEKE
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
LNCFVSLIGDLQGPKIRISSFKKKKIFLNKGDNFLLDLSIPKYYGNKNSVGINYKNLPNE
HHHHHHHHHCCCCCEEEECCCCCEEEEEECCCCEEEEEECHHHCCCCCCCCEEECCCCCC
VNSGDILLLDDGKIQLRVLKKNNRKIFTKVIIGGYLSDNKGLNKLGGGLSASALTKKDEK
CCCCCEEEEECCCEEEEEEECCCCEEEEEEEEECEECCCCCHHHHCCCCCHHHHCCCCCC
DIKLAAMLGVDYIAVSFPRSKEDLQKARKLIKKSGSKAKIIAKIERAEAVSSNNVMKDII
CEEEEEHHCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHCCCCHHHHHH
LESDVIMIARGDLGVEIGDHELIGIQKKLISMTRKLNRIVITATQMMESMIENPFPTRAE
CCCCEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHCCCCCCHHH
VMDIANSVLDGTDAVMLSAETASGSFPEITVKTMSKICLGAEKVPSAKISKHRLNEKFSD
HHHHHHHHCCCCCEEEEEEECCCCCCCEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
VDETIAMSAMYSANHLKNVSAIIIFLTSRKIALLASRITSGLPIFYISNCVKKLRLSTLY
HHHHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RGVIPIYFNKNNFNNHDKVIIKFLKKKKFLKKNEIIVIIKNDLERNSKITNTCKIINVT
HCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]