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The map label for this gene is lcfB [H]

Identifier: 114799617

GI number: 114799617

Start: 2406268

End: 2407815

Strand: Direct

Name: lcfB [H]

Synonym: HNE_2308

Alternate gene names: 114799617

Gene position: 2406268-2407815 (Clockwise)

Preceding gene: 114798019

Following gene: 114799177

Centisome position: 64.95

GC content: 62.34

Gene sequence:

>1548_bases
ATGCACCCCAGTCACCACGCGCGGACCAGCCCTGACAGACCCGCCATCATCATGGCCGGCTCGGGCGAAACGATCAGCTT
CCGCCAGCTCGACGAGCGCTCCAACCAGATCGCCCACGCCCTGCGCGCCGCGGGCTGCCAGCCGGGCGACACCATCGCCA
TCTTTGCGGAAAATTCCCCGCGCTATTTCGAAATCTGCTGGGGCGCCCAGCGCGCCGGCCTTTACTATGTGTGCATTTCC
TCGCGCCTCACCGCGCCGGAAGTGAAATACATCATCGAGGATTCCGGCTCGAAACTGCTCATCACCGGTGCCAACAAAGG
CGCGGTTGCCAAAGAAGCCGCCGCCGCCGCAGGCACGAAAGACCTCTGGTCAATTGACGGCGAAGTCGAAGGTTTCAAGC
CGCTTGAGGCCCTCGCCGCCCCCTTCCCAACAACGCCCATCGCAGACGAGATGGCCGGCACGGACATGCTCTACTCCTCG
GGCACAACGGGCCGCCCGAAAGGCATCCGCCCGCCGCTGGAACGGGGCCTCCCGATCGACGCCGACAACATCCTCGTCCA
GATCGCCCGCGCCATGTCCGGCGCCAGCCCGGAGTCGGTCTACCTCTCCCCGGCCCCGCTTTACCACGCCGCGCCCCTGC
GCTGGTGCATGACGTTCACCCGCATCGGGTCCACCGTCATCGTGATGGAAAAGTTCGATCCCGAAGAATTCCTGAAAGCA
GTCGAGAAATACAAGGTCACCCACACGCAGGTCGTGCCGACCATGTTCGTGAAAATGCTGAAACTGCCCGAAGATGTCCG
CATGAAATACGACGTCTCCAGCATGACCTTCGCCATCCACGCCGCCGCGCCCTGCCCGGTGCCGGTCAAGGAACAGATGA
TCGCCTGGTGGGGCCCGGTCATCGACGAATACTATGCCGGCTCTGAAGGCAACGGCATGACCTATGTGAAAAGCCCCGAC
TGGCTGACCCACAAGGGCACCGTCGGCCGCCCGATCCACGGCCAGGTCCACATCTGCGATGAAGAAGGCAACGAGCTGCC
CATCGGCGAAGAAGGCCAGGTCTATTTCTCCGGCACCGTTCCGCCGAACTATCACAACGACCCGGAAAAGAACAAAGCCG
CCCTCAACCCGATCCACCCCGACTGGTCGTCCCTCGGCGATGTCGGCAAGCTGGACGCCGACGGCTTCCTCTATCTGACG
GACCGCAAGTCCTTCATGATCATCTCCGGCGGCGTGAACATCTACCCGCAGGAAACCGAAAACGTTCTCATCACCCACCC
CAAAGTGGCTGATGTCGCCGTTATCGGCGTGCCCGATGAAGATTTCGGCGAAGCGGTGAAAGCCGTTGTCCAGCCCATGC
CCGGCATCGCGCATTCCGAAGAACTCGCCGCCGAGCTGATGGCCTTCTGCCAGGCAAACCTCTCCAAACTCAAATGCCCC
AAAAGCATCGACTTTGACCCTGAGCTGCCCCGCCACGCCACGGGCAAACTCTACAAACGCCTCATCCGCGACCGCTACTG
GGGCAACAAGAACAGCCGCATCGTCTGA

Upstream 100 bases:

>100_bases
TTAGGTCAGTCGTGGACAGGCCCTTAAGGCTCGTTAAAGTCCGCCGCGAGACGGCAGCCAGCGCCTTGCGCGAGGCTTCA
CACCCGGAGGATAAGACGCC

Downstream 100 bases:

>100_bases
TCGCGGGCCGGCAAGTCTGAAACGCGGCGCGGGATGGCAAGGGGCTGTCCCGCGCCGTTTCCGTTCCGGGTGCCCGCGCA
CCCCCAAACGAAAAACACCG

Product: AMP-binding protein

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MHPSHHARTSPDRPAIIMAGSGETISFRQLDERSNQIAHALRAAGCQPGDTIAIFAENSPRYFEICWGAQRAGLYYVCIS
SRLTAPEVKYIIEDSGSKLLITGANKGAVAKEAAAAAGTKDLWSIDGEVEGFKPLEALAAPFPTTPIADEMAGTDMLYSS
GTTGRPKGIRPPLERGLPIDADNILVQIARAMSGASPESVYLSPAPLYHAAPLRWCMTFTRIGSTVIVMEKFDPEEFLKA
VEKYKVTHTQVVPTMFVKMLKLPEDVRMKYDVSSMTFAIHAAAPCPVPVKEQMIAWWGPVIDEYYAGSEGNGMTYVKSPD
WLTHKGTVGRPIHGQVHICDEEGNELPIGEEGQVYFSGTVPPNYHNDPEKNKAALNPIHPDWSSLGDVGKLDADGFLYLT
DRKSFMIISGGVNIYPQETENVLITHPKVADVAVIGVPDEDFGEAVKAVVQPMPGIAHSEELAAELMAFCQANLSKLKCP
KSIDFDPELPRHATGKLYKRLIRDRYWGNKNSRIV

Sequences:

>Translated_515_residues
MHPSHHARTSPDRPAIIMAGSGETISFRQLDERSNQIAHALRAAGCQPGDTIAIFAENSPRYFEICWGAQRAGLYYVCIS
SRLTAPEVKYIIEDSGSKLLITGANKGAVAKEAAAAAGTKDLWSIDGEVEGFKPLEALAAPFPTTPIADEMAGTDMLYSS
GTTGRPKGIRPPLERGLPIDADNILVQIARAMSGASPESVYLSPAPLYHAAPLRWCMTFTRIGSTVIVMEKFDPEEFLKA
VEKYKVTHTQVVPTMFVKMLKLPEDVRMKYDVSSMTFAIHAAAPCPVPVKEQMIAWWGPVIDEYYAGSEGNGMTYVKSPD
WLTHKGTVGRPIHGQVHICDEEGNELPIGEEGQVYFSGTVPPNYHNDPEKNKAALNPIHPDWSSLGDVGKLDADGFLYLT
DRKSFMIISGGVNIYPQETENVLITHPKVADVAVIGVPDEDFGEAVKAVVQPMPGIAHSEELAAELMAFCQANLSKLKCP
KSIDFDPELPRHATGKLYKRLIRDRYWGNKNSRIV
>Mature_515_residues
MHPSHHARTSPDRPAIIMAGSGETISFRQLDERSNQIAHALRAAGCQPGDTIAIFAENSPRYFEICWGAQRAGLYYVCIS
SRLTAPEVKYIIEDSGSKLLITGANKGAVAKEAAAAAGTKDLWSIDGEVEGFKPLEALAAPFPTTPIADEMAGTDMLYSS
GTTGRPKGIRPPLERGLPIDADNILVQIARAMSGASPESVYLSPAPLYHAAPLRWCMTFTRIGSTVIVMEKFDPEEFLKA
VEKYKVTHTQVVPTMFVKMLKLPEDVRMKYDVSSMTFAIHAAAPCPVPVKEQMIAWWGPVIDEYYAGSEGNGMTYVKSPD
WLTHKGTVGRPIHGQVHICDEEGNELPIGEEGQVYFSGTVPPNYHNDPEKNKAALNPIHPDWSSLGDVGKLDADGFLYLT
DRKSFMIISGGVNIYPQETENVLITHPKVADVAVIGVPDEDFGEAVKAVVQPMPGIAHSEELAAELMAFCQANLSKLKCP
KSIDFDPELPRHATGKLYKRLIRDRYWGNKNSRIV

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=555, Percent_Identity=26.8468468468468, Blast_Score=166, Evalue=7e-41,
Organism=Homo sapiens, GI157311624, Length=533, Percent_Identity=27.3921200750469, Blast_Score=139, Evalue=9e-33,
Organism=Homo sapiens, GI157311622, Length=533, Percent_Identity=27.3921200750469, Blast_Score=139, Evalue=9e-33,
Organism=Homo sapiens, GI58082049, Length=523, Percent_Identity=27.5334608030593, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI38505220, Length=527, Percent_Identity=27.3244781783681, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI122937307, Length=538, Percent_Identity=27.3234200743494, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI115511026, Length=514, Percent_Identity=26.2645914396887, Blast_Score=119, Evalue=8e-27,
Organism=Homo sapiens, GI42544132, Length=535, Percent_Identity=26.3551401869159, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI187761345, Length=533, Percent_Identity=26.266416510319, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI187761343, Length=533, Percent_Identity=26.266416510319, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI38524616, Length=470, Percent_Identity=26.5957446808511, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI227499619, Length=486, Percent_Identity=24.0740740740741, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI40807357, Length=549, Percent_Identity=25.1366120218579, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI62865631, Length=539, Percent_Identity=21.5213358070501, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI13325055, Length=539, Percent_Identity=21.5213358070501, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI13375727, Length=545, Percent_Identity=21.651376146789, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI8923896, Length=378, Percent_Identity=23.015873015873, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI253970489, Length=378, Percent_Identity=23.015873015873, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI195947378, Length=411, Percent_Identity=23.3576642335766, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI1788107, Length=544, Percent_Identity=28.3088235294118, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI221142682, Length=519, Percent_Identity=26.3969171483622, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI145693145, Length=516, Percent_Identity=24.2248062015504, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1790505, Length=554, Percent_Identity=22.9241877256318, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1786529, Length=539, Percent_Identity=24.860853432282, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1786810, Length=501, Percent_Identity=22.7544910179641, Blast_Score=89, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17531443, Length=522, Percent_Identity=28.544061302682, Blast_Score=171, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI17558820, Length=511, Percent_Identity=27.9843444227006, Blast_Score=151, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI71994703, Length=539, Percent_Identity=26.1595547309833, Blast_Score=144, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI71994690, Length=539, Percent_Identity=26.1595547309833, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI71994694, Length=539, Percent_Identity=26.1595547309833, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17559526, Length=506, Percent_Identity=27.6679841897233, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32563687, Length=508, Percent_Identity=25.9842519685039, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17557194, Length=538, Percent_Identity=26.7657992565056, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17538037, Length=526, Percent_Identity=26.2357414448669, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17560308, Length=543, Percent_Identity=23.7569060773481, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17560140, Length=510, Percent_Identity=22.156862745098, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17551278, Length=560, Percent_Identity=24.4642857142857, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI115534440, Length=552, Percent_Identity=22.463768115942, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71983001, Length=541, Percent_Identity=22.365988909427, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71982997, Length=541, Percent_Identity=22.365988909427, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=365, Percent_Identity=30.6849315068493, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6323182, Length=581, Percent_Identity=23.407917383821, Blast_Score=101, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21355181, Length=521, Percent_Identity=26.4875239923225, Blast_Score=145, Evalue=9e-35,
Organism=Drosophila melanogaster, GI24581924, Length=543, Percent_Identity=24.4935543278085, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI18859661, Length=515, Percent_Identity=24.2718446601942, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI21356947, Length=521, Percent_Identity=25.5278310940499, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24648260, Length=504, Percent_Identity=26.3888888888889, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24653035, Length=530, Percent_Identity=24.1509433962264, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI161076582, Length=542, Percent_Identity=23.9852398523985, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24656500, Length=522, Percent_Identity=22.7969348659004, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24648253, Length=519, Percent_Identity=23.121387283237, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI62472339, Length=551, Percent_Identity=22.3230490018149, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648255, Length=519, Percent_Identity=22.7360308285164, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19922652, Length=448, Percent_Identity=24.1071428571429, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24667955, Length=543, Percent_Identity=22.4677716390424, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI20130357, Length=408, Percent_Identity=25.7352941176471, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21356441, Length=454, Percent_Identity=22.6872246696035, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24667959, Length=382, Percent_Identity=24.0837696335079, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI21358303, Length=455, Percent_Identity=21.0989010989011, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI281365686, Length=310, Percent_Identity=23.8709677419355, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21355225, Length=555, Percent_Identity=20.7207207207207, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI281364798, Length=483, Percent_Identity=24.4306418219462, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24583465, Length=483, Percent_Identity=24.4306418219462, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24583463, Length=483, Percent_Identity=24.4306418219462, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24648257, Length=511, Percent_Identity=22.7005870841487, Blast_Score=82, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 56392; Mature: 56392

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPSHHARTSPDRPAIIMAGSGETISFRQLDERSNQIAHALRAAGCQPGDTIAIFAENSP
CCCCCCCCCCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCC
RYFEICWGAQRAGLYYVCISSRLTAPEVKYIIEDSGSKLLITGANKGAVAKEAAAAAGTK
CEEEEEECCCCCCEEEEEECCCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHCCCC
DLWSIDGEVEGFKPLEALAAPFPTTPIADEMAGTDMLYSSGTTGRPKGIRPPLERGLPID
HHEECCCCCCCCCHHHHHHCCCCCCCCHHHHCCCCEEECCCCCCCCCCCCCHHHCCCCCC
ADNILVQIARAMSGASPESVYLSPAPLYHAAPLRWCMTFTRIGSTVIVMEKFDPEEFLKA
HHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHH
VEKYKVTHTQVVPTMFVKMLKLPEDVRMKYDVSSMTFAIHAAAPCPVPVKEQMIAWWGPV
HHHHCCHHHHHHHHHHHHHHHCCHHHEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHH
IDEYYAGSEGNGMTYVKSPDWLTHKGTVGRPIHGQVHICDEEGNELPIGEEGQVYFSGTV
HHHHHCCCCCCCEEEEECCCCEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCC
PPNYHNDPEKNKAALNPIHPDWSSLGDVGKLDADGFLYLTDRKSFMIISGGVNIYPQETE
CCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCEEEEECCCEEEEEECCCEECCCCCC
NVLITHPKVADVAVIGVPDEDFGEAVKAVVQPMPGIAHSEELAAELMAFCQANLSKLKCP
CEEEECCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
KSIDFDPELPRHATGKLYKRLIRDRYWGNKNSRIV
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MHPSHHARTSPDRPAIIMAGSGETISFRQLDERSNQIAHALRAAGCQPGDTIAIFAENSP
CCCCCCCCCCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCC
RYFEICWGAQRAGLYYVCISSRLTAPEVKYIIEDSGSKLLITGANKGAVAKEAAAAAGTK
CEEEEEECCCCCCEEEEEECCCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHCCCC
DLWSIDGEVEGFKPLEALAAPFPTTPIADEMAGTDMLYSSGTTGRPKGIRPPLERGLPID
HHEECCCCCCCCCHHHHHHCCCCCCCCHHHHCCCCEEECCCCCCCCCCCCCHHHCCCCCC
ADNILVQIARAMSGASPESVYLSPAPLYHAAPLRWCMTFTRIGSTVIVMEKFDPEEFLKA
HHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHH
VEKYKVTHTQVVPTMFVKMLKLPEDVRMKYDVSSMTFAIHAAAPCPVPVKEQMIAWWGPV
HHHHCCHHHHHHHHHHHHHHHCCHHHEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHH
IDEYYAGSEGNGMTYVKSPDWLTHKGTVGRPIHGQVHICDEEGNELPIGEEGQVYFSGTV
HHHHHCCCCCCCEEEEECCCCEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCC
PPNYHNDPEKNKAALNPIHPDWSSLGDVGKLDADGFLYLTDRKSFMIISGGVNIYPQETE
CCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCEEEEECCCEEEEEECCCEECCCCCC
NVLITHPKVADVAVIGVPDEDFGEAVKAVVQPMPGIAHSEELAAELMAFCQANLSKLKCP
CEEEECCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
KSIDFDPELPRHATGKLYKRLIRDRYWGNKNSRIV
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]