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The map label for this gene is ligA

Identifier: 114797923

GI number: 114797923

Start: 378661

End: 380766

Strand: Direct

Name: ligA

Synonym: HNE_0386

Alternate gene names: 114797923

Gene position: 378661-380766 (Clockwise)

Preceding gene: 114797433

Following gene: 114799059

Centisome position: 10.22

GC content: 64.96

Gene sequence:

>2106_bases
ATGAGCGGGGACAAACCAGTCGAGGCGCTGAGCGAAGCGGAAGCGGCGGCAGAACTGGAGCGGCTCGCGCGGGCAATTGC
GGACGCTGACGCGGCCTATTACCAGAATGATGCGCCCGAGCTGACCGACGCTGACTATGACGCGCTGCGCCAGCGCAACC
TCGCCATCGAGGCGCGCTTTCCGGCGCTGAAGCGCGAGGATTCGCCGACCGACCGGGTGGGCGCCGAAGCCGGAGACGGC
TTTGCCAAGGCGCGTCACAGCGCGCCGATGCTTTCGCTCGACAATGCGTTCACGGATGAAGACGTCGCAGACTTCGCGGT
TCGCATCCGGCGGTTTCTGGGGCTGGCGCCGGAGGAAACGCTCGCCTTTACCGCAGAACCCAAGATCGACGGGCTGTCGC
TGAGCCTGACCTATGAAAACGGAAAACTGATGCGCGCGGCAACGCGCGGCGATGGCCAGACCGGTGAGGACGTGACCGCG
AACGCCCGCACGCTGGGCGACATTCCGGTGAAGCTGAAAGGCAAGGGCTGGCCGGAGCGGATCGAGGTGCGCGGCGAGGT
CTATATGGCGAAATCTGCCTTTGCCGATCTCAACGCGCGCGAGGCAGAGGCCGGGCGGAAGGTGTTTGCCAATCCGCGCA
ATGCGGCCGCCGGGAGCCTGCGCCAGCTGGATGTCGAGATTACCAAATCGCGGCCTTTGCGCTTTTTCGCCTATGCCTGG
GCGGCGGCGAGCGAGCCTTTTGCGCAGACCCAGTTTGAGGCTGTTAAGGCGTTTGCCGATTGGGGTTTTGTCACCAATTC
CCGCATGATCCGTATCAAGACGGTAGAGGAAGTTCTCTCCTATTATCAGGAGATCGAGGCGGAACGGGCGAGCCTCGAAT
ACGATATTGACGGGGTGGTCTATAAGGTCGACCGGCTGGACTGGCAGCAGCGGCTGGGCTTTGTCAGCCGCGCGCCGCGC
TGGGCTATTGCGCACAAGTTTCCGGCCGAGAAGGCGGTCACAACGCTGCTGGGCATCGACATTCAGGTGGGGCGGACAGG
GTCGCTGACGCCGGTGGCGCGGCTGGAGCCGGTGACGGTGGGCGGTGTCGTGGTCTCAAACGCCACACTGCATAATGAAG
ACGAAATCGCGCGCCTCGGCGTCAAGCCCGGAGACAAGGTTGAGATTCAGCGGGCGGGCGATGTCATCCCGCAGGTGCTC
CGCGTGGTCGAGCCGGGGCAGGGTGCGCCCTGGGCGATGCCGGACACATGCCCGGTCTGTGGCTCGGCAGCTGTGCGCGA
GATTGATGATGCCGGGCGCGCCGATGTGCGCCGGCGCTGCACGGGCGGGCTGATCTGCCCGGCCCAGGCGGTCGAGCGGC
TCAAGCATTTCGTCTCGCGCAAGGCGCTCGATATTGACGGGCTTGGCGAGAAGCAAGTGCTGCTGTTCCATGAGAAAGGC
GCGGTGAAAGCGCCCCAGGATATATTCCGCCTGAAGAAGAATATCGAGGCGGCCGGCCTGCCGCCGCTGGAGGAATGGGA
GGGCTTTGGCGCGCAGTCTGCCAGGAAGCTTTACAGCGCGATTGATGCGCGCCGCAAAGTGCCCTTCGCGCGGTTTCTGA
ACGGCCTTGGCATCCGCTATGTCGGCCAGACGACCTCGGCCCAGTTTGCCCGCAGTTTCCTCAGCTGGCAGAGCTTCTGG
GCGGCGGTGAAAGCGGCCGAAGAGGGCGGCATCGAGAGCGAGGCGTATAATGAGCTCATCGGCATTGACGGCATCGGCCA
GGCCGCGGCCCGCTCGCTGATGGCGTTTGAGGGCGAGCCGCACAATCGCGAGATGCTGGCCGCGCTGCTGGAAGAGGTTG
AGGTGGAGGACGAGATCCCCGCCGAGACCGGCAGCCCGGTGACGGGTAAGACGGTGGTGTTCACCGGCACGCTGGAGAAG
ATGACGCGCGACGAGGCCAAGGCACGGGCGAGCGCGCTGGGCGCGAAGGTTGCCGGCACGGTTTCGGCCAAGACAGATAT
TGTGATTGCCGGGCCGGGCGCAGGCTCAAAGCTCGTCAAGGCCGAGCAGCTCGGCCTCGCGGTGATGACGGAAGATGAAT
GGCTCGCCCTGATCGGGGAGACCTGA

Upstream 100 bases:

>100_bases
TAGATGCGTGCGAGCGGCGGGAAGAGATTGCCCGCATGCTGTCCGGCGCCGAAGTCACCGATGAGGCGCGCGCTGCGGCA
GGGAGGTTGATGGAGGATGC

Downstream 100 bases:

>100_bases
GGGGCAGGGGCCCGGCGGGTTATTTGCCGCCGGGGATCAGCCGCTGGAGGAAGGTCCGGCGCGTGGTTTCGTCAACCGCT
TCGGGGTTCAGCCCTTCATT

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 701; Mature: 700

Protein sequence:

>701_residues
MSGDKPVEALSEAEAAAELERLARAIADADAAYYQNDAPELTDADYDALRQRNLAIEARFPALKREDSPTDRVGAEAGDG
FAKARHSAPMLSLDNAFTDEDVADFAVRIRRFLGLAPEETLAFTAEPKIDGLSLSLTYENGKLMRAATRGDGQTGEDVTA
NARTLGDIPVKLKGKGWPERIEVRGEVYMAKSAFADLNAREAEAGRKVFANPRNAAAGSLRQLDVEITKSRPLRFFAYAW
AAASEPFAQTQFEAVKAFADWGFVTNSRMIRIKTVEEVLSYYQEIEAERASLEYDIDGVVYKVDRLDWQQRLGFVSRAPR
WAIAHKFPAEKAVTTLLGIDIQVGRTGSLTPVARLEPVTVGGVVVSNATLHNEDEIARLGVKPGDKVEIQRAGDVIPQVL
RVVEPGQGAPWAMPDTCPVCGSAAVREIDDAGRADVRRRCTGGLICPAQAVERLKHFVSRKALDIDGLGEKQVLLFHEKG
AVKAPQDIFRLKKNIEAAGLPPLEEWEGFGAQSARKLYSAIDARRKVPFARFLNGLGIRYVGQTTSAQFARSFLSWQSFW
AAVKAAEEGGIESEAYNELIGIDGIGQAAARSLMAFEGEPHNREMLAALLEEVEVEDEIPAETGSPVTGKTVVFTGTLEK
MTRDEAKARASALGAKVAGTVSAKTDIVIAGPGAGSKLVKAEQLGLAVMTEDEWLALIGET

Sequences:

>Translated_701_residues
MSGDKPVEALSEAEAAAELERLARAIADADAAYYQNDAPELTDADYDALRQRNLAIEARFPALKREDSPTDRVGAEAGDG
FAKARHSAPMLSLDNAFTDEDVADFAVRIRRFLGLAPEETLAFTAEPKIDGLSLSLTYENGKLMRAATRGDGQTGEDVTA
NARTLGDIPVKLKGKGWPERIEVRGEVYMAKSAFADLNAREAEAGRKVFANPRNAAAGSLRQLDVEITKSRPLRFFAYAW
AAASEPFAQTQFEAVKAFADWGFVTNSRMIRIKTVEEVLSYYQEIEAERASLEYDIDGVVYKVDRLDWQQRLGFVSRAPR
WAIAHKFPAEKAVTTLLGIDIQVGRTGSLTPVARLEPVTVGGVVVSNATLHNEDEIARLGVKPGDKVEIQRAGDVIPQVL
RVVEPGQGAPWAMPDTCPVCGSAAVREIDDAGRADVRRRCTGGLICPAQAVERLKHFVSRKALDIDGLGEKQVLLFHEKG
AVKAPQDIFRLKKNIEAAGLPPLEEWEGFGAQSARKLYSAIDARRKVPFARFLNGLGIRYVGQTTSAQFARSFLSWQSFW
AAVKAAEEGGIESEAYNELIGIDGIGQAAARSLMAFEGEPHNREMLAALLEEVEVEDEIPAETGSPVTGKTVVFTGTLEK
MTRDEAKARASALGAKVAGTVSAKTDIVIAGPGAGSKLVKAEQLGLAVMTEDEWLALIGET
>Mature_700_residues
SGDKPVEALSEAEAAAELERLARAIADADAAYYQNDAPELTDADYDALRQRNLAIEARFPALKREDSPTDRVGAEAGDGF
AKARHSAPMLSLDNAFTDEDVADFAVRIRRFLGLAPEETLAFTAEPKIDGLSLSLTYENGKLMRAATRGDGQTGEDVTAN
ARTLGDIPVKLKGKGWPERIEVRGEVYMAKSAFADLNAREAEAGRKVFANPRNAAAGSLRQLDVEITKSRPLRFFAYAWA
AASEPFAQTQFEAVKAFADWGFVTNSRMIRIKTVEEVLSYYQEIEAERASLEYDIDGVVYKVDRLDWQQRLGFVSRAPRW
AIAHKFPAEKAVTTLLGIDIQVGRTGSLTPVARLEPVTVGGVVVSNATLHNEDEIARLGVKPGDKVEIQRAGDVIPQVLR
VVEPGQGAPWAMPDTCPVCGSAAVREIDDAGRADVRRRCTGGLICPAQAVERLKHFVSRKALDIDGLGEKQVLLFHEKGA
VKAPQDIFRLKKNIEAAGLPPLEEWEGFGAQSARKLYSAIDARRKVPFARFLNGLGIRYVGQTTSAQFARSFLSWQSFWA
AVKAAEEGGIESEAYNELIGIDGIGQAAARSLMAFEGEPHNREMLAALLEEVEVEDEIPAETGSPVTGKTVVFTGTLEKM
TRDEAKARASALGAKVAGTVSAKTDIVIAGPGAGSKLVKAEQLGLAVMTEDEWLALIGET

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=693, Percent_Identity=46.3203463203463, Blast_Score=567, Evalue=1e-163,
Organism=Escherichia coli, GI87082305, Length=572, Percent_Identity=24.3006993006993, Blast_Score=112, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_HYPNA (Q0C577)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_759116.1
- ProteinModelPortal:   Q0C577
- STRING:   Q0C577
- GeneID:   4287273
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_0386
- NMPDR:   fig|228405.5.peg.380
- TIGR:   HNE_0386
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   Q0C577
- BioCyc:   HNEP81032:HNE_0386-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 75960; Mature: 75828

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 128-128 BINDING 126-126 BINDING 149-149 BINDING 186-186 BINDING 302-302 BINDING 326-326

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGDKPVEALSEAEAAAELERLARAIADADAAYYQNDAPELTDADYDALRQRNLAIEARF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCHHHHHHCCCEEEECC
PALKREDSPTDRVGAEAGDGFAKARHSAPMLSLDNAFTDEDVADFAVRIRRFLGLAPEET
CCCCCCCCCCHHCCCCCCCCHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCHH
LAFTAEPKIDGLSLSLTYENGKLMRAATRGDGQTGEDVTANARTLGDIPVKLKGKGWPER
EEEECCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCCCCCCEECCCCCEEECCCCCCHH
IEVRGEVYMAKSAFADLNAREAEAGRKVFANPRNAAAGSLRQLDVEITKSRPLRFFAYAW
EEECCEEEEEHHHHHCCCCHHHHCCCHHHCCCCCCCCCCCEEEEEEEECCCCEEEEEEHH
AAASEPFAQTQFEAVKAFADWGFVTNSRMIRIKTVEEVLSYYQEIEAERASLEYDIDGVV
HHCCCCHHHHHHHHHHHHHHCCEEECCCEEEEEHHHHHHHHHHHHHHHHHCCEECCCCEE
YKVDRLDWQQRLGFVSRAPRWAIAHKFPAEKAVTTLLGIDIQVGRTGSLTPVARLEPVTV
EEEHHCCHHHHHHHHHCCCCEEHHCCCCHHHHHHHHHCCEEEECCCCCCCCCCCCCCEEE
GGVVVSNATLHNEDEIARLGVKPGDKVEIQRAGDVIPQVLRVVEPGQGAPWAMPDTCPVC
CCEEEECCEECCCCHHHCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
GSAAVREIDDAGRADVRRRCTGGLICPAQAVERLKHFVSRKALDIDGLGEKQVLLFHEKG
CCHHHHHHHCCCHHHHHHHHCCCEECHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCC
AVKAPQDIFRLKKNIEAAGLPPLEEWEGFGAQSARKLYSAIDARRKVPFARFLNGLGIRY
CCCCCHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCEEE
VGQTTSAQFARSFLSWQSFWAAVKAAEEGGIESEAYNELIGIDGIGQAAARSLMAFEGEP
ECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCC
HNREMLAALLEEVEVEDEIPAETGSPVTGKTVVFTGTLEKMTRDEAKARASALGAKVAGT
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCHHHEEE
VSAKTDIVIAGPGAGSKLVKAEQLGLAVMTEDEWLALIGET
EECCCCEEEECCCCCCHHHHHHHCCEEEEECCCEEEEECCC
>Mature Secondary Structure 
SGDKPVEALSEAEAAAELERLARAIADADAAYYQNDAPELTDADYDALRQRNLAIEARF
CCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCHHHHHHCCCEEEECC
PALKREDSPTDRVGAEAGDGFAKARHSAPMLSLDNAFTDEDVADFAVRIRRFLGLAPEET
CCCCCCCCCCHHCCCCCCCCHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCHH
LAFTAEPKIDGLSLSLTYENGKLMRAATRGDGQTGEDVTANARTLGDIPVKLKGKGWPER
EEEECCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCCCCCCEECCCCCEEECCCCCCHH
IEVRGEVYMAKSAFADLNAREAEAGRKVFANPRNAAAGSLRQLDVEITKSRPLRFFAYAW
EEECCEEEEEHHHHHCCCCHHHHCCCHHHCCCCCCCCCCCEEEEEEEECCCCEEEEEEHH
AAASEPFAQTQFEAVKAFADWGFVTNSRMIRIKTVEEVLSYYQEIEAERASLEYDIDGVV
HHCCCCHHHHHHHHHHHHHHCCEEECCCEEEEEHHHHHHHHHHHHHHHHHCCEECCCCEE
YKVDRLDWQQRLGFVSRAPRWAIAHKFPAEKAVTTLLGIDIQVGRTGSLTPVARLEPVTV
EEEHHCCHHHHHHHHHCCCCEEHHCCCCHHHHHHHHHCCEEEECCCCCCCCCCCCCCEEE
GGVVVSNATLHNEDEIARLGVKPGDKVEIQRAGDVIPQVLRVVEPGQGAPWAMPDTCPVC
CCEEEECCEECCCCHHHCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
GSAAVREIDDAGRADVRRRCTGGLICPAQAVERLKHFVSRKALDIDGLGEKQVLLFHEKG
CCHHHHHHHCCCHHHHHHHHCCCEECHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCC
AVKAPQDIFRLKKNIEAAGLPPLEEWEGFGAQSARKLYSAIDARRKVPFARFLNGLGIRY
CCCCCHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCEEE
VGQTTSAQFARSFLSWQSFWAAVKAAEEGGIESEAYNELIGIDGIGQAAARSLMAFEGEP
ECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCC
HNREMLAALLEEVEVEDEIPAETGSPVTGKTVVFTGTLEKMTRDEAKARASALGAKVAGT
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCHHHEEE
VSAKTDIVIAGPGAGSKLVKAEQLGLAVMTEDEWLALIGET
EECCCCEEEECCCCCCHHHHHHHCCEEEEECCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA