Click here to switch to the map view.

The map label for this gene is aspS [H]

Identifier: 116515132

GI number: 116515132

Start: 229678

End: 231393

Strand: Direct

Name: aspS [H]

Synonym: BCc_196

Alternate gene names: 116515132

Gene position: 229678-231393 (Clockwise)

Preceding gene: 116515131

Following gene: 116515133

Centisome position: 55.16

GC content: 18.82

Gene sequence:

>1716_bases
ATGCGAACTAAATATTGTGGAGAAATAAAAAAAAAAGATATTAACAAAATAATAACTATATGTGGTTGGGTACATAAAAT
AAGAAGTCTTAAAAATATAATTTTTATTGATATTAAAGATATAACTGGAATAATACAAGTTATTTTTCTTAAAAAATATA
ATTCTAATTTTTCTTTAGTAAAAACAATAAGAAATGATTATTGTATAAAAATTAAAGGATTAGTTATTTTAAAAAAAAAA
TTAACTTATTTTAATACACAAGAAAAAGAAAATTTTTTTGAAATTATTGCATTTAAATTAAAAATATTTAATCCCTCACT
ACCTATACCTTTTGATTATAAAAAAGAAAATAAAGAAGATATTAGATTTAAATTTCGTTATTTAGATTTACGTCGATTTT
ATATGTTTCATAAATTACAAACAAGAAGTAAAATTACAACAATTATTAGAAATTTTTTTATAAAAAAAAAATTTTTAGAA
ATTGAAACACCTATTTTAACACGTTCTACTCCTGAAGGAGCAAAAGACTATTTAGTATATAGTAGATTATATCCTGGTAA
ATATTTTGCATTACCACAATCACCTCAATTATTTAAACAATTATTAATGATATCTGGAATAGATCGTTATTTTCAAATTG
CTAAATGTTTTCGTGATGAGGATTTAAGATCAGATAGACAGCCAGAATTTACACAAATTGATATTGAAATAGCTTTTAAA
AAAAAGAAATTTTTACAAAAATTAATTAATAAATTAATAAAAAAATTATGGTTAAAAATTAATAATTATACTATTAAAAA
AATTAAAAATATTACTTATAAAGAAAGTATTTTAAAATACGGAACAGATAAACCAGATTTAAGAAATCCTATACAATTTG
TTGAGTTATATGATATTTTTAATAATTTTTATAAAAAATTTTTTCCATGGTTATTAATAAAAAATATTAATAGAATAATT
TCTATAAAAATACCTGAAGGAATTAAAAAAATACGTGATAATAAAATTTATAAATATAAAAAATTTTTAGAAAAACTAAA
TTTTAATTATTTTATATATGTGAAAATTATAAACATTAAAAAATATATTATTAAAGATAATCGAAACATATTTAGTAAAA
TAGATAAAAAAACATTAAAAGAATTTTTTTTAAAAACTTCAGCTATTAATGGAGATATTATATTTATAATTGCAGAAAAA
TATAATATTGCAAACAATATTAGTAATCTTTTACGAAAGAAAATTGGTATAGATTTAAAAATCACAAATTTAAAAAAAAT
ATGTCCAGTTTGGATTACAAAATTTCCTTTATTTAAATTAGATAAAAAAAATAATTTAAAATCTGTTCATCATCCTTTTA
CTTCACCTGAAAAAAATAGTAAAAATATTGAATTATTAAATCCATTAAAAATTATTTCTAGTGCATTTGATTTAGTAATT
AATGGTTATGAAATTGGTGGAGGTTCTGAAAGAATTTATAATTATCAATTACAAATAAAAATTTTTAATATATTAAAAAT
TAACAAAAATAAACAAAAAAAAAAATTTGGTTTCTTCATAAATTCTTTACAATATGGAGCCCCTCCTCATTTAGGATTAG
CGCTGGGATTAGATAGATTAGTTATGTTATTAACAAACTGTAATACAATCAGTGATGTAATTGCATTTCCTAAAACTAAT
TCTGCAATTTGTTTAATGACTGGAGCGCCTTGTTGA

Upstream 100 bases:

>100_bases
GATTTGAGTAAAGGTCGAATAATTTTTCGTAGTAGATAGTATTTAAAATATTTTTAATATTAAATTAATAAATTTAAAAT
TAATTGAAATTAGGATTTTT

Downstream 100 bases:

>100_bases
TTATTTAATTAAAAATCAAAATTATAAAAATTAAATTAATAATTCTAAATATGTTTAATATAATCATTACTTTTAAAAAT
ATTAAAATATCTAAATGAGG

Product: AspS

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MRTKYCGEIKKKDINKIITICGWVHKIRSLKNIIFIDIKDITGIIQVIFLKKYNSNFSLVKTIRNDYCIKIKGLVILKKK
LTYFNTQEKENFFEIIAFKLKIFNPSLPIPFDYKKENKEDIRFKFRYLDLRRFYMFHKLQTRSKITTIIRNFFIKKKFLE
IETPILTRSTPEGAKDYLVYSRLYPGKYFALPQSPQLFKQLLMISGIDRYFQIAKCFRDEDLRSDRQPEFTQIDIEIAFK
KKKFLQKLINKLIKKLWLKINNYTIKKIKNITYKESILKYGTDKPDLRNPIQFVELYDIFNNFYKKFFPWLLIKNINRII
SIKIPEGIKKIRDNKIYKYKKFLEKLNFNYFIYVKIINIKKYIIKDNRNIFSKIDKKTLKEFFLKTSAINGDIIFIIAEK
YNIANNISNLLRKKIGIDLKITNLKKICPVWITKFPLFKLDKKNNLKSVHHPFTSPEKNSKNIELLNPLKIISSAFDLVI
NGYEIGGGSERIYNYQLQIKIFNILKINKNKQKKKFGFFINSLQYGAPPHLGLALGLDRLVMLLTNCNTISDVIAFPKTN
SAICLMTGAPC

Sequences:

>Translated_571_residues
MRTKYCGEIKKKDINKIITICGWVHKIRSLKNIIFIDIKDITGIIQVIFLKKYNSNFSLVKTIRNDYCIKIKGLVILKKK
LTYFNTQEKENFFEIIAFKLKIFNPSLPIPFDYKKENKEDIRFKFRYLDLRRFYMFHKLQTRSKITTIIRNFFIKKKFLE
IETPILTRSTPEGAKDYLVYSRLYPGKYFALPQSPQLFKQLLMISGIDRYFQIAKCFRDEDLRSDRQPEFTQIDIEIAFK
KKKFLQKLINKLIKKLWLKINNYTIKKIKNITYKESILKYGTDKPDLRNPIQFVELYDIFNNFYKKFFPWLLIKNINRII
SIKIPEGIKKIRDNKIYKYKKFLEKLNFNYFIYVKIINIKKYIIKDNRNIFSKIDKKTLKEFFLKTSAINGDIIFIIAEK
YNIANNISNLLRKKIGIDLKITNLKKICPVWITKFPLFKLDKKNNLKSVHHPFTSPEKNSKNIELLNPLKIISSAFDLVI
NGYEIGGGSERIYNYQLQIKIFNILKINKNKQKKKFGFFINSLQYGAPPHLGLALGLDRLVMLLTNCNTISDVIAFPKTN
SAICLMTGAPC
>Mature_571_residues
MRTKYCGEIKKKDINKIITICGWVHKIRSLKNIIFIDIKDITGIIQVIFLKKYNSNFSLVKTIRNDYCIKIKGLVILKKK
LTYFNTQEKENFFEIIAFKLKIFNPSLPIPFDYKKENKEDIRFKFRYLDLRRFYMFHKLQTRSKITTIIRNFFIKKKFLE
IETPILTRSTPEGAKDYLVYSRLYPGKYFALPQSPQLFKQLLMISGIDRYFQIAKCFRDEDLRSDRQPEFTQIDIEIAFK
KKKFLQKLINKLIKKLWLKINNYTIKKIKNITYKESILKYGTDKPDLRNPIQFVELYDIFNNFYKKFFPWLLIKNINRII
SIKIPEGIKKIRDNKIYKYKKFLEKLNFNYFIYVKIINIKKYIIKDNRNIFSKIDKKTLKEFFLKTSAINGDIIFIIAEK
YNIANNISNLLRKKIGIDLKITNLKKICPVWITKFPLFKLDKKNNLKSVHHPFTSPEKNSKNIELLNPLKIISSAFDLVI
NGYEIGGGSERIYNYQLQIKIFNILKINKNKQKKKFGFFINSLQYGAPPHLGLALGLDRLVMLLTNCNTISDVIAFPKTN
SAICLMTGAPC

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=587, Percent_Identity=35.4344122657581, Blast_Score=343, Evalue=3e-94,
Organism=Homo sapiens, GI5031815, Length=321, Percent_Identity=25.5451713395639, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI194272210, Length=321, Percent_Identity=25.5451713395639, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI45439306, Length=248, Percent_Identity=25.4032258064516, Blast_Score=73, Evalue=6e-13,
Organism=Escherichia coli, GI1788173, Length=572, Percent_Identity=42.6573426573427, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI1790571, Length=283, Percent_Identity=26.1484098939929, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789256, Length=297, Percent_Identity=26.5993265993266, Blast_Score=86, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI32566633, Length=597, Percent_Identity=32.1608040201005, Blast_Score=270, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI71994340, Length=301, Percent_Identity=25.5813953488372, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17535925, Length=301, Percent_Identity=25.5813953488372, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17535927, Length=301, Percent_Identity=25.5813953488372, Blast_Score=85, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6325153, Length=627, Percent_Identity=32.5358851674641, Blast_Score=253, Evalue=4e-68,
Organism=Saccharomyces cerevisiae, GI6320242, Length=251, Percent_Identity=26.2948207171315, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323011, Length=123, Percent_Identity=31.7073170731707, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24584738, Length=618, Percent_Identity=33.495145631068, Blast_Score=291, Evalue=8e-79,
Organism=Drosophila melanogaster, GI24640849, Length=265, Percent_Identity=26.0377358490566, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24640851, Length=265, Percent_Identity=26.0377358490566, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 67692; Mature: 67692

Theoretical pI: Translated: 10.60; Mature: 10.60

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTKYCGEIKKKDINKIITICGWVHKIRSLKNIIFIDIKDITGIIQVIFLKKYNSNFSLV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHCCCCHHHH
KTIRNDYCIKIKGLVILKKKLTYFNTQEKENFFEIIAFKLKIFNPSLPIPFDYKKENKED
HHHCCCEEEEEEEEEEEEEHHHHCCCCCHHHHHHHHEEEEEEECCCCCCCCCCCCCCCCC
IRFKFRYLDLRRFYMFHKLQTRSKITTIIRNFFIKKKFLEIETPILTRSTPEGAKDYLVY
EEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEECCCCCCCHHHEEE
SRLYPGKYFALPQSPQLFKQLLMISGIDRYFQIAKCFRDEDLRSDRQPEFTQIDIEIAFK
EEECCCEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHCCHHHCCCCCCCEEEEEEEEEEH
KKKFLQKLINKLIKKLWLKINNYTIKKIKNITYKESILKYGTDKPDLRNPIQFVELYDIF
HHHHHHHHHHHHHHHHHHHHCCEEEEHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
NNFYKKFFPWLLIKNINRIISIKIPEGIKKIRDNKIYKYKKFLEKLNFNYFIYVKIINIK
HHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEEEEEE
KYIIKDNRNIFSKIDKKTLKEFFLKTSAINGDIIFIIAEKYNIANNISNLLRKKIGIDLK
EEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCEE
ITNLKKICPVWITKFPLFKLDKKNNLKSVHHPFTSPEKNSKNIELLNPLKIISSAFDLVI
ECCHHHHHHHHHHCCCCEEECCCCCCCHHCCCCCCCCCCCCCEEEECHHHHHHHHHHHEE
NGYEIGGGSERIYNYQLQIKIFNILKINKNKQKKKFGFFINSLQYGAPPHLGLALGLDRL
ECEEECCCCCEEEEEEEEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHH
VMLLTNCNTISDVIAFPKTNSAICLMTGAPC
HHHHHCCCHHHHHHCCCCCCCEEEEEECCCC
>Mature Secondary Structure
MRTKYCGEIKKKDINKIITICGWVHKIRSLKNIIFIDIKDITGIIQVIFLKKYNSNFSLV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHCCCCHHHH
KTIRNDYCIKIKGLVILKKKLTYFNTQEKENFFEIIAFKLKIFNPSLPIPFDYKKENKED
HHHCCCEEEEEEEEEEEEEHHHHCCCCCHHHHHHHHEEEEEEECCCCCCCCCCCCCCCCC
IRFKFRYLDLRRFYMFHKLQTRSKITTIIRNFFIKKKFLEIETPILTRSTPEGAKDYLVY
EEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEECCCCCCCHHHEEE
SRLYPGKYFALPQSPQLFKQLLMISGIDRYFQIAKCFRDEDLRSDRQPEFTQIDIEIAFK
EEECCCEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHCCHHHCCCCCCCEEEEEEEEEEH
KKKFLQKLINKLIKKLWLKINNYTIKKIKNITYKESILKYGTDKPDLRNPIQFVELYDIF
HHHHHHHHHHHHHHHHHHHHCCEEEEHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
NNFYKKFFPWLLIKNINRIISIKIPEGIKKIRDNKIYKYKKFLEKLNFNYFIYVKIINIK
HHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEEEEEE
KYIIKDNRNIFSKIDKKTLKEFFLKTSAINGDIIFIIAEKYNIANNISNLLRKKIGIDLK
EEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCEE
ITNLKKICPVWITKFPLFKLDKKNNLKSVHHPFTSPEKNSKNIELLNPLKIISSAFDLVI
ECCHHHHHHHHHHCCCCEEECCCCCCCHHCCCCCCCCCCCCCEEEECHHHHHHHHHHHEE
NGYEIGGGSERIYNYQLQIKIFNILKINKNKQKKKFGFFINSLQYGAPPHLGLALGLDRL
ECEEECCCCCEEEEEEEEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHH
VMLLTNCNTISDVIAFPKTNSAICLMTGAPC
HHHHHCCCHHHHHHCCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA