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The map label for this gene is ilvI [H]

Identifier: 116515079

GI number: 116515079

Start: 163331

End: 165055

Strand: Reverse

Name: ilvI [H]

Synonym: BCc_139

Alternate gene names: 116515079

Gene position: 165055-163331 (Counterclockwise)

Preceding gene: 116515080

Following gene: 116515078

Centisome position: 39.64

GC content: 26.55

Gene sequence:

>1725_bases
ATGACATTATTATCTGGTGCCGAAATGGTTGTTCAATCATTAATGAACTTAAAAATAAAAAAAATATTTGGTTATCCAGG
AGGGGCTGTTTTAGATATTTATGACGCAATTCATACATTAGGTAAAAAAAAAATACAGCATATTTTAGTACGTCATGAAC
AAGCAGCTGTACATATGGCAGATGGATACGCACGATGTACTGGAAAAATCGGAGTTGTTTTAGTTACTTCAGGTCCTGGA
GCTACAAATGCAATTACTGGAATTGCTACTGCATACATGGATTCTATACCATTATTAATAATATCAGGACAAGTATCTTC
AAAATTAATTGGTTTTGATGCATTTCAAGAATGTGACATGATAGGTATTTCTCGACCTATTGTAAAACATAGTTTTTTAG
TAAAAAAATCTGAGAATATTCCTTTAATTTTTCAAAAATCGTATTTTTTAGCAACTAACGGAAGGCCTGGACCAATAGTA
ATTGATTTACCAAAAAATATTTTAGATCATAAAATAAAAAAAAAATATATTTGGGAAAATACAATTAATATTAAATCTTA
TAAAAACAACATAAAAAAAAATAAAAAAAATATAAAAAATGCATTAAATTTTTTATTAAAATCTAAAAAACCAATTATAT
ATGCTGGAGGAGGAATTATAAGCTCTAATAGTCACTCAGAATTATATAATTTAGCAACAAAATTAAATATACCAGTAACA
ATGTCTCTTATGTCATTAGGTTCTTTTCCTAGTAATCATCCACAAAATTTATCTATGTTAGGTATGCACGGAACTTATGA
AGCTAATATGGCTATGCATCATTCTGATATAATTCTAGCTATTGGAGTTAGATTTGATGATAGAACTACAAATAATGTAG
ATAAATATTGTCCAAATGCAAAAATAATACATATTGATATTGATCCTACATCAATTTCTAAAACAATAACAGCAGATATT
CCTATTATTGGAGATGCTAAAAAAATACTAAAAAAAATGTTATTATTATTAGAAAAAAAAGAAAAAAAAAGATCAAACTC
TTATTTAAATAATTGGTGGAAAAAAATACAAAAATGGAAAAATTATAAATTTAATAAATATAAAAATTCTAATAAAATAA
TTAAACCACAATATATCCTTCAAATACTACATAAATTAACAGAAGGAAAAGCATATATTACATCAGACGTAGGGCAACAT
CAAATGTTTGCTGCATTATATTATGGTTTTAATAAACCCCGTCATTGGATTAACTCAGGAGGGTTAGGTACAATGGGATT
TGGATTACCGGCTGCTCTAGGAGTAAAAATTGCACATCCTAAAAAATTTGTAATATGTGTTACTGGAGATGGTAGTATAC
AAATGAACATACAAGAATTATCAACAGCAAAACAATATAATTTAGCAATATTAATTATTAATTTAAATAATCAATCACTA
GGAATGGTAAAACAATGGCAAGACATGATTTATTCAGGTAGACACTCTCATTCATACATGCAATCATTACCTAACTTTAT
AAAACTAGCTAAAGCATATGGACATATTGGAATAAAAATAAAAAAACCAAAAGAAATAATCTCTAAATTAATATTAGCGA
TTAAACAGGTTCAATCTGGATCACTAGTTTTTGTTGATATTAATATTGATCCATATGAACATGTTTATCCTATGCAAATA
AAAAACGGTGGTATGAACGAAATGTTATTAAAAAAAACAGGATAA

Upstream 100 bases:

>100_bases
AAATTTTTTATTTATAATTAAATTATAAAAAATATATTAATTATAAATATATATATTAAATTATTAAATAAATACTAATT
ATATAGGGATTACATACAAT

Downstream 100 bases:

>100_bases
TTTAAATATATGCGCAGAATTTTATCAATATTATTAGAAAATGAATCAGGTGCATTATCACGAGTAGTAGGACTTTTTTC
ACAAAGAGGATATAATATAG

Product: IlvI

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 574; Mature: 573

Protein sequence:

>574_residues
MTLLSGAEMVVQSLMNLKIKKIFGYPGGAVLDIYDAIHTLGKKKIQHILVRHEQAAVHMADGYARCTGKIGVVLVTSGPG
ATNAITGIATAYMDSIPLLIISGQVSSKLIGFDAFQECDMIGISRPIVKHSFLVKKSENIPLIFQKSYFLATNGRPGPIV
IDLPKNILDHKIKKKYIWENTINIKSYKNNIKKNKKNIKNALNFLLKSKKPIIYAGGGIISSNSHSELYNLATKLNIPVT
MSLMSLGSFPSNHPQNLSMLGMHGTYEANMAMHHSDIILAIGVRFDDRTTNNVDKYCPNAKIIHIDIDPTSISKTITADI
PIIGDAKKILKKMLLLLEKKEKKRSNSYLNNWWKKIQKWKNYKFNKYKNSNKIIKPQYILQILHKLTEGKAYITSDVGQH
QMFAALYYGFNKPRHWINSGGLGTMGFGLPAALGVKIAHPKKFVICVTGDGSIQMNIQELSTAKQYNLAILIINLNNQSL
GMVKQWQDMIYSGRHSHSYMQSLPNFIKLAKAYGHIGIKIKKPKEIISKLILAIKQVQSGSLVFVDINIDPYEHVYPMQI
KNGGMNEMLLKKTG

Sequences:

>Translated_574_residues
MTLLSGAEMVVQSLMNLKIKKIFGYPGGAVLDIYDAIHTLGKKKIQHILVRHEQAAVHMADGYARCTGKIGVVLVTSGPG
ATNAITGIATAYMDSIPLLIISGQVSSKLIGFDAFQECDMIGISRPIVKHSFLVKKSENIPLIFQKSYFLATNGRPGPIV
IDLPKNILDHKIKKKYIWENTINIKSYKNNIKKNKKNIKNALNFLLKSKKPIIYAGGGIISSNSHSELYNLATKLNIPVT
MSLMSLGSFPSNHPQNLSMLGMHGTYEANMAMHHSDIILAIGVRFDDRTTNNVDKYCPNAKIIHIDIDPTSISKTITADI
PIIGDAKKILKKMLLLLEKKEKKRSNSYLNNWWKKIQKWKNYKFNKYKNSNKIIKPQYILQILHKLTEGKAYITSDVGQH
QMFAALYYGFNKPRHWINSGGLGTMGFGLPAALGVKIAHPKKFVICVTGDGSIQMNIQELSTAKQYNLAILIINLNNQSL
GMVKQWQDMIYSGRHSHSYMQSLPNFIKLAKAYGHIGIKIKKPKEIISKLILAIKQVQSGSLVFVDINIDPYEHVYPMQI
KNGGMNEMLLKKTG
>Mature_573_residues
TLLSGAEMVVQSLMNLKIKKIFGYPGGAVLDIYDAIHTLGKKKIQHILVRHEQAAVHMADGYARCTGKIGVVLVTSGPGA
TNAITGIATAYMDSIPLLIISGQVSSKLIGFDAFQECDMIGISRPIVKHSFLVKKSENIPLIFQKSYFLATNGRPGPIVI
DLPKNILDHKIKKKYIWENTINIKSYKNNIKKNKKNIKNALNFLLKSKKPIIYAGGGIISSNSHSELYNLATKLNIPVTM
SLMSLGSFPSNHPQNLSMLGMHGTYEANMAMHHSDIILAIGVRFDDRTTNNVDKYCPNAKIIHIDIDPTSISKTITADIP
IIGDAKKILKKMLLLLEKKEKKRSNSYLNNWWKKIQKWKNYKFNKYKNSNKIIKPQYILQILHKLTEGKAYITSDVGQHQ
MFAALYYGFNKPRHWINSGGLGTMGFGLPAALGVKIAHPKKFVICVTGDGSIQMNIQELSTAKQYNLAILIINLNNQSLG
MVKQWQDMIYSGRHSHSYMQSLPNFIKLAKAYGHIGIKIKKPKEIISKLILAIKQVQSGSLVFVDINIDPYEHVYPMQIK
NGGMNEMLLKKTG

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=575, Percent_Identity=25.3913043478261, Blast_Score=179, Evalue=8e-45,
Organism=Homo sapiens, GI21361361, Length=589, Percent_Identity=22.4108658743633, Blast_Score=151, Evalue=2e-36,
Organism=Escherichia coli, GI87081685, Length=573, Percent_Identity=64.0488656195462, Blast_Score=822, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=567, Percent_Identity=37.2134038800705, Blast_Score=408, Evalue=1e-115,
Organism=Escherichia coli, GI1786717, Length=521, Percent_Identity=33.3973128598848, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI1787096, Length=555, Percent_Identity=26.1261261261261, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1788716, Length=567, Percent_Identity=22.0458553791887, Blast_Score=155, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI17542570, Length=585, Percent_Identity=23.7606837606838, Blast_Score=143, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17531299, Length=577, Percent_Identity=24.9566724436742, Blast_Score=142, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17531301, Length=577, Percent_Identity=24.9566724436742, Blast_Score=141, Evalue=9e-34,
Organism=Saccharomyces cerevisiae, GI6323755, Length=588, Percent_Identity=39.4557823129252, Blast_Score=431, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6320816, Length=486, Percent_Identity=25.7201646090535, Blast_Score=127, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6323163, Length=495, Percent_Identity=23.030303030303, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19922626, Length=498, Percent_Identity=26.1044176706827, Blast_Score=185, Evalue=9e-47,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 64189; Mature: 64057

Theoretical pI: Translated: 10.42; Mature: 10.42

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLSGAEMVVQSLMNLKIKKIFGYPGGAVLDIYDAIHTLGKKKIQHILVRHEQAAVHMA
CCCCCHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEC
DGYARCTGKIGVVLVTSGPGATNAITGIATAYMDSIPLLIISGQVSSKLIGFDAFQECDM
CCCEEECCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEECCHHHHHCCC
IGISRPIVKHSFLVKKSENIPLIFQKSYFLATNGRPGPIVIDLPKNILDHKIKKKYIWEN
CCCCCCHHHHHEEEECCCCCCEEEECCEEEEECCCCCCEEEECCHHHHHHHHHHHHHCCC
TINIKSYKNNIKKNKKNIKNALNFLLKSKKPIIYAGGGIISSNSHSELYNLATKLNIPVT
CEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEECCCCHHHHHHHHHHCCCCEE
MSLMSLGSFPSNHPQNLSMLGMHGTYEANMAMHHSDIILAIGVRFDDRTTNNVDKYCPNA
EEHHHHCCCCCCCCCCCEEEECCCCEECCEEEECCCEEEEEEEEECCCCCCCHHHHCCCC
KIIHIDIDPTSISKTITADIPIIGDAKKILKKMLLLLEKKEKKRSNSYLNNWWKKIQKWK
EEEEEECCCCCCCCEEEECCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NYKFNKYKNSNKIIKPQYILQILHKLTEGKAYITSDVGQHQMFAALYYGFNKPRHWINSG
CCCCCEECCCCCEECHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHCCCCCCHHHCCC
GLGTMGFGLPAALGVKIAHPKKFVICVTGDGSIQMNIQELSTAKQYNLAILIINLNNQSL
CCCCCCCCCHHHHCEEEECCCEEEEEEECCCCEEEEHHHHHCCCCCEEEEEEEEECCCCC
GMVKQWQDMIYSGRHSHSYMQSLPNFIKLAKAYGHIGIKIKKPKEIISKLILAIKQVQSG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHCCCC
SLVFVDINIDPYEHVYPMQIKNGGMNEMLLKKTG
CEEEEEECCCCCCCEEEEEECCCCCCCEEEECCC
>Mature Secondary Structure 
TLLSGAEMVVQSLMNLKIKKIFGYPGGAVLDIYDAIHTLGKKKIQHILVRHEQAAVHMA
CCCCHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEC
DGYARCTGKIGVVLVTSGPGATNAITGIATAYMDSIPLLIISGQVSSKLIGFDAFQECDM
CCCEEECCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEECCHHHHHCCC
IGISRPIVKHSFLVKKSENIPLIFQKSYFLATNGRPGPIVIDLPKNILDHKIKKKYIWEN
CCCCCCHHHHHEEEECCCCCCEEEECCEEEEECCCCCCEEEECCHHHHHHHHHHHHHCCC
TINIKSYKNNIKKNKKNIKNALNFLLKSKKPIIYAGGGIISSNSHSELYNLATKLNIPVT
CEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEECCCCHHHHHHHHHHCCCCEE
MSLMSLGSFPSNHPQNLSMLGMHGTYEANMAMHHSDIILAIGVRFDDRTTNNVDKYCPNA
EEHHHHCCCCCCCCCCCEEEECCCCEECCEEEECCCEEEEEEEEECCCCCCCHHHHCCCC
KIIHIDIDPTSISKTITADIPIIGDAKKILKKMLLLLEKKEKKRSNSYLNNWWKKIQKWK
EEEEEECCCCCCCCEEEECCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NYKFNKYKNSNKIIKPQYILQILHKLTEGKAYITSDVGQHQMFAALYYGFNKPRHWINSG
CCCCCEECCCCCEECHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHCCCCCCHHHCCC
GLGTMGFGLPAALGVKIAHPKKFVICVTGDGSIQMNIQELSTAKQYNLAILIINLNNQSL
CCCCCCCCCHHHHCEEEECCCEEEEEEECCCCEEEEHHHHHCCCCCEEEEEEEEECCCCC
GMVKQWQDMIYSGRHSHSYMQSLPNFIKLAKAYGHIGIKIKKPKEIISKLILAIKQVQSG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHCCCC
SLVFVDINIDPYEHVYPMQIKNGGMNEMLLKKTG
CEEEEEECCCCCCCEEEEEECCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9688822; 12089438 [H]