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The map label for this gene is kefA [H]

Identifier: 114797807

GI number: 114797807

Start: 529082

End: 530533

Strand: Direct

Name: kefA [H]

Synonym: HNE_0534

Alternate gene names: 114797807

Gene position: 529082-530533 (Clockwise)

Preceding gene: 114797313

Following gene: 114798752

Centisome position: 14.28

GC content: 62.26

Gene sequence:

>1452_bases
ATGCCTTTGAGGATTGCCGCAAGCATGAGCCTCGACCTGCTCCAGCCGGAAACTGCCGCTCCGCTGATCGCCGAACTTCA
GGAGTCGGTCACCCGGCTGATCGAGGCGGCACGCGATGAGTTAAGCTCGCCCGACACACTCTGGCAGGTCGGCGCGCTGA
TCCTCGGCGCGGCGCTCGGCTACATCATCTCCCGCTTCCCCAAGACGATGCTGATGCAGGCGGCGGAAAAGCGCGGGCGG
ATCGATTTCGTCCTGCTCACCTACCATTCCCTCTCGCGCATCGTCTGGCCGGTCTTCACGCTCATCCTGCTGGCGGCCGC
CACCGCCGCCTTCGAGGCTGTCGGCCTGGGCAACGCCACGCTGCACATCGCCACCAGCCTCCTCCTGGCCTGGATCGTCG
TGCGCATCTTCACCGCCAGCATGCGGCGCGGCTTCTGGTCGAAAGCCATCGCCTATTTCGCCTGGGCGATCACCGCGCTC
TACATCCTCGGCTGGCTGCGCCCGCTGACGGGCTGGCTGAACAGTGTCACCTTCGATTTCGGCGAGAGCGAAGTCTCCGT
CCTGCGCATCCTGATCAGCCTCGCGCTGGCGGCGTTCGCCATCTGGCTCGGCCGGCTCATCGGCAATGTCGCCCAGTCCC
AGCTGCGCGGTTCGCCGGAACTGCCTGCTTCGGTCGGCGGTCTTCTGGGCCAGAGCATCAAGATCGGCGTGATGATCGTC
GCCGTCCTGATCGCGCTCAACTTCCTCGGCGTGAATGTCGCCGCGCTCACCTTCTTCTCAGGTGCGCTTGGTGTCGGCAT
CGGCTTTGGCTTGCAGTCGGTCTTCTCCAACTTCATCTCCGGCGTCATCATCCTGATGGAGAAGTCGCTGAAAGTCGGCG
ATTTCATCGAGCTTCAGTCCGGCCTCTCCGGCGTGGTGAAGGAGATCAACTTCCGCTCCACCGTCGTGATGACGAACGAT
AATGTCGACATCATCGTTCCCAACGAAGCCTTCATCAAAGCCCAGCTGATCAACTGGACGATGACCGAGGCGCGCCGGCG
CATCCACGTCCCGTTTGGGGTGGCCTATGGCACGGATAAAGCGCTGGTCGAAAAGGCCGGCCTGGAAGCCGCCGCCAGCG
TGCCCTGGACCTTCGACGATCGGGGCGAGCACACCCCGCAGGTCTGGCTCGTCAAGTTCGGCGACTTCCGCCTCGAATTC
GAGCTGATTGTCTGGCTGACCGACGAAGCCGTGCTCCGTCCGCAGAAGGTCATGGCAGATTATGTGTGGGCGGTTCACTC
GGCAATCGAGAAATACGAGATCCATATCCCGATGCCCCAGCGCGACCTGCATTTCAAATCGCCTGGAACACTCAGTGTTC
GGATCGAAACGGGAGAGGCAGAGAAGCCCGCGCCGGACGCAGAAGACGCGCCTGAAAAAGCATCAGCAGAAGCATCAGAA
ACAAAAAGCTGA

Upstream 100 bases:

>100_bases
CCTGAATGAGCGCTGCGAACTGGTCCTCAAGCGCATCTGACGCGCACAGGCAGGGTTGCATGCATAGGCGAGCCCGATAA
GGTTTCCCCTTGTCGATTCA

Downstream 100 bases:

>100_bases
TGCCGCTGCCGTCCGGGGGAAATGACGGCAGCGGCGGGACGGCCCGAGGGGTGCGGGCCTAGTCCAGGGCTTTTACAAAT
TTCCGCGCAAAGCGGTCCGA

Product: small conductance mechanosensitive ion channel (MscS) family protein

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MPLRIAASMSLDLLQPETAAPLIAELQESVTRLIEAARDELSSPDTLWQVGALILGAALGYIISRFPKTMLMQAAEKRGR
IDFVLLTYHSLSRIVWPVFTLILLAAATAAFEAVGLGNATLHIATSLLLAWIVVRIFTASMRRGFWSKAIAYFAWAITAL
YILGWLRPLTGWLNSVTFDFGESEVSVLRILISLALAAFAIWLGRLIGNVAQSQLRGSPELPASVGGLLGQSIKIGVMIV
AVLIALNFLGVNVAALTFFSGALGVGIGFGLQSVFSNFISGVIILMEKSLKVGDFIELQSGLSGVVKEINFRSTVVMTND
NVDIIVPNEAFIKAQLINWTMTEARRRIHVPFGVAYGTDKALVEKAGLEAAASVPWTFDDRGEHTPQVWLVKFGDFRLEF
ELIVWLTDEAVLRPQKVMADYVWAVHSAIEKYEIHIPMPQRDLHFKSPGTLSVRIETGEAEKPAPDAEDAPEKASAEASE
TKS

Sequences:

>Translated_483_residues
MPLRIAASMSLDLLQPETAAPLIAELQESVTRLIEAARDELSSPDTLWQVGALILGAALGYIISRFPKTMLMQAAEKRGR
IDFVLLTYHSLSRIVWPVFTLILLAAATAAFEAVGLGNATLHIATSLLLAWIVVRIFTASMRRGFWSKAIAYFAWAITAL
YILGWLRPLTGWLNSVTFDFGESEVSVLRILISLALAAFAIWLGRLIGNVAQSQLRGSPELPASVGGLLGQSIKIGVMIV
AVLIALNFLGVNVAALTFFSGALGVGIGFGLQSVFSNFISGVIILMEKSLKVGDFIELQSGLSGVVKEINFRSTVVMTND
NVDIIVPNEAFIKAQLINWTMTEARRRIHVPFGVAYGTDKALVEKAGLEAAASVPWTFDDRGEHTPQVWLVKFGDFRLEF
ELIVWLTDEAVLRPQKVMADYVWAVHSAIEKYEIHIPMPQRDLHFKSPGTLSVRIETGEAEKPAPDAEDAPEKASAEASE
TKS
>Mature_482_residues
PLRIAASMSLDLLQPETAAPLIAELQESVTRLIEAARDELSSPDTLWQVGALILGAALGYIISRFPKTMLMQAAEKRGRI
DFVLLTYHSLSRIVWPVFTLILLAAATAAFEAVGLGNATLHIATSLLLAWIVVRIFTASMRRGFWSKAIAYFAWAITALY
ILGWLRPLTGWLNSVTFDFGESEVSVLRILISLALAAFAIWLGRLIGNVAQSQLRGSPELPASVGGLLGQSIKIGVMIVA
VLIALNFLGVNVAALTFFSGALGVGIGFGLQSVFSNFISGVIILMEKSLKVGDFIELQSGLSGVVKEINFRSTVVMTNDN
VDIIVPNEAFIKAQLINWTMTEARRRIHVPFGVAYGTDKALVEKAGLEAAASVPWTFDDRGEHTPQVWLVKFGDFRLEFE
LIVWLTDEAVLRPQKVMADYVWAVHSAIEKYEIHIPMPQRDLHFKSPGTLSVRIETGEAEKPAPDAEDAPEKASAEASET
KS

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=212, Percent_Identity=33.4905660377358, Blast_Score=125, Evalue=9e-30,
Organism=Escherichia coli, GI2367355, Length=172, Percent_Identity=35.4651162790698, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1789291, Length=259, Percent_Identity=28.5714285714286, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1787591, Length=169, Percent_Identity=28.9940828402367, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 52734; Mature: 52603

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLRIAASMSLDLLQPETAAPLIAELQESVTRLIEAARDELSSPDTLWQVGALILGAALG
CCEEEEECCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
YIISRFPKTMLMQAAEKRGRIDFVLLTYHSLSRIVWPVFTLILLAAATAAFEAVGLGNAT
HHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
LHIATSLLLAWIVVRIFTASMRRGFWSKAIAYFAWAITALYILGWLRPLTGWLNSVTFDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GESEVSVLRILISLALAAFAIWLGRLIGNVAQSQLRGSPELPASVGGLLGQSIKIGVMIV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHH
AVLIALNFLGVNVAALTFFSGALGVGIGFGLQSVFSNFISGVIILMEKSLKVGDFIELQS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
GLSGVVKEINFRSTVVMTNDNVDIIVPNEAFIKAQLINWTMTEARRRIHVPFGVAYGTDK
HHHHHHHHCCCCEEEEEECCCEEEEECCHHHEEHHHHHHHHHHHHCEEECCEEEEECCCH
ALVEKAGLEAAASVPWTFDDRGEHTPQVWLVKFGDFRLEFELIVWLTDEAVLRPQKVMAD
HHHHHHCCHHHCCCCCCCCCCCCCCCEEEEEEECCEEEEEEEEEEECCHHHHCHHHHHHH
YVWAVHSAIEKYEIHIPMPQRDLHFKSPGTLSVRIETGEAEKPAPDAEDAPEKASAEASE
HHHHHHHHHHHEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHC
TKS
CCC
>Mature Secondary Structure 
PLRIAASMSLDLLQPETAAPLIAELQESVTRLIEAARDELSSPDTLWQVGALILGAALG
CEEEEECCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
YIISRFPKTMLMQAAEKRGRIDFVLLTYHSLSRIVWPVFTLILLAAATAAFEAVGLGNAT
HHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
LHIATSLLLAWIVVRIFTASMRRGFWSKAIAYFAWAITALYILGWLRPLTGWLNSVTFDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GESEVSVLRILISLALAAFAIWLGRLIGNVAQSQLRGSPELPASVGGLLGQSIKIGVMIV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHH
AVLIALNFLGVNVAALTFFSGALGVGIGFGLQSVFSNFISGVIILMEKSLKVGDFIELQS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
GLSGVVKEINFRSTVVMTNDNVDIIVPNEAFIKAQLINWTMTEARRRIHVPFGVAYGTDK
HHHHHHHHCCCCEEEEEECCCEEEEECCHHHEEHHHHHHHHHHHHCEEECCEEEEECCCH
ALVEKAGLEAAASVPWTFDDRGEHTPQVWLVKFGDFRLEFELIVWLTDEAVLRPQKVMAD
HHHHHHCCHHHCCCCCCCCCCCCCCCEEEEEEECCEEEEEEEEEEECCHHHHCHHHHHHH
YVWAVHSAIEKYEIHIPMPQRDLHFKSPGTLSVRIETGEAEKPAPDAEDAPEKASAEASE
HHHHHHHHHHHEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHC
TKS
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]