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The map label for this gene is cca [H]

Identifier: 114799278

GI number: 114799278

Start: 340236

End: 341381

Strand: Direct

Name: cca [H]

Synonym: HNE_0346

Alternate gene names: 114799278

Gene position: 340236-341381 (Clockwise)

Preceding gene: 114798320

Following gene: 114800278

Centisome position: 9.18

GC content: 69.55

Gene sequence:

>1146_bases
ATGGCCGCGCTTGACGCGGCCCGCGCCGGCGCGTCCCGTTATGTCGGCGGATCGGTGCGCAACACCCTGCGCGGCCTGAA
GGCTGAGGATGTCGACATCGCCACCCAGCTGCTCCCGCAGGAGACCGCCGCCGCGCTGGAAGCTGCCGGTATCCGCGTCA
TTCCCACAGGCATCGAGCACGGCACCGTCACCGCCTTCTACAAGGGCGTGCCCATCGAGATCACCACCCTGCGCCGGGAT
GTCGAAACCGATGGCCGCCGCGCCGTTGTCGCCTTCACCCAGGATTGGGCCGAAGACGCCGCCCGCCGCGACTTCCGCAT
GAACGCCATCTATGCCGGCGCGGAGGGGGAACTTTACGAAGTCATCCCCGGCTCCATCGACGACGCCATTGCTGGTCGCG
TCATCTTCATCGGCGAGGGCGACCAGCGCCTGATCGAAGACTATCTGCGCATCCTGCGCTTCTACCGGTTCAACGCCTGG
TATGGCGCGGGCATCGATGAAGACGGGCAGGGCGCCTGCGCCCGCCAGCGCGAAGGCCTCCGGAAGATTGCCGCGGAACG
GATCTGGAAAGAGCTCAAACGCACGCTCGCCGCGCCCGATCCCGCGCCCGCCATGGCCGCGATGGAAGAGGCCGGCGTGC
TCGAAATCCTCCTGCCGGGTGCCAGCGCCGCCCTGTTGCCCGGTCTGGCCGCGGTTGAAGCCGCCAATGGCCTGGCGCCT
GATCCGATGCAGCGCCTCATGGCCCTTCTGCCCCGCCGTCAGCGCGACGTGACAGACGCGGCCAATGCCCTGCGCCTCTC
CAATCAGGAAGCCGCCCGCCTGCAAGCCTGGGCCGACCCCGCGCTCGGCCATGTCCTCGCCCTCGGCCCGGCCCCCCGGC
GCGAAGCCTGCTACCGCTTCACCCCCCAGGCCGTCCTCGACCGCGCCCTCATCGAAGCGGCCAGCGGCGGGGAGGGCAGC
CTCACGCCGCTCTTGCAGGAGAAGGCCCACTGGATGCGCCCCGTCTTCCCCATCGGCGGACAGGACGCGCTCGCCGCCGG
CCTCAAAGGCCCCGACATCGGCAAGCGGCTCTACACGCTGGAACAGGCCTGGATCGCGTCCGGCTTCCGCCTTTCACGGG
AAGACCTGCTCTCGCGCCTGAAATGA

Upstream 100 bases:

>100_bases
CTCACCAACCCGTCCGAGACCCCGGCTCAAACCCCCGCCGATGACCCGACCTGACCGCATAACGGCAGACTGGCTCACCG
CGCCCGCTAGCCGGGCGGTC

Downstream 100 bases:

>100_bases
CGCCCGGCGTGTCCTGATTTGTCCTGACCTGTCCCGGCTTGTCCGTAAAATGACTGTCAGAGGTGCCCTTGGTTCCCAGA
CAGGCCGTCCCATGTTATAA

Product: tRNA nucleotidyltransferase/poly(A) polymerase family protein

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MAALDAARAGASRYVGGSVRNTLRGLKAEDVDIATQLLPQETAAALEAAGIRVIPTGIEHGTVTAFYKGVPIEITTLRRD
VETDGRRAVVAFTQDWAEDAARRDFRMNAIYAGAEGELYEVIPGSIDDAIAGRVIFIGEGDQRLIEDYLRILRFYRFNAW
YGAGIDEDGQGACARQREGLRKIAAERIWKELKRTLAAPDPAPAMAAMEEAGVLEILLPGASAALLPGLAAVEAANGLAP
DPMQRLMALLPRRQRDVTDAANALRLSNQEAARLQAWADPALGHVLALGPAPRREACYRFTPQAVLDRALIEAASGGEGS
LTPLLQEKAHWMRPVFPIGGQDALAAGLKGPDIGKRLYTLEQAWIASGFRLSREDLLSRLK

Sequences:

>Translated_381_residues
MAALDAARAGASRYVGGSVRNTLRGLKAEDVDIATQLLPQETAAALEAAGIRVIPTGIEHGTVTAFYKGVPIEITTLRRD
VETDGRRAVVAFTQDWAEDAARRDFRMNAIYAGAEGELYEVIPGSIDDAIAGRVIFIGEGDQRLIEDYLRILRFYRFNAW
YGAGIDEDGQGACARQREGLRKIAAERIWKELKRTLAAPDPAPAMAAMEEAGVLEILLPGASAALLPGLAAVEAANGLAP
DPMQRLMALLPRRQRDVTDAANALRLSNQEAARLQAWADPALGHVLALGPAPRREACYRFTPQAVLDRALIEAASGGEGS
LTPLLQEKAHWMRPVFPIGGQDALAAGLKGPDIGKRLYTLEQAWIASGFRLSREDLLSRLK
>Mature_380_residues
AALDAARAGASRYVGGSVRNTLRGLKAEDVDIATQLLPQETAAALEAAGIRVIPTGIEHGTVTAFYKGVPIEITTLRRDV
ETDGRRAVVAFTQDWAEDAARRDFRMNAIYAGAEGELYEVIPGSIDDAIAGRVIFIGEGDQRLIEDYLRILRFYRFNAWY
GAGIDEDGQGACARQREGLRKIAAERIWKELKRTLAAPDPAPAMAAMEEAGVLEILLPGASAALLPGLAAVEAANGLAPD
PMQRLMALLPRRQRDVTDAANALRLSNQEAARLQAWADPALGHVLALGPAPRREACYRFTPQAVLDRALIEAASGGEGSL
TPLLQEKAHWMRPVFPIGGQDALAAGLKGPDIGKRLYTLEQAWIASGFRLSREDLLSRLK

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=394, Percent_Identity=33.248730964467, Blast_Score=188, Evalue=9e-48,
Organism=Caenorhabditis elegans, GI71995920, Length=399, Percent_Identity=31.328320802005, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI281360112, Length=379, Percent_Identity=31.3984168865435, Blast_Score=158, Evalue=5e-39,
Organism=Drosophila melanogaster, GI21357337, Length=379, Percent_Identity=31.3984168865435, Blast_Score=158, Evalue=5e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 41139; Mature: 41007

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAALDAARAGASRYVGGSVRNTLRGLKAEDVDIATQLLPQETAAALEAAGIRVIPTGIEH
CCCCHHHHCCCHHCCCHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHCCEEEEECCCCC
GTVTAFYKGVPIEITTLRRDVETDGRRAVVAFTQDWAEDAARRDFRMNAIYAGAEGELYE
CCEEEEECCCCEEEEEEHHHHCCCCCEEEEEECHHHHHHHHHCCEEEEEEEECCCCCEEE
VIPGSIDDAIAGRVIFIGEGDQRLIEDYLRILRFYRFNAWYGAGIDEDGQGACARQREGL
ECCCCCCHHHCCEEEEEECCCHHHHHHHHHHHHHHHHCCEECCCCCCCCCCHHHHHHHHH
RKIAAERIWKELKRTLAAPDPAPAMAAMEEAGVLEILLPGASAALLPGLAAVEAANGLAP
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCEEEEEECCCCHHHHCCHHHHHHHCCCCC
DPMQRLMALLPRRQRDVTDAANALRLSNQEAARLQAWADPALGHVLALGPAPRREACYRF
CHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHEEECCCCCCHHHHHCC
TPQAVLDRALIEAASGGEGSLTPLLQEKAHWMRPVFPIGGQDALAAGLKGPDIGKRLYTL
CHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCHHHHHHHH
EQAWIASGFRLSREDLLSRLK
HHHHHHCCCCCCHHHHHHHCC
>Mature Secondary Structure 
AALDAARAGASRYVGGSVRNTLRGLKAEDVDIATQLLPQETAAALEAAGIRVIPTGIEH
CCCHHHHCCCHHCCCHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHCCEEEEECCCCC
GTVTAFYKGVPIEITTLRRDVETDGRRAVVAFTQDWAEDAARRDFRMNAIYAGAEGELYE
CCEEEEECCCCEEEEEEHHHHCCCCCEEEEEECHHHHHHHHHCCEEEEEEEECCCCCEEE
VIPGSIDDAIAGRVIFIGEGDQRLIEDYLRILRFYRFNAWYGAGIDEDGQGACARQREGL
ECCCCCCHHHCCEEEEEECCCHHHHHHHHHHHHHHHHCCEECCCCCCCCCCHHHHHHHHH
RKIAAERIWKELKRTLAAPDPAPAMAAMEEAGVLEILLPGASAALLPGLAAVEAANGLAP
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCEEEEEECCCCHHHHCCHHHHHHHCCCCC
DPMQRLMALLPRRQRDVTDAANALRLSNQEAARLQAWADPALGHVLALGPAPRREACYRF
CHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHEEECCCCCCHHHHHCC
TPQAVLDRALIEAASGGEGSLTPLLQEKAHWMRPVFPIGGQDALAAGLKGPDIGKRLYTL
CHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCHHHHHHHH
EQAWIASGFRLSREDLLSRLK
HHHHHHCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA