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The map label for this gene is queA [H]

Identifier: 116332583

GI number: 116332583

Start: 177284

End: 178327

Strand: Direct

Name: queA [H]

Synonym: LBJ_4155

Alternate gene names: 116332583

Gene position: 177284-178327 (Clockwise)

Preceding gene: 116332573

Following gene: 116332584

Centisome position: 59.14

GC content: 39.56

Gene sequence:

>1044_bases
ATGTTGTTCGAAGATCTCAAAGAATTCGAGTTTACGTTGTCCGAAGAAAGGATCGCTCGTTATCCGGCGGCCAATCGGGA
CGAAAGCAGGTTGATGGTTCTTGACGTGAATGTTGGAAATATCTTCAGCGAATCTTCGTTTAAAAACCTTGTTTCCTATT
TGAAAGAGGGGGACGTTTTAGTGGCCAATCATACTAAGGTAAGCAAACGCAGAGTTTATTTAAAAACGCAAATTCGCACC
CACGAAACAATGTTTCTCGAAGAAAAAGAATTACTTTGGAAATGTAAAATTCGAAACTCCAAAAAATTGAAAGTAGGAAC
AAAACTTTCCGACGAAAAAACGCAACGTATCTCATTTACAGTGGAAAAAAAAGAAGGTGAATTCGTTTTTTTAAAACCGG
AACAAACTCTACAAGAAGAAGATTTTCAGCAAATAGGAGAAATTCCAATCCCGCCATATTTCAAAAGAAATCCAGACCCC
AAGGACGAAATTCGATACCAAACACTCTTTGCAAAAACTCCTGGTTCCGTTGCCGCTCCGACAGCAGGTTTGCATTTTTC
AGAATCCATCTTTCAAACCCTGAAAGAGAAAAAGATTCGATTCTGCACTTTAGAGCTCAAAGTTGGTTACGGAACCTTTC
AGCCCCTTACCGAAGAAAATTTTCAAAATCAAAAATTGCATCGGGAAGAATTCTTTCTGGAAAAATCGAGCACGGAAATT
TTGAATCTCGCTAAAAAAGAAGGAAGAAGAATTTTTTCGATCGGCACCACAACACTGCGAGCGCTTGAATCCGCTTATGA
CTACGCAACAAATTCTTTCCGTTCGGGACGCGGGGCCACGGAACTTTTTATTCAACCGGAAGATCGACTGAAAAGCTGCG
AATGTCTAATCACAAACTTTCATTTACCGAGTTCGAGTCTTCTTTTACTCGTTTCCGCATTTGCGGGAAAAGAACTGGTT
TTAAAAGCATATCAAAAAGCGATTCAAGAAAAATTTAGATTCCATTCTTACGGAGACGCGATGCTGATTTTAGGAAAACG
ATGA

Upstream 100 bases:

>100_bases
ATTATCTTCTGCGAAAGCGATATCCGGCAGTTTGCCGACGCTTCCATCCCGACAGAAAAAAGATTCTCCTGCAAACCGTT
TACGGATCCACTGCAAATAG

Downstream 100 bases:

>100_bases
TTGAACGGACCCATCCATACTTCTTCGAGAATCGATATGATGTTTCGTCAAATTAATATATTGATCATACAAGGAACCCG
ACGGAGAATCGTTTTTAGTG

Product: S-adenosylmethionine:tRNA-ribosyltransferase- isomerase (queuosine synthetase)

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MLFEDLKEFEFTLSEERIARYPAANRDESRLMVLDVNVGNIFSESSFKNLVSYLKEGDVLVANHTKVSKRRVYLKTQIRT
HETMFLEEKELLWKCKIRNSKKLKVGTKLSDEKTQRISFTVEKKEGEFVFLKPEQTLQEEDFQQIGEIPIPPYFKRNPDP
KDEIRYQTLFAKTPGSVAAPTAGLHFSESIFQTLKEKKIRFCTLELKVGYGTFQPLTEENFQNQKLHREEFFLEKSSTEI
LNLAKKEGRRIFSIGTTTLRALESAYDYATNSFRSGRGATELFIQPEDRLKSCECLITNFHLPSSSLLLLVSAFAGKELV
LKAYQKAIQEKFRFHSYGDAMLILGKR

Sequences:

>Translated_347_residues
MLFEDLKEFEFTLSEERIARYPAANRDESRLMVLDVNVGNIFSESSFKNLVSYLKEGDVLVANHTKVSKRRVYLKTQIRT
HETMFLEEKELLWKCKIRNSKKLKVGTKLSDEKTQRISFTVEKKEGEFVFLKPEQTLQEEDFQQIGEIPIPPYFKRNPDP
KDEIRYQTLFAKTPGSVAAPTAGLHFSESIFQTLKEKKIRFCTLELKVGYGTFQPLTEENFQNQKLHREEFFLEKSSTEI
LNLAKKEGRRIFSIGTTTLRALESAYDYATNSFRSGRGATELFIQPEDRLKSCECLITNFHLPSSSLLLLVSAFAGKELV
LKAYQKAIQEKFRFHSYGDAMLILGKR
>Mature_347_residues
MLFEDLKEFEFTLSEERIARYPAANRDESRLMVLDVNVGNIFSESSFKNLVSYLKEGDVLVANHTKVSKRRVYLKTQIRT
HETMFLEEKELLWKCKIRNSKKLKVGTKLSDEKTQRISFTVEKKEGEFVFLKPEQTLQEEDFQQIGEIPIPPYFKRNPDP
KDEIRYQTLFAKTPGSVAAPTAGLHFSESIFQTLKEKKIRFCTLELKVGYGTFQPLTEENFQNQKLHREEFFLEKSSTEI
LNLAKKEGRRIFSIGTTTLRALESAYDYATNSFRSGRGATELFIQPEDRLKSCECLITNFHLPSSSLLLLVSAFAGKELV
LKAYQKAIQEKFRFHSYGDAMLILGKR

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=345, Percent_Identity=36.5217391304348, Blast_Score=221, Evalue=5e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 40178; Mature: 40178

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFEDLKEFEFTLSEERIARYPAANRDESRLMVLDVNVGNIFSESSFKNLVSYLKEGDVL
CCHHHHHHHHHHHCHHHHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCEE
VANHTKVSKRRVYLKTQIRTHETMFLEEKELLWKCKIRNSKKLKVGTKLSDEKTQRISFT
EEECCCCCCEEEEEEEEECCCHHHEEEHHHHEEEEECCCCCEEEECCCCCCCCCEEEEEE
VEKKEGEFVFLKPEQTLQEEDFQQIGEIPIPPYFKRNPDPKDEIRYQTLFAKTPGSVAAP
EEECCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHEEEEEEECCCCCCCCC
TAGLHFSESIFQTLKEKKIRFCTLELKVGYGTFQPLTEENFQNQKLHREEFFLEKSSTEI
CCCCCHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHH
LNLAKKEGRRIFSIGTTTLRALESAYDYATNSFRSGRGATELFIQPEDRLKSCECLITNF
HHHHHHCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHCCCEEEEEEC
HLPSSSLLLLVSAFAGKELVLKAYQKAIQEKFRFHSYGDAMLILGKR
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
>Mature Secondary Structure
MLFEDLKEFEFTLSEERIARYPAANRDESRLMVLDVNVGNIFSESSFKNLVSYLKEGDVL
CCHHHHHHHHHHHCHHHHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCEE
VANHTKVSKRRVYLKTQIRTHETMFLEEKELLWKCKIRNSKKLKVGTKLSDEKTQRISFT
EEECCCCCCEEEEEEEEECCCHHHEEEHHHHEEEEECCCCCEEEECCCCCCCCCEEEEEE
VEKKEGEFVFLKPEQTLQEEDFQQIGEIPIPPYFKRNPDPKDEIRYQTLFAKTPGSVAAP
EEECCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHEEEEEEECCCCCCCCC
TAGLHFSESIFQTLKEKKIRFCTLELKVGYGTFQPLTEENFQNQKLHREEFFLEKSSTEI
CCCCCHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHH
LNLAKKEGRRIFSIGTTTLRALESAYDYATNSFRSGRGATELFIQPEDRLKSCECLITNF
HHHHHHCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHCCCEEEEEEC
HLPSSSLLLLVSAFAGKELVLKAYQKAIQEKFRFHSYGDAMLILGKR
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA