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The map label for this gene is ispD

Identifier: 114331512

GI number: 114331512

Start: 1595719

End: 1596420

Strand: Reverse

Name: ispD

Synonym: Neut_1525

Alternate gene names: 114331512

Gene position: 1596420-1595719 (Counterclockwise)

Preceding gene: 114331514

Following gene: 114331509

Centisome position: 59.99

GC content: 51.85

Gene sequence:

>702_bases
ATGGTTAAATTTATTGCGCTGATTACAGCTGCCGGTTCCGGTTCGCGTATGGGAGAAGACATCCCCAAACAGTACCGGCC
ACTAGCAGGTAAACCTATGATTTACCACGCACTGCGTACCTTGTGTGGCATTGCCCGTATTTCCACAGTCTGCATTGTGC
TGGCACCTGAAGATACTGAATGGATTCGGCATAACTGGCGTGAATTTGCCGGCAAGATCCAGATATTTAACTGCGGTGGG
GCAACTCGTGCGGAAAGCGTTACCAATGGACTAAAGGCATTGCGTGCTGCAAATCATGTACAGGATCAGGATTGGATACT
AGTACACGATGCAGCGCGCCCGGGATTAAGTACAACCCTGGTTGAGCGGTTGCTTGACCAGCTTGCGGATGACGAAGTCG
GTGGTTTGCTGGCGGTGCCGCTGGCGGATACTTTAAAACGTGCGGATGATGCTGGTCGGGTAATTTGTACGGAACCGAGA
GAGCGGCTGTGGCAGGCGCAGACTCCACAAATGTTTCGCATGAAGCTGCTGCTTGAAGCGCTGGAAAAGGCCCCAGCGGG
TATCACGGATGATGCCAGTGCAGTAGAGGCATTGGGACTCTCTCCTAAGCTAGTAGTAGGAAATGCCTATAACTTCAAAG
TGACTTACCCGCAGGATTTGAAGCTGGCTGAGCTGATCCTGCGAGAACGTGCCATAACCTAA

Upstream 100 bases:

>100_bases
TTTTTCCTGTAACAAAGATCTGACAGTTTTCATAATAACCCTCCCGTTGATAAAATAACCGTATTTGATAATAAACAGGC
CCATTTTACAGGATTCTTGC

Downstream 100 bases:

>100_bases
AACCGATTCTGATCTGCGTTTGACATACTGGTAAAACGGTGTGTCAACGGGTCTGATTACCCCTCTTCTTAGAGATGTTT
AGTCCCACGGTGTGCTGGAA

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MVKFIALITAAGSGSRMGEDIPKQYRPLAGKPMIYHALRTLCGIARISTVCIVLAPEDTEWIRHNWREFAGKIQIFNCGG
ATRAESVTNGLKALRAANHVQDQDWILVHDAARPGLSTTLVERLLDQLADDEVGGLLAVPLADTLKRADDAGRVICTEPR
ERLWQAQTPQMFRMKLLLEALEKAPAGITDDASAVEALGLSPKLVVGNAYNFKVTYPQDLKLAELILRERAIT

Sequences:

>Translated_233_residues
MVKFIALITAAGSGSRMGEDIPKQYRPLAGKPMIYHALRTLCGIARISTVCIVLAPEDTEWIRHNWREFAGKIQIFNCGG
ATRAESVTNGLKALRAANHVQDQDWILVHDAARPGLSTTLVERLLDQLADDEVGGLLAVPLADTLKRADDAGRVICTEPR
ERLWQAQTPQMFRMKLLLEALEKAPAGITDDASAVEALGLSPKLVVGNAYNFKVTYPQDLKLAELILRERAIT
>Mature_233_residues
MVKFIALITAAGSGSRMGEDIPKQYRPLAGKPMIYHALRTLCGIARISTVCIVLAPEDTEWIRHNWREFAGKIQIFNCGG
ATRAESVTNGLKALRAANHVQDQDWILVHDAARPGLSTTLVERLLDQLADDEVGGLLAVPLADTLKRADDAGRVICTEPR
ERLWQAQTPQMFRMKLLLEALEKAPAGITDDASAVEALGLSPKLVVGNAYNFKVTYPQDLKLAELILRERAIT

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=235, Percent_Identity=27.6595744680851, Blast_Score=85, Evalue=6e-17,
Organism=Escherichia coli, GI1789104, Length=227, Percent_Identity=41.4096916299559, Blast_Score=143, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_NITEC (Q0AFW3)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_747734.1
- ProteinModelPortal:   Q0AFW3
- SMR:   Q0AFW3
- STRING:   Q0AFW3
- GeneID:   4273398
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_1525
- NMPDR:   fig|335283.3.peg.1807
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   FPQGAIL
- PhylomeDB:   Q0AFW3
- ProtClustDB:   PRK00155
- BioCyc:   NEUT335283:NEUT_1525-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 25596; Mature: 25596

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKFIALITAAGSGSRMGEDIPKQYRPLAGKPMIYHALRTLCGIARISTVCIVLAPEDTE
CEEEEEEEEECCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCH
WIRHNWREFAGKIQIFNCGGATRAESVTNGLKALRAANHVQDQDWILVHDAARPGLSTTL
HHHHHHHHHHCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHH
VERLLDQLADDEVGGLLAVPLADTLKRADDAGRVICTEPRERLWQAQTPQMFRMKLLLEA
HHHHHHHHCCCCCCCEEEECHHHHHHHCCCCCCEEECCHHHHHHCCCCHHHHHHHHHHHH
LEKAPAGITDDASAVEALGLSPKLVVGNAYNFKVTYPQDLKLAELILRERAIT
HHHCCCCCCCCHHHHHHHCCCCEEEECCCEEEEEECCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MVKFIALITAAGSGSRMGEDIPKQYRPLAGKPMIYHALRTLCGIARISTVCIVLAPEDTE
CEEEEEEEEECCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCH
WIRHNWREFAGKIQIFNCGGATRAESVTNGLKALRAANHVQDQDWILVHDAARPGLSTTL
HHHHHHHHHHCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHH
VERLLDQLADDEVGGLLAVPLADTLKRADDAGRVICTEPRERLWQAQTPQMFRMKLLLEA
HHHHHHHHCCCCCCCEEEECHHHHHHHCCCCCCEEECCHHHHHHCCCCHHHHHHHHHHHH
LEKAPAGITDDASAVEALGLSPKLVVGNAYNFKVTYPQDLKLAELILRERAIT
HHHCCCCCCCCHHHHHHHCCCCEEEECCCEEEEEECCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA