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The map label for this gene is purB [H]

Identifier: 113477587

GI number: 113477587

Start: 6431811

End: 6433106

Strand: Reverse

Name: purB [H]

Synonym: Tery_4169

Alternate gene names: 113477587

Gene position: 6433106-6431811 (Counterclockwise)

Preceding gene: 113477588

Following gene: 113477586

Centisome position: 83.01

GC content: 39.51

Gene sequence:

>1296_bases
GTGATAGAGCGTTATACTTTACCCGAAATGGGTGAACTTTGGACTGATAATACCAAGCTAAAAACTTGGCTACAAGTGGA
AATAGCTGTCTGTGAAGCTCAGGCAGAACTTGGTTATATACCTGCTGAGGCCGTAGCAGAAATTAAGGCCAAGGCTAATT
TTGACCTAAAGCGGGTATTGGAAATTGAAGAGGAAGTCCGCCATGATATGATTGCCTTTTTAACAAATGTTAATGAATAT
GTTGGTGATGTCGGACGATATATTCACCTGGGTTTAACAAGTTCTGATGTTTTGGATACTGCCCTGGCATTACAAATGAT
AGGGAGTCTTGATCTGTTGTTGTCGTGTATAGAAGAATTAGGACAAGCAATTCGCTATCAAGCACAACAACATCGGAAAA
CTGTCATGGTTGGGCGCTCCCATGGTATTCATGCAGAACCAATGACGTTTGGTTTTAAATTAGCAGGATGGTTGGCAGAG
GTTTGTCGTCATCGTCAGAGGTTAGTACAACTACGTCAGAATATTGCTGTGGGGAAAATTTCTGGGGCAGTGGGAACTTA
TGCAAATATTGATCCACGAGTTGAGGCGATCGCTTGTCAAAAATTGGAACTTGAACCGGATACAGCTTCTACTCAGGTAA
TTTCTCGCGATCGTCATGCTGAGTATATACAAACCCTCGCCTTGCTAACTGCTTCTATTGAGCGTTTTGCGGTGGAAATT
CGAAACCTCCAACGCACAGATGTATTGGAGGTTGAGGAATATTTTTCTCAAGGTCAAAAAGGTTCTAGTGCCATGCCTCA
CAAACGTAACCCTATCCGTTCGGAAAGGTTAACAGGAATGGCAAGAATTGTGCGCAGTTATGCTGTTGCTGCTTTGGAAA
ATGTTGCCCTTTGGCACGAACGAGATATTTCTCATAGTTCAGTAGAAAGAGTTATTTTACCAGATGCTTGTATTTTGACT
CATTTTATGCTTAAAGAAACTACTGAGTTAGTCAAGAAATTGTTAGTGTACCCAGAAAACATGAAACGAAATATGAATGT
TTATGGAGGGGTAATTTTTAGCCAACGAGTGCTTTTAGCTTTAGTAGAAAAAGGCATGATCCGAGAAGATGCTTATAAAA
TTGTTCAGTCTTGTGCTCATAAAGCATGGAATCAACCAAATGGTAATTTTCAAGATTTAATTAGTCAAAATGAACAGATT
AAAAGTCACTTATCTGAGGTAGAAATTACAGCTTGTTTTGACGCAAAACACCACTTAAAACATTTAGATGATGTCTATCA
AAGATTAGGTATTTGA

Upstream 100 bases:

>100_bases
AACCTAACACTTAATACCTTGTAATAATCATTTTTTAAAATGATATAATACCTAGTCCTCAGTAGTACAATGTAACGGTT
GTTTGAGTTACAGATAGAGT

Downstream 100 bases:

>100_bases
AGTAATATGTTTGACCATATTTCCTAAGCATTTAGCAGCCAGCTATCAGCTATCAGCTTCTGAGACATACAGCGCTTTCT
GTTGTTATAAGGTACAGGTA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MIERYTLPEMGELWTDNTKLKTWLQVEIAVCEAQAELGYIPAEAVAEIKAKANFDLKRVLEIEEEVRHDMIAFLTNVNEY
VGDVGRYIHLGLTSSDVLDTALALQMIGSLDLLLSCIEELGQAIRYQAQQHRKTVMVGRSHGIHAEPMTFGFKLAGWLAE
VCRHRQRLVQLRQNIAVGKISGAVGTYANIDPRVEAIACQKLELEPDTASTQVISRDRHAEYIQTLALLTASIERFAVEI
RNLQRTDVLEVEEYFSQGQKGSSAMPHKRNPIRSERLTGMARIVRSYAVAALENVALWHERDISHSSVERVILPDACILT
HFMLKETTELVKKLLVYPENMKRNMNVYGGVIFSQRVLLALVEKGMIREDAYKIVQSCAHKAWNQPNGNFQDLISQNEQI
KSHLSEVEITACFDAKHHLKHLDDVYQRLGI

Sequences:

>Translated_431_residues
MIERYTLPEMGELWTDNTKLKTWLQVEIAVCEAQAELGYIPAEAVAEIKAKANFDLKRVLEIEEEVRHDMIAFLTNVNEY
VGDVGRYIHLGLTSSDVLDTALALQMIGSLDLLLSCIEELGQAIRYQAQQHRKTVMVGRSHGIHAEPMTFGFKLAGWLAE
VCRHRQRLVQLRQNIAVGKISGAVGTYANIDPRVEAIACQKLELEPDTASTQVISRDRHAEYIQTLALLTASIERFAVEI
RNLQRTDVLEVEEYFSQGQKGSSAMPHKRNPIRSERLTGMARIVRSYAVAALENVALWHERDISHSSVERVILPDACILT
HFMLKETTELVKKLLVYPENMKRNMNVYGGVIFSQRVLLALVEKGMIREDAYKIVQSCAHKAWNQPNGNFQDLISQNEQI
KSHLSEVEITACFDAKHHLKHLDDVYQRLGI
>Mature_431_residues
MIERYTLPEMGELWTDNTKLKTWLQVEIAVCEAQAELGYIPAEAVAEIKAKANFDLKRVLEIEEEVRHDMIAFLTNVNEY
VGDVGRYIHLGLTSSDVLDTALALQMIGSLDLLLSCIEELGQAIRYQAQQHRKTVMVGRSHGIHAEPMTFGFKLAGWLAE
VCRHRQRLVQLRQNIAVGKISGAVGTYANIDPRVEAIACQKLELEPDTASTQVISRDRHAEYIQTLALLTASIERFAVEI
RNLQRTDVLEVEEYFSQGQKGSSAMPHKRNPIRSERLTGMARIVRSYAVAALENVALWHERDISHSSVERVILPDACILT
HFMLKETTELVKKLLVYPENMKRNMNVYGGVIFSQRVLLALVEKGMIREDAYKIVQSCAHKAWNQPNGNFQDLISQNEQI
KSHLSEVEITACFDAKHHLKHLDDVYQRLGI

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=443, Percent_Identity=27.765237020316, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI183227688, Length=385, Percent_Identity=28.5714285714286, Blast_Score=151, Evalue=1e-36,
Organism=Escherichia coli, GI1787376, Length=318, Percent_Identity=30.8176100628931, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17508577, Length=383, Percent_Identity=26.8929503916449, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI32564234, Length=347, Percent_Identity=28.2420749279539, Blast_Score=109, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6323391, Length=396, Percent_Identity=25.2525252525253, Blast_Score=133, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24647570, Length=410, Percent_Identity=25.8536585365854, Blast_Score=123, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48869; Mature: 48869

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIERYTLPEMGELWTDNTKLKTWLQVEIAVCEAQAELGYIPAEAVAEIKAKANFDLKRVL
CCCCCCCCCHHHHHCCCCHHHHHHEEHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHH
EIEEEVRHDMIAFLTNVNEYVGDVGRYIHLGLTSSDVLDTALALQMIGSLDLLLSCIEEL
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GQAIRYQAQQHRKTVMVGRSHGIHAEPMTFGFKLAGWLAEVCRHRQRLVQLRQNIAVGKI
HHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
SGAVGTYANIDPRVEAIACQKLELEPDTASTQVISRDRHAEYIQTLALLTASIERFAVEI
CCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLQRTDVLEVEEYFSQGQKGSSAMPHKRNPIRSERLTGMARIVRSYAVAALENVALWHE
HHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDISHSSVERVILPDACILTHFMLKETTELVKKLLVYPENMKRNMNVYGGVIFSQRVLLA
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHH
LVEKGMIREDAYKIVQSCAHKAWNQPNGNFQDLISQNEQIKSHLSEVEITACFDAKHHLK
HHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHH
HLDDVYQRLGI
HHHHHHHHHCC
>Mature Secondary Structure
MIERYTLPEMGELWTDNTKLKTWLQVEIAVCEAQAELGYIPAEAVAEIKAKANFDLKRVL
CCCCCCCCCHHHHHCCCCHHHHHHEEHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHH
EIEEEVRHDMIAFLTNVNEYVGDVGRYIHLGLTSSDVLDTALALQMIGSLDLLLSCIEEL
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GQAIRYQAQQHRKTVMVGRSHGIHAEPMTFGFKLAGWLAEVCRHRQRLVQLRQNIAVGKI
HHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
SGAVGTYANIDPRVEAIACQKLELEPDTASTQVISRDRHAEYIQTLALLTASIERFAVEI
CCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLQRTDVLEVEEYFSQGQKGSSAMPHKRNPIRSERLTGMARIVRSYAVAALENVALWHE
HHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDISHSSVERVILPDACILTHFMLKETTELVKKLLVYPENMKRNMNVYGGVIFSQRVLLA
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHH
LVEKGMIREDAYKIVQSCAHKAWNQPNGNFQDLISQNEQIKSHLSEVEITACFDAKHHLK
HHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHH
HLDDVYQRLGI
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]