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The map label for this gene is proW [H]

Identifier: 116249179

GI number: 116249179

Start: 560100

End: 560987

Strand: Reverse

Name: proW [H]

Synonym: pRL120515

Alternate gene names: 116249179

Gene position: 560987-560100 (Counterclockwise)

Preceding gene: 116249180

Following gene: 116249178

Centisome position: 64.48

GC content: 58.67

Gene sequence:

>888_bases
ATGGAATGGTTTTATAAATTCCCGCATATGGATGACGACGCCCTTCGCAATCTGAAGAAGGCGATCGATGACGGTTTTCG
CGCATTTACCCGCACCTATGGCGACGCGATCGAAAGCCTCTTCTCCCCGCTGCAGCATTTCCTGATCGCCGCCGACCGCT
TCATGACGCAGACGCCGTGGCCGATCATTACGGCAATCATCCTTGTCATCGCATGGTTTGCCAGCCGCAGCCTGAAGGTC
GTCCTCGGCTGTCTCGTTACACTGCTGCTGATCGGCTATTTCGACATGTGGGACGATACGATGCGGACGGTCTCGATGAT
CTTCGTCTGTACCGTGCTGTCCATCGCCATCGGCATTCCGATGGGGATATTGATGGCGCGTTCCGACCGGCTGCAGCGCA
TCATCAATCCGATCCTCGACGTCATGCAGACCATGCCGAGCTTCGTCTATCTGATCCCTGTCGTCATGCTGCTCGGCATC
GGCAAGGTGCCGGGACTGATCGCCGTCGTCATCTACGCCATCCCGCCGATGATAAGGCTGACCGACCTCGGCATCCGCCT
GGTCGACAAGGACGTGCTCGAGGCCGCCGATGCGTTCGGAACGTCGAGTTCGCAGAAGCTGTTCAAGGTCCAGCTGCCGC
TGGCGCTTCCCACTATCATGGCCGGCATCAACCAGACCATCATGATGGCGCTCGCCATGGTCGTCGTCGCTTCGATGATC
GGCGTCCAGGGTCTTGGCCAGCCGGTTCTGAAGGCCATCGCCAACCAGTACTTCACCCTGGGTATTTTCAACGGCCTTGC
CATCGTCGGCATTGCCATCATCTTCGACCGGGTCAGCCAGGCGTATGGCAAGCGGCTCCAGAAGCATCGGGAGATCGTCC
ATGGCTGA

Upstream 100 bases:

>100_bases
TCTAACGCCGACTATTGCGGTGCGCTTGATGCGCACCGCCTTTCGCTGCCGATAAAATCAAAAAGACTGCCACGGCTCTT
TGAAGAAAAAGGGGAACCGA

Downstream 100 bases:

>100_bases
TCAACGTTTCGGCGGCATCAAGATCCGCCACCTCTACAAGATCTTCGGTCCCAATCCTGGCGCTCATGTCGATGCCGTCA
AAAAAGGATTGTCGAAGACC

Product: putative permease component of ABC transporter

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MEWFYKFPHMDDDALRNLKKAIDDGFRAFTRTYGDAIESLFSPLQHFLIAADRFMTQTPWPIITAIILVIAWFASRSLKV
VLGCLVTLLLIGYFDMWDDTMRTVSMIFVCTVLSIAIGIPMGILMARSDRLQRIINPILDVMQTMPSFVYLIPVVMLLGI
GKVPGLIAVVIYAIPPMIRLTDLGIRLVDKDVLEAADAFGTSSSQKLFKVQLPLALPTIMAGINQTIMMALAMVVVASMI
GVQGLGQPVLKAIANQYFTLGIFNGLAIVGIAIIFDRVSQAYGKRLQKHREIVHG

Sequences:

>Translated_295_residues
MEWFYKFPHMDDDALRNLKKAIDDGFRAFTRTYGDAIESLFSPLQHFLIAADRFMTQTPWPIITAIILVIAWFASRSLKV
VLGCLVTLLLIGYFDMWDDTMRTVSMIFVCTVLSIAIGIPMGILMARSDRLQRIINPILDVMQTMPSFVYLIPVVMLLGI
GKVPGLIAVVIYAIPPMIRLTDLGIRLVDKDVLEAADAFGTSSSQKLFKVQLPLALPTIMAGINQTIMMALAMVVVASMI
GVQGLGQPVLKAIANQYFTLGIFNGLAIVGIAIIFDRVSQAYGKRLQKHREIVHG
>Mature_295_residues
MEWFYKFPHMDDDALRNLKKAIDDGFRAFTRTYGDAIESLFSPLQHFLIAADRFMTQTPWPIITAIILVIAWFASRSLKV
VLGCLVTLLLIGYFDMWDDTMRTVSMIFVCTVLSIAIGIPMGILMARSDRLQRIINPILDVMQTMPSFVYLIPVVMLLGI
GKVPGLIAVVIYAIPPMIRLTDLGIRLVDKDVLEAADAFGTSSSQKLFKVQLPLALPTIMAGINQTIMMALAMVVVASMI
GVQGLGQPVLKAIANQYFTLGIFNGLAIVGIAIIFDRVSQAYGKRLQKHREIVHG

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine/L-proline [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=276, Percent_Identity=43.4782608695652, Blast_Score=214, Evalue=6e-57,
Organism=Escherichia coli, GI1788451, Length=199, Percent_Identity=31.1557788944724, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788449, Length=189, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786564, Length=158, Percent_Identity=27.8481012658228, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32707; Mature: 32707

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2649479; 9205837; 9278503 [H]