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The map label for this gene is thyA

Identifier: 114798205

GI number: 114798205

Start: 454360

End: 455154

Strand: Direct

Name: thyA

Synonym: HNE_0459

Alternate gene names: 114798205

Gene position: 454360-455154 (Clockwise)

Preceding gene: 114799399

Following gene: 114798833

Centisome position: 12.26

GC content: 61.38

Gene sequence:

>795_bases
ATGCAGCAATATCAGGCACTTCTGAGAGACATCCTGGAGAACGGCCACAAGCGGGGCGACCGCACGGGCGTCGGCACGAT
CGGCGTCTTCGGCCGCCAGATGCGGTTTGACCTCTCCGAAGGCTTCCCGATGGTGACCACCAAACGCCTCCACCTGCGCT
CGATCATCGTCGAGCTGCTCTGGTTCCTGCGCGGTGACACCAACATCGCGTATCTGAAGGAAAATGGCGTCTCCATCTGG
GATGAATGGGCCGATGAGAACGGCGACCTAGGCCCCGTCTATGGCAAGCAATGGCGCAGCTGGGCCACGCCGAATGGCGA
AGCCATCGACCAGATCCAATGGGTGCTGAACGAAATCCGCACCAATCCCAATTCCCGCCGCCTGATCGTTTCGGCCTGGA
ACCCGGCGGACGTAAACGACATGGCCCTGCCGCCCTGCCACTGCCTGTTCCAGTTCAATGTCATGGACGGCAAGCTGAAC
TGCCAGCTTTACCAGCGCTCGGCAGACGTCTTCCTCGGCGTGCCCTTCAACATCGCCTCCTACGCGCTCCTCACAATGAT
GATGGCCCGCGCTACGGGGCTTCAGCCCGGCGAATTCGTCCACACCTTCGGCGACGCCCACCTCTATCTGAACCATCTCG
AACAGGCAGAGCTCCAGCTCACCCGCGAGCCGCGCGATCTGCCCACCATCCATATGAACCCGGACAAGGCAGACCTCTTC
GGATGGGAGTATGAGGATTTCCGCGTCGACGGATATGCCCCCCACCCTCACATCAAGGCGCCCGTCGCAGTCTGA

Upstream 100 bases:

>100_bases
GATGTGGGTTTCTGCCCCGTAAGGCGCGTGCGATCAAATCTGCCCAGCCAGTTTGGAATCTGTTAACCTGAGCTCCGCGC
GAATCGAGAGGCCCTGGCAA

Downstream 100 bases:

>100_bases
TGGGGCTGTCTGACGCGGCCTACTCGCTCAGCAGCTCGCAATGAATGAACCCGCCGGTTTCCACGGCCAGCGTCAGGGCC
GCCTCGAACTCGGTGCAGTA

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MQQYQALLRDILENGHKRGDRTGVGTIGVFGRQMRFDLSEGFPMVTTKRLHLRSIIVELLWFLRGDTNIAYLKENGVSIW
DEWADENGDLGPVYGKQWRSWATPNGEAIDQIQWVLNEIRTNPNSRRLIVSAWNPADVNDMALPPCHCLFQFNVMDGKLN
CQLYQRSADVFLGVPFNIASYALLTMMMARATGLQPGEFVHTFGDAHLYLNHLEQAELQLTREPRDLPTIHMNPDKADLF
GWEYEDFRVDGYAPHPHIKAPVAV

Sequences:

>Translated_264_residues
MQQYQALLRDILENGHKRGDRTGVGTIGVFGRQMRFDLSEGFPMVTTKRLHLRSIIVELLWFLRGDTNIAYLKENGVSIW
DEWADENGDLGPVYGKQWRSWATPNGEAIDQIQWVLNEIRTNPNSRRLIVSAWNPADVNDMALPPCHCLFQFNVMDGKLN
CQLYQRSADVFLGVPFNIASYALLTMMMARATGLQPGEFVHTFGDAHLYLNHLEQAELQLTREPRDLPTIHMNPDKADLF
GWEYEDFRVDGYAPHPHIKAPVAV
>Mature_264_residues
MQQYQALLRDILENGHKRGDRTGVGTIGVFGRQMRFDLSEGFPMVTTKRLHLRSIIVELLWFLRGDTNIAYLKENGVSIW
DEWADENGDLGPVYGKQWRSWATPNGEAIDQIQWVLNEIRTNPNSRRLIVSAWNPADVNDMALPPCHCLFQFNVMDGKLN
CQLYQRSADVFLGVPFNIASYALLTMMMARATGLQPGEFVHTFGDAHLYLNHLEQAELQLTREPRDLPTIHMNPDKADLF
GWEYEDFRVDGYAPHPHIKAPVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=282, Percent_Identity=49.290780141844, Blast_Score=285, Evalue=4e-77,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=63.6363636363636, Blast_Score=367, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI71993377, Length=283, Percent_Identity=44.5229681978799, Blast_Score=254, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6324648, Length=230, Percent_Identity=45.2173913043478, Blast_Score=201, Evalue=8e-53,
Organism=Drosophila melanogaster, GI17137556, Length=284, Percent_Identity=51.056338028169, Blast_Score=286, Evalue=8e-78,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_HYPNA (Q0C504)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_759189.1
- ProteinModelPortal:   Q0C504
- SMR:   Q0C504
- STRING:   Q0C504
- GeneID:   4287829
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_0459
- NMPDR:   fig|228405.5.peg.452
- TIGR:   HNE_0459
- eggNOG:   COG0207
- HOGENOM:   HBG588098
- OMA:   RMALAPC
- PhylomeDB:   Q0C504
- ProtClustDB:   PRK01827
- BioCyc:   HNEP81032:HNE_0459-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 30197; Mature: 30197

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 146-146

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQYQALLRDILENGHKRGDRTGVGTIGVFGRQMRFDLSEGFPMVTTKRLHLRSIIVELL
CHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCEEEECCCCCCEEEHHHHHHHHHHHHHH
WFLRGDTNIAYLKENGVSIWDEWADENGDLGPVYGKQWRSWATPNGEAIDQIQWVLNEIR
HHHCCCCCEEEEECCCCEEHHHHCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHH
TNPNSRRLIVSAWNPADVNDMALPPCHCLFQFNVMDGKLNCQLYQRSADVFLGVPFNIAS
CCCCCCEEEEEECCCCCCCCCCCCCEEEEEEEEEECCEEEEEEEECCCCEEEECCHHHHH
YALLTMMMARATGLQPGEFVHTFGDAHLYLNHLEQAELQLTREPRDLPTIHMNPDKADLF
HHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHC
GWEYEDFRVDGYAPHPHIKAPVAV
CCCCCCEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MQQYQALLRDILENGHKRGDRTGVGTIGVFGRQMRFDLSEGFPMVTTKRLHLRSIIVELL
CHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCEEEECCCCCCEEEHHHHHHHHHHHHHH
WFLRGDTNIAYLKENGVSIWDEWADENGDLGPVYGKQWRSWATPNGEAIDQIQWVLNEIR
HHHCCCCCEEEEECCCCEEHHHHCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHH
TNPNSRRLIVSAWNPADVNDMALPPCHCLFQFNVMDGKLNCQLYQRSADVFLGVPFNIAS
CCCCCCEEEEEECCCCCCCCCCCCCEEEEEEEEEECCEEEEEEEECCCCEEEECCHHHHH
YALLTMMMARATGLQPGEFVHTFGDAHLYLNHLEQAELQLTREPRDLPTIHMNPDKADLF
HHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHC
GWEYEDFRVDGYAPHPHIKAPVAV
CCCCCCEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA