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The map label for this gene is yjeA [H]

Identifier: 114799660

GI number: 114799660

Start: 3318452

End: 3319495

Strand: Reverse

Name: yjeA [H]

Synonym: HNE_3139

Alternate gene names: 114799660

Gene position: 3319495-3318452 (Counterclockwise)

Preceding gene: 114798578

Following gene: 114797088

Centisome position: 89.59

GC content: 60.82

Gene sequence:

>1044_bases
ATGGACGTATCTGGATGGTGGAATCCTGAGGTACATGCCCGCCGCCGCGGTCACTTGCTGGCACGCGGACGGGTGAAGAC
GGCTTTGCGCCGCTGGTTTGATGGCGAAGGGTTTGTCGAGACCGAATGCGGCGCACTCGTCGTTTCCCCCGGAAATGAAG
CGCATCTGCATGCTTTCGAGACCCAGTTTGTTGCCGAAAATGGACAAACCCGGTCATTTTACCTCCATACCTCGCCAGAA
TTTGCCATGAAGAAGCTTCTGGCGGCTGGGGAGCCGCGCATTTTCGATTTCGCCAGAGCGTATCGCAACCGGGAGACCGG
CGACCGCCACGCGCCTGAATTCACGATGCTCGAATGGTACAGGGCCGGCGCAGAGATGGCCGATGTCATGGGCGACTGCG
TATCCCTTGCGCGCATTGCGGCTGAAGCGGCCGGAAAGGGGCAGCTGGTCTGGAAAGGCCACAGCTGTGATCCTGGATTG
GAGCCGGAAACGCTTACGCTGGTAGACGCGTTCGAGCGATATGCCCGCATCGATCTGGCGCGGCATCTGGAAGATATAGA
AGGGTTTCACGCTGCCGCTCTGGCTGCCGGCGTGGGGATCTCCGCGGGGGCGAGTTGGACCGATATTTTCTCGGCGGTTC
TCGTCACGAAAATTGAGCCGCATCTCGGAATGGAGCGGCTGACCTTCCTGTGCCGCTATCCGATCAGCGAAGCGGCTCTT
TCGCGGCCGGCACCTGATGATCCACGCTTTGCCGAACGTTTTGAGATGTATGCCTGCGGTGTCGAGCTTGCCAATGGCTA
CCGGGAACTGACGGACGCTGTCCTGCTTGAAGCGCGTCAGCGCGCCGAGATGCATATCAAGGAGGCGGTCTATGGGGAAC
GGTATCCTCTTGACCCGGACTTCATCGATGCCGTCGCCGTTATGCCGGAAGCGAGCGGGGTGGCGCTTGGCTTTGACCGG
TTGGCCATGCTGGCAAGCGGCGCGCCGTCCATCTCGGATGTCCTCTGGACGCCGCCCGTGATCCTAGAGGAGCCCGCGTC
ATGA

Upstream 100 bases:

>100_bases
GCTATATACCGCCTCCGCACCATTTGGCCAGCCGCATGCTGTGGCTTTTCAGTCTGGCGCGGCGGGCCGCGACTGGCCTT
GAACCCCTGGAATTGGCTTT

Downstream 100 bases:

>100_bases
AGCCGGTGACATATCGGCATGCCGGGGAGTTGCTGACTGCGGGGATCATATCGGCAGATCAGCTGCCGGTCGTTTCCCGC
GTCGCGGAAAACTATGTGAT

Product: tRNA synthetase, class II

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MDVSGWWNPEVHARRRGHLLARGRVKTALRRWFDGEGFVETECGALVVSPGNEAHLHAFETQFVAENGQTRSFYLHTSPE
FAMKKLLAAGEPRIFDFARAYRNRETGDRHAPEFTMLEWYRAGAEMADVMGDCVSLARIAAEAAGKGQLVWKGHSCDPGL
EPETLTLVDAFERYARIDLARHLEDIEGFHAAALAAGVGISAGASWTDIFSAVLVTKIEPHLGMERLTFLCRYPISEAAL
SRPAPDDPRFAERFEMYACGVELANGYRELTDAVLLEARQRAEMHIKEAVYGERYPLDPDFIDAVAVMPEASGVALGFDR
LAMLASGAPSISDVLWTPPVILEEPAS

Sequences:

>Translated_347_residues
MDVSGWWNPEVHARRRGHLLARGRVKTALRRWFDGEGFVETECGALVVSPGNEAHLHAFETQFVAENGQTRSFYLHTSPE
FAMKKLLAAGEPRIFDFARAYRNRETGDRHAPEFTMLEWYRAGAEMADVMGDCVSLARIAAEAAGKGQLVWKGHSCDPGL
EPETLTLVDAFERYARIDLARHLEDIEGFHAAALAAGVGISAGASWTDIFSAVLVTKIEPHLGMERLTFLCRYPISEAAL
SRPAPDDPRFAERFEMYACGVELANGYRELTDAVLLEARQRAEMHIKEAVYGERYPLDPDFIDAVAVMPEASGVALGFDR
LAMLASGAPSISDVLWTPPVILEEPAS
>Mature_347_residues
MDVSGWWNPEVHARRRGHLLARGRVKTALRRWFDGEGFVETECGALVVSPGNEAHLHAFETQFVAENGQTRSFYLHTSPE
FAMKKLLAAGEPRIFDFARAYRNRETGDRHAPEFTMLEWYRAGAEMADVMGDCVSLARIAAEAAGKGQLVWKGHSCDPGL
EPETLTLVDAFERYARIDLARHLEDIEGFHAAALAAGVGISAGASWTDIFSAVLVTKIEPHLGMERLTFLCRYPISEAAL
SRPAPDDPRFAERFEMYACGVELANGYRELTDAVLLEARQRAEMHIKEAVYGERYPLDPDFIDAVAVMPEASGVALGFDR
LAMLASGAPSISDVLWTPPVILEEPAS

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI87082379, Length=343, Percent_Identity=35.2769679300292, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1790571, Length=335, Percent_Identity=32.5373134328358, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1789256, Length=333, Percent_Identity=31.5315315315315, Blast_Score=145, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6320242, Length=353, Percent_Identity=26.3456090651558, Blast_Score=107, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6

Molecular weight: Translated: 38338; Mature: 38338

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVSGWWNPEVHARRRGHLLARGRVKTALRRWFDGEGFVETECGALVVSPGNEAHLHAFE
CCCCCCCCCCHHHHHCCCEEEHHHHHHHHHHHCCCCCCEEECCCEEEECCCCCCEEEHHH
TQFVAENGQTRSFYLHTSPEFAMKKLLAAGEPRIFDFARAYRNRETGDRHAPEFTMLEWY
HHHHHCCCCCEEEEEECCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHH
RAGAEMADVMGDCVSLARIAAEAAGKGQLVWKGHSCDPGLEPETLTLVDAFERYARIDLA
HHCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
RHLEDIEGFHAAALAAGVGISAGASWTDIFSAVLVTKIEPHLGMERLTFLCRYPISEAAL
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHH
SRPAPDDPRFAERFEMYACGVELANGYRELTDAVLLEARQRAEMHIKEAVYGERYPLDPD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
FIDAVAVMPEASGVALGFDRLAMLASGAPSISDVLWTPPVILEEPAS
HHHHHHHCCCCCCCEECHHHHHHHHCCCCCHHHHHCCCCEEECCCCC
>Mature Secondary Structure
MDVSGWWNPEVHARRRGHLLARGRVKTALRRWFDGEGFVETECGALVVSPGNEAHLHAFE
CCCCCCCCCCHHHHHCCCEEEHHHHHHHHHHHCCCCCCEEECCCEEEECCCCCCEEEHHH
TQFVAENGQTRSFYLHTSPEFAMKKLLAAGEPRIFDFARAYRNRETGDRHAPEFTMLEWY
HHHHHCCCCCEEEEEECCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHH
RAGAEMADVMGDCVSLARIAAEAAGKGQLVWKGHSCDPGLEPETLTLVDAFERYARIDLA
HHCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
RHLEDIEGFHAAALAAGVGISAGASWTDIFSAVLVTKIEPHLGMERLTFLCRYPISEAAL
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHH
SRPAPDDPRFAERFEMYACGVELANGYRELTDAVLLEARQRAEMHIKEAVYGERYPLDPD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
FIDAVAVMPEASGVALGFDRLAMLASGAPSISDVLWTPPVILEEPAS
HHHHHHHCCCCCCCEECHHHHHHHHCCCCCHHHHHCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA