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The map label for this gene is tnaA

Identifier: 114800287

GI number: 114800287

Start: 829662

End: 831026

Strand: Reverse

Name: tnaA

Synonym: HNE_0810

Alternate gene names: 114800287

Gene position: 831026-829662 (Counterclockwise)

Preceding gene: 114798051

Following gene: 114799321

Centisome position: 22.43

GC content: 62.56

Gene sequence:

>1365_bases
ATGAAGACCATTATCGAACCTTTCCGCATCAAATCGGTTGAGCCGATCCGCATGACAACCCGAGAGGAACGCGCGCGCCT
GTTGGAGGCGGCGGGCTTTAACCTGTTCAAGCTGCATTCGGATGATGTGATCATCGATTTGCTGACGGACAGTGGCACCT
CGGCCATGAGCGCGGCCCAGTGGGGCGCTGTGATGACGGGAGACGAAAGCTATGCCGGGGCGCCCAGCTTCTACCGCTTC
GAGGCGGCGGTGAAAGACCTGATGGATTTCACCCACATCATCCCCGCTCACCAGGGCCGGGCGGCCGAACACCTGCTCTT
TAGCCTGATCGCCAAAACAGGCGACCTCATCCCCTCCAATACCCATTTCGATACAACGCGCGGGAATATCGAAGCGATGG
GCGCAGAGGCGCTTGACCTGCCGATTGCGGAGGGCCGCGTGCCTTCGCTCGACCATCCGTTCAAGGGCAATATGGATCTC
GCCGCGCTGGAGCGTGTGCTGACCGAAGAGGGCGGCCGCATTCCGGCCGTGATGATGACCATCACCAACAATGCCGGGGG
CGGGCAGCCTGTCAGCCTCGAAAACATCCGCGGCGCGGCCAGGCTCGCCAAAGCCCATGGCAAGGCGTTCTATATTGACG
GCTGCCGCTTTGCCGAGAATGCCTGGTTCATCAAGCTGCGCGAGGAGGGTCAGAAGGACCGCTCAATCAAGGAGATTGTG
CGCGAGACGTTTGCCCTGGCCGATGGCATGACGATGAGCGCCAAGAAGGACGCCTTCGCCAATATCGGCGGCTGGCTGGC
GCTGAACAATGACGCGCTCGCCGAGCGCGCCCGCACGCTGCTGATCCAGACTGAAGGCTTCCCCACTTATGGCGGCCTGG
CGGGGCGCGATCTGGACGCCATTGCCCAGGGCCTGAAGGAAATCATTGATGAGGACTATCTGCGCTACCGGGTACGGACG
AACGCTTACATCGCCGAACGGCTGGACGCGATGGGCGTGCCGGTCGTGAAACCAGCGGGCGGCCACGCCGTGTTCATCGA
CGCACGCGCTTTCCTCCCGCATATTCCGCCGCTGGAGTATCCTGGCCAGGCGCTGACCTGCGCGATGTATGAAACCGGCG
GCATCCGGGCCTGCGAGATCGGCACCGTCATGTTCGGCCGCAAGCCCGATGGTACCGAAGCGCCCGGCGCCATGGACCTC
GTGCGCCTCGCCATGCCCCGCCGCGTCTACACGCAGAGCCATGCGGACTATGTAGTGGAAGTGCTTGAAGACGTTGCCGC
GACGAAGGACACGCTCAAAGGCCTGCGCATTGTGAAAGAGCCCCCGATGATGCGCCACTTCACGGCGGCGTTTGAGCGGC
TTTAA

Upstream 100 bases:

>100_bases
CGAGGATTCCGGAGGGGCAACCGCAGCGCAGAGGTTCCTGTGCGCGGGGCATGCCATCAAGGCCCCACTCCCAGGACCGT
TTCAGCCGGGAGTGAGCCCT

Downstream 100 bases:

>100_bases
AGAAAGCCGGGCTGGAAAAGCCCGGCTTTCTTCATATCGAGCTTGAAGTTCCAGATCTACCGAACCGCAAAATCCGTCGG
CTCTGTGCTCTTGCCCTTTG

Product: tryptophanase

Products: NA

Alternate protein names: L-tryptophan indole-lyase; TNase

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MKTIIEPFRIKSVEPIRMTTREERARLLEAAGFNLFKLHSDDVIIDLLTDSGTSAMSAAQWGAVMTGDESYAGAPSFYRF
EAAVKDLMDFTHIIPAHQGRAAEHLLFSLIAKTGDLIPSNTHFDTTRGNIEAMGAEALDLPIAEGRVPSLDHPFKGNMDL
AALERVLTEEGGRIPAVMMTITNNAGGGQPVSLENIRGAARLAKAHGKAFYIDGCRFAENAWFIKLREEGQKDRSIKEIV
RETFALADGMTMSAKKDAFANIGGWLALNNDALAERARTLLIQTEGFPTYGGLAGRDLDAIAQGLKEIIDEDYLRYRVRT
NAYIAERLDAMGVPVVKPAGGHAVFIDARAFLPHIPPLEYPGQALTCAMYETGGIRACEIGTVMFGRKPDGTEAPGAMDL
VRLAMPRRVYTQSHADYVVEVLEDVAATKDTLKGLRIVKEPPMMRHFTAAFERL

Sequences:

>Translated_454_residues
MKTIIEPFRIKSVEPIRMTTREERARLLEAAGFNLFKLHSDDVIIDLLTDSGTSAMSAAQWGAVMTGDESYAGAPSFYRF
EAAVKDLMDFTHIIPAHQGRAAEHLLFSLIAKTGDLIPSNTHFDTTRGNIEAMGAEALDLPIAEGRVPSLDHPFKGNMDL
AALERVLTEEGGRIPAVMMTITNNAGGGQPVSLENIRGAARLAKAHGKAFYIDGCRFAENAWFIKLREEGQKDRSIKEIV
RETFALADGMTMSAKKDAFANIGGWLALNNDALAERARTLLIQTEGFPTYGGLAGRDLDAIAQGLKEIIDEDYLRYRVRT
NAYIAERLDAMGVPVVKPAGGHAVFIDARAFLPHIPPLEYPGQALTCAMYETGGIRACEIGTVMFGRKPDGTEAPGAMDL
VRLAMPRRVYTQSHADYVVEVLEDVAATKDTLKGLRIVKEPPMMRHFTAAFERL
>Mature_454_residues
MKTIIEPFRIKSVEPIRMTTREERARLLEAAGFNLFKLHSDDVIIDLLTDSGTSAMSAAQWGAVMTGDESYAGAPSFYRF
EAAVKDLMDFTHIIPAHQGRAAEHLLFSLIAKTGDLIPSNTHFDTTRGNIEAMGAEALDLPIAEGRVPSLDHPFKGNMDL
AALERVLTEEGGRIPAVMMTITNNAGGGQPVSLENIRGAARLAKAHGKAFYIDGCRFAENAWFIKLREEGQKDRSIKEIV
RETFALADGMTMSAKKDAFANIGGWLALNNDALAERARTLLIQTEGFPTYGGLAGRDLDAIAQGLKEIIDEDYLRYRVRT
NAYIAERLDAMGVPVVKPAGGHAVFIDARAFLPHIPPLEYPGQALTCAMYETGGIRACEIGTVMFGRKPDGTEAPGAMDL
VRLAMPRRVYTQSHADYVVEVLEDVAATKDTLKGLRIVKEPPMMRHFTAAFERL

Specific function: Tryptophan catabolism. [C]

COG id: COG3033

COG function: function code E; Tryptophanase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-eliminating lyase family

Homologues:

Organism=Escherichia coli, GI87082323, Length=469, Percent_Identity=41.5778251599147, Blast_Score=378, Evalue=1e-106,

Paralogues:

None

Copy number: 7160 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 460 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TNAA_HYPNA (Q0C406)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_759537.1
- ProteinModelPortal:   Q0C406
- SMR:   Q0C406
- STRING:   Q0C406
- GeneID:   4287932
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_0810
- NMPDR:   fig|228405.5.peg.792
- TIGR:   HNE_0810
- eggNOG:   COG3033
- HOGENOM:   HBG297784
- OMA:   DTTRAHV
- PhylomeDB:   Q0C406
- ProtClustDB:   PRK13238
- BioCyc:   HNEP81032:HNE_0810-MONOMER
- HAMAP:   MF_00544
- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR011166
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PIRSF:   PIRSF001386

Pfam domain/function: PF01212 Beta_elim_lyase; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =4.1.99.1

Molecular weight: Translated: 49741; Mature: 49741

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00853 BETA_ELIM_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIIEPFRIKSVEPIRMTTREERARLLEAAGFNLFKLHSDDVIIDLLTDSGTSAMSAAQ
CCCCCCCHHCCCCCCHHHCCHHHHHHHHHHCCCCEEEECCCCEEEEEEECCCCCHHHHHH
WGAVMTGDESYAGAPSFYRFEAAVKDLMDFTHIIPAHQGRAAEHLLFSLIAKTGDLIPSN
CCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCC
THFDTTRGNIEAMGAEALDLPIAEGRVPSLDHPFKGNMDLAALERVLTEEGGRIPAVMMT
CCCCCCCCCEEECCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEE
ITNNAGGGQPVSLENIRGAARLAKAHGKAFYIDGCRFAENAWFIKLREEGQKDRSIKEIV
EECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECEEECCCEEEEEECCCCCCCHHHHHHH
RETFALADGMTMSAKKDAFANIGGWLALNNDALAERARTLLIQTEGFPTYGGLAGRDLDA
HHHHHHHCCCCCCCCHHHHHCCCCEEEECCHHHHHHHHEEEEEECCCCCCCCCCCCCHHH
IAQGLKEIIDEDYLRYRVRTNAYIAERLDAMGVPVVKPAGGHAVFIDARAFLPHIPPLEY
HHHHHHHHHHHHHHEEEECCCHHHHHHHHHCCCCEECCCCCCEEEEECHHHCCCCCCCCC
PGQALTCAMYETGGIRACEIGTVMFGRKPDGTEAPGAMDLVRLAMPRRVYTQSHADYVVE
CCCEEEEEEEECCCEEEEEECEEEECCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHH
VLEDVAATKDTLKGLRIVKEPPMMRHFTAAFERL
HHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKTIIEPFRIKSVEPIRMTTREERARLLEAAGFNLFKLHSDDVIIDLLTDSGTSAMSAAQ
CCCCCCCHHCCCCCCHHHCCHHHHHHHHHHCCCCEEEECCCCEEEEEEECCCCCHHHHHH
WGAVMTGDESYAGAPSFYRFEAAVKDLMDFTHIIPAHQGRAAEHLLFSLIAKTGDLIPSN
CCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCC
THFDTTRGNIEAMGAEALDLPIAEGRVPSLDHPFKGNMDLAALERVLTEEGGRIPAVMMT
CCCCCCCCCEEECCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEE
ITNNAGGGQPVSLENIRGAARLAKAHGKAFYIDGCRFAENAWFIKLREEGQKDRSIKEIV
EECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECEEECCCEEEEEECCCCCCCHHHHHHH
RETFALADGMTMSAKKDAFANIGGWLALNNDALAERARTLLIQTEGFPTYGGLAGRDLDA
HHHHHHHCCCCCCCCHHHHHCCCCEEEECCHHHHHHHHEEEEEECCCCCCCCCCCCCHHH
IAQGLKEIIDEDYLRYRVRTNAYIAERLDAMGVPVVKPAGGHAVFIDARAFLPHIPPLEY
HHHHHHHHHHHHHHEEEECCCHHHHHHHHHCCCCEECCCCCCEEEEECHHHCCCCCCCCC
PGQALTCAMYETGGIRACEIGTVMFGRKPDGTEAPGAMDLVRLAMPRRVYTQSHADYVVE
CCCEEEEEEEECCCEEEEEECEEEECCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHH
VLEDVAATKDTLKGLRIVKEPPMMRHFTAAFERL
HHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA