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The map label for this gene is spoT [H]

Identifier: 114331587

GI number: 114331587

Start: 1681519

End: 1683687

Strand: Direct

Name: spoT [H]

Synonym: Neut_1601

Alternate gene names: 114331587

Gene position: 1681519-1683687 (Clockwise)

Preceding gene: 114331585

Following gene: 114331588

Centisome position: 63.19

GC content: 45.83

Gene sequence:

>2169_bases
ATGTCAGAAGCTATTTTGTATGACTTAAATGACTCCGCTGTTATTACATCAAAAGCGGGGCTGCTTTTTGACGAGGTATC
TCAATATCTCAAGTCTGAAGATATGGTTTTACTTAAAAATGCTTATTTCTTCAGTCAGGAAGCACACTCCGGCCAATTCA
GAAAATCCGGCGAACCTTATATTTCTCATCCTGTTGCAGTCGCCAGAATACTGGGTGAACTCCATCTGGATACGACAACG
CTCGCTGCCGCTCTGCTGCACGATGTGGTGGAGGATACCGGTATCCTGAAGGAAGCAATCAGCAAACGATTTGGTTCTTC
CGTAGCTGAGCTTGTAGATGGCGTTTCCAAGCTCGATCGGATCAAGTTCCAAACTCAAGCAGATATGCAAGTGGAAAATT
TCCGCAAGATGTTGCTGGCCATGGCACAAGATATACGAGTCATATTGATCAAGCTGGCCGATCGTCTGCACAACATGCGC
ACACTTGAGGTAATGCCACAAGAGAAACAGCTCCGTATTGCACGAGAAACGCTTGAGATCTACGCGCCGATTGCACATCG
CCTTGGCCTGGAGAATATCTATCAGGAACTGCAGGAGCTGGGGTTTTGTTTTTCTTATCCAACCCGCTATAAGGTTCTGC
TCAAGGCAATCAAGGCAGCAAGAGGCAATCGCCGGGAGGTAGTTGGAAAAATCCTGGAAGCAACCAAACAGCGCTTACAG
GAAGCCGGGCTGGATGCCATGGTAACCGGCCGCGAGAAACATTTGTACAGTATCTACAAGAAAATGGCAGGGAAACATCT
TCGTTTCTCGGATGTACTGGATATTTATGGTTTTCGAGTGGTTGTTAAAGATGTTTCATCTTGCTACGTGGCGCTTGGAG
CCCTCCACAGCCTATACAAACCCATCCCTGGAAAGTTCAAAGATTACATTGCCATCCCCAAACCAAACGGCTATCAATCA
TTACACAGCACATTACTTGGGCCTTATGGATTGCCGATAGAAATCCAGATCCGCACCCAAGAAATGCACCATATTGCTGA
GGCCGGAGTTGCTTCACACTGGCTTTACAAGAACAAGGGCAGTGACACTGCAATGGATGATTTGTACATGAAAGCCAATC
AGTGGATGAAAGGTTTGCTGGAGACCTTGAATGACTCATCCGATTCGCTGGAATTTCTGGAGCATCTCAAAGTTGATCTA
TTCCCAGGAGAAATATATGTTTTTACTCCGCAAGGCAAGATATTAACGTTACCACGAGGCGCAACCATAGTAGATTTTGC
TTATGCTGTGCATACTGATATCGGGAATTGCTGCGTAGCAGCCAGAATCAACGGTGAAATCACACCGCTTCGCACCCGCC
TGAGAAGTGGAGATCGCGTGGAAATTATTACTGCACCTGCAGCCAAACCAAATCCTGTTTGGCTATCCTATGTTGCAACT
GGCAGAGCACGTTCCAGTATTCGCTATTTTCTGAGAACCATTCAATACGATGAATCGATCAGGCTTGGAGAACGCCTGTT
GAATCAGGCATTGCATTCCTTTGGTACGAGCGCGGAAGCTATCGAGCCTTCACAATGGGAAAAACTGGCAAGAGAGAGCG
GGGCTAAATCCAAGGAAGCACTTTTGGCAGATATTGCTCTGGGAAAACAGCTTGCTGCAGTTATAGCTAAACGTCTCGCT
ACCCCGTCAGGTGAATCTATATCAAATATTGAAGGCAACAACTCTATCACCATACTCGGAACCGAGGGAATGGCAGTCAA
ATTCGCACGATGCTGCTACCCTATACCCGGTGACGGCATCGTTGGATTGATTAAAAAAGATCAGGGGCTGGTGATTCATA
CACAAGATTGCTCTGCAGTAATCAGAATCAAGGATCATAAAAACATAGACAATCAACTGGACGTTATCTGGGGAACGAAT
ATTGACAGAACCTTTCCGGTCAATATATTCATGACGGTAGTAAATAAAAGCGGGGTATTGGCCAGAGTAACCGCAGAGAT
TGCTAAAGCTGACTCAAATATTGACGATATAACGTTGGAAAATGACAAAGATTACACCACGATGCGCTTTATCCTGCAGG
CCAGGGATCGTCAACATCTTGCACAAATTTTCCGCAGACTGAAGCATATCGGTGAAATAGTCAAAATGGGGCGCATCAGA
AACCAATAA

Upstream 100 bases:

>100_bases
TGCGGGCTTGAAATTAATCCTTTGGATTAGAAATAGTCCTCACAGCACAGCGGTTCACGGCCGACAAAAATCATTTCATA
CATAAAAATTTTAAAATTTC

Downstream 100 bases:

>100_bases
ATTAATTACTCTATAAAAATGAGTGCTGATAACCTGTTGCTTGATTTCACCTCACCCGGGGCAATCCCCCCCGATCCTGT
CGAGGAGATTCAGCGAATAG

Product: (p)ppGpp synthetase I, SpoT/RelA

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 722; Mature: 721

Protein sequence:

>722_residues
MSEAILYDLNDSAVITSKAGLLFDEVSQYLKSEDMVLLKNAYFFSQEAHSGQFRKSGEPYISHPVAVARILGELHLDTTT
LAAALLHDVVEDTGILKEAISKRFGSSVAELVDGVSKLDRIKFQTQADMQVENFRKMLLAMAQDIRVILIKLADRLHNMR
TLEVMPQEKQLRIARETLEIYAPIAHRLGLENIYQELQELGFCFSYPTRYKVLLKAIKAARGNRREVVGKILEATKQRLQ
EAGLDAMVTGREKHLYSIYKKMAGKHLRFSDVLDIYGFRVVVKDVSSCYVALGALHSLYKPIPGKFKDYIAIPKPNGYQS
LHSTLLGPYGLPIEIQIRTQEMHHIAEAGVASHWLYKNKGSDTAMDDLYMKANQWMKGLLETLNDSSDSLEFLEHLKVDL
FPGEIYVFTPQGKILTLPRGATIVDFAYAVHTDIGNCCVAARINGEITPLRTRLRSGDRVEIITAPAAKPNPVWLSYVAT
GRARSSIRYFLRTIQYDESIRLGERLLNQALHSFGTSAEAIEPSQWEKLARESGAKSKEALLADIALGKQLAAVIAKRLA
TPSGESISNIEGNNSITILGTEGMAVKFARCCYPIPGDGIVGLIKKDQGLVIHTQDCSAVIRIKDHKNIDNQLDVIWGTN
IDRTFPVNIFMTVVNKSGVLARVTAEIAKADSNIDDITLENDKDYTTMRFILQARDRQHLAQIFRRLKHIGEIVKMGRIR
NQ

Sequences:

>Translated_722_residues
MSEAILYDLNDSAVITSKAGLLFDEVSQYLKSEDMVLLKNAYFFSQEAHSGQFRKSGEPYISHPVAVARILGELHLDTTT
LAAALLHDVVEDTGILKEAISKRFGSSVAELVDGVSKLDRIKFQTQADMQVENFRKMLLAMAQDIRVILIKLADRLHNMR
TLEVMPQEKQLRIARETLEIYAPIAHRLGLENIYQELQELGFCFSYPTRYKVLLKAIKAARGNRREVVGKILEATKQRLQ
EAGLDAMVTGREKHLYSIYKKMAGKHLRFSDVLDIYGFRVVVKDVSSCYVALGALHSLYKPIPGKFKDYIAIPKPNGYQS
LHSTLLGPYGLPIEIQIRTQEMHHIAEAGVASHWLYKNKGSDTAMDDLYMKANQWMKGLLETLNDSSDSLEFLEHLKVDL
FPGEIYVFTPQGKILTLPRGATIVDFAYAVHTDIGNCCVAARINGEITPLRTRLRSGDRVEIITAPAAKPNPVWLSYVAT
GRARSSIRYFLRTIQYDESIRLGERLLNQALHSFGTSAEAIEPSQWEKLARESGAKSKEALLADIALGKQLAAVIAKRLA
TPSGESISNIEGNNSITILGTEGMAVKFARCCYPIPGDGIVGLIKKDQGLVIHTQDCSAVIRIKDHKNIDNQLDVIWGTN
IDRTFPVNIFMTVVNKSGVLARVTAEIAKADSNIDDITLENDKDYTTMRFILQARDRQHLAQIFRRLKHIGEIVKMGRIR
NQ
>Mature_721_residues
SEAILYDLNDSAVITSKAGLLFDEVSQYLKSEDMVLLKNAYFFSQEAHSGQFRKSGEPYISHPVAVARILGELHLDTTTL
AAALLHDVVEDTGILKEAISKRFGSSVAELVDGVSKLDRIKFQTQADMQVENFRKMLLAMAQDIRVILIKLADRLHNMRT
LEVMPQEKQLRIARETLEIYAPIAHRLGLENIYQELQELGFCFSYPTRYKVLLKAIKAARGNRREVVGKILEATKQRLQE
AGLDAMVTGREKHLYSIYKKMAGKHLRFSDVLDIYGFRVVVKDVSSCYVALGALHSLYKPIPGKFKDYIAIPKPNGYQSL
HSTLLGPYGLPIEIQIRTQEMHHIAEAGVASHWLYKNKGSDTAMDDLYMKANQWMKGLLETLNDSSDSLEFLEHLKVDLF
PGEIYVFTPQGKILTLPRGATIVDFAYAVHTDIGNCCVAARINGEITPLRTRLRSGDRVEIITAPAAKPNPVWLSYVATG
RARSSIRYFLRTIQYDESIRLGERLLNQALHSFGTSAEAIEPSQWEKLARESGAKSKEALLADIALGKQLAAVIAKRLAT
PSGESISNIEGNNSITILGTEGMAVKFARCCYPIPGDGIVGLIKKDQGLVIHTQDCSAVIRIKDHKNIDNQLDVIWGTNI
DRTFPVNIFMTVVNKSGVLARVTAEIAKADSNIDDITLENDKDYTTMRFILQARDRQHLAQIFRRLKHIGEIVKMGRIRN
Q

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance [H]

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=135, Percent_Identity=38.5185185185185, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1790082, Length=708, Percent_Identity=49.1525423728814, Blast_Score=687, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=687, Percent_Identity=31.5866084425036, Blast_Score=399, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25143118, Length=133, Percent_Identity=37.593984962406, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24650996, Length=143, Percent_Identity=37.0629370629371, Blast_Score=70, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 80842; Mature: 80710

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAILYDLNDSAVITSKAGLLFDEVSQYLKSEDMVLLKNAYFFSQEAHSGQFRKSGEPY
CCCCEEEECCCCEEEECCCCHHHHHHHHHHCCCCEEEEECHHHCCCCCCCCCCCCCCCCC
ISHPVAVARILGELHLDTTTLAAALLHDVVEDTGILKEAISKRFGSSVAELVDGVSKLDR
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
IKFQTQADMQVENFRKMLLAMAQDIRVILIKLADRLHNMRTLEVMPQEKQLRIARETLEI
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHH
YAPIAHRLGLENIYQELQELGFCFSYPTRYKVLLKAIKAARGNRREVVGKILEATKQRLQ
HHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
EAGLDAMVTGREKHLYSIYKKMAGKHLRFSDVLDIYGFRVVVKDVSSCYVALGALHSLYK
HCCCCEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PIPGKFKDYIAIPKPNGYQSLHSTLLGPYGLPIEIQIRTQEMHHIAEAGVASHWLYKNKG
CCCCCCCCEEECCCCCCHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHEECCCC
SDTAMDDLYMKANQWMKGLLETLNDSSDSLEFLEHLKVDLFPGEIYVFTPQGKILTLPRG
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEECCEEEEECCCCCEEECCCC
ATIVDFAYAVHTDIGNCCVAARINGEITPLRTRLRSGDRVEIITAPAAKPNPVWLSYVAT
CCHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEEC
GRARSSIRYFLRTIQYDESIRLGERLLNQALHSFGTSAEAIEPSQWEKLARESGAKSKEA
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHH
LLADIALGKQLAAVIAKRLATPSGESISNIEGNNSITILGTEGMAVKFARCCYPIPGDGI
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHCCCCCCCCE
VGLIKKDQGLVIHTQDCSAVIRIKDHKNIDNQLDVIWGTNIDRTFPVNIFMTVVNKSGVL
EEEEECCCCEEEEECCCCEEEEEECCCCCCCCEEEEECCCCCCEECCEEEEEEECCCCCH
ARVTAEIAKADSNIDDITLENDKDYTTMRFILQARDRQHLAQIFRRLKHIGEIVKMGRIR
HHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NQ
CC
>Mature Secondary Structure 
SEAILYDLNDSAVITSKAGLLFDEVSQYLKSEDMVLLKNAYFFSQEAHSGQFRKSGEPY
CCCEEEECCCCEEEECCCCHHHHHHHHHHCCCCEEEEECHHHCCCCCCCCCCCCCCCCC
ISHPVAVARILGELHLDTTTLAAALLHDVVEDTGILKEAISKRFGSSVAELVDGVSKLDR
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
IKFQTQADMQVENFRKMLLAMAQDIRVILIKLADRLHNMRTLEVMPQEKQLRIARETLEI
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHH
YAPIAHRLGLENIYQELQELGFCFSYPTRYKVLLKAIKAARGNRREVVGKILEATKQRLQ
HHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
EAGLDAMVTGREKHLYSIYKKMAGKHLRFSDVLDIYGFRVVVKDVSSCYVALGALHSLYK
HCCCCEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PIPGKFKDYIAIPKPNGYQSLHSTLLGPYGLPIEIQIRTQEMHHIAEAGVASHWLYKNKG
CCCCCCCCEEECCCCCCHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHEECCCC
SDTAMDDLYMKANQWMKGLLETLNDSSDSLEFLEHLKVDLFPGEIYVFTPQGKILTLPRG
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEECCEEEEECCCCCEEECCCC
ATIVDFAYAVHTDIGNCCVAARINGEITPLRTRLRSGDRVEIITAPAAKPNPVWLSYVAT
CCHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEEC
GRARSSIRYFLRTIQYDESIRLGERLLNQALHSFGTSAEAIEPSQWEKLARESGAKSKEA
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHH
LLADIALGKQLAAVIAKRLATPSGESISNIEGNNSITILGTEGMAVKFARCCYPIPGDGI
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHCCCCCCCCE
VGLIKKDQGLVIHTQDCSAVIRIKDHKNIDNQLDVIWGTNIDRTFPVNIFMTVVNKSGVL
EEEEECCCCEEEEECCCCEEEEEECCCCCCCCEEEEECCCCCCEECCEEEEEEECCCCCH
ARVTAEIAKADSNIDDITLENDKDYTTMRFILQARDRQHLAQIFRRLKHIGEIVKMGRIR
HHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NQ
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]