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The map label for this gene is proS [H]

Identifier: 116515089

GI number: 116515089

Start: 176442

End: 177710

Strand: Reverse

Name: proS [H]

Synonym: BCc_149

Alternate gene names: 116515089

Gene position: 177710-176442 (Counterclockwise)

Preceding gene: 116515092

Following gene: 116515081

Centisome position: 42.68

GC content: 20.17

Gene sequence:

>1269_bases
ATGTATACAAAAAAATATTTATTATCAACTTTAAAAGAAAAACCAAAAAATATAGAATCTATTAGTCATCAACTTATGCT
TCGATCAGGAATGATTAGAATGTTATCATCTGGTATATACACCTGGCTTCCTACTGGTTATCGTATAATTAAGAAAATAA
CAAAGATTATTTCTAGTATATTAAATAATCATGGATGCATGGAAATTTGTATGCCTATCATACAACCGGAAAATTTATGG
AAAAAAAGCGGAAGAAAAAAATTATATGGAGAAGAATTAATTAAAATAACTGATCGAAGAAACAAAAAATATGTATTAAG
TCCTACGCATGAAGAAGCTATAAATTATATTTGTCAACAAGAAATACATTCCTATAAACAATTACCAATTACTTTTTATC
AAATACAAAAAAAATTTAGAGATGAAATTCGACCAAGATTTGGTGTAATACGAACAATAGAATTTCTCATGAAAGATGCA
TATTCATTTCATATTAACAAAGATTCATTAAAAAAAACATATGAAAATATGAAAAATATATATATAAAAATATTTAAAAA
ATTAAAATTAAATTTTAAAATAGTTAAAGCTCATTCAAAAATTATGGGAGGATCAATATCAGATGAATTTCATGCTATTT
CTGAAATGGGTGAAAACAAAATTATATTTTTGAAAAAATCTAATTATGCTTTTAATTATAATGAATTTAATGAAAAAAAA
AAAAAATTTAAAAATTATTTACTACAAAAAGAAAAAAAACTAATAAAAAAAAACAGTATAGAAATTGGACATATTTTCCA
AATTGAAAAAAAATATTCTAAAAATACAAATTTTTATATTCAGGATAAAAAAAACAAAAAAAAATTACTTAACATGGGTT
GTTATGGAATCGGAATAAATAGAATAATATCTTCAATTATAGAACAAAATCATGATAAATACGGAATTATTTGGCCTAAT
TGTATAGCACCTTTTCAAGTATTTATACTTCCAATTAATATGTATAAAAACAAAAAAATAAAAAAAATAGCAAAAAAACT
TTATAAAAAATTAATAAATAAAAATATTGATGTTTTATTTGATAACAGAAATGAAAGTTTAGGAAAAATGTTTGCAGATA
TGAATTTAATTGGAATACCATTTGGTATTTTAATTAGTAAAAATACAATTACATCTAAAAAAGTAGAATTATATTCAAGA
AAAAAAAACTCTAAAAAAAAAGTATTATACTCCAAAATAATATCAATTATAAAAAATAAAATAATATAA

Upstream 100 bases:

>100_bases
TTAATAAATAAAAGTATTTTATTAAAAAAAAAAATTAATTAATTTATGTTGAAAATATCTTAATTATTTTATAAATTTAT
TAATATTCAGGATAAAAATA

Downstream 100 bases:

>100_bases
TTCACTAAATAAAAAATCTATTTAAAAAAATTTAATTTAATAATTATTTGTTTTTTAAAAAATTTTAAATAATTTAAAAA
ATCATTATCTGGAAAAATTT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MYTKKYLLSTLKEKPKNIESISHQLMLRSGMIRMLSSGIYTWLPTGYRIIKKITKIISSILNNHGCMEICMPIIQPENLW
KKSGRKKLYGEELIKITDRRNKKYVLSPTHEEAINYICQQEIHSYKQLPITFYQIQKKFRDEIRPRFGVIRTIEFLMKDA
YSFHINKDSLKKTYENMKNIYIKIFKKLKLNFKIVKAHSKIMGGSISDEFHAISEMGENKIIFLKKSNYAFNYNEFNEKK
KKFKNYLLQKEKKLIKKNSIEIGHIFQIEKKYSKNTNFYIQDKKNKKKLLNMGCYGIGINRIISSIIEQNHDKYGIIWPN
CIAPFQVFILPINMYKNKKIKKIAKKLYKKLINKNIDVLFDNRNESLGKMFADMNLIGIPFGILISKNTITSKKVELYSR
KKNSKKKVLYSKIISIIKNKII

Sequences:

>Translated_422_residues
MYTKKYLLSTLKEKPKNIESISHQLMLRSGMIRMLSSGIYTWLPTGYRIIKKITKIISSILNNHGCMEICMPIIQPENLW
KKSGRKKLYGEELIKITDRRNKKYVLSPTHEEAINYICQQEIHSYKQLPITFYQIQKKFRDEIRPRFGVIRTIEFLMKDA
YSFHINKDSLKKTYENMKNIYIKIFKKLKLNFKIVKAHSKIMGGSISDEFHAISEMGENKIIFLKKSNYAFNYNEFNEKK
KKFKNYLLQKEKKLIKKNSIEIGHIFQIEKKYSKNTNFYIQDKKNKKKLLNMGCYGIGINRIISSIIEQNHDKYGIIWPN
CIAPFQVFILPINMYKNKKIKKIAKKLYKKLINKNIDVLFDNRNESLGKMFADMNLIGIPFGILISKNTITSKKVELYSR
KKNSKKKVLYSKIISIIKNKII
>Mature_422_residues
MYTKKYLLSTLKEKPKNIESISHQLMLRSGMIRMLSSGIYTWLPTGYRIIKKITKIISSILNNHGCMEICMPIIQPENLW
KKSGRKKLYGEELIKITDRRNKKYVLSPTHEEAINYICQQEIHSYKQLPITFYQIQKKFRDEIRPRFGVIRTIEFLMKDA
YSFHINKDSLKKTYENMKNIYIKIFKKLKLNFKIVKAHSKIMGGSISDEFHAISEMGENKIIFLKKSNYAFNYNEFNEKK
KKFKNYLLQKEKKLIKKNSIEIGHIFQIEKKYSKNTNFYIQDKKNKKKLLNMGCYGIGINRIISSIIEQNHDKYGIIWPN
CIAPFQVFILPINMYKNKKIKKIAKKLYKKLINKNIDVLFDNRNESLGKMFADMNLIGIPFGILISKNTITSKKVELYSR
KKNSKKKVLYSKIISIIKNKII

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro) [H]

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=388, Percent_Identity=33.2474226804124, Blast_Score=246, Evalue=2e-65,
Organism=Escherichia coli, GI1786392, Length=232, Percent_Identity=54.3103448275862, Blast_Score=298, Evalue=4e-82,
Organism=Escherichia coli, GI1788013, Length=391, Percent_Identity=19.9488491048593, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI115532348, Length=399, Percent_Identity=32.8320802005013, Blast_Score=213, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI193203271, Length=125, Percent_Identity=30.4, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320931, Length=205, Percent_Identity=38.5365853658537, Blast_Score=156, Evalue=7e-39,
Organism=Drosophila melanogaster, GI24656200, Length=395, Percent_Identity=31.8987341772152, Blast_Score=226, Evalue=2e-59,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 49940; Mature: 49940

Theoretical pI: Translated: 10.64; Mature: 10.64

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTKKYLLSTLKEKPKNIESISHQLMLRSGMIRMLSSGIYTWLPTGYRIIKKITKIISSI
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHH
LNNHGCMEICMPIIQPENLWKKSGRKKLYGEELIKITDRRNKKYVLSPTHEEAINYICQQ
HCCCCHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHH
EIHSYKQLPITFYQIQKKFRDEIRPRFGVIRTIEFLMKDAYSFHINKDSLKKTYENMKNI
HHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHH
YIKIFKKLKLNFKIVKAHSKIMGGSISDEFHAISEMGENKIIFLKKSNYAFNYNEFNEKK
HHHHHHHHHCCEEEEEHHHHHHCCCCCHHHHHHHHCCCCEEEEEEECCCEECHHHHHHHH
KKFKNYLLQKEKKLIKKNSIEIGHIFQIEKKYSKNTNFYIQDKKNKKKLLNMGCYGIGIN
HHHHHHHHHHHHHHHHHCCCCEEHEEEEHHHHCCCCCEEEEECCCHHHHHHCCCHHCCHH
RIISSIIEQNHDKYGIIWPNCIAPFQVFILPINMYKNKKIKKIAKKLYKKLINKNIDVLF
HHHHHHHHCCCCCEEEECCCHHCCCEEEEEEEHHHCCCHHHHHHHHHHHHHHCCCCEEEE
DNRNESLGKMFADMNLIGIPFGILISKNTITSKKVELYSRKKNSKKKVLYSKIISIIKNK
CCCCHHHHHHHHCCCEEECCEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
II
CC
>Mature Secondary Structure
MYTKKYLLSTLKEKPKNIESISHQLMLRSGMIRMLSSGIYTWLPTGYRIIKKITKIISSI
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHH
LNNHGCMEICMPIIQPENLWKKSGRKKLYGEELIKITDRRNKKYVLSPTHEEAINYICQQ
HCCCCHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHH
EIHSYKQLPITFYQIQKKFRDEIRPRFGVIRTIEFLMKDAYSFHINKDSLKKTYENMKNI
HHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHH
YIKIFKKLKLNFKIVKAHSKIMGGSISDEFHAISEMGENKIIFLKKSNYAFNYNEFNEKK
HHHHHHHHHCCEEEEEHHHHHHCCCCCHHHHHHHHCCCCEEEEEEECCCEECHHHHHHHH
KKFKNYLLQKEKKLIKKNSIEIGHIFQIEKKYSKNTNFYIQDKKNKKKLLNMGCYGIGIN
HHHHHHHHHHHHHHHHHCCCCEEHEEEEHHHHCCCCCEEEEECCCHHHHHHCCCHHCCHH
RIISSIIEQNHDKYGIIWPNCIAPFQVFILPINMYKNKKIKKIAKKLYKKLINKNIDVLF
HHHHHHHHCCCCCEEEECCCHHCCCEEEEEEEHHHCCCHHHHHHHHHHHHHHCCCCEEEE
DNRNESLGKMFADMNLIGIPFGILISKNTITSKKVELYSRKKNSKKKVLYSKIISIIKNK
CCCCHHHHHHHHCCCEEECCEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
II
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA