Click here to switch to the map view.

The map label for this gene is 114799306

Identifier: 114799306

GI number: 114799306

Start: 3158427

End: 3159269

Strand: Reverse

Name: 114799306

Synonym: HNE_2997

Alternate gene names: NA

Gene position: 3159269-3158427 (Counterclockwise)

Preceding gene: 114798798

Following gene: 114798864

Centisome position: 85.27

GC content: 61.21

Gene sequence:

>843_bases
ATGACCCGGACGATTACGCTCGCAGCCATCCAGTTTACCCCCACCGACGATGTTCAGGAGAACATCGACCGGGTTGCCGG
TTTCGTGCGCGAGGCGGCGGCCAAGGGCGCAGATGTCATTCTGCCGCCGGAGCTGTTCTGCGGATATTATTTCTGCAAGA
CGCAGGAAGAAGAACATTTCGCCCGCGCGTTCGAGTGGCAGGACCATCCGGCCGTTCATCAGCTCTCGGATCTTGCCGCA
GAACTTGGCGTCGTCATTCCGGTCTCTATCTACGAGAAGGAAGGCCCGCACTATTTCAACTCCATCGTCGTGATTGACGC
GGACGGCGCGCCGCTTGGCGTTTATCGCAAGAGCCACATTCCCGATGGTCCCGGCTATCAGGAGAAGTATTATTTCCGGC
CGGGCGATACCGGCTTCCGGGTCTGGAACACAATGAAGGGCCGGATCGGCGTGGGCATCTGCTGGGATCAATGGTTCCCC
GAAGCGGCCCGTTCGATGGCGCTCATGGGCGCCGATGTGCTGCTCTATCCCACCGCGATTGGCGCAGAGCCGCAGGACGC
CTCTCTGGACACCGCCGCGCGCTGGCGCCGGGGCATGCAGGGCCATGCGGTCGCAAACGTCATCCCGGTGGTTGCCGCAA
ACCGGGTCGGGGACGAGCAGGGGCAGGTTTTCTACGGCACCAGCTTCATCACCGATGAAACCGGCGAGATTGCCGCCGAT
CTGGACCGGAAGGAAGAAGGCGTGATTGTTGCCACCTTCGATCTGGAGCATATCGACCGGGCAAGAGCCGCCTGGGGCTT
CTTCCGTGATCGGAGAACCGATCTCTACGAGATACTCGTCTAG

Upstream 100 bases:

>100_bases
CGGGAAGTGATTGGCCTGCCGGCGCGTGCTATCCTCGCGGGCGGCGGCAGTTTCCACTGCATGACCCGCGAAATTCCTGC
CTGACAAGGAGCCCAAGCTC

Downstream 100 bases:

>100_bases
CCGCCGGCCCCGCAATCAGCGGCGCTGGGATGTACCGAACATCTTGGCGACGCGTTCTTCGGTATCATCATTTTCTGCAA
AGACGACCGGCTCACTGCTC

Product: carbon-nitrogen family hydrolase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MTRTITLAAIQFTPTDDVQENIDRVAGFVREAAAKGADVILPPELFCGYYFCKTQEEEHFARAFEWQDHPAVHQLSDLAA
ELGVVIPVSIYEKEGPHYFNSIVVIDADGAPLGVYRKSHIPDGPGYQEKYYFRPGDTGFRVWNTMKGRIGVGICWDQWFP
EAARSMALMGADVLLYPTAIGAEPQDASLDTAARWRRGMQGHAVANVIPVVAANRVGDEQGQVFYGTSFITDETGEIAAD
LDRKEEGVIVATFDLEHIDRARAAWGFFRDRRTDLYEILV

Sequences:

>Translated_280_residues
MTRTITLAAIQFTPTDDVQENIDRVAGFVREAAAKGADVILPPELFCGYYFCKTQEEEHFARAFEWQDHPAVHQLSDLAA
ELGVVIPVSIYEKEGPHYFNSIVVIDADGAPLGVYRKSHIPDGPGYQEKYYFRPGDTGFRVWNTMKGRIGVGICWDQWFP
EAARSMALMGADVLLYPTAIGAEPQDASLDTAARWRRGMQGHAVANVIPVVAANRVGDEQGQVFYGTSFITDETGEIAAD
LDRKEEGVIVATFDLEHIDRARAAWGFFRDRRTDLYEILV
>Mature_279_residues
TRTITLAAIQFTPTDDVQENIDRVAGFVREAAAKGADVILPPELFCGYYFCKTQEEEHFARAFEWQDHPAVHQLSDLAAE
LGVVIPVSIYEKEGPHYFNSIVVIDADGAPLGVYRKSHIPDGPGYQEKYYFRPGDTGFRVWNTMKGRIGVGICWDQWFPE
AARSMALMGADVLLYPTAIGAEPQDASLDTAARWRRGMQGHAVANVIPVVAANRVGDEQGQVFYGTSFITDETGEIAADL
DRKEEGVIVATFDLEHIDRARAAWGFFRDRRTDLYEILV

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=280, Percent_Identity=31.7857142857143, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI297632350, Length=283, Percent_Identity=28.9752650176678, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI297632348, Length=283, Percent_Identity=28.9752650176678, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI5031947, Length=283, Percent_Identity=28.9752650176678, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI7706509, Length=274, Percent_Identity=28.1021897810219, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI297632346, Length=188, Percent_Identity=29.7872340425532, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17533173, Length=303, Percent_Identity=31.3531353135314, Blast_Score=111, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17556280, Length=285, Percent_Identity=25.6140350877193, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323383, Length=289, Percent_Identity=26.2975778546713, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6322335, Length=314, Percent_Identity=25.1592356687898, Blast_Score=79, Evalue=7e-16,
Organism=Drosophila melanogaster, GI21358471, Length=305, Percent_Identity=28.8524590163934, Blast_Score=111, Evalue=5e-25,
Organism=Drosophila melanogaster, GI17933642, Length=284, Percent_Identity=27.112676056338, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 31237; Mature: 31106

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTITLAAIQFTPTDDVQENIDRVAGFVREAAAKGADVILPPELFCGYYFCKTQEEEHF
CCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHCCCEECCCCHHHH
ARAFEWQDHPAVHQLSDLAAELGVVIPVSIYEKEGPHYFNSIVVIDADGAPLGVYRKSHI
HHHHCCCCCCHHHHHHHHHHHCCEEEEEEEEECCCCCEEEEEEEEECCCCCEECEECCCC
PDGPGYQEKYYFRPGDTGFRVWNTMKGRIGVGICWDQWFPEAARSMALMGADVLLYPTAI
CCCCCCCCEEEECCCCCCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHCCCEEEEEEEC
GAEPQDASLDTAARWRRGMQGHAVANVIPVVAANRVGDEQGQVFYGTSFITDETGEIAAD
CCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCEEECCCCCCHHHC
LDRKEEGVIVATFDLEHIDRARAAWGFFRDRRTDLYEILV
CCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCHHHHHCC
>Mature Secondary Structure 
TRTITLAAIQFTPTDDVQENIDRVAGFVREAAAKGADVILPPELFCGYYFCKTQEEEHF
CEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHCCCEECCCCHHHH
ARAFEWQDHPAVHQLSDLAAELGVVIPVSIYEKEGPHYFNSIVVIDADGAPLGVYRKSHI
HHHHCCCCCCHHHHHHHHHHHCCEEEEEEEEECCCCCEEEEEEEEECCCCCEECEECCCC
PDGPGYQEKYYFRPGDTGFRVWNTMKGRIGVGICWDQWFPEAARSMALMGADVLLYPTAI
CCCCCCCCEEEECCCCCCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHCCCEEEEEEEC
GAEPQDASLDTAARWRRGMQGHAVANVIPVVAANRVGDEQGQVFYGTSFITDETGEIAAD
CCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCEEECCCCCCHHHC
LDRKEEGVIVATFDLEHIDRARAAWGFFRDRRTDLYEILV
CCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]