Click here to switch to the map view.

The map label for this gene is pheT [H]

Identifier: 114798222

GI number: 114798222

Start: 431218

End: 433611

Strand: Direct

Name: pheT [H]

Synonym: HNE_0435

Alternate gene names: 114798222

Gene position: 431218-433611 (Clockwise)

Preceding gene: 114797877

Following gene: 114797481

Centisome position: 11.64

GC content: 62.95

Gene sequence:

>2394_bases
ATGAAATTCTCCCTTTCCTGGCTGAACGATCACCTTGTTACCAAGGCATCGCTTCAGGACATTCTCGATCTGATGCTCAA
GGCCGGCCTTGAGGTTGAAGAGGTGACTGACCCGCGCGAGAAACTTGCGCCGTTCACCGTCTGCAAGGTGATTGAGGCCA
AGCCCCATCCGGATGCGGACAAATTGCGCGTCTGCACGGTAGAAACCATTGACGGCGTCAAGCAGATCGTCTGCGGCGCC
CCCAATGCGCGCACCGGCATGACCGCGATCTATGCCCCGCTCGGCACCTTCATTCCGGGGCTCGATTTCGCGCTCGACAA
GAAGCCCCGCGCCATTCGCGGGATTGAGAGCCATGGCATGATGTGCTCCTCGCGCGAGCTTGAGGTGGGTGAGGACCATG
ACGGCATTCTCGATCTTGATGACAGCCTGAAGGTCGGCACGCCCGCCGCCGAGGCACTTGGCCAGACCGACCCCGTGATC
GACTTTGAAGTGACGCCTAACCGGCCCGACTGGCTGGGCGTGCAGGGTATTGCGCGCGACCTCGCTGCGGCCGGGGCAGG
GCGTTTCCTGCGTAACGATCCCAAGAAGGTCAATGGCAGCTATGACTGCCCCATCGACATCAAGCTTGAGGCCACAGAGG
CCTGTCCGATGTTTGCCGGGGCGCTGATCCGCGGCGTCACCAATGGTCCGTCACCGGACTGGATGCAGGCGCGCCTCAAG
GCAGCCGGTCTCCAGCCCAAATCGCTGCTCGTTGATGTGACGAACTATATCTCTCTTGACCGCGCGCGCCCGCTGCACGT
GTATGATGCAGCCAGGCTCTCCGGCGCCGTGGTTGCCCGGCTCGGCAGGAAGGGCGAGACGCTCGAGGCGCTCGATGGGA
AGACCTATCCGATCACGGAAGACATGTGCGTCATCGCCGACAATTCCGGCGCGATCGGTCTGGGCGGCGTCATGGGCGGC
GTTTCGACAGCTGTTTCGGCGGAGACCGTGGACGTCTTCATTGAATCTGCCTGGTTCGATCCGTTGCGCACCGCGCGCAC
GGGCCGCGCCACGGGCATCCATTCGGACGCCCGCTACCGGTTCGAGCGCGGCGTTGATCCGCACAGCTCAATCGATGGCC
TCAACTTCGCGATTGCGCTGATCCTGGAATATGGCGGCGGTCAGGTCTCGAAGGCAAAGGTCGCCGGATCTATCCCGGCG
CGAATGCAGAAGGTCACCTTCTACCCCGTTGATGTCGAGCGCCTCACGGGCCTCATCGTCAAGCCGGCGGAAATGAAGCG
CATCCTCAAGGATCTCGAATTCGACATCGAGGATGCAGGTGATGCCTGGTATCTCCTGCCCCCCTCGCACCGGTTCGACA
TGGAACAGTCGGCTGACATCGTAGAAGAAATTGCCCGCCTGATCGGCTTTGACCAGTTGCCGACCACCTCCCTGCCGCTG
CCTGAAGGCGGGCGCCGGGTGATCACGACGCCGCGTCAGGCGCGAGTGGCCATCTCGCGCCGTGTCATGGCGGCGCGCGG
TTTCCTTGAAGCGGTCACCTGGAGCTTCATGGCGCGGGAACATGCCGACCTGTTCGGCAAGACCGAGGATGTGCTCGCCG
TGGCAAACCCTGTCGCCAGCGAGTTGAACTACATGCGCCCCTCGGCATTGGGCAACCTTGCCCAGGCGGCCCAGCGCGCG
CGCAATCGCGGTGAGCGCACCATCCGCCTCTTTGAAGCTGGCCCCATCTATCTCGGCGATGGCCCCAAAGATCAGCGTAC
TGTGATTGCCGGTCTCGTTCTGCCTGCGGCTGAGCGCCACTGGCAGGGCCGCGCTGCGCCTTATGACGCCTATGCCGCCA
AGGCCGATCTCTTCGCGCTGCTTGCTGCGCTCGGCCAGCCGGGCGAGCGTTTCCAGGTCGCGGCGCCAACCCAGCCCCAC
TGGCATCCCGGCCAGGCCGCCAGCCTCAAGCTCGGCCCGAAGATGACCGTTGCCAATTTCGGCGCGCTGCATCCCGGCGT
GCTCAAGACGCTGGATGTTGAGGGCCCGGTCTATGCGTTCGAGTTGAACCTCAACGCCCTGCCGGTGATGAAGGTGAAAT
CCACCAAGACCAAACCGGTTCTGGAACGTGCTGAGCTGACACCGATCCGCCGCGACCTCGCTTTTGTGGTCGATGCGGGC
GTGGCCGCCGGAGATGTCGTCCGTCATGCTCAGTCTGCCGAGAAGCAGCTCATCGCAGCAATCGACGTGTTCGATGTGTA
TCAGGGCCAGGGCGTCGGCGAGGGGAAGAAGTCGGTGGCGATCGAAATCACGCTCCAGCCAAAAGAGGCCCTTAAGGACG
AGCAGATCCAACAGGTAATCGAGCGTATCGTCGCTTCGGTCGCCAAGGGAACCGGCGGTGTGCTAAGAGGCTAA

Upstream 100 bases:

>100_bases
CCATTCTCGATGACGACTTCGCCGAAGGGGCGGAAGGCGAAGTCAAAGCCGAGCGCGCAGAGGCGCATGCGGCAGGTTAC
ACGGCTCTTGGAGCGGATAC

Downstream 100 bases:

>100_bases
CGGGAACAGCAAGGGACGGCTGGGATGGGACTGAAACGGCGGCTTTATGATGCGTTAGCGCCAGAGGCGCGCCGGTCTGG
TCTCTCGATGACGAATGCGG

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 797; Mature: 797

Protein sequence:

>797_residues
MKFSLSWLNDHLVTKASLQDILDLMLKAGLEVEEVTDPREKLAPFTVCKVIEAKPHPDADKLRVCTVETIDGVKQIVCGA
PNARTGMTAIYAPLGTFIPGLDFALDKKPRAIRGIESHGMMCSSRELEVGEDHDGILDLDDSLKVGTPAAEALGQTDPVI
DFEVTPNRPDWLGVQGIARDLAAAGAGRFLRNDPKKVNGSYDCPIDIKLEATEACPMFAGALIRGVTNGPSPDWMQARLK
AAGLQPKSLLVDVTNYISLDRARPLHVYDAARLSGAVVARLGRKGETLEALDGKTYPITEDMCVIADNSGAIGLGGVMGG
VSTAVSAETVDVFIESAWFDPLRTARTGRATGIHSDARYRFERGVDPHSSIDGLNFAIALILEYGGGQVSKAKVAGSIPA
RMQKVTFYPVDVERLTGLIVKPAEMKRILKDLEFDIEDAGDAWYLLPPSHRFDMEQSADIVEEIARLIGFDQLPTTSLPL
PEGGRRVITTPRQARVAISRRVMAARGFLEAVTWSFMAREHADLFGKTEDVLAVANPVASELNYMRPSALGNLAQAAQRA
RNRGERTIRLFEAGPIYLGDGPKDQRTVIAGLVLPAAERHWQGRAAPYDAYAAKADLFALLAALGQPGERFQVAAPTQPH
WHPGQAASLKLGPKMTVANFGALHPGVLKTLDVEGPVYAFELNLNALPVMKVKSTKTKPVLERAELTPIRRDLAFVVDAG
VAAGDVVRHAQSAEKQLIAAIDVFDVYQGQGVGEGKKSVAIEITLQPKEALKDEQIQQVIERIVASVAKGTGGVLRG

Sequences:

>Translated_797_residues
MKFSLSWLNDHLVTKASLQDILDLMLKAGLEVEEVTDPREKLAPFTVCKVIEAKPHPDADKLRVCTVETIDGVKQIVCGA
PNARTGMTAIYAPLGTFIPGLDFALDKKPRAIRGIESHGMMCSSRELEVGEDHDGILDLDDSLKVGTPAAEALGQTDPVI
DFEVTPNRPDWLGVQGIARDLAAAGAGRFLRNDPKKVNGSYDCPIDIKLEATEACPMFAGALIRGVTNGPSPDWMQARLK
AAGLQPKSLLVDVTNYISLDRARPLHVYDAARLSGAVVARLGRKGETLEALDGKTYPITEDMCVIADNSGAIGLGGVMGG
VSTAVSAETVDVFIESAWFDPLRTARTGRATGIHSDARYRFERGVDPHSSIDGLNFAIALILEYGGGQVSKAKVAGSIPA
RMQKVTFYPVDVERLTGLIVKPAEMKRILKDLEFDIEDAGDAWYLLPPSHRFDMEQSADIVEEIARLIGFDQLPTTSLPL
PEGGRRVITTPRQARVAISRRVMAARGFLEAVTWSFMAREHADLFGKTEDVLAVANPVASELNYMRPSALGNLAQAAQRA
RNRGERTIRLFEAGPIYLGDGPKDQRTVIAGLVLPAAERHWQGRAAPYDAYAAKADLFALLAALGQPGERFQVAAPTQPH
WHPGQAASLKLGPKMTVANFGALHPGVLKTLDVEGPVYAFELNLNALPVMKVKSTKTKPVLERAELTPIRRDLAFVVDAG
VAAGDVVRHAQSAEKQLIAAIDVFDVYQGQGVGEGKKSVAIEITLQPKEALKDEQIQQVIERIVASVAKGTGGVLRG
>Mature_797_residues
MKFSLSWLNDHLVTKASLQDILDLMLKAGLEVEEVTDPREKLAPFTVCKVIEAKPHPDADKLRVCTVETIDGVKQIVCGA
PNARTGMTAIYAPLGTFIPGLDFALDKKPRAIRGIESHGMMCSSRELEVGEDHDGILDLDDSLKVGTPAAEALGQTDPVI
DFEVTPNRPDWLGVQGIARDLAAAGAGRFLRNDPKKVNGSYDCPIDIKLEATEACPMFAGALIRGVTNGPSPDWMQARLK
AAGLQPKSLLVDVTNYISLDRARPLHVYDAARLSGAVVARLGRKGETLEALDGKTYPITEDMCVIADNSGAIGLGGVMGG
VSTAVSAETVDVFIESAWFDPLRTARTGRATGIHSDARYRFERGVDPHSSIDGLNFAIALILEYGGGQVSKAKVAGSIPA
RMQKVTFYPVDVERLTGLIVKPAEMKRILKDLEFDIEDAGDAWYLLPPSHRFDMEQSADIVEEIARLIGFDQLPTTSLPL
PEGGRRVITTPRQARVAISRRVMAARGFLEAVTWSFMAREHADLFGKTEDVLAVANPVASELNYMRPSALGNLAQAAQRA
RNRGERTIRLFEAGPIYLGDGPKDQRTVIAGLVLPAAERHWQGRAAPYDAYAAKADLFALLAALGQPGERFQVAAPTQPH
WHPGQAASLKLGPKMTVANFGALHPGVLKTLDVEGPVYAFELNLNALPVMKVKSTKTKPVLERAELTPIRRDLAFVVDAG
VAAGDVVRHAQSAEKQLIAAIDVFDVYQGQGVGEGKKSVAIEITLQPKEALKDEQIQQVIERIVASVAKGTGGVLRG

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=809, Percent_Identity=32.5092707045735, Blast_Score=411, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17533403, Length=577, Percent_Identity=22.3570190641248, Blast_Score=71, Evalue=3e-12,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 85958; Mature: 85958

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS50886 TRBD ; PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSLSWLNDHLVTKASLQDILDLMLKAGLEVEEVTDPREKLAPFTVCKVIEAKPHPDAD
CEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHCHHHHHHHHCCCCCCCCC
KLRVCTVETIDGVKQIVCGAPNARTGMTAIYAPLGTFIPGLDFALDKKPRAIRGIESHGM
CEEEEEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHCCCCCHHHHCHHHCCC
MCSSRELEVGEDHDGILDLDDSLKVGTPAAEALGQTDPVIDFEVTPNRPDWLGVQGIARD
EECCCCCCCCCCCCCEEECCCCCCCCCCHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHH
LAAAGAGRFLRNDPKKVNGSYDCPIDIKLEATEACPMFAGALIRGVTNGPSPDWMQARLK
HHHHCCCHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHH
AAGLQPKSLLVDVTNYISLDRARPLHVYDAARLSGAVVARLGRKGETLEALDGKTYPITE
HCCCCCHHHHEEHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCC
DMCVIADNSGAIGLGGVMGGVSTAVSAETVDVFIESAWFDPLRTARTGRATGIHSDARYR
CEEEEECCCCCEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCHHHHH
FERGVDPHSSIDGLNFAIALILEYGGGQVSKAKVAGSIPARMQKVTFYPVDVERLTGLIV
HHCCCCCCCCCCCHHEEEEEEEECCCCCEEHHHHCCCCCCHHEEEEEEECCHHHHCCEEE
KPAEMKRILKDLEFDIEDAGDAWYLLPPSHRFDMEQSADIVEEIARLIGFDQLPTTSLPL
CHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
PEGGRRVITTPRQARVAISRRVMAARGFLEAVTWSFMAREHADLFGKTEDVLAVANPVAS
CCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
ELNYMRPSALGNLAQAAQRARNRGERTIRLFEAGPIYLGDGPKDQRTVIAGLVLPAAERH
HHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEECCCCCCCCEEEHHHHHHHHHHC
WQGRAAPYDAYAAKADLFALLAALGQPGERFQVAAPTQPHWHPGQAASLKLGPKMTVANF
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCEEECCCCEEHHHC
GALHPGVLKTLDVEGPVYAFELNLNALPVMKVKSTKTKPVLERAELTPIRRDLAFVVDAG
CCCCCCHHEEEECCCCEEEEEECCCCCEEEEECCCCCCHHHHHHHCCHHHHHHHHHHHCC
VAAGDVVRHAQSAEKQLIAAIDVFDVYQGQGVGEGKKSVAIEITLQPKEALKDEQIQQVI
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCHHHHCHHHHHHHH
ERIVASVAKGTGGVLRG
HHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKFSLSWLNDHLVTKASLQDILDLMLKAGLEVEEVTDPREKLAPFTVCKVIEAKPHPDAD
CEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHCHHHHHHHHCCCCCCCCC
KLRVCTVETIDGVKQIVCGAPNARTGMTAIYAPLGTFIPGLDFALDKKPRAIRGIESHGM
CEEEEEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHCCCCCHHHHCHHHCCC
MCSSRELEVGEDHDGILDLDDSLKVGTPAAEALGQTDPVIDFEVTPNRPDWLGVQGIARD
EECCCCCCCCCCCCCEEECCCCCCCCCCHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHH
LAAAGAGRFLRNDPKKVNGSYDCPIDIKLEATEACPMFAGALIRGVTNGPSPDWMQARLK
HHHHCCCHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHH
AAGLQPKSLLVDVTNYISLDRARPLHVYDAARLSGAVVARLGRKGETLEALDGKTYPITE
HCCCCCHHHHEEHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCC
DMCVIADNSGAIGLGGVMGGVSTAVSAETVDVFIESAWFDPLRTARTGRATGIHSDARYR
CEEEEECCCCCEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCHHHHH
FERGVDPHSSIDGLNFAIALILEYGGGQVSKAKVAGSIPARMQKVTFYPVDVERLTGLIV
HHCCCCCCCCCCCHHEEEEEEEECCCCCEEHHHHCCCCCCHHEEEEEEECCHHHHCCEEE
KPAEMKRILKDLEFDIEDAGDAWYLLPPSHRFDMEQSADIVEEIARLIGFDQLPTTSLPL
CHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
PEGGRRVITTPRQARVAISRRVMAARGFLEAVTWSFMAREHADLFGKTEDVLAVANPVAS
CCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
ELNYMRPSALGNLAQAAQRARNRGERTIRLFEAGPIYLGDGPKDQRTVIAGLVLPAAERH
HHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEECCCCCCCCEEEHHHHHHHHHHC
WQGRAAPYDAYAAKADLFALLAALGQPGERFQVAAPTQPHWHPGQAASLKLGPKMTVANF
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCEEECCCCEEHHHC
GALHPGVLKTLDVEGPVYAFELNLNALPVMKVKSTKTKPVLERAELTPIRRDLAFVVDAG
CCCCCCHHEEEECCCCEEEEEECCCCCEEEEECCCCCCHHHHHHHCCHHHHHHHHHHHCC
VAAGDVVRHAQSAEKQLIAAIDVFDVYQGQGVGEGKKSVAIEITLQPKEALKDEQIQQVI
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCHHHHCHHHHHHHH
ERIVASVAKGTGGVLRG
HHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]