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The map label for this gene is yfkE [H]

Identifier: 113477108

GI number: 113477108

Start: 5574671

End: 5575789

Strand: Reverse

Name: yfkE [H]

Synonym: Tery_3626

Alternate gene names: 113477108

Gene position: 5575789-5574671 (Counterclockwise)

Preceding gene: 113477109

Following gene: 113477107

Centisome position: 71.94

GC content: 33.78

Gene sequence:

>1119_bases
ATGCTAAACGCACAAACAATAATTTCAATTTTATTAGTATTTGTTCCTATTTCAATAGCTGCTAATGCTATGGAATTGGG
TTCATCAGTAATTTTTATTACATCTGCTATAGCAATTATTCCTTTGGCAGGTTGGATGGGTACAGCAACGGAAGAAATTG
CAGTCGTTCTAGGTCCTAACTTAGGTGGTTTATTAAATGCTACATTTGGTAATGCTACAGAATTAATTATCGGTATTATT
GCTTTAAATGCTGGTTTAGTAAGGGTAGTTAAAGCAAGCATTACCGGTTCTATAATTGGTAACTTATTGTTAGTAATGGG
CCTCTCAATGTTTTTAGGTGGACTCCGTTTCAAAGAACAAAAATTTCAGTCAGTAGTAGCTCGCTTAAATGCTTCAGCAA
TGAATTTAGCTGTAATTGCTATTCTAGTACCAACAGCAGTGAATGCTACTTCCAATGGTATTAGTAGTAATACAATGCAA
AATTTGTCTGTCGCAGTAGGAATAGTATTAATTACTGTTTATATACTTACCTTACTATTTTCCATGAAAACTCACTCTTA
CTTATATGAAGTTGGTATAGTAGGAAGTGAATCACAGGATTTAGATTCTGATAGTGAATCAGAAGAAAATGCCGAACATA
AACCTAATTTGTTACTGTGGATTTTAGTGTTATTAATAGCAACTTTAACAGTAGCATTTGAATCAGAATTATTGGTTGAT
TCTTTAGAAGAAGCTACTTCTGCATTAGGATTAACTGCTTTATTTACTGGTGTAATTGTAGTACCAATTATTGGTAATGC
TGCCGAACACGCTACAGCAGTAACTGTTGCCATGAAAAATAAAATGGATTTATCTGTAAGTGTAGCAGTTGGCTCTAGTT
TACAAATTGCGTTATTTGTGGCACCTGTATTAATTCTGACTGGTTCATTGCTAGGTCAACCAATGGATTTGAATTTTAAT
CCTTTTGAATTAGTAGCAGTAGTAGTAGCAGTATTAATTGCTAATTCTGTCAGTTCTGATGGTCGTTCTGACTGGTTAGA
AGGGGTTTTATTATTAGCAGCTTATACTATTTTAGGACTGGCCTTTTATTTTCACCCTGTAATTAAAGGATTGAGTTAG

Upstream 100 bases:

>100_bases
TCGGGAATAAATTGCAGATAGGCAAATGACCTGAAAACTTATCAATTATGTCTGTTAATTTTGGCGTAACTATGATTTAC
CAAACCCCCAATTTACACTT

Downstream 100 bases:

>100_bases
ACTTTATAATTAATTATACTTTAGCAACACATAAGTACAAAATAGTATCAAACCATTACATAGCAATTGTTTGATATTCT
CATCTACTTGTTTCCATTTT

Product: calcium/cation antiporter

Products: Na (I) [Periplasm]; Ca (II) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MLNAQTIISILLVFVPISIAANAMELGSSVIFITSAIAIIPLAGWMGTATEEIAVVLGPNLGGLLNATFGNATELIIGII
ALNAGLVRVVKASITGSIIGNLLLVMGLSMFLGGLRFKEQKFQSVVARLNASAMNLAVIAILVPTAVNATSNGISSNTMQ
NLSVAVGIVLITVYILTLLFSMKTHSYLYEVGIVGSESQDLDSDSESEENAEHKPNLLLWILVLLIATLTVAFESELLVD
SLEEATSALGLTALFTGVIVVPIIGNAAEHATAVTVAMKNKMDLSVSVAVGSSLQIALFVAPVLILTGSLLGQPMDLNFN
PFELVAVVVAVLIANSVSSDGRSDWLEGVLLLAAYTILGLAFYFHPVIKGLS

Sequences:

>Translated_372_residues
MLNAQTIISILLVFVPISIAANAMELGSSVIFITSAIAIIPLAGWMGTATEEIAVVLGPNLGGLLNATFGNATELIIGII
ALNAGLVRVVKASITGSIIGNLLLVMGLSMFLGGLRFKEQKFQSVVARLNASAMNLAVIAILVPTAVNATSNGISSNTMQ
NLSVAVGIVLITVYILTLLFSMKTHSYLYEVGIVGSESQDLDSDSESEENAEHKPNLLLWILVLLIATLTVAFESELLVD
SLEEATSALGLTALFTGVIVVPIIGNAAEHATAVTVAMKNKMDLSVSVAVGSSLQIALFVAPVLILTGSLLGQPMDLNFN
PFELVAVVVAVLIANSVSSDGRSDWLEGVLLLAAYTILGLAFYFHPVIKGLS
>Mature_372_residues
MLNAQTIISILLVFVPISIAANAMELGSSVIFITSAIAIIPLAGWMGTATEEIAVVLGPNLGGLLNATFGNATELIIGII
ALNAGLVRVVKASITGSIIGNLLLVMGLSMFLGGLRFKEQKFQSVVARLNASAMNLAVIAILVPTAVNATSNGISSNTMQ
NLSVAVGIVLITVYILTLLFSMKTHSYLYEVGIVGSESQDLDSDSESEENAEHKPNLLLWILVLLIATLTVAFESELLVD
SLEEATSALGLTALFTGVIVVPIIGNAAEHATAVTVAMKNKMDLSVSVAVGSSLQIALFVAPVLILTGSLLGQPMDLNFN
PFELVAVVVAVLIANSVSSDGRSDWLEGVLLLAAYTILGLAFYFHPVIKGLS

Specific function: According To Ref.1 The Natural Activity Of This Protein Is As A Calcium/Proton Antiporter, But It Can Act As A Weak Sodium/Proton Antiporter. The Presence Of A Preferred Substrate And Normal Ph Cytoplasmic Values Might Prevent Significant Sodium/Proton An

COG id: COG0387

COG function: function code P; Ca2+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium/potassium/calcium exchanger family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6320075, Length=373, Percent_Identity=40.7506702412869, Blast_Score=253, Evalue=3e-68,
Organism=Saccharomyces cerevisiae, GI6324008, Length=380, Percent_Identity=25.2631578947368, Blast_Score=117, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004713
- InterPro:   IPR004798
- InterPro:   IPR004837 [H]

Pfam domain/function: PF01699 Na_Ca_ex [H]

EC number: NA

Molecular weight: Translated: 38846; Mature: 38846

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNAQTIISILLVFVPISIAANAMELGSSVIFITSAIAIIPLAGWMGTATEEIAVVLGPN
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHEEEEECCC
LGGLLNATFGNATELIIGIIALNAGLVRVVKASITGSIIGNLLLVMGLSMFLGGLRFKEQ
CCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
KFQSVVARLNASAMNLAVIAILVPTAVNATSNGISSNTMQNLSVAVGIVLITVYILTLLF
HHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SMKTHSYLYEVGIVGSESQDLDSDSESEENAEHKPNLLLWILVLLIATLTVAFESELLVD
HHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SLEEATSALGLTALFTGVIVVPIIGNAAEHATAVTVAMKNKMDLSVSVAVGSSLQIALFV
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEEEEECCCCCEEEEEEECCCHHHHHHH
APVLILTGSLLGQPMDLNFNPFELVAVVVAVLIANSVSSDGRSDWLEGVLLLAAYTILGL
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
AFYFHPVIKGLS
HHHHHHHHHCCC
>Mature Secondary Structure
MLNAQTIISILLVFVPISIAANAMELGSSVIFITSAIAIIPLAGWMGTATEEIAVVLGPN
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHEEEEECCC
LGGLLNATFGNATELIIGIIALNAGLVRVVKASITGSIIGNLLLVMGLSMFLGGLRFKEQ
CCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
KFQSVVARLNASAMNLAVIAILVPTAVNATSNGISSNTMQNLSVAVGIVLITVYILTLLF
HHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SMKTHSYLYEVGIVGSESQDLDSDSESEENAEHKPNLLLWILVLLIATLTVAFESELLVD
HHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SLEEATSALGLTALFTGVIVVPIIGNAAEHATAVTVAMKNKMDLSVSVAVGSSLQIALFV
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEEEEECCCCCEEEEEEECCCHHHHHHH
APVLILTGSLLGQPMDLNFNPFELVAVVVAVLIANSVSSDGRSDWLEGVLLLAAYTILGL
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
AFYFHPVIKGLS
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Cytoplasm]; Proton [Periplasm]; Ca (II) [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: Na (I) [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + Na (I) [Periplasm] Ca (II) [Cytoplasm] + Proton [Periplasm] = Ca (II) [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]