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The map label for this gene is rlmH

Identifier: 114331598

GI number: 114331598

Start: 1694877

End: 1695344

Strand: Direct

Name: rlmH

Synonym: Neut_1612

Alternate gene names: 114331598

Gene position: 1694877-1695344 (Clockwise)

Preceding gene: 114331597

Following gene: 114331599

Centisome position: 63.69

GC content: 48.72

Gene sequence:

>468_bases
ATGAAGCTCCATATCCTGGCGGTTGGCAATAAAATGCCGGACTGGATTAAAAAAGGATACACAGAATACAGCCAGCGCAT
GCCTAAAGAAGCCGAGATGCAGCTGGTAGAAATAAAACCGGAAAAGCGGATCGGTAAAAAGACCGAACAACTATTGCAAG
CCGAAAGTGAACGTATCCGTATCGCTTTACCGCCCGGGTGCCATATTGTTGTTCTGGATGAATCAGGCAAGCAAGCCACC
ACTGTCAGGCTGGCTGAACTGATGACCAATTGGAGAGAATCTGGCCGGGATGTAACGTTCATCATTGGCGGAGCAGATGG
ACTGCACCGGGATATTAAACAAATAGCTCACGAAAAACTAGCGCTTTCTACTATGACATTACCGCACGGGCTGGCGAGAG
TATTGCTGGCAGAACAACTTTACCGGGCATTTTCCATCACCCGGAATCACCCCTACCACCGTGCATAA

Upstream 100 bases:

>100_bases
TACGGTTAACTCATGGCTGCGAAAATTGGTTTTAAAATAACGTAGCCTCTTCCTAAACCTGTTCATTCCTATAAATTTCG
CACGCTTATTATTCTGCATC

Downstream 100 bases:

>100_bases
TTTGTAATCCTGCTGTAGAGTTCCAGCTACAAGTGCACAATATTTTGCTTATGGAAACATACCTTAAGGCTTGATCCGGG
AATGATTACTACAGCTGGCA

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MKLHILAVGNKMPDWIKKGYTEYSQRMPKEAEMQLVEIKPEKRIGKKTEQLLQAESERIRIALPPGCHIVVLDESGKQAT
TVRLAELMTNWRESGRDVTFIIGGADGLHRDIKQIAHEKLALSTMTLPHGLARVLLAEQLYRAFSITRNHPYHRA

Sequences:

>Translated_155_residues
MKLHILAVGNKMPDWIKKGYTEYSQRMPKEAEMQLVEIKPEKRIGKKTEQLLQAESERIRIALPPGCHIVVLDESGKQAT
TVRLAELMTNWRESGRDVTFIIGGADGLHRDIKQIAHEKLALSTMTLPHGLARVLLAEQLYRAFSITRNHPYHRA
>Mature_155_residues
MKLHILAVGNKMPDWIKKGYTEYSQRMPKEAEMQLVEIKPEKRIGKKTEQLLQAESERIRIALPPGCHIVVLDESGKQAT
TVRLAELMTNWRESGRDVTFIIGGADGLHRDIKQIAHEKLALSTMTLPHGLARVLLAEQLYRAFSITRNHPYHRA

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=156, Percent_Identity=48.0769230769231, Blast_Score=142, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_NITEC (Q0AFM7)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_747820.1
- ProteinModelPortal:   Q0AFM7
- SMR:   Q0AFM7
- STRING:   Q0AFM7
- GeneID:   4272100
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_1612
- NMPDR:   fig|335283.3.peg.1914
- eggNOG:   COG1576
- HOGENOM:   HBG622807
- OMA:   TANHPYH
- PhylomeDB:   Q0AFM7
- ProtClustDB:   PRK00103
- BioCyc:   NEUT335283:NEUT_1612-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR016051
- InterPro:   IPR003742
- PIRSF:   PIRSF004505
- TIGRFAMs:   TIGR00246

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 17676; Mature: 17676

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLHILAVGNKMPDWIKKGYTEYSQRMPKEAEMQLVEIKPEKRIGKKTEQLLQAESERIR
CEEEEEEECCCCHHHHHHHHHHHHHHCCCHHCEEEEEECCHHHHHHHHHHHHHHCCCEEE
IALPPGCHIVVLDESGKQATTVRLAELMTNWRESGRDVTFIIGGADGLHRDIKQIAHEKL
EEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHH
ALSTMTLPHGLARVLLAEQLYRAFSITRNHPYHRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKLHILAVGNKMPDWIKKGYTEYSQRMPKEAEMQLVEIKPEKRIGKKTEQLLQAESERIR
CEEEEEEECCCCHHHHHHHHHHHHHHCCCHHCEEEEEECCHHHHHHHHHHHHHHCCCEEE
IALPPGCHIVVLDESGKQATTVRLAELMTNWRESGRDVTFIIGGADGLHRDIKQIAHEKL
EEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHH
ALSTMTLPHGLARVLLAEQLYRAFSITRNHPYHRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA