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The map label for this gene is iolD [H]

Identifier: 114798854

GI number: 114798854

Start: 2274466

End: 2276310

Strand: Direct

Name: iolD [H]

Synonym: HNE_2183

Alternate gene names: 114798854

Gene position: 2274466-2276310 (Clockwise)

Preceding gene: 114797675

Following gene: 114798293

Centisome position: 61.39

GC content: 58.86

Gene sequence:

>1845_bases
ATGGAAACGCTTAACCTGACAATGTCCCAGGCCCTGGTGCGCTATCTGGCCGCCCAGTATATGGTGATTGACGGGGAAGA
AGTTCCCTACTTCGCCGGCGTCTGGGCGATATTCGGGCATGGCAATGTCGCCGGTCTCGGTGAGGCGCTTTATACAGTGC
GAGACGAACTTCCAACCTACCGCGCGCACAATGAGCAAGGCATGGCGCACGCCGCGATTGCCTATGCGAAAGCAAGCTTT
CGCCGCCGCGCGATGGTCTGCACATCATCCATCGGCCCCGGCGCCACGAATTTCATCACGGCGGCCGCACTCGCGCATGT
GAACCGGCTGCCGGTGCTCTTCCTGCCTGGCGATATATTTGCAAACCGGCGGCCAGACCCTGTCCTTCAACAGATCGAAG
ATTTCCAGGACGGCACGGTGAGCGCCAATGACTGCTTCCGGCCGGTCTCTCGCTATTTTGACAGGATCACGCGTCCCGAA
CAAATCCTGACGGCCCTTCCCCGTATGATGTCAGTCTTCACCGATCCGGCTAGCTGTGGTCCGGTATGCCTGTCTCTTTG
CCAGGACGTGCAGGCCGAAGCGTATGACTACCCCGCCTCCTTCTTCTCCAGACGCGTCTGGTCCATACGGAAACCAAATC
CCGATATGGCCGAGCTGGAGGCTCTTATCGACGCCCTGAAAGCCGCCAGGCGGCCCCTGTTTGTTGCAGGCGGCGGCGCG
CTCTACAGTGGCGCCTCGCAAATCCTCAGTGAGCTGTCGGCCCGGCTCGGTATTCCAGTCGCCGAAACACAAGCGGGCCG
TAGCACACTTCCCGCCTCCCATCCCCAAAACCTAGGCGCTATCGGAGTGACCGGAACAGCGCCCGCGAATGCAGCTGCCG
CCGAAGCAGACCTTGTCCTCGGGATCGGAACGCGGCTGCAGGATTTCACCACAGGCTCCAGAACGCTGTTTTCCAGCCCC
GGCCGCAAACTCTTCCAGTTGAATACCGCGCCTTATGACGCCTCGAAACATGGTGCTGCTCCAATTGTTTGCGACGCCGC
CCGAGGCCTTACACTCTTGGCTGAAGCCCTCGAATCAACAAGCATTGCCGCAGACTGGATCACCCTAAATCAAGCGCGCA
ATCAAGATTGGCGCGCGGCGGTCCAATTGGCACTCACCGATATCAACCAACCAGTACCGAGCTATGCAGATGTGATCGGA
GCCGTAGACAGGTCGTCAGACGATCAGAGCATAGTCGTTTGCGCCGCAGGCACATTGCCCGGAGAGCTCCACAAGCTCTG
GCGAACCGACCGTATTGGCGGGTACCATATGGAGTATGGCTACTCCTGCATGGGTTATGAAATCGCTGGCGGCATGGGCG
TAAAAATGGCCTGCCCTGACAGCGAAGTATACGTGATGGTGGGTGATGGCAGCTATCTCATGCTCAATTCCGAGATCGCC
ACTTCGGTCATGATGGGCCTTAAACTCATCATTGTACTTCTGGATTCTGAAGGTTTCTCCTGCATCAACCGGCTCCAGCG
CTCGACGGGTAGCGCAGAGTTCAACAATCAGATGCGCGATACGGTTCACGACACGCTTCCGGTCATAGATTTTGCCGCCC
ACGCGGCGAGCCTTGGCGCAATTTCCGAACGGGTAACCAGCGTTGCCGAACTTGAAGCAGCGCTCGCCCGGGCGCGGTCA
GCCCCGGCCACAACCCTCATTTCCATTGCGGTCAATCCCGACGCAACGACAGATATGGGAGGCCATTGGTGGGATGTCGC
CGTCCCGGAAGTCTCGGGAAGCGCGGCTGTCCGTGAAGCCTTTGAAGCCTACCGCGCTGCCACACAAAAACAATTCGTAG
ACTGA

Upstream 100 bases:

>100_bases
CGATGGCGAAGCCGTTTTCGCCCTCCGCCGCAATTTCAGCCAGCTGATTGACGGTTGGCGTCAGTCCCGGCAGGCGCCCT
GAGCGAGAGGACACTGACGG

Downstream 100 bases:

>100_bases
CAGGAGTAACTCATGCGTTTCGGAGCAAGCCCGATTGCCTGGTCAAATGACGACATGCCAGAACTCGGCGCAGACACCTC
ACTGGACACCTGCCTGACAG

Product: thiamine pyrophosphate-dependent enzyme IolD

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase [H]

Number of amino acids: Translated: 614; Mature: 614

Protein sequence:

>614_residues
METLNLTMSQALVRYLAAQYMVIDGEEVPYFAGVWAIFGHGNVAGLGEALYTVRDELPTYRAHNEQGMAHAAIAYAKASF
RRRAMVCTSSIGPGATNFITAAALAHVNRLPVLFLPGDIFANRRPDPVLQQIEDFQDGTVSANDCFRPVSRYFDRITRPE
QILTALPRMMSVFTDPASCGPVCLSLCQDVQAEAYDYPASFFSRRVWSIRKPNPDMAELEALIDALKAARRPLFVAGGGA
LYSGASQILSELSARLGIPVAETQAGRSTLPASHPQNLGAIGVTGTAPANAAAAEADLVLGIGTRLQDFTTGSRTLFSSP
GRKLFQLNTAPYDASKHGAAPIVCDAARGLTLLAEALESTSIAADWITLNQARNQDWRAAVQLALTDINQPVPSYADVIG
AVDRSSDDQSIVVCAAGTLPGELHKLWRTDRIGGYHMEYGYSCMGYEIAGGMGVKMACPDSEVYVMVGDGSYLMLNSEIA
TSVMMGLKLIIVLLDSEGFSCINRLQRSTGSAEFNNQMRDTVHDTLPVIDFAAHAASLGAISERVTSVAELEAALARARS
APATTLISIAVNPDATTDMGGHWWDVAVPEVSGSAAVREAFEAYRAATQKQFVD

Sequences:

>Translated_614_residues
METLNLTMSQALVRYLAAQYMVIDGEEVPYFAGVWAIFGHGNVAGLGEALYTVRDELPTYRAHNEQGMAHAAIAYAKASF
RRRAMVCTSSIGPGATNFITAAALAHVNRLPVLFLPGDIFANRRPDPVLQQIEDFQDGTVSANDCFRPVSRYFDRITRPE
QILTALPRMMSVFTDPASCGPVCLSLCQDVQAEAYDYPASFFSRRVWSIRKPNPDMAELEALIDALKAARRPLFVAGGGA
LYSGASQILSELSARLGIPVAETQAGRSTLPASHPQNLGAIGVTGTAPANAAAAEADLVLGIGTRLQDFTTGSRTLFSSP
GRKLFQLNTAPYDASKHGAAPIVCDAARGLTLLAEALESTSIAADWITLNQARNQDWRAAVQLALTDINQPVPSYADVIG
AVDRSSDDQSIVVCAAGTLPGELHKLWRTDRIGGYHMEYGYSCMGYEIAGGMGVKMACPDSEVYVMVGDGSYLMLNSEIA
TSVMMGLKLIIVLLDSEGFSCINRLQRSTGSAEFNNQMRDTVHDTLPVIDFAAHAASLGAISERVTSVAELEAALARARS
APATTLISIAVNPDATTDMGGHWWDVAVPEVSGSAAVREAFEAYRAATQKQFVD
>Mature_614_residues
METLNLTMSQALVRYLAAQYMVIDGEEVPYFAGVWAIFGHGNVAGLGEALYTVRDELPTYRAHNEQGMAHAAIAYAKASF
RRRAMVCTSSIGPGATNFITAAALAHVNRLPVLFLPGDIFANRRPDPVLQQIEDFQDGTVSANDCFRPVSRYFDRITRPE
QILTALPRMMSVFTDPASCGPVCLSLCQDVQAEAYDYPASFFSRRVWSIRKPNPDMAELEALIDALKAARRPLFVAGGGA
LYSGASQILSELSARLGIPVAETQAGRSTLPASHPQNLGAIGVTGTAPANAAAAEADLVLGIGTRLQDFTTGSRTLFSSP
GRKLFQLNTAPYDASKHGAAPIVCDAARGLTLLAEALESTSIAADWITLNQARNQDWRAAVQLALTDINQPVPSYADVIG
AVDRSSDDQSIVVCAAGTLPGELHKLWRTDRIGGYHMEYGYSCMGYEIAGGMGVKMACPDSEVYVMVGDGSYLMLNSEIA
TSVMMGLKLIIVLLDSEGFSCINRLQRSTGSAEFNNQMRDTVHDTLPVIDFAAHAASLGAISERVTSVAELEAALARARS
APATTLISIAVNPDATTDMGGHWWDVAVPEVSGSAAVREAFEAYRAATQKQFVD

