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The map label for this gene is rpoD3 [H]

Identifier: 113477482

GI number: 113477482

Start: 6266294

End: 6267253

Strand: Reverse

Name: rpoD3 [H]

Synonym: Tery_4058

Alternate gene names: 113477482

Gene position: 6267253-6266294 (Counterclockwise)

Preceding gene: 113477483

Following gene: 113477481

Centisome position: 80.87

GC content: 39.69

Gene sequence:

>960_bases
ATGAAGATTGGTAAAACCTCAGACATAGATTCTGTATGCACTTATCTAAGAGAAATTGGTCGCGTTCCTCTATTAAGCCA
CGAAGAAGAAATTCTTTATGGAAAACAAGTTCAACGCTTAACGGCTTTGCAGGAATTTAACCAAACGTTGGCAGAAGCTT
TAGGCCGTGAACCAACATTATTAGAATGGGCTGAGGCCAAGGAGCTGTCGAAGTCTGAATTGCAGCGAATTATTAGGGAA
GGCGAAAGAGCCAAGCGAAAAATGGTAGAGGCAAATTTGCGTTTGGTAGTATCTGTTGCTAAAAAATATATCAAGCGAAA
TGTAGATTTGCTTGATTTAATTCAAGAAGGCACCATTGGTATGCAGCGAGGTGTAGAAAAGTTTGACCCGACTAAGGGCT
ATAGATTTTCTACTTATGCTTATTGGTGGATTCGTCAGGCTATAACCAGAGCAATAGCAGAAAAAGGCCGCACAATTCGT
TTACCTATCCATATTACAGAAAAGCTAAATAAGATTAAAAAAGTTCAGCGCCAACTGACTCAACAGCTAGGACGTTCGGC
TACTACTGGTGAAATAGCAGAAGAACTAGGTTTAACTCCAAAACAAATACGGGAATGCTTAGAACGGGCTCGCTTACCTT
TATCTCTAGATTTGCGAGTGGGAGATAATCAAGATACAGAATTAGGAGACTTACTAGAAGATACAGGAGCTTCTCCAGAA
GATTATGCTCTCCAATCTTCCATGCGAACTGATTTAGAATCAATCATGGTTGACCTTACTCCTCAACAAAAGCAAGTTTT
GGCCTTAAGGTTTGGTTTAGAAGATGGCCAAACTATGACTCTATCGAAAATTGGTGCCCACCTGAATATTAGTCGTGAAA
GAGTACGGCAGATAGAGCGGGAAGCTTTAAGTAAACTTCGTAAAAGAAAACAAGATATGAATGATTATTTAGCTAGTTAG

Upstream 100 bases:

>100_bases
CAGAGAAAAAAACTTTTTTTAAGAAAAATTCAAGAAAAGCGTAATGCCCGCCCGCTTTTCTTACTATCTCAAAGTAGATT
TATCTAGCGGGATGAAGATT

Downstream 100 bases:

>100_bases
GGCAAATACATAGACGTTTGTTTAGTATTCTTAGGGCTATGTACTGTAGAGAAACCCTAAGACTATACTGGAAGTAACCT
CAGATTTATTTCCGATGAAA

Product: RNA polymerase sigma factor SigD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKIGKTSDIDSVCTYLREIGRVPLLSHEEEILYGKQVQRLTALQEFNQTLAEALGREPTLLEWAEAKELSKSELQRIIRE
GERAKRKMVEANLRLVVSVAKKYIKRNVDLLDLIQEGTIGMQRGVEKFDPTKGYRFSTYAYWWIRQAITRAIAEKGRTIR
LPIHITEKLNKIKKVQRQLTQQLGRSATTGEIAEELGLTPKQIRECLERARLPLSLDLRVGDNQDTELGDLLEDTGASPE
DYALQSSMRTDLESIMVDLTPQQKQVLALRFGLEDGQTMTLSKIGAHLNISRERVRQIEREALSKLRKRKQDMNDYLAS

Sequences:

>Translated_319_residues
MKIGKTSDIDSVCTYLREIGRVPLLSHEEEILYGKQVQRLTALQEFNQTLAEALGREPTLLEWAEAKELSKSELQRIIRE
GERAKRKMVEANLRLVVSVAKKYIKRNVDLLDLIQEGTIGMQRGVEKFDPTKGYRFSTYAYWWIRQAITRAIAEKGRTIR
LPIHITEKLNKIKKVQRQLTQQLGRSATTGEIAEELGLTPKQIRECLERARLPLSLDLRVGDNQDTELGDLLEDTGASPE
DYALQSSMRTDLESIMVDLTPQQKQVLALRFGLEDGQTMTLSKIGAHLNISRERVRQIEREALSKLRKRKQDMNDYLAS
>Mature_319_residues
MKIGKTSDIDSVCTYLREIGRVPLLSHEEEILYGKQVQRLTALQEFNQTLAEALGREPTLLEWAEAKELSKSELQRIIRE
GERAKRKMVEANLRLVVSVAKKYIKRNVDLLDLIQEGTIGMQRGVEKFDPTKGYRFSTYAYWWIRQAITRAIAEKGRTIR
LPIHITEKLNKIKKVQRQLTQQLGRSATTGEIAEELGLTPKQIRECLERARLPLSLDLRVGDNQDTELGDLLEDTGASPE
DYALQSSMRTDLESIMVDLTPQQKQVLALRFGLEDGQTMTLSKIGAHLNISRERVRQIEREALSKLRKRKQDMNDYLAS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=226, Percent_Identity=47.787610619469, Blast_Score=234, Evalue=6e-63,
Organism=Escherichia coli, GI1789098, Length=301, Percent_Identity=36.8770764119601, Blast_Score=191, Evalue=7e-50,
Organism=Escherichia coli, GI1789871, Length=241, Percent_Identity=27.8008298755187, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1788231, Length=208, Percent_Identity=29.8076923076923, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 36562; Mature: 36562

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGKTSDIDSVCTYLREIGRVPLLSHEEEILYGKQVQRLTALQEFNQTLAEALGREPTL
CCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
LEWAEAKELSKSELQRIIREGERAKRKMVEANLRLVVSVAKKYIKRNVDLLDLIQEGTIG
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCC
MQRGVEKFDPTKGYRFSTYAYWWIRQAITRAIAEKGRTIRLPIHITEKLNKIKKVQRQLT
HHHCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHH
QQLGRSATTGEIAEELGLTPKQIRECLERARLPLSLDLRVGDNQDTELGDLLEDTGASPE
HHHCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHCCCCCH
DYALQSSMRTDLESIMVDLTPQQKQVLALRFGLEDGQTMTLSKIGAHLNISRERVRQIER
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEHHHHHCCCCCCCHHHHHHHHH
EALSKLRKRKQDMNDYLAS
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIGKTSDIDSVCTYLREIGRVPLLSHEEEILYGKQVQRLTALQEFNQTLAEALGREPTL
CCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
LEWAEAKELSKSELQRIIREGERAKRKMVEANLRLVVSVAKKYIKRNVDLLDLIQEGTIG
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCC
MQRGVEKFDPTKGYRFSTYAYWWIRQAITRAIAEKGRTIRLPIHITEKLNKIKKVQRQLT
HHHCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHH
QQLGRSATTGEIAEELGLTPKQIRECLERARLPLSLDLRVGDNQDTELGDLLEDTGASPE
HHHCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHCCCCCH
DYALQSSMRTDLESIMVDLTPQQKQVLALRFGLEDGQTMTLSKIGAHLNISRERVRQIER
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEHHHHHCCCCCCCHHHHHHHHH
EALSKLRKRKQDMNDYLAS
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA