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The map label for this gene is thcR [H]

Identifier: 116249175

GI number: 116249175

Start: 555954

End: 556955

Strand: Reverse

Name: thcR [H]

Synonym: pRL120511

Alternate gene names: 116249175

Gene position: 556955-555954 (Counterclockwise)

Preceding gene: 116249176

Following gene: 116249167

Centisome position: 64.02

GC content: 59.08

Gene sequence:

>1002_bases
ATGACGGAAAATAGCGCTGCGCCATTTTCATTTCGATTTAGCGGTTCGTCCTTCGACAACATGGTCGAAACGCTCGGGGG
GGCTTTTGGCGCATTTGATGCAGAGCCTGTCGGCCGCGCCCAGGACTTCCATTGGGGATTGGATTTCTCGGTATGTGAAA
AAGCCGTCCTACTCACCGGTTATCATGAGGCGGAGTTTCAGTTCAATATCGAGCCGACCGCCGATACGGCGGAGTATTTG
TCGATCGTCGTGCCGCGCAGCGGCGGCATGGGGGTAACCTATGGATCGTCCCGCATCGCCGAGGCCGGGCAAGGAAAATT
GCTTCTCTATAACAATTTCGAACCCAACAGCGTCATCATGCATGGGCAATCGAACGTCATCGACGAGTTGCTGATCAACT
GGCCCGTCATCCTGCAGACGATCGGCCAGACGTTCGAGATGCCGTTCAGCGGCTCCCTCGACCTGCTGCCGGAACTGGAT
CTGTCCAAGCCTGAAGGCCGGTTGATCGGCAATCTCACGGAGACGATCATCTCAGGCATGCGCGACGATGGCCCCTTGCT
GCAGTCGCCGATCGCCATGGCGCATATGACGCAGGCGCTCGCCGATCTGGTCGTGCGCCTGGTGCCGCACCGGCTGTCGC
ATTTCCTGCAGAATGGCCCGGCCCTGATCGCGCCCCGGCATGTCCGCCGAGCGATCGAATTCATGCACACCAATATCGAT
CAGCCGATCACCATGCCGAAGGTGGCCGAAGCGGCCGGCGTATCCAGCCGGGCGCTCGAAACCGGTTTTCGTGCGTTCAG
AGGAACCTCTCCCGCCGCCTATCTGCTGACGCTTCGGCTACGTGCCGCGCGCCAGGAACTGCTCGATCCCGAAAGCAAGG
AGACCATGAAGGCCGTCTGCCTCAAATGGGGCTTCTTTCATTTCGGCCGGTTTTCCGCAGTCTATAAAACCACATATGGA
GAATACCCCTCCGACACCAGGAAGCGCGTGATCCGCCGCTAG

Upstream 100 bases:

>100_bases
TCCAAGGCCGCTCATCCGGCTCTGATGAAGCGCCGCGCGCTGGCCTGCGAGCGGTGGAGAAGGGTATGTCGAAACAACCA
CATCGAACAATGGGAGCAGG

Downstream 100 bases:

>100_bases
CGGCCGTGGCCTGCGGCGCGGAGGTGTTTACGTCTCGTCCCGCGGCGGCAATGTGTAGTGCACTCTCATCGTCCTTGGAT
CCCGCTTCACGATCTGCAGC

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MTENSAAPFSFRFSGSSFDNMVETLGGAFGAFDAEPVGRAQDFHWGLDFSVCEKAVLLTGYHEAEFQFNIEPTADTAEYL
SIVVPRSGGMGVTYGSSRIAEAGQGKLLLYNNFEPNSVIMHGQSNVIDELLINWPVILQTIGQTFEMPFSGSLDLLPELD
LSKPEGRLIGNLTETIISGMRDDGPLLQSPIAMAHMTQALADLVVRLVPHRLSHFLQNGPALIAPRHVRRAIEFMHTNID
QPITMPKVAEAAGVSSRALETGFRAFRGTSPAAYLLTLRLRAARQELLDPESKETMKAVCLKWGFFHFGRFSAVYKTTYG
EYPSDTRKRVIRR

Sequences:

>Translated_333_residues
MTENSAAPFSFRFSGSSFDNMVETLGGAFGAFDAEPVGRAQDFHWGLDFSVCEKAVLLTGYHEAEFQFNIEPTADTAEYL
SIVVPRSGGMGVTYGSSRIAEAGQGKLLLYNNFEPNSVIMHGQSNVIDELLINWPVILQTIGQTFEMPFSGSLDLLPELD
LSKPEGRLIGNLTETIISGMRDDGPLLQSPIAMAHMTQALADLVVRLVPHRLSHFLQNGPALIAPRHVRRAIEFMHTNID
QPITMPKVAEAAGVSSRALETGFRAFRGTSPAAYLLTLRLRAARQELLDPESKETMKAVCLKWGFFHFGRFSAVYKTTYG
EYPSDTRKRVIRR
>Mature_332_residues
TENSAAPFSFRFSGSSFDNMVETLGGAFGAFDAEPVGRAQDFHWGLDFSVCEKAVLLTGYHEAEFQFNIEPTADTAEYLS
IVVPRSGGMGVTYGSSRIAEAGQGKLLLYNNFEPNSVIMHGQSNVIDELLINWPVILQTIGQTFEMPFSGSLDLLPELDL
SKPEGRLIGNLTETIISGMRDDGPLLQSPIAMAHMTQALADLVVRLVPHRLSHFLQNGPALIAPRHVRRAIEFMHTNIDQ
PITMPKVAEAAGVSSRALETGFRAFRGTSPAAYLLTLRLRAARQELLDPESKETMKAVCLKWGFFHFGRFSAVYKTTYGE
YPSDTRKRVIRR

Specific function: Probably involved in the positive regulation of the thc operon for the degradation of the thiocarbamate herbicide EPTC [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788778, Length=108, Percent_Identity=36.1111111111111, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 36848; Mature: 36716

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTENSAAPFSFRFSGSSFDNMVETLGGAFGAFDAEPVGRAQDFHWGLDFSVCEKAVLLTG
CCCCCCCCEEEEECCCCHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHEEC
YHEAEFQFNIEPTADTAEYLSIVVPRSGGMGVTYGSSRIAEAGQGKLLLYNNFEPNSVIM
CCCCEEEEECCCCCCHHHHEEEEEECCCCCEEEECCHHHHHCCCCCEEEEECCCCCEEEE
HGQSNVIDELLINWPVILQTIGQTFEMPFSGSLDLLPELDLSKPEGRLIGNLTETIISGM
ECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
RDDGPLLQSPIAMAHMTQALADLVVRLVPHRLSHFLQNGPALIAPRHVRRAIEFMHTNID
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHCCCC
QPITMPKVAEAAGVSSRALETGFRAFRGTSPAAYLLTLRLRAARQELLDPESKETMKAVC
CCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LKWGFFHFGRFSAVYKTTYGEYPSDTRKRVIRR
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCC
>Mature Secondary Structure 
TENSAAPFSFRFSGSSFDNMVETLGGAFGAFDAEPVGRAQDFHWGLDFSVCEKAVLLTG
CCCCCCCEEEEECCCCHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHEEC
YHEAEFQFNIEPTADTAEYLSIVVPRSGGMGVTYGSSRIAEAGQGKLLLYNNFEPNSVIM
CCCCEEEEECCCCCCHHHHEEEEEECCCCCEEEECCHHHHHCCCCCEEEEECCCCCEEEE
HGQSNVIDELLINWPVILQTIGQTFEMPFSGSLDLLPELDLSKPEGRLIGNLTETIISGM
ECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
RDDGPLLQSPIAMAHMTQALADLVVRLVPHRLSHFLQNGPALIAPRHVRRAIEFMHTNID
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHCCCC
QPITMPKVAEAAGVSSRALETGFRAFRGTSPAAYLLTLRLRAARQELLDPESKETMKAVC
CCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LKWGFFHFGRFSAVYKTTYGEYPSDTRKRVIRR
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]