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The map label for this gene is cysS

Identifier: 114797963

GI number: 114797963

Start: 3374796

End: 3376181

Strand: Reverse

Name: cysS

Synonym: HNE_3201

Alternate gene names: 114797963

Gene position: 3376181-3374796 (Counterclockwise)

Preceding gene: 114798758

Following gene: 114797483

Centisome position: 91.12

GC content: 62.05

Gene sequence:

>1386_bases
ATGACCCTCCGCATTTATGACACTGCCGCGCGCAAGAAGCGCGTCTTCGAGCCCCAGGACGCGAAGCGGGTTACCCTGTA
TGTCTGCGGCCCGACGGTGTATAATTACGCCCACATCGGGAATGCGCGTCCACCGGTGGTGTTTGATGTGCTGCGGCGCG
TTCTGATGGCCCGTTATGGTGAAGATGCGATCGTCTATGCGCGCAATATCACGGACATCGAAGACAAGATCATCGCAGCC
AGCGTGGCCAGCGGCGAGCCGATTTCGGCCATCACACAGAAATATGCGGCCATTTATAACGCCGATGTAGAGGCGCTGAA
CGTCATCGCCCCTACCATCGAGCCTTGGGCGACGGGCCATGTGCCCGAAATGATCGAGATCATCGAAAAGCTCATCCGCA
AGAAATACGCCTATGTCGGCGACACCGGCGTCTGGTTCTCTGTGCCCTCGATGCCCGATTACGGGCGTCTCTCAGGACGC
AAGCCCGAGGATAATGAAGCCGGCGCGCGCGTGGCCGTGGATGAAGACAAGCGCGATCCTTCGGACTTTGCGCTGTGGAA
GTTTGCCAAGCCCGGCGAGCCCGAAGACGCGATCTGGGACAGCCCCTGGGGCCGCGGCCGCCCTGGCTGGCACATCGAAT
GCTCGGCGATGGCAGCCAAACACCTTGGCCGCACGATCGACATTCATGGCGGCGGCGTGGACCTGACCTTCCCGCATCAT
GAAAACGAGATCGCTCAGTCAGAATGCGCCCATGGTGAAGAACTCGCCCGCTACTGGATGCATAACGGCTTCCTCGACAT
GGGCGGGGAGAAGATGTCGAAATCGCTGGGGAATGTCGTGACCGTTCACGACCTGCTGAAGGCCTGGCCGGGCGAAGTGC
TGCGTTTTGCGCTGCTGACCGCCCATTACCGCGCCCAGCTCGACTGGACCGAAGACCTGCTGAAACAGGCCAAGACCACG
CTCGACCGGATTTATGGCGCGCTGCGCCGGGTCTGGGAAGCAGACGGCGGCGAAGCGCGCGACACCGGCGTTCTGCGCGC
GCTGGAGGATGATCTCGGCACGCCGGACGCGCTCGCTGAGCTCGCACGGCTCGCCAGTGAAGCCAATACAGCGGCTGATC
TGAAAGATGCCGTTGCCATGGCGAATGGCCGGGCAAACCTTCTGGCGGCAGGGAAACTGCTTGGCCTGCTGACCAAAACA
CCCAAAGAATGGGAGCAGGGCGCCGATACGGACGAAAATTCCCGCATTGACGCGCAGGTGCAGGCCCGCGTCGATGCACG
CGTCGCCAAGGACTGGGCCGAAGCGGACCGTATCCGCAAGGCGCTGGCAGAGGAGGGGGTCGAAATCATGGACGGTCCAG
CAGGCAGCACATGGCGGCGCATCTGA

Upstream 100 bases:

>100_bases
GCCGTCCCTGTTGCTGCTGAGGCTGCAACGGGTCATAGGGCTGCCCGGACGCCAATAGCTCGCGCGCTGATTTCACGACA
TTCCCGATCCAAGAGATACG

Downstream 100 bases:

>100_bases
TCCGGCGCAGTTTTGTCTTTTTTCTTGCCGCGAGCGTTCTTGCGGCCTGCAGTACACCTGTTGCCCCTGTATCCGGCGCC
GAGGATGTGACCCGGATTTT

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MTLRIYDTAARKKRVFEPQDAKRVTLYVCGPTVYNYAHIGNARPPVVFDVLRRVLMARYGEDAIVYARNITDIEDKIIAA
SVASGEPISAITQKYAAIYNADVEALNVIAPTIEPWATGHVPEMIEIIEKLIRKKYAYVGDTGVWFSVPSMPDYGRLSGR
KPEDNEAGARVAVDEDKRDPSDFALWKFAKPGEPEDAIWDSPWGRGRPGWHIECSAMAAKHLGRTIDIHGGGVDLTFPHH
ENEIAQSECAHGEELARYWMHNGFLDMGGEKMSKSLGNVVTVHDLLKAWPGEVLRFALLTAHYRAQLDWTEDLLKQAKTT
LDRIYGALRRVWEADGGEARDTGVLRALEDDLGTPDALAELARLASEANTAADLKDAVAMANGRANLLAAGKLLGLLTKT
PKEWEQGADTDENSRIDAQVQARVDARVAKDWAEADRIRKALAEEGVEIMDGPAGSTWRRI

Sequences:

>Translated_461_residues
MTLRIYDTAARKKRVFEPQDAKRVTLYVCGPTVYNYAHIGNARPPVVFDVLRRVLMARYGEDAIVYARNITDIEDKIIAA
SVASGEPISAITQKYAAIYNADVEALNVIAPTIEPWATGHVPEMIEIIEKLIRKKYAYVGDTGVWFSVPSMPDYGRLSGR
KPEDNEAGARVAVDEDKRDPSDFALWKFAKPGEPEDAIWDSPWGRGRPGWHIECSAMAAKHLGRTIDIHGGGVDLTFPHH
ENEIAQSECAHGEELARYWMHNGFLDMGGEKMSKSLGNVVTVHDLLKAWPGEVLRFALLTAHYRAQLDWTEDLLKQAKTT
LDRIYGALRRVWEADGGEARDTGVLRALEDDLGTPDALAELARLASEANTAADLKDAVAMANGRANLLAAGKLLGLLTKT
PKEWEQGADTDENSRIDAQVQARVDARVAKDWAEADRIRKALAEEGVEIMDGPAGSTWRRI
>Mature_460_residues
TLRIYDTAARKKRVFEPQDAKRVTLYVCGPTVYNYAHIGNARPPVVFDVLRRVLMARYGEDAIVYARNITDIEDKIIAAS
VASGEPISAITQKYAAIYNADVEALNVIAPTIEPWATGHVPEMIEIIEKLIRKKYAYVGDTGVWFSVPSMPDYGRLSGRK
PEDNEAGARVAVDEDKRDPSDFALWKFAKPGEPEDAIWDSPWGRGRPGWHIECSAMAAKHLGRTIDIHGGGVDLTFPHHE
NEIAQSECAHGEELARYWMHNGFLDMGGEKMSKSLGNVVTVHDLLKAWPGEVLRFALLTAHYRAQLDWTEDLLKQAKTTL
DRIYGALRRVWEADGGEARDTGVLRALEDDLGTPDALAELARLASEANTAADLKDAVAMANGRANLLAAGKLLGLLTKTP
KEWEQGADTDENSRIDAQVQARVDARVAKDWAEADRIRKALAEEGVEIMDGPAGSTWRRI

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=390, Percent_Identity=38.2051282051282, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI21269877, Length=345, Percent_Identity=33.0434782608696, Blast_Score=201, Evalue=9e-52,
Organism=Homo sapiens, GI62240992, Length=345, Percent_Identity=33.0434782608696, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI10835051, Length=345, Percent_Identity=33.0434782608696, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI303304987, Length=345, Percent_Identity=33.0434782608696, Blast_Score=201, Evalue=1e-51,
Organism=Escherichia coli, GI1786737, Length=467, Percent_Identity=48.8222698072805, Blast_Score=412, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17509795, Length=436, Percent_Identity=29.8165137614679, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17509797, Length=436, Percent_Identity=29.8165137614679, Blast_Score=159, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6324082, Length=332, Percent_Identity=34.9397590361446, Blast_Score=206, Evalue=6e-54,
Organism=Drosophila melanogaster, GI19922218, Length=512, Percent_Identity=34.5703125, Blast_Score=251, Evalue=8e-67,
Organism=Drosophila melanogaster, GI19922364, Length=262, Percent_Identity=38.9312977099237, Blast_Score=192, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_HYPNA (Q0BXB7)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_761876.1
- ProteinModelPortal:   Q0BXB7
- STRING:   Q0BXB7
- GeneID:   4289575
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_3201
- NMPDR:   fig|228405.5.peg.3066
- TIGR:   HNE_3201
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- PhylomeDB:   Q0BXB7
- ProtClustDB:   PRK00260
- BioCyc:   HNEP81032:HNE_3201-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 51009; Mature: 50878

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 274-274

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLRIYDTAARKKRVFEPQDAKRVTLYVCGPTVYNYAHIGNARPPVVFDVLRRVLMARYG
CEEEEECCHHHHHCCCCCCCCCEEEEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHHHCC
EDAIVYARNITDIEDKIIAASVASGEPISAITQKYAAIYNADVEALNVIAPTIEPWATGH
CCEEEEECCCCCHHHHHHEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCC
VPEMIEIIEKLIRKKYAYVGDTGVWFSVPSMPDYGRLSGRKPEDNEAGARVAVDEDKRDP
HHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCC
SDFALWKFAKPGEPEDAIWDSPWGRGRPGWHIECSAMAAKHLGRTIDIHGGGVDLTFPHH
CCCEEEECCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCEEEEECCCEEEECCCC
ENEIAQSECAHGEELARYWMHNGFLDMGGEKMSKSLGNVVTVHDLLKAWPGEVLRFALLT
CCHHHHHHHCCHHHHHHHHHHCCCHHCCHHHHHHHHCCEEHHHHHHHHCHHHHHHHHHHH
AHYRAQLDWTEDLLKQAKTTLDRIYGALRRVWEADGGEARDTGVLRALEDDLGTPDALAE
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHH
LARLASEANTAADLKDAVAMANGRANLLAAGKLLGLLTKTPKEWEQGADTDENSRIDAQV
HHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCHHH
QARVDARVAKDWAEADRIRKALAEEGVEIMDGPAGSTWRRI
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCC
>Mature Secondary Structure 
TLRIYDTAARKKRVFEPQDAKRVTLYVCGPTVYNYAHIGNARPPVVFDVLRRVLMARYG
EEEEECCHHHHHCCCCCCCCCEEEEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHHHCC
EDAIVYARNITDIEDKIIAASVASGEPISAITQKYAAIYNADVEALNVIAPTIEPWATGH
CCEEEEECCCCCHHHHHHEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCC
VPEMIEIIEKLIRKKYAYVGDTGVWFSVPSMPDYGRLSGRKPEDNEAGARVAVDEDKRDP
HHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCC
SDFALWKFAKPGEPEDAIWDSPWGRGRPGWHIECSAMAAKHLGRTIDIHGGGVDLTFPHH
CCCEEEECCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCEEEEECCCEEEECCCC
ENEIAQSECAHGEELARYWMHNGFLDMGGEKMSKSLGNVVTVHDLLKAWPGEVLRFALLT
CCHHHHHHHCCHHHHHHHHHHCCCHHCCHHHHHHHHCCEEHHHHHHHHCHHHHHHHHHHH
AHYRAQLDWTEDLLKQAKTTLDRIYGALRRVWEADGGEARDTGVLRALEDDLGTPDALAE
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHH
LARLASEANTAADLKDAVAMANGRANLLAAGKLLGLLTKTPKEWEQGADTDENSRIDAQV
HHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCHHH
QARVDARVAKDWAEADRIRKALAEEGVEIMDGPAGSTWRRI
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA