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The map label for this gene is tag [H]

Identifier: 114798473

GI number: 114798473

Start: 877802

End: 878383

Strand: Reverse

Name: tag [H]

Synonym: HNE_0860

Alternate gene names: 114798473

Gene position: 878383-877802 (Counterclockwise)

Preceding gene: 114798165

Following gene: 114797522

Centisome position: 23.71

GC content: 59.79

Gene sequence:

>582_bases
ATGAGTGAACCCCATTCCTGCGGCTGGGCGCCGGTGACCGATCCCATTTACCGCGCCTATCATGATGAAGAATGGGGCGT
GCCCGAGCGCGACCCGCGCGCGCTGTGGGAGAAGCTGCAGCTGGACGGGATGCAGGCAGGGCTGAGCTGGATCACAATTC
TGAGGAAGCGGGAGAGCATTCGGGAAGAGTTTGACGAGTTTGACCCGGAAAAATTGGCTCGCTGGACGCCCAGACGCGCT
GAAAAGGCGCTGACCAATCCCGGCATCATCCGCAGCCCCAAGAAGATCGACGCCGTGATCGGCAACGCCCGGAGCTACCT
GTCCATGATGGAAGCGGGCGATGATTTCTCGGATTACTGCTGGGGCTATGTCGGCGGCAAACCCGTTGTAAACAAATGGA
AGAATTTCCGCGAGGCGCCCACCAGCACGGACTGGTCGGCGGCAATGTCCAGGGACCTCAAGAAGCGCGGCTTCAAATTT
GTAGGCCCGACAATTGTCTATGCGTGGGCACAGGCCGTTGGCATGGTCAACGATCATGAGGTAGGGTGTCCCCGTCACAA
GGGTGTGCGGGAGAGACCATGA

Upstream 100 bases:

>100_bases
CAGGATAAACAGCCTGTCACCGTGAACGACGCTCCCGCCGAAATCGACGGCGCGCCGTGGCTGATTGCCGAAGTCAAAGC
CCATCTGGAGGCCAAAGCCG

Downstream 100 bases:

>100_bases
AAACGCTTATGTTTATGACGGCGATGGGATTGCTGGCGGCTGTCGGCGGCTGCGCAAGCGGTACCCCGTCGAACGCGTAT
GCCAACCCGGTGGATGAATG

Product: DNA-3-methyladenine glycosylase I

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosylase I, constitutive; TAG I; DNA-3-methyladenine glycosidase I; DNA-3-methyladenine glycosylase I [H]

Number of amino acids: Translated: 193; Mature: 192

Protein sequence:

>193_residues
MSEPHSCGWAPVTDPIYRAYHDEEWGVPERDPRALWEKLQLDGMQAGLSWITILRKRESIREEFDEFDPEKLARWTPRRA
EKALTNPGIIRSPKKIDAVIGNARSYLSMMEAGDDFSDYCWGYVGGKPVVNKWKNFREAPTSTDWSAAMSRDLKKRGFKF
VGPTIVYAWAQAVGMVNDHEVGCPRHKGVRERP

Sequences:

>Translated_193_residues
MSEPHSCGWAPVTDPIYRAYHDEEWGVPERDPRALWEKLQLDGMQAGLSWITILRKRESIREEFDEFDPEKLARWTPRRA
EKALTNPGIIRSPKKIDAVIGNARSYLSMMEAGDDFSDYCWGYVGGKPVVNKWKNFREAPTSTDWSAAMSRDLKKRGFKF
VGPTIVYAWAQAVGMVNDHEVGCPRHKGVRERP
>Mature_192_residues
SEPHSCGWAPVTDPIYRAYHDEEWGVPERDPRALWEKLQLDGMQAGLSWITILRKRESIREEFDEFDPEKLARWTPRRAE
KALTNPGIIRSPKKIDAVIGNARSYLSMMEAGDDFSDYCWGYVGGKPVVNKWKNFREAPTSTDWSAAMSRDLKKRGFKFV
GPTIVYAWAQAVGMVNDHEVGCPRHKGVRERP

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG2818

COG function: function code L; 3-methyladenine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789971, Length=182, Percent_Identity=52.1978021978022, Blast_Score=201, Evalue=4e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005019
- InterPro:   IPR011257
- InterPro:   IPR004597 [H]

Pfam domain/function: PF03352 Adenine_glyco [H]

EC number: =3.2.2.20 [H]

Molecular weight: Translated: 22155; Mature: 22024

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPHSCGWAPVTDPIYRAYHDEEWGVPERDPRALWEKLQLDGMQAGLSWITILRKRESI
CCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REEFDEFDPEKLARWTPRRAEKALTNPGIIRSPKKIDAVIGNARSYLSMMEAGDDFSDYC
HHHHHHCCHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
WGYVGGKPVVNKWKNFREAPTSTDWSAAMSRDLKKRGFKFVGPTIVYAWAQAVGMVNDHE
HHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCC
VGCPRHKGVRERP
CCCCCCCCCCCCC
>Mature Secondary Structure 
SEPHSCGWAPVTDPIYRAYHDEEWGVPERDPRALWEKLQLDGMQAGLSWITILRKRESI
CCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REEFDEFDPEKLARWTPRRAEKALTNPGIIRSPKKIDAVIGNARSYLSMMEAGDDFSDYC
HHHHHHCCHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
WGYVGGKPVVNKWKNFREAPTSTDWSAAMSRDLKKRGFKFVGPTIVYAWAQAVGMVNDHE
HHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCC
VGCPRHKGVRERP
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536912; 3520491; 8041620; 9278503 [H]