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The map label for this gene is ptsP [H]

Identifier: 114798877

GI number: 114798877

Start: 619072

End: 621339

Strand: Direct

Name: ptsP [H]

Synonym: HNE_0619

Alternate gene names: 114798877

Gene position: 619072-621339 (Clockwise)

Preceding gene: 114798362

Following gene: 114799427

Centisome position: 16.71

GC content: 65.26

Gene sequence:

>2268_bases
ATGCCGTATAACCTGCCGGCCACCCGAGTTCTGATGAACAACCTCCGCCAGGTGATGGCAGAGGAGGGTGACGCACGTTC
GCGCCTCGATCAGGTGGTGCGCATCATTGCCTCCACCATGGTCGCCGATGTGTGCTCGATCTATCACCGCGAGGCCGACG
AGACGCTGCTGCTGATCGCCACCGAGGGCCTGAAGGAAGAGGCCGTCAACAAGACCCGCATGGGCGCCAATGAGGGCCTT
GTCGGCTATGTCGCCCGCACGGCAGAGCCCATCGCGATTGAAGACGCCCCGCGCCACCCGGCCTTCTCCTACCGCCCGGA
AACCGGCGAAGACCCGTTCCATGCCTTCCTCGGCGTGCCGATCCTGCGCACTGGCGGCGTGCTCGGCGTGCTCGTCGTGC
AGAACCGCACCGAGCGCACCTATCGCGACGAGGAAATCGAAACCCTCCAGACTATTGCCATGGTGCTGGCCGAGATCGTC
CACCGCATTGAGGAAGACCAGTCTACGCCCCAGCCTGACCGGCGCTTCCGGGGCCAGAGCAGCGAAAACCTGCGCGGGCG
CATCTTCTGCGAAGGCCTCGGCTATGGCCGGGCCGTGCTGCACGATCCGGTGGTTCCGGCCGCCCGCTTCTTTGCCGCCG
ACCAGAAACAGGAAACCCTGCGGCTGACGCAGGGCCTGAAGGACCTGCGCACCTCGATTGACCGGATGATGCTGATCGAC
GGCGCCGCACTGGGCGAAGACCCGCGCGATGTGATGGAGGCCTACCGCCTTCTGGCGCATGACAATTCCTGGGCCGAAAA
GTTGCAGGAAGGTGTGCGTTCGGGCCTCTCCGCTGAAGCCTCGGTCGACCGCGCCCGCCGCGAACACCGCGCCAAGATGC
AGGCCGCGCGCGATCCCTATCTGCGCGAACGCCTGCATGACCTGGAAGACCTCGACAACCGGCTGCTGCGTATCCTCGGC
GGGGACGAGAACAAGCCCCAGATCACCGATGAGAGCAGCGTGCTGGTCGCCCGCCGTCTCGGCCCGGCAGAGCTTCTGGA
ATACGCCAAGACCGGCCTTAAAGGCATCCTGATGGAAGAGGCGGCCGCCTCCTCCCACGCCGCCATCGTGGCGCGCGCGC
TCAGCATTCCGGCCATCGGCGGCATCGAGGGGCTCACCACCCTGATCGAGCAGGGCGATTATGTGATCGTCGACGCCGAA
CAGGGCCAGGCGCACATCCGGCCCGATGACGCCGTCTATGACGCCTACAAGTCCCGCGTGGCGCTCCGCTCGGAGCGTCA
GGCAGTCTATGCCAGCCTCAAGGGGCTCCCCGCGCAGACAAAAGACGGCGAAACCCTCTCGCTGATGCTGAACGCCGGCC
TCGACCTTGATCTCGACATGCTCGATCAGGCCGGCGCCGACGGGGTCGGCCTCTTCCGCACCGAGTTCCAGTTCCTCGTC
TCCGAAAGCCTGCCGCGCCTGGAAGACCAGATTTCGCTCTATCGCCGCGTGCTGGACCGGGCGGCGGGACGGCCCGTCAT
CTTCCGGACGCTCGATCTGGGCGGCGACAAGGTTCTGCCCATTCTCAACCAGCTGCGCGAAGAAAACCCGGCGCTCGGCT
GGCGCTCGATCCGCTTCGCGCTCGACCATAAAGGTCTCTTCCGCCGCCAGCTGCGCGCGCTTGTCCGGGCGGCCGGGGAA
GGGCCCCTGACGGTCATGTTCCCCATGGTGACGACGCCCGAAGAGTTCCGCGAGGCGCGCGCGTTGCTGGAGGAAGAGGT
CGCCTGGACCCTGAAACGCACCGGCAAGACGCCGTCAAAGCTCGAAATCGGCGTGATGCTGGAAACCCCGGCGCTGGCTT
TCTCGCTGTCGGATCTCGCCGGTGAGGTCGATTTCCTCTCCGTCGGCACCAACGATCTGTTGCAGTTCTTCTTTGCCGCT
GACCGGATGACGCCGTCTGTGTCAGACCGGTACGCTTTGATCAGCCCGGCGGCCCTGCGTTTCCTGCGGATGGTGGCGGA
GACGTGCAAGACCGCCGGTATCCGGCTCTCGGTCTGCGGGGAGGCAACCGCCACCCCGCTGGCCGCTTTCTGCTTTGCTG
CCTTCGGTTACAGCAGCCTGTCGATGTCGGCTTCGTCCATCGGTCCGGTCAAACAGGCCCTCAGATCCATTGATCTGGCC
GGATTCCGCAAGGGTCTGGACGCGGCGCTGGAGAAGCCGAACGGTGCGTTCCGTAACCAAGTATTAGGGATCGCCATTGA
ACATGGGGCCGATATACCGGACACATGA

Upstream 100 bases:

>100_bases
CGTTCTGATCGCCGCGCCCTATACAGAACTGGCGGTCCGGGCACTTCATACAGCATTCGGGCTGGACGCAGACTGACGGG
TCACCGTAGACCGGGAGAGC

Downstream 100 bases:

>100_bases
AGCTGGTTATGACGGGATGATGGCGCATTTGTCTTGGCCTGGACCGTTTTTTGCCAAATTTGGTACCCATATGGCAGCGG
TCGAAGTGACTGGTAGAATA

Product: phosphoenolpyruvate-protein phosphotransferase PtsP

Products: NA

Alternate protein names: Enzyme I-Ntr; Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 755; Mature: 754

Protein sequence:

>755_residues
MPYNLPATRVLMNNLRQVMAEEGDARSRLDQVVRIIASTMVADVCSIYHREADETLLLIATEGLKEEAVNKTRMGANEGL
VGYVARTAEPIAIEDAPRHPAFSYRPETGEDPFHAFLGVPILRTGGVLGVLVVQNRTERTYRDEEIETLQTIAMVLAEIV
HRIEEDQSTPQPDRRFRGQSSENLRGRIFCEGLGYGRAVLHDPVVPAARFFAADQKQETLRLTQGLKDLRTSIDRMMLID
GAALGEDPRDVMEAYRLLAHDNSWAEKLQEGVRSGLSAEASVDRARREHRAKMQAARDPYLRERLHDLEDLDNRLLRILG
GDENKPQITDESSVLVARRLGPAELLEYAKTGLKGILMEEAAASSHAAIVARALSIPAIGGIEGLTTLIEQGDYVIVDAE
QGQAHIRPDDAVYDAYKSRVALRSERQAVYASLKGLPAQTKDGETLSLMLNAGLDLDLDMLDQAGADGVGLFRTEFQFLV
SESLPRLEDQISLYRRVLDRAAGRPVIFRTLDLGGDKVLPILNQLREENPALGWRSIRFALDHKGLFRRQLRALVRAAGE
GPLTVMFPMVTTPEEFREARALLEEEVAWTLKRTGKTPSKLEIGVMLETPALAFSLSDLAGEVDFLSVGTNDLLQFFFAA
DRMTPSVSDRYALISPAALRFLRMVAETCKTAGIRLSVCGEATATPLAAFCFAAFGYSSLSMSASSIGPVKQALRSIDLA
GFRKGLDAALEKPNGAFRNQVLGIAIEHGADIPDT

Sequences:

