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The map label for this gene is rmlD [H]

Identifier: 116327947

GI number: 116327947

Start: 1454507

End: 1455427

Strand: Direct

Name: rmlD [H]

Synonym: LBL_1236

Alternate gene names: 116327947

Gene position: 1454507-1455427 (Clockwise)

Preceding gene: 116327946

Following gene: 116327948

Centisome position: 40.24

GC content: 35.83

Gene sequence:

>921_bases
ATGATTTATTATACTGGAAAAAGCGGTCAGTTAGGTTGGGAGTTGACCAAAAGATTCAAATCTTTGGATTTGGAATCTAT
AGGATTTAGTAGAGAAGAATGGGACCTTACCGATCTTGATTCTGTAGAAAAAATTTTAAAAGATTCTCCTAAAATTTTAG
TTCATTGTGGAGCCTATACTGCCGTGGATAAAGCAGAGTCCGATTCGGAGAACGTTTACAAGATCAATTCCTTATCTGTA
AAAAAAATTTCGGAAGAATGTCTAAAAAGAAAAATCCATCTGATTTATATATCCACTGATTTTGTGTTCGATGCAAATTC
GGAAACGATCGGGGATACGATTCGTTTTTGGAAACCGGATTCTACACTTTCCCCGAAAGGAATTTATGGTTTATCGAAAG
CGGAAGGAGAAAGGTGGATTCAAAATATATTTGCGGATTCTAAACAAGCGAATATAGTCCGAACAAGTTGGGTATATTCC
TCTTATGGAAATAATTTTCCTAAAACAATTTTGAAACTTTTACAAGATCCTAAGCGTACCGAATTAAAAGTAATTGAAGA
TCAATTGGGGAGGCCTACTTGGGCGGGTAGGCTTGCCGACTTTATAATATTCTTAATTTATGAAATTCTAAAAAAGGGGG
GGTATCCGGAGATTTTGCATTTTTCCAATTCCGGAATTGCGAGTTGGTATGATTTTGCGATTGCAATTCGAGATATCTCG
TATTCTTTTTCATTGATAGAAAATTTAAAACCGATTTCTCCGATTCCTACGGAAAGTTATCCTACAGCTGCCCCGAGACC
TAGATACTCAATTTTAGATTTAAATGAAACTCGAAAAATTTTTGGTCCCGTTCCGCATTGGAAAGAAGATTTAACTCTCT
GTCTGAAAGAAATCGCCGAGATTTTCGGAAAAAAAGTATGA

Upstream 100 bases:

>100_bases
TTGGAAAACCTGGTTGTCTGATTCTGAGTTTATAGTGTCTCAAAGGGATCAAGAAACTCCTTTTCTTGCGAGCTTTAAAA
GCCCATTCGTATATTAGAAT

Downstream 100 bases:

>100_bases
AAAAAATTTTAGTCACCGGTGGAGCAGGATTTATAGGTTCCAATTTTGTGAATCTCATTTTAAACGAAAATGAAGAATAT
CAGATAATCGTATTCGATAA

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MIYYTGKSGQLGWELTKRFKSLDLESIGFSREEWDLTDLDSVEKILKDSPKILVHCGAYTAVDKAESDSENVYKINSLSV
KKISEECLKRKIHLIYISTDFVFDANSETIGDTIRFWKPDSTLSPKGIYGLSKAEGERWIQNIFADSKQANIVRTSWVYS
SYGNNFPKTILKLLQDPKRTELKVIEDQLGRPTWAGRLADFIIFLIYEILKKGGYPEILHFSNSGIASWYDFAIAIRDIS
YSFSLIENLKPISPIPTESYPTAAPRPRYSILDLNETRKIFGPVPHWKEDLTLCLKEIAEIFGKKV

Sequences:

>Translated_306_residues
MIYYTGKSGQLGWELTKRFKSLDLESIGFSREEWDLTDLDSVEKILKDSPKILVHCGAYTAVDKAESDSENVYKINSLSV
KKISEECLKRKIHLIYISTDFVFDANSETIGDTIRFWKPDSTLSPKGIYGLSKAEGERWIQNIFADSKQANIVRTSWVYS
SYGNNFPKTILKLLQDPKRTELKVIEDQLGRPTWAGRLADFIIFLIYEILKKGGYPEILHFSNSGIASWYDFAIAIRDIS
YSFSLIENLKPISPIPTESYPTAAPRPRYSILDLNETRKIFGPVPHWKEDLTLCLKEIAEIFGKKV
>Mature_306_residues
MIYYTGKSGQLGWELTKRFKSLDLESIGFSREEWDLTDLDSVEKILKDSPKILVHCGAYTAVDKAESDSENVYKINSLSV
KKISEECLKRKIHLIYISTDFVFDANSETIGDTIRFWKPDSTLSPKGIYGLSKAEGERWIQNIFADSKQANIVRTSWVYS
SYGNNFPKTILKLLQDPKRTELKVIEDQLGRPTWAGRLADFIIFLIYEILKKGGYPEILHFSNSGIASWYDFAIAIRDIS
YSFSLIENLKPISPIPTESYPTAAPRPRYSILDLNETRKIFGPVPHWKEDLTLCLKEIAEIFGKKV

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Escherichia coli, GI1788352, Length=301, Percent_Identity=34.8837209302326, Blast_Score=157, Evalue=9e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 35012; Mature: 35012

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYYTGKSGQLGWELTKRFKSLDLESIGFSREEWDLTDLDSVEKILKDSPKILVHCGAYT
CEEECCCCCCCHHHHHHHHHHCCHHHCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCH
AVDKAESDSENVYKINSLSVKKISEECLKRKIHLIYISTDFVFDANSETIGDTIRFWKPD
HHHCCCCCCCCEEEECCCHHHHHHHHHHHCCEEEEEEEEEEEEECCCCCCCCCCEEECCC
STLSPKGIYGLSKAEGERWIQNIFADSKQANIVRTSWVYSSYGNNFPKTILKLLQDPKRT
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEHEEHHHHCCCCHHHHHHHHCCCCHH
ELKVIEDQLGRPTWAGRLADFIIFLIYEILKKGGYPEILHFSNSGIASWYDFAIAIRDIS
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHH
YSFSLIENLKPISPIPTESYPTAAPRPRYSILDLNETRKIFGPVPHWKEDLTLCLKEIAE
HHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHCCCCCCHHHHHHHHHHHHH
IFGKKV
HHCCCC
>Mature Secondary Structure
MIYYTGKSGQLGWELTKRFKSLDLESIGFSREEWDLTDLDSVEKILKDSPKILVHCGAYT
CEEECCCCCCCHHHHHHHHHHCCHHHCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCH
AVDKAESDSENVYKINSLSVKKISEECLKRKIHLIYISTDFVFDANSETIGDTIRFWKPD
HHHCCCCCCCCEEEECCCHHHHHHHHHHHCCEEEEEEEEEEEEECCCCCCCCCCEEECCC
STLSPKGIYGLSKAEGERWIQNIFADSKQANIVRTSWVYSSYGNNFPKTILKLLQDPKRT
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEHEEHHHHCCCCHHHHHHHHCCCCHH
ELKVIEDQLGRPTWAGRLADFIIFLIYEILKKGGYPEILHFSNSGIASWYDFAIAIRDIS
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHH
YSFSLIENLKPISPIPTESYPTAAPRPRYSILDLNETRKIFGPVPHWKEDLTLCLKEIAE
HHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHCCCCCCHHHHHHHHHHHHH
IFGKKV
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]