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The map label for this gene is aes [H]

Identifier: 114799294

GI number: 114799294

Start: 2434306

End: 2435265

Strand: Direct

Name: aes [H]

Synonym: HNE_2331

Alternate gene names: 114799294

Gene position: 2434306-2435265 (Clockwise)

Preceding gene: 114800312

Following gene: 114798709

Centisome position: 65.7

GC content: 68.54

Gene sequence:

>960_bases
ATGAGCGTTTCGCCCTTGTCCTGCGCGGATGAACTTGATCCCGGCGTGCGCCGCTTTATCGAGCGCACCGGCGCGGATTA
TACGGCCCTCTCCACCGTCGCGCCGCCCACTACGCTTGCGCGCCGGCGCGAGATTGCCGAACAGGTGCGCCAGCCCTGGT
CGCGTGGCGGCCCGGATATGGCCCAGACCCATATGGTCGCCATCGGCCCGCAGGGCGTGCGCGCCCGCGTCCACATCCCC
GCCAGCGGCCCCGGCACCGGCACGCTGCTTTACCTCCACGGTGGCGGCTGGACCCTCTTCAGCATCGACACGCATGACCG
CCTGATGCGCGAATATGCGGAGCGTATCGGGTGCGCCGTCGTCGGCCTCGACTATTCCCTCTCCCCCGAACACCGCTTCC
CCCGCGCGCTTGAAGATGTCGATGCCTGCATCGAATGGCTCCGCCGCGAGGGCGCCGCGCTTGGCCTGGCGGTAGACCGC
CTCGCCATCGGCGGGGATTCTGCGGGCGGAAACCTCTCCCTGTCCGCCGCGCTGCGCCTGCGGGATCGCGGCGAGCCGCT
CCCCGCCGCCCTCCTCCTCAACTACGCCGCCCTCGACACCGAGCCGCGCCCCTCCTACCGGCGCTATGACGGCGACCCCT
ACATGCTGAATGTCGACGAGATGCGCGACTTCTGGGTCAACTATCTCGGCAAACCGTCTACGGACGATCCCTATGCCCGC
CCGCTGCTGGCAGACCTGACCGGCCTGCCGCCCGTCCATCTCTGCATCGCCCAATGCGACATCCTCGCCGACCAGAACAC
CGAACTCGCCGCCCGCCTCGAAGCGGTCGGCAACGACGTCTCCACCCGCATCTATGAAGGCGCCACCCACTCCTTCCTCG
AAGCCGTCAGCATTTCCGACTGCGCAGACCGGGCCATTCAGGACGCCTCAGACTGGCTCGCCCGCCACCTCACCCCATGA

Upstream 100 bases:

>100_bases
CGAAGAAGAAGGCCGCCAGCGCCGCCCCGGCCGAAGCCTGAACCTTTCCCTCACGGGCCGGCACGCCCATAAGAACAACA
CAAACAGAAGAGCCTTATCC

Downstream 100 bases:

>100_bases
GCGCCCGCTTCACCACCGTTCCGGACACAGACATCCTCCGCTTCACAGCGGAAAACCCGCTCGCCTGGGTCGTGCCCGCC
GCTGATCCGGGCGATGCCAT

Product: alpha/beta fold family hydrolase

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MSVSPLSCADELDPGVRRFIERTGADYTALSTVAPPTTLARRREIAEQVRQPWSRGGPDMAQTHMVAIGPQGVRARVHIP
ASGPGTGTLLYLHGGGWTLFSIDTHDRLMREYAERIGCAVVGLDYSLSPEHRFPRALEDVDACIEWLRREGAALGLAVDR
LAIGGDSAGGNLSLSAALRLRDRGEPLPAALLLNYAALDTEPRPSYRRYDGDPYMLNVDEMRDFWVNYLGKPSTDDPYAR
PLLADLTGLPPVHLCIAQCDILADQNTELAARLEAVGNDVSTRIYEGATHSFLEAVSISDCADRAIQDASDWLARHLTP

Sequences:

>Translated_319_residues
MSVSPLSCADELDPGVRRFIERTGADYTALSTVAPPTTLARRREIAEQVRQPWSRGGPDMAQTHMVAIGPQGVRARVHIP
ASGPGTGTLLYLHGGGWTLFSIDTHDRLMREYAERIGCAVVGLDYSLSPEHRFPRALEDVDACIEWLRREGAALGLAVDR
LAIGGDSAGGNLSLSAALRLRDRGEPLPAALLLNYAALDTEPRPSYRRYDGDPYMLNVDEMRDFWVNYLGKPSTDDPYAR
PLLADLTGLPPVHLCIAQCDILADQNTELAARLEAVGNDVSTRIYEGATHSFLEAVSISDCADRAIQDASDWLARHLTP
>Mature_318_residues
SVSPLSCADELDPGVRRFIERTGADYTALSTVAPPTTLARRREIAEQVRQPWSRGGPDMAQTHMVAIGPQGVRARVHIPA
SGPGTGTLLYLHGGGWTLFSIDTHDRLMREYAERIGCAVVGLDYSLSPEHRFPRALEDVDACIEWLRREGAALGLAVDRL
AIGGDSAGGNLSLSAALRLRDRGEPLPAALLLNYAALDTEPRPSYRRYDGDPYMLNVDEMRDFWVNYLGKPSTDDPYARP
LLADLTGLPPVHLCIAQCDILADQNTELAARLEAVGNDVSTRIYEGATHSFLEAVSISDCADRAIQDASDWLARHLTP

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21328446, Length=130, Percent_Identity=34.6153846153846, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI68299767, Length=185, Percent_Identity=30.2702702702703, Blast_Score=75, Evalue=9e-14,
Organism=Escherichia coli, GI1786682, Length=285, Percent_Identity=32.280701754386, Blast_Score=134, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI71996133, Length=316, Percent_Identity=25.9493670886076, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17540028, Length=312, Percent_Identity=25.6410256410256, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17567059, Length=287, Percent_Identity=25.7839721254355, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI115533412, Length=162, Percent_Identity=31.4814814814815, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI115533410, Length=162, Percent_Identity=31.4814814814815, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI72001146, Length=176, Percent_Identity=30.1136363636364, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24656084, Length=112, Percent_Identity=38.3928571428571, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24656076, Length=112, Percent_Identity=38.3928571428571, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI20130169, Length=112, Percent_Identity=38.3928571428571, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 34832; Mature: 34700

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSPLSCADELDPGVRRFIERTGADYTALSTVAPPTTLARRREIAEQVRQPWSRGGPDM
CCCCCCCCHHHCCHHHHHHHHHCCCCCEEHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCH
AQTHMVAIGPQGVRARVHIPASGPGTGTLLYLHGGGWTLFSIDTHDRLMREYAERIGCAV
HHCEEEEECCCCCEEEEEECCCCCCCEEEEEEECCCEEEEEECHHHHHHHHHHHHHCEEE
VGLDYSLSPEHRFPRALEDVDACIEWLRREGAALGLAVDRLAIGGDSAGGNLSLSAALRL
EECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEHHHEEECCCCCCCCEEEEEEEEE
RDRGEPLPAALLLNYAALDTEPRPSYRRYDGDPYMLNVDEMRDFWVNYLGKPSTDDPYAR
CCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCHHHH
PLLADLTGLPPVHLCIAQCDILADQNTELAARLEAVGNDVSTRIYEGATHSFLEAVSISD
HHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHH
CADRAIQDASDWLARHLTP
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SVSPLSCADELDPGVRRFIERTGADYTALSTVAPPTTLARRREIAEQVRQPWSRGGPDM
CCCCCCCHHHCCHHHHHHHHHCCCCCEEHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCH
AQTHMVAIGPQGVRARVHIPASGPGTGTLLYLHGGGWTLFSIDTHDRLMREYAERIGCAV
HHCEEEEECCCCCEEEEEECCCCCCCEEEEEEECCCEEEEEECHHHHHHHHHHHHHCEEE
VGLDYSLSPEHRFPRALEDVDACIEWLRREGAALGLAVDRLAIGGDSAGGNLSLSAALRL
EECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEHHHEEECCCCCCCCEEEEEEEEE
RDRGEPLPAALLLNYAALDTEPRPSYRRYDGDPYMLNVDEMRDFWVNYLGKPSTDDPYAR
CCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCHHHH
PLLADLTGLPPVHLCIAQCDILADQNTELAARLEAVGNDVSTRIYEGATHSFLEAVSISD
HHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHH
CADRAIQDASDWLARHLTP
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA