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The map label for this gene is aceF [H]

Identifier: 114331595

GI number: 114331595

Start: 1692325

End: 1693674

Strand: Direct

Name: aceF [H]

Synonym: Neut_1609

Alternate gene names: 114331595

Gene position: 1692325-1693674 (Clockwise)

Preceding gene: 114331594

Following gene: 114331596

Centisome position: 63.6

GC content: 47.93

Gene sequence:

>1350_bases
GTGGCTGAAGTCAAGAAAATTCTGGTCCCGGATATCGGGAGTTTTGAAGATGTCCCGGTTATCGAAATCGTGGTCAAGCC
TGGCGATAGCGTACAGGCTGAAGATCCTTTAATCGTGTTGGAATCAGACAAGGCCACGGTTGAGGTGCCCTCGCCTTACT
CCGGAATAATCCGAGAAATTATGCTTCAGCCAGGCACTAAGGTCTCTAAGGATTCAGAAATCCTGACTATGGAAGTGGTA
TCAAATGCTGAAGAAAAGGTCTCCAAACCTCAGCCATCCGGGTCTCCATCCGAACCACAACCCGCACGAACAGAAGCAGC
AGCCGAACCAAATCAGCCTGAAACTATACCGGTGGTCAAATCTACACCCACCACAGCAGCGAAACCAGCGACCCCTCCTT
CTTCATTGCCGAAACTTGATCAGGAAGTCGGAGCGCATGGTAAGACAATGCCTCATGCAAGTCCCTCGATAAGACGATTT
GCCCGGGAATTGGGAGTGGATCTGACCAGAGTAGTTGGAACCGGCCCCAAGCTGCGTATTCTCAAGGAAGATGTACTGGC
TTTCGTAAAACAGGTATTAGCTAGCGAACACAATACTGGAAGTGTGCTTAACCTACTGCCCTGGCCATATGTAGATTTTG
CGAAATTTGGGCCAATCGAACTGAAATCATTGTCACGTATCCGACAAATTTCAGGTGCTAACCTTCATCGCAATTGGGTC
ATGATTCCGCATGTCACGCAGTTCGACGAGGCAGATATCACCGATCTGGAAGCGCTCAGAAAAACTCATAATGAAACCCG
GCAGAATAACGGCACCAAACTAACTATATTAGCTTTTCTTATCAAGGCGGTTACAGCAGCTCTGAAAAAATTCCCAGAAT
TTAACGCTTCCCTGGACAACAGTACAGAGGAAAGTCAACTCATCATAAAATATTACTACCATTTGGGATTTGCAGTCGAT
ACACCCAACGGGCTGGTGGTTCCAGTTATTCGGGATGCCGATCAGAAAGGAGTAATCGATATCGCTCAGGAACTTGCCAG
GCTATCCTCACTTGCCCGTAAAGGTAAATTAATGCCCAGTGACATGCAAGGTGCAAGCTTTACTATATCGAGCCTGGGAG
GTATCGGTGGAACCGGCTTTACACCCATTATCAATGCGCCGGAAGTAGCCATTCTAGGGATATCCCGTGCAGGTCTGAAA
CCAGTTTATCAGAATGAAAAATTTGTTCCACGCCTGATACTCCCGTTCTCGATATCCTATGATCACCGGGTAATTGATGG
TGCAGCGGCCGCACGTTTCACTACACATTTGGCCAGCATTCTGACAGACATGCGACTTGCGCTGTTCTGA

Upstream 100 bases:

>100_bases
GGAAAAACCCGAACCGGTCAAGGTTTAAGCCCGTTCACAATTGGTAGAATAATTTTGTCTCACCCTGACGATATTTGTTG
CAGCGAATCAAGGAGCACAT

Downstream 100 bases:

>100_bases
GTGATGCACAACCAATCAGGCAATGCGGTGCCTGATTGTCTATTTATCGGCAAATGGCTGAAGTAACAGCATACTCACTC
ATCGGAATTTACGGTGGAAC

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MAEVKKILVPDIGSFEDVPVIEIVVKPGDSVQAEDPLIVLESDKATVEVPSPYSGIIREIMLQPGTKVSKDSEILTMEVV
SNAEEKVSKPQPSGSPSEPQPARTEAAAEPNQPETIPVVKSTPTTAAKPATPPSSLPKLDQEVGAHGKTMPHASPSIRRF
ARELGVDLTRVVGTGPKLRILKEDVLAFVKQVLASEHNTGSVLNLLPWPYVDFAKFGPIELKSLSRIRQISGANLHRNWV
MIPHVTQFDEADITDLEALRKTHNETRQNNGTKLTILAFLIKAVTAALKKFPEFNASLDNSTEESQLIIKYYYHLGFAVD
TPNGLVVPVIRDADQKGVIDIAQELARLSSLARKGKLMPSDMQGASFTISSLGGIGGTGFTPIINAPEVAILGISRAGLK
PVYQNEKFVPRLILPFSISYDHRVIDGAAAARFTTHLASILTDMRLALF

Sequences:

>Translated_449_residues
MAEVKKILVPDIGSFEDVPVIEIVVKPGDSVQAEDPLIVLESDKATVEVPSPYSGIIREIMLQPGTKVSKDSEILTMEVV
SNAEEKVSKPQPSGSPSEPQPARTEAAAEPNQPETIPVVKSTPTTAAKPATPPSSLPKLDQEVGAHGKTMPHASPSIRRF
ARELGVDLTRVVGTGPKLRILKEDVLAFVKQVLASEHNTGSVLNLLPWPYVDFAKFGPIELKSLSRIRQISGANLHRNWV
MIPHVTQFDEADITDLEALRKTHNETRQNNGTKLTILAFLIKAVTAALKKFPEFNASLDNSTEESQLIIKYYYHLGFAVD
TPNGLVVPVIRDADQKGVIDIAQELARLSSLARKGKLMPSDMQGASFTISSLGGIGGTGFTPIINAPEVAILGISRAGLK
PVYQNEKFVPRLILPFSISYDHRVIDGAAAARFTTHLASILTDMRLALF
>Mature_448_residues
AEVKKILVPDIGSFEDVPVIEIVVKPGDSVQAEDPLIVLESDKATVEVPSPYSGIIREIMLQPGTKVSKDSEILTMEVVS
NAEEKVSKPQPSGSPSEPQPARTEAAAEPNQPETIPVVKSTPTTAAKPATPPSSLPKLDQEVGAHGKTMPHASPSIRRFA
RELGVDLTRVVGTGPKLRILKEDVLAFVKQVLASEHNTGSVLNLLPWPYVDFAKFGPIELKSLSRIRQISGANLHRNWVM
IPHVTQFDEADITDLEALRKTHNETRQNNGTKLTILAFLIKAVTAALKKFPEFNASLDNSTEESQLIIKYYYHLGFAVDT
PNGLVVPVIRDADQKGVIDIAQELARLSSLARKGKLMPSDMQGASFTISSLGGIGGTGFTPIINAPEVAILGISRAGLKP
VYQNEKFVPRLILPFSISYDHRVIDGAAAARFTTHLASILTDMRLALF

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=471, Percent_Identity=29.9363057324841, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI31711992, Length=435, Percent_Identity=30.1149425287356, Blast_Score=142, Evalue=9e-34,
Organism=Homo sapiens, GI19923748, Length=230, Percent_Identity=34.7826086956522, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI203098816, Length=478, Percent_Identity=28.0334728033473, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI203098753, Length=478, Percent_Identity=28.0334728033473, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI260898739, Length=173, Percent_Identity=35.8381502890173, Blast_Score=96, Evalue=8e-20,
Organism=Escherichia coli, GI1786305, Length=301, Percent_Identity=54.8172757475083, Blast_Score=342, Evalue=2e-95,
Organism=Escherichia coli, GI1786946, Length=439, Percent_Identity=29.1571753986333, Blast_Score=166, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17537937, Length=431, Percent_Identity=31.0904872389791, Blast_Score=191, Evalue=8e-49,
Organism=Caenorhabditis elegans, GI25146366, Length=435, Percent_Identity=29.8850574712644, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17560088, Length=440, Percent_Identity=29.7727272727273, Blast_Score=143, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17538894, Length=316, Percent_Identity=31.0126582278481, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6320352, Length=428, Percent_Identity=29.6728971962617, Blast_Score=160, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6324258, Length=437, Percent_Identity=27.6887871853547, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI18859875, Length=442, Percent_Identity=32.3529411764706, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24645909, Length=211, Percent_Identity=33.175355450237, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 48707; Mature: 48575

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEVKKILVPDIGSFEDVPVIEIVVKPGDSVQAEDPLIVLESDKATVEVPSPYSGIIREI
CCCCHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCEEECCCCHHHHHHHH
MLQPGTKVSKDSEILTMEVVSNAEEKVSKPQPSGSPSEPQPARTEAAAEPNQPETIPVVK
HHCCCCCCCCCCCEEEHHHHHCHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCEEEEEE
STPTTAAKPATPPSSLPKLDQEVGAHGKTMPHASPSIRRFARELGVDLTRVVGTGPKLRI
CCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCEEH
LKEDVLAFVKQVLASEHNTGSVLNLLPWPYVDFAKFGPIELKSLSRIRQISGANLHRNWV
HHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHCCCCHHHHHHHHHHHCCCCCCCCEE
MIPHVTQFDEADITDLEALRKTHNETRQNNGTKLTILAFLIKAVTAALKKFPEFNASLDN
EECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCC
STEESQLIIKYYYHLGFAVDTPNGLVVPVIRDADQKGVIDIAQELARLSSLARKGKLMPS
CCCHHHHHHEEEEHHCEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
DMQGASFTISSLGGIGGTGFTPIINAPEVAILGISRAGLKPVYQNEKFVPRLILPFSISY
CCCCCEEEHHHCCCCCCCCCCCCCCCCCEEEEECCHHCCCCCCCCCCCCCEEEEEEECCC
DHRVIDGAAAARFTTHLASILTDMRLALF
CCEEHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AEVKKILVPDIGSFEDVPVIEIVVKPGDSVQAEDPLIVLESDKATVEVPSPYSGIIREI
CCCHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCEEECCCCHHHHHHHH
MLQPGTKVSKDSEILTMEVVSNAEEKVSKPQPSGSPSEPQPARTEAAAEPNQPETIPVVK
HHCCCCCCCCCCCEEEHHHHHCHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCEEEEEE
STPTTAAKPATPPSSLPKLDQEVGAHGKTMPHASPSIRRFARELGVDLTRVVGTGPKLRI
CCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCEEH
LKEDVLAFVKQVLASEHNTGSVLNLLPWPYVDFAKFGPIELKSLSRIRQISGANLHRNWV
HHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHCCCCHHHHHHHHHHHCCCCCCCCEE
MIPHVTQFDEADITDLEALRKTHNETRQNNGTKLTILAFLIKAVTAALKKFPEFNASLDN
EECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCC
STEESQLIIKYYYHLGFAVDTPNGLVVPVIRDADQKGVIDIAQELARLSSLARKGKLMPS
CCCHHHHHHEEEEHHCEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
DMQGASFTISSLGGIGGTGFTPIINAPEVAILGISRAGLKPVYQNEKFVPRLILPFSISY
CCCCCEEEHHHCCCCCCCCCCCCCCCCCEEEEECCHHCCCCCCCCCCCCCEEEEEEECCC
DHRVIDGAAAARFTTHLASILTDMRLALF
CCEEHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]