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The map label for this gene is rhmA [H]

Identifier: 116327911

GI number: 116327911

Start: 1412114

End: 1412869

Strand: Direct

Name: rhmA [H]

Synonym: LBL_1197

Alternate gene names: 116327911

Gene position: 1412114-1412869 (Clockwise)

Preceding gene: 116327910

Following gene: 116327912

Centisome position: 39.07

GC content: 38.23

Gene sequence:

>756_bases
ATGAATTCAGTGAAGGAAAAGCTTAAGTCTGGCGGATTTACAATTGGATCTTGGATGCAAATTCCAAATACTTCGATCGC
GGAAATCTTAGGAAGTGCTGGTTTTGATTGGATTGCTTTGGATTTAGAGCATGGTGCTTTTGGTTTGGATCTTCTTCCTG
ATATTTTTAGGGCGATCAAAATTGGCGGAAGTTTGCCCTTTGCAAGAGTTGCTCAGAATCATTCAAAGGATATCAAGCAG
GCTTTGGATGCAGGAGCTAAAGGTATTATCGTACCTATGATAGAAACTGCAAAACAAGTAGAAGATTTTCTGAAATGGAG
TAGTTATCCTCCGGACGGAACGAGAGGAGTGGGCTATTCAAATGCAAATCTATTTGGAAAATATTTCGATTCCGATTCGA
AAAAAATTAATTATGATTTAGTTCGTGTGATTCAAATCGAAAATATCAAAGCGATCCAACAAATTGATTCGATACTTTCT
GTAAAAGGAATCGATGTCATGATGATTGGTCCCTACGATTTGTCCGGTTCAATGGGATTGACCGGCCAATTTGGACATCC
TGATTTTTTACATGCAATTGGAGAACTTCATAAGGCAGCAGAACGGAATGGCATTCCTCTCGGGACGCATGTCATTCAAG
CGGATTTTCAAAAAGTAAAAGATGAAATTCGTGCCGGTAAGAAGTTTGTCGCCTATTCTACGGATGCGATTATGTTAAAT
ACGCAAGCTAATTTGAATTTTGAAGAATTAATATAA

Upstream 100 bases:

>100_bases
TTTGTACCTTCTGAAAAAGGAAAGTATGGAATTATAGAAGGAGTTCATGAGGTCATTCATCATTTGCTCTATGAGTTTGC
GGTTTTATTAGAAAAATAAT

Downstream 100 bases:

>100_bases
TTTAAAGTTATTAATTACTAATTTTGGAATAAAAATATGAAAACGATTGCTGTGTTGCCGGCGAGAATGGCATCTTCCAG
ATTTCCCGATAAACCATTGG

Product: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Products: pyruvate; tartronate semialdehyde

Alternate protein names: KDR aldolase; 2-dehydro-3-deoxyrhamnonate aldolase [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MNSVKEKLKSGGFTIGSWMQIPNTSIAEILGSAGFDWIALDLEHGAFGLDLLPDIFRAIKIGGSLPFARVAQNHSKDIKQ
ALDAGAKGIIVPMIETAKQVEDFLKWSSYPPDGTRGVGYSNANLFGKYFDSDSKKINYDLVRVIQIENIKAIQQIDSILS
VKGIDVMMIGPYDLSGSMGLTGQFGHPDFLHAIGELHKAAERNGIPLGTHVIQADFQKVKDEIRAGKKFVAYSTDAIMLN
TQANLNFEELI

Sequences:

>Translated_251_residues
MNSVKEKLKSGGFTIGSWMQIPNTSIAEILGSAGFDWIALDLEHGAFGLDLLPDIFRAIKIGGSLPFARVAQNHSKDIKQ
ALDAGAKGIIVPMIETAKQVEDFLKWSSYPPDGTRGVGYSNANLFGKYFDSDSKKINYDLVRVIQIENIKAIQQIDSILS
VKGIDVMMIGPYDLSGSMGLTGQFGHPDFLHAIGELHKAAERNGIPLGTHVIQADFQKVKDEIRAGKKFVAYSTDAIMLN
TQANLNFEELI
>Mature_251_residues
MNSVKEKLKSGGFTIGSWMQIPNTSIAEILGSAGFDWIALDLEHGAFGLDLLPDIFRAIKIGGSLPFARVAQNHSKDIKQ
ALDAGAKGIIVPMIETAKQVEDFLKWSSYPPDGTRGVGYSNANLFGKYFDSDSKKINYDLVRVIQIENIKAIQQIDSILS
VKGIDVMMIGPYDLSGSMGLTGQFGHPDFLHAIGELHKAAERNGIPLGTHVIQADFQKVKDEIRAGKKFVAYSTDAIMLN
TQANLNFEELI

Specific function: Catalyzes the reversible retro-aldol cleavage of 2-keto- 3-deoxy-L-rhamnonate (KDR) to pyruvate and lactaldehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. KDR aldolase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=236, Percent_Identity=33.0508474576271, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1788578, Length=239, Percent_Identity=32.2175732217573, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.2.20

Molecular weight: Translated: 27443; Mature: 27443

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSVKEKLKSGGFTIGSWMQIPNTSIAEILGSAGFDWIALDLEHGAFGLDLLPDIFRAIK
CCHHHHHHHHCCCEEECEEECCCCHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHH
IGGSLPFARVAQNHSKDIKQALDAGAKGIIVPMIETAKQVEDFLKWSSYPPDGTRGVGYS
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
NANLFGKYFDSDSKKINYDLVRVIQIENIKAIQQIDSILSVKGIDVMMIGPYDLSGSMGL
CCCHHHHHHCCCCCEECCEEEEEEEECCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
TGQFGHPDFLHAIGELHKAAERNGIPLGTHVIQADFQKVKDEIRAGKKFVAYSTDAIMLN
CCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEE
TQANLNFEELI
CCCCCCHHHCC
>Mature Secondary Structure
MNSVKEKLKSGGFTIGSWMQIPNTSIAEILGSAGFDWIALDLEHGAFGLDLLPDIFRAIK
CCHHHHHHHHCCCEEECEEECCCCHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHH
IGGSLPFARVAQNHSKDIKQALDAGAKGIIVPMIETAKQVEDFLKWSSYPPDGTRGVGYS
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
NANLFGKYFDSDSKKINYDLVRVIQIENIKAIQQIDSILSVKGIDVMMIGPYDLSGSMGL
CCCHHHHHHCCCCCEECCEEEEEEEECCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
TGQFGHPDFLHAIGELHKAAERNGIPLGTHVIQADFQKVKDEIRAGKKFVAYSTDAIMLN
CCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEE
TQANLNFEELI
CCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-dehydro-3-deoxy-D-glucarate

Specific reaction: 2-dehydro-3-deoxy-D-glucarate = pyruvate + tartronate semialdehyde

General reaction: Carbon-Carbon Lyases; Aldehyde-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA