Click here to switch to the map view.

The map label for this gene is nfo [H]

Identifier: 116515028

GI number: 116515028

Start: 95902

End: 96774

Strand: Direct

Name: nfo [H]

Synonym: BCc_087

Alternate gene names: 116515028

Gene position: 95902-96774 (Clockwise)

Preceding gene: 116515025

Following gene: 116515029

Centisome position: 23.03

GC content: 22.22

Gene sequence:

>873_bases
TTGATAAAGTATATAGGAGTTCACTGTAATACATCTGGAGGATTAGAAAAATCTGTTTTACAAGCTAATAAATTAAATGC
TACAGCTTTTTCTTTTTTTGTAAAAAATCCTTTGAGATGGAATACTTTGCCATTACAACAAAGTACAATTAAGAGTTTTC
GTAAGTTTTGTAATAAATATAATTATTCAACATTACAAATATTGCCTCATAGTAGTTATTTAATTAATTTAGGACATCCA
GATAAAGAAAAAAAAAAAAAATCATGTAAAACTTTAATTGAAGAAATAAATTTTTGTAATTTATTAGGTTTAGTGATGAT
AAATATTCATCCGGGGAGTTCTTTAAAAAAAATAACAGAAAAAAAATGTTTAGAAAATATTTCGAATTCTATTAATTATA
TATTAAAAAAAACTAATAATATTATTATTGTTTTAGAAAATACATCCGGTCAAGGTGGAAATATGGGGTATTGTTTTGAA
CATTTAGCTTTTATTATCAATAAAGTTCGAAACAAATCAAGAATTGGAGTATGTCTTGATACATGTCATTTATTTGCTTC
TGGATATCAATTAAATAATAAAAATAGTTTTTTAAATATTTTTTATAGATTTCATGAATTAATAGGATTGAAATATTTAC
GTGGAATTCATTTAAATGATTCTAAAGGAAAATTTAATAGTAAATTAGATCGACATGAAAATTTAGGTTATGGAAATATT
AAAACAAGTGTTTTCTCAAAAATTGTACGAGTAATTGAGTTTAACAATATTCCTATAATTTTAGAAACAAAAGACGCAAG
TATTTGGAAAAAAGAAATTCAATGGTTAAAAAAACAATCATTATTACCCAAAATTAGTATTTTAAATTTCTAA

Upstream 100 bases:

>100_bases
GGAATATTTATATTTTTTAATTATATTAAAATTAATTTATGTATACTATATTTATTTTTTATTATTATAATATATGTATT
AAAAAGAGGACAATGAAATT

Downstream 100 bases:

>100_bases
ATTCTAATTTATATATAAAATATTAAGAACATTTTTAAAATTTTAATATTTTAAATATATCATTTAATATTTATAGAAAT
TTTTGATAAAAACAAATTTT

Product: hypothetical protein

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MIKYIGVHCNTSGGLEKSVLQANKLNATAFSFFVKNPLRWNTLPLQQSTIKSFRKFCNKYNYSTLQILPHSSYLINLGHP
DKEKKKKSCKTLIEEINFCNLLGLVMINIHPGSSLKKITEKKCLENISNSINYILKKTNNIIIVLENTSGQGGNMGYCFE
HLAFIINKVRNKSRIGVCLDTCHLFASGYQLNNKNSFLNIFYRFHELIGLKYLRGIHLNDSKGKFNSKLDRHENLGYGNI
KTSVFSKIVRVIEFNNIPIILETKDASIWKKEIQWLKKQSLLPKISILNF

Sequences:

>Translated_290_residues
MIKYIGVHCNTSGGLEKSVLQANKLNATAFSFFVKNPLRWNTLPLQQSTIKSFRKFCNKYNYSTLQILPHSSYLINLGHP
DKEKKKKSCKTLIEEINFCNLLGLVMINIHPGSSLKKITEKKCLENISNSINYILKKTNNIIIVLENTSGQGGNMGYCFE
HLAFIINKVRNKSRIGVCLDTCHLFASGYQLNNKNSFLNIFYRFHELIGLKYLRGIHLNDSKGKFNSKLDRHENLGYGNI
KTSVFSKIVRVIEFNNIPIILETKDASIWKKEIQWLKKQSLLPKISILNF
>Mature_290_residues
MIKYIGVHCNTSGGLEKSVLQANKLNATAFSFFVKNPLRWNTLPLQQSTIKSFRKFCNKYNYSTLQILPHSSYLINLGHP
DKEKKKKSCKTLIEEINFCNLLGLVMINIHPGSSLKKITEKKCLENISNSINYILKKTNNIIIVLENTSGQGGNMGYCFE
HLAFIINKVRNKSRIGVCLDTCHLFASGYQLNNKNSFLNIFYRFHELIGLKYLRGIHLNDSKGKFNSKLDRHENLGYGNI
KTSVFSKIVRVIEFNNIPIILETKDASIWKKEIQWLKKQSLLPKISILNF

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788483, Length=278, Percent_Identity=49.6402877697842, Blast_Score=310, Evalue=9e-86,
Organism=Caenorhabditis elegans, GI17531193, Length=264, Percent_Identity=40.530303030303, Blast_Score=244, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6322735, Length=281, Percent_Identity=38.4341637010676, Blast_Score=209, Evalue=4e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =3.1.21.2 [H]

Molecular weight: Translated: 33279; Mature: 33279

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: PS00729 AP_NUCLEASE_F2_1 ; PS00730 AP_NUCLEASE_F2_2 ; PS00731 AP_NUCLEASE_F2_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKYIGVHCNTSGGLEKSVLQANKLNATAFSFFVKNPLRWNTLPLQQSTIKSFRKFCNKY
CEEEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHC
NYSTLQILPHSSYLINLGHPDKEKKKKSCKTLIEEINFCNLLGLVMINIHPGSSLKKITE
CCCEEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCHHHHHHH
KKCLENISNSINYILKKTNNIIIVLENTSGQGGNMGYCFEHLAFIINKVRNKSRIGVCLD
HHHHHHHHHHHHHHEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHH
TCHLFASGYQLNNKNSFLNIFYRFHELIGLKYLRGIHLNDSKGKFNSKLDRHENLGYGNI
HHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHCEECCCCCCCHHHHHHHHCCCCCCCH
KTSVFSKIVRVIEFNNIPIILETKDASIWKKEIQWLKKQSLLPKISILNF
HHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEECCC
>Mature Secondary Structure
MIKYIGVHCNTSGGLEKSVLQANKLNATAFSFFVKNPLRWNTLPLQQSTIKSFRKFCNKY
CEEEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHC
NYSTLQILPHSSYLINLGHPDKEKKKKSCKTLIEEINFCNLLGLVMINIHPGSSLKKITE
CCCEEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCHHHHHHH
KKCLENISNSINYILKKTNNIIIVLENTSGQGGNMGYCFEHLAFIINKVRNKSRIGVCLD
HHHHHHHHHHHHHHEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHH
TCHLFASGYQLNNKNSFLNIFYRFHELIGLKYLRGIHLNDSKGKFNSKLDRHENLGYGNI
HHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHCEECCCCCCCHHHHHHHHCCCCCCCH
KTSVFSKIVRVIEFNNIPIILETKDASIWKKEIQWLKKQSLLPKISILNF
HHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA