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The map label for this gene is sucB [H]

Identifier: 116515121

GI number: 116515121

Start: 217701

End: 218897

Strand: Direct

Name: sucB [H]

Synonym: BCc_185

Alternate gene names: 116515121

Gene position: 217701-218897 (Clockwise)

Preceding gene: 116515120

Following gene: 116515122

Centisome position: 52.28

GC content: 20.13

Gene sequence:

>1197_bases
ATGGAAAAAAAAACTAAAATATTAGCTCCCAATTTACCTGAATCAGTAAATCATGCAATTATGTTAAAATGGAATAAAAA
AATAGGAGATTATGTAAAAGAAGATGAAATAATTGCTGAAATTGAAACTGATAAAATTATACTTGAAATTTCATCTCCTA
AAAATGGTATATTAATTTCACAGAATATATTAGTTGGAGAAAAAATAAAATCTCAATCTGTTATAGGTTTTATTAATAAT
AAAAATATTAAAAAAGAAAAAAAAATTAAAAATAATAAAAAAACAAAAAAAAAAAATGTTCATAGTGAAAATTCATTATT
TTTGTTTACACCAAAAATGCGTAGGTTAATACTAAATTATAATATAGATATATCAAAAATAAAAGGAATTGATGTACATG
GAAAAATTAATATAAATAATTTTATTATTAAAAATAAAAAAAATAGCAAAAAGAAAAAAAAAGATAAACTAATTAATATA
CATAAAAAAAATAATAAACGAATTTTTCGAAAAATTGCTATGAGTCCGTTACGAAAAACGATTTCAAAAAGATTATTATA
TACAGTAAAAAATACAGCTATGTTAACAACATTTAATGAAGTTAATATGCAGCCAATTATATCAATTAGAAACAAATATA
AAGATATTTTTGAAAATAAATATAAATCAAAATTGGGATTTATGTCGTTTTATGTAAAATCTGTTACACAAGCATTAAAA
AAATTTCCAGAAATAAATGCTTCAATTGAAAAAAAAAATATTATATATCATGATTATTATGATATTAATATTGCTATATC
TACTCCAAGAGGATTAATAACTCCAATTTTAAAAAATACAGATAATTTATCTATATATGAAATTGAAAAAAAAATTAAAT
CTTTTGTTTTATTAGGTGAACAAGGAAAATTAAAATTTGAAGATTTAGAAGCTGGAACTTTTACTATTACAAATGGAGGA
GTTTTTGGATCTTTAATGTCTACTCCTATTATTAATCCTCCTCAAGTAGCTATTTTAGGTATGCATCATATTAAAAAAAG
ACCTATTGTAGTTAATAAAAAAATAAAAATTTTACCTATGATGTATCTTGCATTGTCATATGATCACCAATTAATTGATG
GAAAACAAGCAATACAATTTTTGAATTATATTAAAGATATTTTAGAAGATATTTCACGTTTTATACTTGAAATATAA

Upstream 100 bases:

>100_bases
CTTCTACAGCTGAAGGAAACTTTGAAAATCATCAAAAAAAACAAAAAAAAATTATTAAATTATCTTTTACATATAAATCA
TTTTATAAAAAGTAAAAATT

Downstream 100 bases:

>100_bases
ATTTTTATATAAAAATTATATATTAATTGAAAAAAATTTTTACTAAATATTAAAATATGTATAATATATTTTTATTGCAT
ATTAAAATATACAATAATAA

Product: 2-oxoglutarate dehydrogenase E2 component

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MEKKTKILAPNLPESVNHAIMLKWNKKIGDYVKEDEIIAEIETDKIILEISSPKNGILISQNILVGEKIKSQSVIGFINN
KNIKKEKKIKNNKKTKKKNVHSENSLFLFTPKMRRLILNYNIDISKIKGIDVHGKININNFIIKNKKNSKKKKKDKLINI
HKKNNKRIFRKIAMSPLRKTISKRLLYTVKNTAMLTTFNEVNMQPIISIRNKYKDIFENKYKSKLGFMSFYVKSVTQALK
KFPEINASIEKKNIIYHDYYDINIAISTPRGLITPILKNTDNLSIYEIEKKIKSFVLLGEQGKLKFEDLEAGTFTITNGG
VFGSLMSTPIINPPQVAILGMHHIKKRPIVVNKKIKILPMMYLALSYDHQLIDGKQAIQFLNYIKDILEDISRFILEI

Sequences:

>Translated_398_residues
MEKKTKILAPNLPESVNHAIMLKWNKKIGDYVKEDEIIAEIETDKIILEISSPKNGILISQNILVGEKIKSQSVIGFINN
KNIKKEKKIKNNKKTKKKNVHSENSLFLFTPKMRRLILNYNIDISKIKGIDVHGKININNFIIKNKKNSKKKKKDKLINI
HKKNNKRIFRKIAMSPLRKTISKRLLYTVKNTAMLTTFNEVNMQPIISIRNKYKDIFENKYKSKLGFMSFYVKSVTQALK
KFPEINASIEKKNIIYHDYYDINIAISTPRGLITPILKNTDNLSIYEIEKKIKSFVLLGEQGKLKFEDLEAGTFTITNGG
VFGSLMSTPIINPPQVAILGMHHIKKRPIVVNKKIKILPMMYLALSYDHQLIDGKQAIQFLNYIKDILEDISRFILEI
>Mature_398_residues
MEKKTKILAPNLPESVNHAIMLKWNKKIGDYVKEDEIIAEIETDKIILEISSPKNGILISQNILVGEKIKSQSVIGFINN
KNIKKEKKIKNNKKTKKKNVHSENSLFLFTPKMRRLILNYNIDISKIKGIDVHGKININNFIIKNKKNSKKKKKDKLINI
HKKNNKRIFRKIAMSPLRKTISKRLLYTVKNTAMLTTFNEVNMQPIISIRNKYKDIFENKYKSKLGFMSFYVKSVTQALK
KFPEINASIEKKNIIYHDYYDINIAISTPRGLITPILKNTDNLSIYEIEKKIKSFVLLGEQGKLKFEDLEAGTFTITNGG
VFGSLMSTPIINPPQVAILGMHHIKKRPIVVNKKIKILPMMYLALSYDHQLIDGKQAIQFLNYIKDILEDISRFILEI

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=230, Percent_Identity=46.9565217391304, Blast_Score=231, Evalue=1e-60,
Organism=Homo sapiens, GI31711992, Length=424, Percent_Identity=25, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI110671329, Length=420, Percent_Identity=27.1428571428571, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI203098753, Length=450, Percent_Identity=23.7777777777778, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI203098816, Length=450, Percent_Identity=23.7777777777778, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=31.055900621118, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI1786946, Length=402, Percent_Identity=46.0199004975124, Blast_Score=390, Evalue=1e-110,
Organism=Escherichia coli, GI1786305, Length=404, Percent_Identity=23.5148514851485, Blast_Score=128, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI25146366, Length=401, Percent_Identity=33.6658354114713, Blast_Score=246, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI17537937, Length=415, Percent_Identity=23.855421686747, Blast_Score=141, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17560088, Length=440, Percent_Identity=24.3181818181818, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17538894, Length=258, Percent_Identity=32.5581395348837, Blast_Score=121, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6320352, Length=404, Percent_Identity=35.6435643564356, Blast_Score=270, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6324258, Length=447, Percent_Identity=22.5950782997763, Blast_Score=112, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24645909, Length=221, Percent_Identity=46.606334841629, Blast_Score=226, Evalue=3e-59,
Organism=Drosophila melanogaster, GI18859875, Length=425, Percent_Identity=24, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI20129315, Length=232, Percent_Identity=30.1724137931034, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24582497, Length=232, Percent_Identity=30.1724137931034, Blast_Score=116, Evalue=3e-26,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 45984; Mature: 45984

Theoretical pI: Translated: 10.67; Mature: 10.67

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKTKILAPNLPESVNHAIMLKWNKKIGDYVKEDEIIAEIETDKIILEISSPKNGILIS
CCCCCCEECCCCCCCCCCEEEEEECCHHHCHHCCCCEEEEEECCEEEEEECCCCCCEEEE
QNILVGEKIKSQSVIGFINNKNIKKEKKIKNNKKTKKKNVHSENSLFLFTPKMRRLILNY
CCEEECCCCCCCCEEEEECCCCCCHHHHHHCCCHHHHHCCCCCCCEEEECHHHHEEEEEC
NIDISKIKGIDVHGKININNFIIKNKKNSKKKKKDKLINIHKKNNKRIFRKIAMSPLRKT
CCCHHHEECEEEEEEEEECEEEEECCCCCHHHHHHHEEEEEECCCHHHHHHHHHHHHHHH
ISKRLLYTVKNTAMLTTFNEVNMQPIISIRNKYKDIFENKYKSKLGFMSFYVKSVTQALK
HHHHHEEEEECCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFPEINASIEKKNIIYHDYYDINIAISTPRGLITPILKNTDNLSIYEIEKKIKSFVLLGE
HCCCCCCCEECCEEEEEEEEEEEEEEECCCCHHHHHHCCCCCCEEEHHHHHHHHEEEECC
QGKLKFEDLEAGTFTITNGGVFGSLMSTPIINPPQVAILGMHHIKKRPIVVNKKIKILPM
CCCEEEEECCCCEEEEECCCEEHHHHHCCCCCCCCEEEEEHHHHCCCCEEECCCCHHHHH
MYLALSYDHQLIDGKQAIQFLNYIKDILEDISRFILEI
HHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEKKTKILAPNLPESVNHAIMLKWNKKIGDYVKEDEIIAEIETDKIILEISSPKNGILIS
CCCCCCEECCCCCCCCCCEEEEEECCHHHCHHCCCCEEEEEECCEEEEEECCCCCCEEEE
QNILVGEKIKSQSVIGFINNKNIKKEKKIKNNKKTKKKNVHSENSLFLFTPKMRRLILNY
CCEEECCCCCCCCEEEEECCCCCCHHHHHHCCCHHHHHCCCCCCCEEEECHHHHEEEEEC
NIDISKIKGIDVHGKININNFIIKNKKNSKKKKKDKLINIHKKNNKRIFRKIAMSPLRKT
CCCHHHEECEEEEEEEEECEEEEECCCCCHHHHHHHEEEEEECCCHHHHHHHHHHHHHHH
ISKRLLYTVKNTAMLTTFNEVNMQPIISIRNKYKDIFENKYKSKLGFMSFYVKSVTQALK
HHHHHEEEEECCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFPEINASIEKKNIIYHDYYDINIAISTPRGLITPILKNTDNLSIYEIEKKIKSFVLLGE
HCCCCCCCEECCEEEEEEEEEEEEEEECCCCHHHHHHCCCCCCEEEHHHHHHHHEEEECC
QGKLKFEDLEAGTFTITNGGVFGSLMSTPIINPPQVAILGMHHIKKRPIVVNKKIKILPM
CCCEEEEECCCCEEEEECCCEEHHHHHCCCCCCCCEEEEEHHHHCCCCEEECCCCHHHHH
MYLALSYDHQLIDGKQAIQFLNYIKDILEDISRFILEI
HHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]