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The map label for this gene is argF

Identifier: 114799893

GI number: 114799893

Start: 588075

End: 589001

Strand: Direct

Name: argF

Synonym: HNE_0592

Alternate gene names: 114799893

Gene position: 588075-589001 (Clockwise)

Preceding gene: 114798816

Following gene: 114798903

Centisome position: 15.87

GC content: 63.54

Gene sequence:

>927_bases
ATGACGATCCGCCACTTCCTCGACATCTGGCCGCTTGAGAGCGCAGAGCTTCGTGCGATCCTCGATCAGGCACACGCCAT
GAAGAAGGCCCGTGCCGGCTGGCCGAAGGGCAAGATTGATGCGGGCGCGCCGCTCGATGGCCACACGCTGGCGATGATTT
TCGAGAAATCCTCCACGCGCACCCGCTTCTCCTTCGACATGGCAATGCGCCAGCTCGGCGGCTCGTCGATCACGGCGACA
TCCAACGACATGCAGCTTGGCCGCGGTGAAACCGTGGAAGATACCGCCAAGGTGCTCTCGCGCTTTGTCGATGCCGTGAT
GATCCGCGCCAATGACCATGCCGATGTCGAGGCATTTGCCGACGCCGCCAGCGTCCCGGTCATCAACGGCCTCACGGACC
GCTCCCACCCCTGCCAGATCATGGCAGACCTGATGACGCTGGAAGAACACGGCCTGACGCTCCGGGGCGCGCGCCTTGCC
TGGGTTGGCGATGGCAATAATGTCTGCGCCAGCTTCATCCATGCCGCGCCCAAATTCGGCTTCTCGCTGGCGGTCGGCAC
ACCCAAACGCTTTGCTCCGGATGAGGACGATCTGGAAATCGCCGCCGAGCTTCAGGGCCGGATCGACCTTTATGAGACCG
CCGAGGAAGCCGTCGCCGGCGCCGATGTCGTCATCGCGGACACCTTTGTGTCGATGGGCGACGTGGACGCCGAACGCCGC
CTCGAAATCCTCGACCCCTACGCGGTCACCGAGGAGCTGATGGAACTGGCCGCTGGCGGGGCAAAATTCCTGCACTGCCT
CCCGGCTCACCGGGGCGAGGAAGTCGATGCCGAAGTGATCGACGGTCCGGCCAGCCTCGTCTTCGACGAAGCGGAAAACC
GCCTCCACGCCCAGAAAGCCATCCTGCGCTGGTGTCTCGGTAAATAA

Upstream 100 bases:

>100_bases
CCGGGCGGGCAGTTCTTCCCGCACCGCCTTAACCAACAAACACTGGCGATCCCCCCAAAAACGCGCTATGCGGCGCCCTT
TCTTTTGCGGGGCCGCGCCC

Downstream 100 bases:

>100_bases
ATTTGGGACGTAAGCCCGGCCATCACTTTCCCGCGCGCCCCGCTTGGCGGGCAGGGGCACGCGCCTTAGGGTTCTCTCAT
GGCTGACACATTCCACCCGC

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MTIRHFLDIWPLESAELRAILDQAHAMKKARAGWPKGKIDAGAPLDGHTLAMIFEKSSTRTRFSFDMAMRQLGGSSITAT
SNDMQLGRGETVEDTAKVLSRFVDAVMIRANDHADVEAFADAASVPVINGLTDRSHPCQIMADLMTLEEHGLTLRGARLA
WVGDGNNVCASFIHAAPKFGFSLAVGTPKRFAPDEDDLEIAAELQGRIDLYETAEEAVAGADVVIADTFVSMGDVDAERR
LEILDPYAVTEELMELAAGGAKFLHCLPAHRGEEVDAEVIDGPASLVFDEAENRLHAQKAILRWCLGK

Sequences:

>Translated_308_residues
MTIRHFLDIWPLESAELRAILDQAHAMKKARAGWPKGKIDAGAPLDGHTLAMIFEKSSTRTRFSFDMAMRQLGGSSITAT
SNDMQLGRGETVEDTAKVLSRFVDAVMIRANDHADVEAFADAASVPVINGLTDRSHPCQIMADLMTLEEHGLTLRGARLA
WVGDGNNVCASFIHAAPKFGFSLAVGTPKRFAPDEDDLEIAAELQGRIDLYETAEEAVAGADVVIADTFVSMGDVDAERR
LEILDPYAVTEELMELAAGGAKFLHCLPAHRGEEVDAEVIDGPASLVFDEAENRLHAQKAILRWCLGK
>Mature_307_residues
TIRHFLDIWPLESAELRAILDQAHAMKKARAGWPKGKIDAGAPLDGHTLAMIFEKSSTRTRFSFDMAMRQLGGSSITATS
NDMQLGRGETVEDTAKVLSRFVDAVMIRANDHADVEAFADAASVPVINGLTDRSHPCQIMADLMTLEEHGLTLRGARLAW
VGDGNNVCASFIHAAPKFGFSLAVGTPKRFAPDEDDLEIAAELQGRIDLYETAEEAVAGADVVIADTFVSMGDVDAERRL
EILDPYAVTEELMELAAGGAKFLHCLPAHRGEEVDAEVIDGPASLVFDEAENRLHAQKAILRWCLGK

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=255, Percent_Identity=44.3137254901961, Blast_Score=214, Evalue=5e-56,
Organism=Homo sapiens, GI18105007, Length=318, Percent_Identity=26.1006289308176, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1790703, Length=333, Percent_Identity=36.6366366366366, Blast_Score=184, Evalue=6e-48,
Organism=Escherichia coli, GI1786469, Length=333, Percent_Identity=35.4354354354354, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI2367364, Length=312, Percent_Identity=28.525641025641, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI48994908, Length=320, Percent_Identity=27.5, Blast_Score=80, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI193204318, Length=300, Percent_Identity=25.6666666666667, Blast_Score=81, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6322373, Length=311, Percent_Identity=35.3697749196142, Blast_Score=189, Evalue=6e-49,
Organism=Saccharomyces cerevisiae, GI6322331, Length=313, Percent_Identity=25.5591054313099, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24642586, Length=271, Percent_Identity=27.3062730627306, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTC_HYPNA (Q0C4M2)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_759321.1
- ProteinModelPortal:   Q0C4M2
- SMR:   Q0C4M2
- STRING:   Q0C4M2
- GeneID:   4290222
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_0592
- NMPDR:   fig|228405.5.peg.580
- TIGR:   HNE_0592
- eggNOG:   COG0078
- HOGENOM:   HBG579429
- OMA:   AKFLHCL
- PhylomeDB:   Q0C4M2
- ProtClustDB:   PRK00779
- BioCyc:   HNEP81032:HNE_0592-MONOMER
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 33442; Mature: 33311

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 109-109 BINDING 136-136

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIRHFLDIWPLESAELRAILDQAHAMKKARAGWPKGKIDAGAPLDGHTLAMIFEKSSTR
CCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCC
TRFSFDMAMRQLGGSSITATSNDMQLGRGETVEDTAKVLSRFVDAVMIRANDHADVEAFA
EEHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCCHHHHH
DAASVPVINGLTDRSHPCQIMADLMTLEEHGLTLRGARLAWVGDGNNVCASFIHAAPKFG
CCCCCCEECCCCCCCCHHHHHHHHHHHHHCCCEEECCEEEEEECCCHHHHHHHHHCCCCC
FSLAVGTPKRFAPDEDDLEIAAELQGRIDLYETAEEAVAGADVVIADTFVSMGDVDAERR
EEEEECCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCEEEEHHHHHCCCCCHHHH
LEILDPYAVTEELMELAAGGAKFLHCLPAHRGEEVDAEVIDGPASLVFDEAENRLHAQKA
HHHCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHH
ILRWCLGK
HHHHHCCC
>Mature Secondary Structure 
TIRHFLDIWPLESAELRAILDQAHAMKKARAGWPKGKIDAGAPLDGHTLAMIFEKSSTR
CHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCC
TRFSFDMAMRQLGGSSITATSNDMQLGRGETVEDTAKVLSRFVDAVMIRANDHADVEAFA
EEHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCCHHHHH
DAASVPVINGLTDRSHPCQIMADLMTLEEHGLTLRGARLAWVGDGNNVCASFIHAAPKFG
CCCCCCEECCCCCCCCHHHHHHHHHHHHHCCCEEECCEEEEEECCCHHHHHHHHHCCCCC
FSLAVGTPKRFAPDEDDLEIAAELQGRIDLYETAEEAVAGADVVIADTFVSMGDVDAERR
EEEEECCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCEEEEHHHHHCCCCCHHHH
LEILDPYAVTEELMELAAGGAKFLHCLPAHRGEEVDAEVIDGPASLVFDEAENRLHAQKA
HHHCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHH
ILRWCLGK
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA