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The map label for this gene is nac [H]

Identifier: 114798660

GI number: 114798660

Start: 2316492

End: 2317430

Strand: Direct

Name: nac [H]

Synonym: HNE_2216

Alternate gene names: 114798660

Gene position: 2316492-2317430 (Clockwise)

Preceding gene: 114800456

Following gene: 114799609

Centisome position: 62.52

GC content: 62.62

Gene sequence:

>939_bases
ATGAAACTGCGCCAGCTGGCGGCCTTCATCCGTGTATGCCAGATCGGAAGCATCACCCGCGCAGCGGCAGAGCTGAACAT
TGCCCAGCCCGCACTGGGTTTGCAGATCCGCAATCTGGAATCTGATTTCGGCGCCGAGCTGATTGCCCGCGGGCCAAGGG
GCGTACGCCCCACCCCGGCAGGAGAACTCGTTCTGGCCTGGGCGCAGGATGTTCTGCAAAGCCGCGACAGCATTCTTCAC
AAGGTGCGCGAGCTCGCGGCCAGCGCGCCACCCAGTCTGAAACTCGGTCTGACGCCGAGCGTGGCAGGTCTGATCTCTGA
CAGCCTTGTGGACTGGGCCAATACAGAGGGGCGCTCGGTCAGCCTGTCCCTGATCAAAGCGTTCAGCAACAACATCTCTC
AGCTTGTCGAGATGCAGCAACTGGACCTTGGCCTGACCTGCAGCGCACGCCCCCGCGAAGCGCTCGATAGCCAGCCGATC
CTGCTCGAGCGGCTGTACTTTATGGGCGCGTCCGACGGGACTACAGGGCCCATCTCCCTTGAGGACGCGCTTGACCGGCC
CATCGCCCTTCCGAAAGGACGTGACTCGGTGCGCCGCGCCGTCGATGAAGGCGTGCTCCGTCAAGGGCTGGTTCTGAAAA
ACACGATGGAGATCAACTCGCTGGAAACCGCCAAAGATCTCGCGGTTGCCGGGATTGCCGGATGCATCCTTCCGCTGGGA
AGCGCGCCGGATGCGGCCAGCCTGGGCCTCAGGCTGCGCCCGATTATTTCCCCCGCCCTGACCCGGACGATCTATCTCGT
CCGCCGGTACGACCATATTCCCACCGATGCGGAACAAAGGCTGATTAACACCATGACAGCCACCCTGCTCGGCCTGATCG
GCGAAGCCGGGATTAGCCCGTATGAACGCCCGACGGAGACGGTCTCTGAAAAGGGATGA

Upstream 100 bases:

>100_bases
AGCGCTCTGCGTGCTTCAGGCGCCGTCGCCGGTTTTGCGCGTGCCGGCTTATGCGGCAGAGAGAAGGTGTGGCGCAGATG
CCGAACCAGGGAGAACGGGA

Downstream 100 bases:

>100_bases
TTCTCCCGGCACGGCGGACAGGCGTGTCCTCCGCCCCCGTATCATGTGTCGGGGAGCCCGGAGCCTCACGTCAGTGCTCG
CTGCCAACCGGGTTCGACAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Nitrogen assimilation control protein [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MKLRQLAAFIRVCQIGSITRAAAELNIAQPALGLQIRNLESDFGAELIARGPRGVRPTPAGELVLAWAQDVLQSRDSILH
KVRELAASAPPSLKLGLTPSVAGLISDSLVDWANTEGRSVSLSLIKAFSNNISQLVEMQQLDLGLTCSARPREALDSQPI
LLERLYFMGASDGTTGPISLEDALDRPIALPKGRDSVRRAVDEGVLRQGLVLKNTMEINSLETAKDLAVAGIAGCILPLG
SAPDAASLGLRLRPIISPALTRTIYLVRRYDHIPTDAEQRLINTMTATLLGLIGEAGISPYERPTETVSEKG

Sequences:

>Translated_312_residues
MKLRQLAAFIRVCQIGSITRAAAELNIAQPALGLQIRNLESDFGAELIARGPRGVRPTPAGELVLAWAQDVLQSRDSILH
KVRELAASAPPSLKLGLTPSVAGLISDSLVDWANTEGRSVSLSLIKAFSNNISQLVEMQQLDLGLTCSARPREALDSQPI
LLERLYFMGASDGTTGPISLEDALDRPIALPKGRDSVRRAVDEGVLRQGLVLKNTMEINSLETAKDLAVAGIAGCILPLG
SAPDAASLGLRLRPIISPALTRTIYLVRRYDHIPTDAEQRLINTMTATLLGLIGEAGISPYERPTETVSEKG
>Mature_312_residues
MKLRQLAAFIRVCQIGSITRAAAELNIAQPALGLQIRNLESDFGAELIARGPRGVRPTPAGELVLAWAQDVLQSRDSILH
KVRELAASAPPSLKLGLTPSVAGLISDSLVDWANTEGRSVSLSLIKAFSNNISQLVEMQQLDLGLTCSARPREALDSQPI
LLERLYFMGASDGTTGPISLEDALDRPIALPKGRDSVRRAVDEGVLRQGLVLKNTMEINSLETAKDLAVAGIAGCILPLG
SAPDAASLGLRLRPIISPALTRTIYLVRRYDHIPTDAEQRLINTMTATLLGLIGEAGISPYERPTETVSEKG

Specific function: Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=243, Percent_Identity=27.1604938271605, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI157672245, Length=286, Percent_Identity=26.5734265734266, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI145693105, Length=295, Percent_Identity=22.7118644067797, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789506, Length=242, Percent_Identity=25.2066115702479, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790399, Length=273, Percent_Identity=23.0769230769231, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787879, Length=178, Percent_Identity=31.4606741573034, Blast_Score=62, Evalue=7e-11,
Organism=Escherichia coli, GI1788887, Length=108, Percent_Identity=37.037037037037, Blast_Score=61, Evalue=7e-11,
Organism=Escherichia coli, GI1787601, Length=208, Percent_Identity=24.0384615384615, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33410; Mature: 33410

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRQLAAFIRVCQIGSITRAAAELNIAQPALGLQIRNLESDFGAELIARGPRGVRPTPA
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHCCEEECCCHHHHHHHHHCCCCCCCCCCC
GELVLAWAQDVLQSRDSILHKVRELAASAPPSLKLGLTPSVAGLISDSLVDWANTEGRSV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCH
SLSLIKAFSNNISQLVEMQQLDLGLTCSARPREALDSQPILLERLYFMGASDGTTGPISL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCHHHHHHHHHCCCCCCCCCEEH
EDALDRPIALPKGRDSVRRAVDEGVLRQGLVLKNTMEINSLETAKDLAVAGIAGCILPLG
HHHCCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
SAPDAASLGLRLRPIISPALTRTIYLVRRYDHIPTDAEQRLINTMTATLLGLIGEAGISP
CCCCHHHCCCEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
YERPTETVSEKG
CCCCHHHHCCCC
>Mature Secondary Structure
MKLRQLAAFIRVCQIGSITRAAAELNIAQPALGLQIRNLESDFGAELIARGPRGVRPTPA
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHCCEEECCCHHHHHHHHHCCCCCCCCCCC
GELVLAWAQDVLQSRDSILHKVRELAASAPPSLKLGLTPSVAGLISDSLVDWANTEGRSV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCH
SLSLIKAFSNNISQLVEMQQLDLGLTCSARPREALDSQPILLERLYFMGASDGTTGPISL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCHHHHHHHHHCCCCCCCCCEEH
EDALDRPIALPKGRDSVRRAVDEGVLRQGLVLKNTMEINSLETAKDLAVAGIAGCILPLG
HHHCCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
SAPDAASLGLRLRPIISPALTRTIYLVRRYDHIPTDAEQRLINTMTATLLGLIGEAGISP
CCCCHHHCCCEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
YERPTETVSEKG
CCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8458853 [H]