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081685, Length=540, Percent_Identity=27.2222222222222, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1790104, Length=530, Percent_Identity=24.3396226415094, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1786717, Length=464, Percent_Identity=25.4310344827586, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI1788716, Length=529, Percent_Identity=23.8185255198488, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17531299, Length=518, Percent_Identity=22.5868725868726, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17531301, Length=518, Percent_Identity=22.5868725868726, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323755, Length=552, Percent_Identity=24.6376811594203, Blast_Score=118, Evalue=3e-27,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 65775; Mature: 65775

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METLNLTMSQALVRYLAAQYMVIDGEEVPYFAGVWAIFGHGNVAGLGEALYTVRDELPTY
CCCCHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHEECCCCHHHHHHHHHHHHHHCCCC
RAHNEQGMAHAAIAYAKASFRRRAMVCTSSIGPGATNFITAAALAHVNRLPVLFLPGDIF
CCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHCCCCEEEECCHHH
ANRRPDPVLQQIEDFQDGTVSANDCFRPVSRYFDRITRPEQILTALPRMMSVFTDPASCG
CCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHH
PVCLSLCQDVQAEAYDYPASFFSRRVWSIRKPNPDMAELEALIDALKAARRPLFVAGGGA
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCH
LYSGASQILSELSARLGIPVAETQAGRSTLPASHPQNLGAIGVTGTAPANAAAAEADLVL
HHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCHHCCCCEEE
GIGTRLQDFTTGSRTLFSSPGRKLFQLNTAPYDASKHGAAPIVCDAARGLTLLAEALEST
ECCCHHHHCCCCCCHHHCCCCCEEEEECCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHC
SIAADWITLNQARNQDWRAAVQLALTDINQPVPSYADVIGAVDRSSDDQSIVVCAAGTLP
CCEEHHEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCC
GELHKLWRTDRIGGYHMEYGYSCMGYEIAGGMGVKMACPDSEVYVMVGDGSYLMLNSEIA
HHHHHHHHHCCCCCEEECCCCEEECEEECCCCEEEEECCCCCEEEEECCCCEEEECHHHH
TSVMMGLKLIIVLLDSEGFSCINRLQRSTGSAEFNNQMRDTVHDTLPVIDFAAHAASLGA
HHHHHHHHHEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISERVTSVAELEAALARARSAPATTLISIAVNPDATTDMGGHWWDVAVPEVSGSAAVREA
HHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCEEEEECCCCCCHHHHHHH
FEAYRAATQKQFVD
HHHHHHHHHHHCCC
>Mature Secondary Structure
METLNLTMSQALVRYLAAQYMVIDGEEVPYFAGVWAIFGHGNVAGLGEALYTVRDELPTY
CCCCHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHEECCCCHHHHHHHHHHHHHHCCCC
RAHNEQGMAHAAIAYAKASFRRRAMVCTSSIGPGATNFITAAALAHVNRLPVLFLPGDIF
CCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHCCCCEEEECCHHH
ANRRPDPVLQQIEDFQDGTVSANDCFRPVSRYFDRITRPEQILTALPRMMSVFTDPASCG
CCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHH
PVCLSLCQDVQAEAYDYPASFFSRRVWSIRKPNPDMAELEALIDALKAARRPLFVAGGGA
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCH
LYSGASQILSELSARLGIPVAETQAGRSTLPASHPQNLGAIGVTGTAPANAAAAEADLVL
HHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCHHCCCCEEE
GIGTRLQDFTTGSRTLFSSPGRKLFQLNTAPYDASKHGAAPIVCDAARGLTLLAEALEST
ECCCHHHHCCCCCCHHHCCCCCEEEEECCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHC
SIAADWITLNQARNQDWRAAVQLALTDINQPVPSYADVIGAVDRSSDDQSIVVCAAGTLP
CCEEHHEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCC
GELHKLWRTDRIGGYHMEYGYSCMGYEIAGGMGVKMACPDSEVYVMVGDGSYLMLNSEIA
HHHHHHHHHCCCCCEEECCCCEEECEEECCCCEEEEECCCCCEEEEECCCCEEEECHHHH
TSVMMGLKLIIVLLDSEGFSCINRLQRSTGSAEFNNQMRDTVHDTLPVIDFAAHAASLGA
HHHHHHHHHEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISERVTSVAELEAALARARSAPATTLISIAVNPDATTDMGGHWWDVAVPEVSGSAAVREA
HHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCEEEEECCCCCCHHHHHHH
FEAYRAATQKQFVD
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2 pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; Decarboxylation [C]

Inhibitor: 2-oxobutanoate; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA