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The map label for this gene is rlmL [H]

Identifier: 114331500

GI number: 114331500

Start: 1583540

End: 1584724

Strand: Direct

Name: rlmL [H]

Synonym: Neut_1512

Alternate gene names: 114331500

Gene position: 1583540-1584724 (Clockwise)

Preceding gene: 114331499

Following gene: 114331501

Centisome position: 59.51

GC content: 50.3

Gene sequence:

>1185_bases
ATGACAGAACATTTCTTCGCGCCCTGTCCACGCGGGCTGGAAGCGGTTCTAGCGGCAGAACTTGAATGTCTTGAAGCGAC
CTCTATTGAGATTTTACCCGGAGGAGTAGCTTTTCGTGGAAGTTGGCTAACCTGTTACCGTGCCAATCTTGAAAGTCGCA
TCGCCAGCCGCATTTTGTGGCAAGTGGCCAGAAATCAGTATCGCAGTGAAGCCGACATTTACGACATCACACATCGCTTG
TCCTGGCCCGACTGGTTTGAGCCGCGATTGACAATAAAGGTCAATCTGGCCGCAATTAAATGCCCGCTGCGCAGTCTTGA
TTTCCTTACACTCCGAATCAAGGATGCAATCTGCGACAAATTCCGCACTATCCGTGGCAAGCGTCCAGACATCGACACCA
CCACACCCGATATACGTATTCATGGATTTCTTGATGCACGGCAATTTACCCTTTACCTGGATACTTCAGGCGAAGCACTA
TTCAAACGCGGTCTGCGCCAAACCCAGGGGGATGCTCCTTTGCGTGAAAACCTGGCTGCCGGCATCCTGGCATTGGCTGG
CTGGCATCCTGGCATTCCACTGCTTGACCCAATGTGTGGTAGCGGGACTCTGTTACTGGAAGCAGCACAAATTGCCTGCC
AGATTGCACCCGGCGCCAGGCGACAATTTGCATTCGAGAATCTCAAACTGTTTGATGCCAACTCCTGGAAAAAACTTAAA
CAAACGGCTGCAAACCGCCAACGTGAACGTATATTCCAATCCATTTATGGCAGTGATCTGTACGGCAATGCGCTGGTTAA
TGCACGTAACAATCTGGCAACAGCAGGATTAGCCGAGTGCGTCGCGCTGAAGCAGGCGAACGTTTTGGAAATTTCTCCTC
CAGCTGAAACCGGCATGTTGATCAGTAATCCACCGTACGGCGTACGCGTTGGAGATCACCAGACATTGGCCGAATTTTAT
CCCCAATTGGGAAATGTACTGAAACAACGATTCAGCGGCTGGCAGGCTTTTCTGCTAACTGCTGATCCCCTGCTGGCAAA
ATCTATTCGACTTACACCCTCACGCCGAACTCCACTTTTTAATGGCGCACTGGAATGTCGTTTGCTCGAATACAGACTGG
TTGCCGGCAGCATGCGCAGAGAGAAACCACCAAATGAATCAAGCAAAAACAGTCATAAACTTTAA

Upstream 100 bases:

>100_bases
TCTGTTTGCCTATGCTGTCTGGAGAAACAAACGGGGGTGAAATCACATTCCGGACTTCTACTACCAACCACTACAAGATT
GCAAAATAGCAGAAAATAAA

Downstream 100 bases:

>100_bases
GATAAAGTTATGAGAATAATCTTTCTACTGATTTTCTTGGCCTGTGCCGGATTAATTGGTTATGCGCTTTATCTGCAACT
GATGGATGGATTGCTACCCT

Product: putative RNA methylase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MTEHFFAPCPRGLEAVLAAELECLEATSIEILPGGVAFRGSWLTCYRANLESRIASRILWQVARNQYRSEADIYDITHRL
SWPDWFEPRLTIKVNLAAIKCPLRSLDFLTLRIKDAICDKFRTIRGKRPDIDTTTPDIRIHGFLDARQFTLYLDTSGEAL
FKRGLRQTQGDAPLRENLAAGILALAGWHPGIPLLDPMCGSGTLLLEAAQIACQIAPGARRQFAFENLKLFDANSWKKLK
QTAANRQRERIFQSIYGSDLYGNALVNARNNLATAGLAECVALKQANVLEISPPAETGMLISNPPYGVRVGDHQTLAEFY
PQLGNVLKQRFSGWQAFLLTADPLLAKSIRLTPSRRTPLFNGALECRLLEYRLVAGSMRREKPPNESSKNSHKL

Sequences:

>Translated_394_residues
MTEHFFAPCPRGLEAVLAAELECLEATSIEILPGGVAFRGSWLTCYRANLESRIASRILWQVARNQYRSEADIYDITHRL
SWPDWFEPRLTIKVNLAAIKCPLRSLDFLTLRIKDAICDKFRTIRGKRPDIDTTTPDIRIHGFLDARQFTLYLDTSGEAL
FKRGLRQTQGDAPLRENLAAGILALAGWHPGIPLLDPMCGSGTLLLEAAQIACQIAPGARRQFAFENLKLFDANSWKKLK
QTAANRQRERIFQSIYGSDLYGNALVNARNNLATAGLAECVALKQANVLEISPPAETGMLISNPPYGVRVGDHQTLAEFY
PQLGNVLKQRFSGWQAFLLTADPLLAKSIRLTPSRRTPLFNGALECRLLEYRLVAGSMRREKPPNESSKNSHKL
>Mature_393_residues
TEHFFAPCPRGLEAVLAAELECLEATSIEILPGGVAFRGSWLTCYRANLESRIASRILWQVARNQYRSEADIYDITHRLS
WPDWFEPRLTIKVNLAAIKCPLRSLDFLTLRIKDAICDKFRTIRGKRPDIDTTTPDIRIHGFLDARQFTLYLDTSGEALF
KRGLRQTQGDAPLRENLAAGILALAGWHPGIPLLDPMCGSGTLLLEAAQIACQIAPGARRQFAFENLKLFDANSWKKLKQ
TAANRQRERIFQSIYGSDLYGNALVNARNNLATAGLAECVALKQANVLEISPPAETGMLISNPPYGVRVGDHQTLAEFYP
QLGNVLKQRFSGWQAFLLTADPLLAKSIRLTPSRRTPLFNGALECRLLEYRLVAGSMRREKPPNESSKNSHKL

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=397, Percent_Identity=34.7607052896725, Blast_Score=229, Evalue=2e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 44075; Mature: 43944

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE ; PS01261 UPF0020

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEHFFAPCPRGLEAVLAAELECLEATSIEILPGGVAFRGSWLTCYRANLESRIASRILW
CCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCEEEECCEEEEEHHCHHHHHHHHHHH
QVARNQYRSEADIYDITHRLSWPDWFEPRLTIKVNLAAIKCPLRSLDFLTLRIKDAICDK
HHHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEEEEEEEEECCCCCCCEEEEEHHHHHHHH
FRTIRGKRPDIDTTTPDIRIHGFLDARQFTLYLDTSGEALFKRGLRQTQGDAPLRENLAA
HHHHCCCCCCCCCCCCCEEEEEEEECEEEEEEEECCCHHHHHHHHHHCCCCCCHHHHHHH
GILALAGWHPGIPLLDPMCGSGTLLLEAAQIACQIAPGARRQFAFENLKLFDANSWKKLK
HHHHHHCCCCCCCCCCCCCCCCCEEEEEHHHHEECCCCCHHHHHHHCEEEECCCHHHHHH
QTAANRQRERIFQSIYGSDLYGNALVNARNNLATAGLAECVALKQANVLEISPPAETGML
HHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHCCCCEEEECCCCCCCEE
ISNPPYGVRVGDHQTLAEFYPQLGNVLKQRFSGWQAFLLTADPLLAKSIRLTPSRRTPLF
ECCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHEECCCCCCCCCC
NGALECRLLEYRLVAGSMRREKPPNESSKNSHKL
CCCHHEEEEHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TEHFFAPCPRGLEAVLAAELECLEATSIEILPGGVAFRGSWLTCYRANLESRIASRILW
CCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCEEEECCEEEEEHHCHHHHHHHHHHH
QVARNQYRSEADIYDITHRLSWPDWFEPRLTIKVNLAAIKCPLRSLDFLTLRIKDAICDK
HHHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEEEEEEEEECCCCCCCEEEEEHHHHHHHH
FRTIRGKRPDIDTTTPDIRIHGFLDARQFTLYLDTSGEALFKRGLRQTQGDAPLRENLAA
HHHHCCCCCCCCCCCCCEEEEEEEECEEEEEEEECCCHHHHHHHHHHCCCCCCHHHHHHH
GILALAGWHPGIPLLDPMCGSGTLLLEAAQIACQIAPGARRQFAFENLKLFDANSWKKLK
HHHHHHCCCCCCCCCCCCCCCCCEEEEEHHHHEECCCCCHHHHHHHCEEEECCCHHHHHH
QTAANRQRERIFQSIYGSDLYGNALVNARNNLATAGLAECVALKQANVLEISPPAETGML
HHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHCCCCEEEECCCCCCCEE
ISNPPYGVRVGDHQTLAEFYPQLGNVLKQRFSGWQAFLLTADPLLAKSIRLTPSRRTPLF
ECCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHEECCCCCCCCCC
NGALECRLLEYRLVAGSMRREKPPNESSKNSHKL
CCCHHEEEEHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA