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The map label for this gene is braD [H]

Identifier: 116249154

GI number: 116249154

Start: 532972

End: 533880

Strand: Reverse

Name: braD [H]

Synonym: pRL120490

Alternate gene names: 116249154

Gene position: 533880-532972 (Counterclockwise)

Preceding gene: 116249155

Following gene: 116249153

Centisome position: 61.36

GC content: 65.35

Gene sequence:

>909_bases
GTGATCAACACCCTGCTCCAGGGCATTCTGCTCGGCGGTTACTACGCCGTTATCGCCTGCGGCCTGTCCTTCATGTTCTC
GGTCATGCGGATCATCAATCTCGCGCATGGCAGCCTGGCGGTCGCAGCCGCTTACGGGCTGTGGCTGCTGGCCGCCAAAG
CCGGCATTCCGCCCTTCGCCGGCCTGCTGATCGTGCTGCCGGTCATGGCCGCGGTCGGCTGGCTGTTGCAGCGCTTCATT
CTGGAACGCAGCGCCCGCGGCGGCACGCTGCTGCCGATCCTCACGACCTTCGGCCTGTCGATCGTCATCGACAACCTGCT
GTTCGAGCAGTTCGGCGCCGACACCCGCTCGCTCGCGCCGTTCATCGGCAACCTGTCCTATGCTTCCTGGCAACTGCCGG
GCCACATCTACGTCGGCAAACTTGCCGTCATGATGATGGTGACGGCAGTCCTTATTCTCGGCGGGCTGCAGTTCTTCCTG
AGCCGCTTTGCGCTCGGTCGCGCGATCCGCGCCACGGCGGAAGATCCCGATACGGCGGGGCTGGTCGGCATCGATGCCCG
CAGGGTCAACGCCGTCGCCACCGCAATCACCATGGTCACGGTCGGGATCGCCGGTGCTTTTCTGGGCATGCGGGCAACCT
TCAGCGCCTATTCCGGCGGCCCGCAGCTTCTCTTCGCCTTCGAGGCGGCCGTCATCGGCGGAGCGGGATCGCTATGGGGC
ACACTTGCCGGCGGCATCGTTCTCGGCCTTGCCCAATCGCTCGGCGCCCAATTGCATCCGCAAGGCTTCCTGATCGGCGG
CCATGCCGTGTTCCTGCTGGTGCTTTTCGTCAGGCTATCCCGGTTCGGCATGCCGGTTCTCGACAAACTTGCCGCCTCCC
TGCGCACCCGTCTCCGGAGCCCGACATGA

Upstream 100 bases:

>100_bases
GACGCATCGTCCTCGATCGGCCGGCCGCCGCCGTCACGCGCGAACATGTCACTCAAGCCTATTTCGGATTGCACCGGACA
TCAGGCGAAAGGAGCGCATC

Downstream 100 bases:

>100_bases
GCCCCAACGCGACCCTTGCCTCCAACGGAATATCGATAGAACGCTGGACGAAATCGTCACGGGTGGCGCTCGGCTGCATG
GCCGCGGTGCTCGTCCTGCT

Product: putative permease component of ABC transporter

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MINTLLQGILLGGYYAVIACGLSFMFSVMRIINLAHGSLAVAAAYGLWLLAAKAGIPPFAGLLIVLPVMAAVGWLLQRFI
LERSARGGTLLPILTTFGLSIVIDNLLFEQFGADTRSLAPFIGNLSYASWQLPGHIYVGKLAVMMMVTAVLILGGLQFFL
SRFALGRAIRATAEDPDTAGLVGIDARRVNAVATAITMVTVGIAGAFLGMRATFSAYSGGPQLLFAFEAAVIGGAGSLWG
TLAGGIVLGLAQSLGAQLHPQGFLIGGHAVFLLVLFVRLSRFGMPVLDKLAASLRTRLRSPT

Sequences:

>Translated_302_residues
MINTLLQGILLGGYYAVIACGLSFMFSVMRIINLAHGSLAVAAAYGLWLLAAKAGIPPFAGLLIVLPVMAAVGWLLQRFI
LERSARGGTLLPILTTFGLSIVIDNLLFEQFGADTRSLAPFIGNLSYASWQLPGHIYVGKLAVMMMVTAVLILGGLQFFL
SRFALGRAIRATAEDPDTAGLVGIDARRVNAVATAITMVTVGIAGAFLGMRATFSAYSGGPQLLFAFEAAVIGGAGSLWG
TLAGGIVLGLAQSLGAQLHPQGFLIGGHAVFLLVLFVRLSRFGMPVLDKLAASLRTRLRSPT
>Mature_302_residues
MINTLLQGILLGGYYAVIACGLSFMFSVMRIINLAHGSLAVAAAYGLWLLAAKAGIPPFAGLLIVLPVMAAVGWLLQRFI
LERSARGGTLLPILTTFGLSIVIDNLLFEQFGADTRSLAPFIGNLSYASWQLPGHIYVGKLAVMMMVTAVLILGGLQFFL
SRFALGRAIRATAEDPDTAGLVGIDARRVNAVATAITMVTVGIAGAFLGMRATFSAYSGGPQLLFAFEAAVIGGAGSLWG
TLAGGIVLGLAQSLGAQLHPQGFLIGGHAVFLLVLFVRLSRFGMPVLDKLAASLRTRLRSPT

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=312, Percent_Identity=27.8846153846154, Blast_Score=84, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 31663; Mature: 31663

Theoretical pI: Translated: 10.85; Mature: 10.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINTLLQGILLGGYYAVIACGLSFMFSVMRIINLAHGSLAVAAAYGLWLLAAKAGIPPFA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHH
GLLIVLPVMAAVGWLLQRFILERSARGGTLLPILTTFGLSIVIDNLLFEQFGADTRSLAP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
FIGNLSYASWQLPGHIYVGKLAVMMMVTAVLILGGLQFFLSRFALGRAIRATAEDPDTAG
HHCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
LVGIDARRVNAVATAITMVTVGIAGAFLGMRATFSAYSGGPQLLFAFEAAVIGGAGSLWG
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHH
TLAGGIVLGLAQSLGAQLHPQGFLIGGHAVFLLVLFVRLSRFGMPVLDKLAASLRTRLRS
HHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
PT
CC
>Mature Secondary Structure
MINTLLQGILLGGYYAVIACGLSFMFSVMRIINLAHGSLAVAAAYGLWLLAAKAGIPPFA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHH
GLLIVLPVMAAVGWLLQRFILERSARGGTLLPILTTFGLSIVIDNLLFEQFGADTRSLAP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
FIGNLSYASWQLPGHIYVGKLAVMMMVTAVLILGGLQFFLSRFALGRAIRATAEDPDTAG
HHCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
LVGIDARRVNAVATAITMVTVGIAGAFLGMRATFSAYSGGPQLLFAFEAAVIGGAGSLWG
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHH
TLAGGIVLGLAQSLGAQLHPQGFLIGGHAVFLLVLFVRLSRFGMPVLDKLAASLRTRLRS
HHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
PT
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]