>Translated_755_residues
MPYNLPATRVLMNNLRQVMAEEGDARSRLDQVVRIIASTMVADVCSIYHREADETLLLIATEGLKEEAVNKTRMGANEGL
VGYVARTAEPIAIEDAPRHPAFSYRPETGEDPFHAFLGVPILRTGGVLGVLVVQNRTERTYRDEEIETLQTIAMVLAEIV
HRIEEDQSTPQPDRRFRGQSSENLRGRIFCEGLGYGRAVLHDPVVPAARFFAADQKQETLRLTQGLKDLRTSIDRMMLID
GAALGEDPRDVMEAYRLLAHDNSWAEKLQEGVRSGLSAEASVDRARREHRAKMQAARDPYLRERLHDLEDLDNRLLRILG
GDENKPQITDESSVLVARRLGPAELLEYAKTGLKGILMEEAAASSHAAIVARALSIPAIGGIEGLTTLIEQGDYVIVDAE
QGQAHIRPDDAVYDAYKSRVALRSERQAVYASLKGLPAQTKDGETLSLMLNAGLDLDLDMLDQAGADGVGLFRTEFQFLV
SESLPRLEDQISLYRRVLDRAAGRPVIFRTLDLGGDKVLPILNQLREENPALGWRSIRFALDHKGLFRRQLRALVRAAGE
GPLTVMFPMVTTPEEFREARALLEEEVAWTLKRTGKTPSKLEIGVMLETPALAFSLSDLAGEVDFLSVGTNDLLQFFFAA
DRMTPSVSDRYALISPAALRFLRMVAETCKTAGIRLSVCGEATATPLAAFCFAAFGYSSLSMSASSIGPVKQALRSIDLA
GFRKGLDAALEKPNGAFRNQVLGIAIEHGADIPDT
>Mature_754_residues
PYNLPATRVLMNNLRQVMAEEGDARSRLDQVVRIIASTMVADVCSIYHREADETLLLIATEGLKEEAVNKTRMGANEGLV
GYVARTAEPIAIEDAPRHPAFSYRPETGEDPFHAFLGVPILRTGGVLGVLVVQNRTERTYRDEEIETLQTIAMVLAEIVH
RIEEDQSTPQPDRRFRGQSSENLRGRIFCEGLGYGRAVLHDPVVPAARFFAADQKQETLRLTQGLKDLRTSIDRMMLIDG
AALGEDPRDVMEAYRLLAHDNSWAEKLQEGVRSGLSAEASVDRARREHRAKMQAARDPYLRERLHDLEDLDNRLLRILGG
DENKPQITDESSVLVARRLGPAELLEYAKTGLKGILMEEAAASSHAAIVARALSIPAIGGIEGLTTLIEQGDYVIVDAEQ
GQAHIRPDDAVYDAYKSRVALRSERQAVYASLKGLPAQTKDGETLSLMLNAGLDLDLDMLDQAGADGVGLFRTEFQFLVS
ESLPRLEDQISLYRRVLDRAAGRPVIFRTLDLGGDKVLPILNQLREENPALGWRSIRFALDHKGLFRRQLRALVRAAGEG
PLTVMFPMVTTPEEFREARALLEEEVAWTLKRTGKTPSKLEIGVMLETPALAFSLSDLAGEVDFLSVGTNDLLQFFFAAD
RMTPSVSDRYALISPAALRFLRMVAETCKTAGIRLSVCGEATATPLAAFCFAAFGYSSLSMSASSIGPVKQALRSIDLAG
FRKGLDAALEKPNGAFRNQVLGIAIEHGADIPDT

Specific function: Component of the phosphoenolpyruvate-dependent nitrogen- metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. Enzyme I- Ntr transfers the phosphoryl group from phosphoenolpyruvate (PEP)

COG id: COG3605

COG function: function code T; Signal transduction protein containing GAF and PtsI domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GAF domain [H]

Homologues:

Organism=Escherichia coli, GI1789193, Length=716, Percent_Identity=35.0558659217877, Blast_Score=366, Evalue=1e-102,
Organism=Escherichia coli, GI1788756, Length=557, Percent_Identity=33.572710951526, Blast_Score=297, Evalue=2e-81,
Organism=Escherichia coli, GI1788726, Length=550, Percent_Identity=32, Blast_Score=259, Evalue=5e-70,
Organism=Escherichia coli, GI48994992, Length=568, Percent_Identity=32.2183098591549, Blast_Score=246, Evalue=3e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003018
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF01590 GAF; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 83086; Mature: 82955

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYNLPATRVLMNNLRQVMAEEGDARSRLDQVVRIIASTMVADVCSIYHREADETLLLIA
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
TEGLKEEAVNKTRMGANEGLVGYVARTAEPIAIEDAPRHPAFSYRPETGEDPFHAFLGVP
CCCCHHHHHHHHHCCCCCCCHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHCCH
ILRTGGVLGVLVVQNRTERTYRDEEIETLQTIAMVLAEIVHRIEEDQSTPQPDRRFRGQS
HHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCC
SENLRGRIFCEGLGYGRAVLHDPVVPAARFFAADQKQETLRLTQGLKDLRTSIDRMMLID
CCCCCEEEEEECCCCCHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
GAALGEDPRDVMEAYRLLAHDNSWAEKLQEGVRSGLSAEASVDRARREHRAKMQAARDPY
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHH
LRERLHDLEDLDNRLLRILGGDENKPQITDESSVLVARRLGPAELLEYAKTGLKGILMEE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHCHHHHHHHH
AAASSHAAIVARALSIPAIGGIEGLTTLIEQGDYVIVDAEQGQAHIRPDDAVYDAYKSRV
HHCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHH
ALRSERQAVYASLKGLPAQTKDGETLSLMLNAGLDLDLDMLDQAGADGVGLFRTEFQFLV
HHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCHHHHHHCCCCCCHHHHHHHHHHH
SESLPRLEDQISLYRRVLDRAAGRPVIFRTLDLGGDKVLPILNQLREENPALGWRSIRFA
HHCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHH
LDHKGLFRRQLRALVRAAGEGPLTVMFPMVTTPEEFREARALLEEEVAWTLKRTGKTPSK
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LEIGVMLETPALAFSLSDLAGEVDFLSVGTNDLLQFFFAADRMTPSVSDRYALISPAALR
EEEEEEEECCHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCEEHCCHHHHH
FLRMVAETCKTAGIRLSVCGEATATPLAAFCFAAFGYSSLSMSASSIGPVKQALRSIDLA
HHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHCHHHHHCCHHCCCHHHHHHHHHHHH
GFRKGLDAALEKPNGAFRNQVLGIAIEHGADIPDT
HHHHHHHHHHCCCCCHHHHHHEEEEEECCCCCCCC
>Mature Secondary Structure 
PYNLPATRVLMNNLRQVMAEEGDARSRLDQVVRIIASTMVADVCSIYHREADETLLLIA
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
TEGLKEEAVNKTRMGANEGLVGYVARTAEPIAIEDAPRHPAFSYRPETGEDPFHAFLGVP
CCCCHHHHHHHHHCCCCCCCHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHCCH
ILRTGGVLGVLVVQNRTERTYRDEEIETLQTIAMVLAEIVHRIEEDQSTPQPDRRFRGQS
HHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCC
SENLRGRIFCEGLGYGRAVLHDPVVPAARFFAADQKQETLRLTQGLKDLRTSIDRMMLID
CCCCCEEEEEECCCCCHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
GAALGEDPRDVMEAYRLLAHDNSWAEKLQEGVRSGLSAEASVDRARREHRAKMQAARDPY
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHH
LRERLHDLEDLDNRLLRILGGDENKPQITDESSVLVARRLGPAELLEYAKTGLKGILMEE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHCHHHHHHHH
AAASSHAAIVARALSIPAIGGIEGLTTLIEQGDYVIVDAEQGQAHIRPDDAVYDAYKSRV
HHCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHH
ALRSERQAVYASLKGLPAQTKDGETLSLMLNAGLDLDLDMLDQAGADGVGLFRTEFQFLV
HHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCHHHHHHCCCCCCHHHHHHHHHHH
SESLPRLEDQISLYRRVLDRAAGRPVIFRTLDLGGDKVLPILNQLREENPALGWRSIRFA
HHCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHH
LDHKGLFRRQLRALVRAAGEGPLTVMFPMVTTPEEFREARALLEEEVAWTLKRTGKTPSK
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LEIGVMLETPALAFSLSDLAGEVDFLSVGTNDLLQFFFAADRMTPSVSDRYALISPAALR
EEEEEEEECCHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCEEHCCHHHHH
FLRMVAETCKTAGIRLSVCGEATATPLAAFCFAAFGYSSLSMSASSIGPVKQALRSIDLA
HHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHCHHHHHCCHHCCCHHHHHHHHHHHH
GFRKGLDAALEKPNGAFRNQVLGIAIEHGADIPDT
HHHHHHHHHHCCCCCHHHHHHEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 7896715; 8973315 [H]