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The map label for this gene is bioB

Identifier: 113477033

GI number: 113477033

Start: 5445853

End: 5446965

Strand: Reverse

Name: bioB

Synonym: Tery_3533

Alternate gene names: 113477033

Gene position: 5446965-5445853 (Counterclockwise)

Preceding gene: 113477035

Following gene: 113477032

Centisome position: 70.28

GC content: 40.7

Gene sequence:

>1113_bases
ATGATTGCCGTGCTCAAAACACCTTTATCAAATCATATAAAAGCAAATACTAAAGCTTTATTCTCATCTTTGCCAAAGAC
AAAAAATGAGTTGGAGATATCCCTTGATGATTTGGCTAAACAAATTATTTCTGGGTATCGCCTCAGTCGAGAAGAGGCAA
TTATACTGACTCAAATAGCAGGAGAAGAGGATATTCTTCTTCTGTGTGAAGCTGCTGATCGGGTTCGTCAAGCTTGCTGT
GGAAATGTTGTAGACCTGTGTAGCATTATTAATGTTAAGTCTGGAGGCTGTTCAGAAAATTGTAGTTTCTGTTCCCAGTC
AGTTCATCATCCTGGAGAAGATTCTCCTATTTATGGCCTTAAGTCTACTGAAGAAATCTTAGATCAAGCTAAGGCAGCAG
AAGCTGCTGGAGCTAAAAGATTTTGCTTGGTGTCTCAGGGTCGGGGTGTTAAGTATAATAGTCCTAAGTCTACAGAATTT
GAACAAATTCTGGGGACAGTAAGACAAATTCTGGCTGAAACTAATATTAAGCCTTGTTGTGCTTTGGGAGAGCTGACCCC
AGAACAAGCTCAAGCATTGGCCGAAGCAGGAGTGACTCGCTACAATCATAATTTGGAAGCTTCTGAGCATTTTTACCCGG
AAATTGTAAGTACTCATAGCTGGAATGACCGAGTTGAAACTGTGAAAAACCTAAAGGCAGCAGGTATTCAAGCTTGCACA
GGAGGTATTATTGGTATGGGTGAAACTTGGGCAGATAGGATAGATTTGGCCTTGGCCTTGCGAGAGTTAGAGGTTGAGTC
TGTACCTTTGAATTTGTTAAATTCTCGCGAAGGTACTCCACTAGGTGGTTTGCTTAAGTTAGATCCTTATGATGCTTTGA
AAGCGATCGCTATTTTTCGTCTAATTTTACCAAAGCAAATTATTCGCTATGCTGGAGGGCGAGAAGCTGTAATGGGAGAT
TTGCAAGCTTTGGGTTTGAAGGCTGGTATTAATGCTATGTTAATTGGCCATTATCTGACAACTCTTGGTCAACCTCCAGA
AAAAGATCAAGCAATGCTAAAATCTCTTGGACTTCAAGGTGGTGAAACTCCTATTGCAACTAATGGCTTGTAA

Upstream 100 bases:

>100_bases
AACTGAGCAGCCAAAGCTCAAAATCATGTTTTGAGTAAGTTTAGTAAAACAGATAAAAATGGTAGAATTTGGACGATATT
AAAAATAACTTCAACAATAA

Downstream 100 bases:

>100_bases
AGATTCAGAGATCAGTTTTAGCTATCAGTTATTGCTTTTGATAATTACTCAAAACTTTATTAATTGATCCAAAATCTGAG
TTTGAGGGTAATCTCTTTTG

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MIAVLKTPLSNHIKANTKALFSSLPKTKNELEISLDDLAKQIISGYRLSREEAIILTQIAGEEDILLLCEAADRVRQACC
GNVVDLCSIINVKSGGCSENCSFCSQSVHHPGEDSPIYGLKSTEEILDQAKAAEAAGAKRFCLVSQGRGVKYNSPKSTEF
EQILGTVRQILAETNIKPCCALGELTPEQAQALAEAGVTRYNHNLEASEHFYPEIVSTHSWNDRVETVKNLKAAGIQACT
GGIIGMGETWADRIDLALALRELEVESVPLNLLNSREGTPLGGLLKLDPYDALKAIAIFRLILPKQIIRYAGGREAVMGD
LQALGLKAGINAMLIGHYLTTLGQPPEKDQAMLKSLGLQGGETPIATNGL

Sequences:

>Translated_370_residues
MIAVLKTPLSNHIKANTKALFSSLPKTKNELEISLDDLAKQIISGYRLSREEAIILTQIAGEEDILLLCEAADRVRQACC
GNVVDLCSIINVKSGGCSENCSFCSQSVHHPGEDSPIYGLKSTEEILDQAKAAEAAGAKRFCLVSQGRGVKYNSPKSTEF
EQILGTVRQILAETNIKPCCALGELTPEQAQALAEAGVTRYNHNLEASEHFYPEIVSTHSWNDRVETVKNLKAAGIQACT
GGIIGMGETWADRIDLALALRELEVESVPLNLLNSREGTPLGGLLKLDPYDALKAIAIFRLILPKQIIRYAGGREAVMGD
LQALGLKAGINAMLIGHYLTTLGQPPEKDQAMLKSLGLQGGETPIATNGL
>Mature_370_residues
MIAVLKTPLSNHIKANTKALFSSLPKTKNELEISLDDLAKQIISGYRLSREEAIILTQIAGEEDILLLCEAADRVRQACC
GNVVDLCSIINVKSGGCSENCSFCSQSVHHPGEDSPIYGLKSTEEILDQAKAAEAAGAKRFCLVSQGRGVKYNSPKSTEF
EQILGTVRQILAETNIKPCCALGELTPEQAQALAEAGVTRYNHNLEASEHFYPEIVSTHSWNDRVETVKNLKAAGIQACT
GGIIGMGETWADRIDLALALRELEVESVPLNLLNSREGTPLGGLLKLDPYDALKAIAIFRLILPKQIIRYAGGREAVMGD
LQALGLKAGINAMLIGHYLTTLGQPPEKDQAMLKSLGLQGGETPIATNGL

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=303, Percent_Identity=35.3135313531353, Blast_Score=189, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6321725, Length=293, Percent_Identity=35.8361774744027, Blast_Score=184, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_TRIEI (Q10YQ3)

Other databases:

- EMBL:   CP000393
- RefSeq:   YP_723094.1
- ProteinModelPortal:   Q10YQ3
- SMR:   Q10YQ3
- STRING:   Q10YQ3
- GeneID:   4244359
- GenomeReviews:   CP000393_GR
- KEGG:   ter:Tery_3533
- NMPDR:   fig|203124.1.peg.1608
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- PhylomeDB:   Q10YQ3
- ProtClustDB:   CLSK892897
- BioCyc:   TERY203124:TERY_3533-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 39818; Mature: 39818

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAVLKTPLSNHIKANTKALFSSLPKTKNELEISLDDLAKQIISGYRLSREEAIILTQIA
CEEEECCCHHHHHHHHHHHHHHHCCCCCCHHEECHHHHHHHHHHHHCCCCCCEEEEEEEC
GEEDILLLCEAADRVRQACCGNVVDLCSIINVKSGGCSENCSFCSQSVHHPGEDSPIYGL
CCCCEEEEEHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCC
KSTEEILDQAKAAEAAGAKRFCLVSQGRGVKYNSPKSTEFEQILGTVRQILAETNIKPCC
CCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHH
ALGELTPEQAQALAEAGVTRYNHNLEASEHFYPEIVSTHSWNDRVETVKNLKAAGIQACT
HHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHCHHHHC
GGIIGMGETWADRIDLALALRELEVESVPLNLLNSREGTPLGGLLKLDPYDALKAIAIFR
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCEEEECHHHHHHHHHHHH
LILPKQIIRYAGGREAVMGDLQALGLKAGINAMLIGHYLTTLGQPPEKDQAMLKSLGLQG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
GETPIATNGL
CCCCCCCCCC
>Mature Secondary Structure
MIAVLKTPLSNHIKANTKALFSSLPKTKNELEISLDDLAKQIISGYRLSREEAIILTQIA
CEEEECCCHHHHHHHHHHHHHHHCCCCCCHHEECHHHHHHHHHHHHCCCCCCEEEEEEEC
GEEDILLLCEAADRVRQACCGNVVDLCSIINVKSGGCSENCSFCSQSVHHPGEDSPIYGL
CCCCEEEEEHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCC
KSTEEILDQAKAAEAAGAKRFCLVSQGRGVKYNSPKSTEFEQILGTVRQILAETNIKPCC
CCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHH
ALGELTPEQAQALAEAGVTRYNHNLEASEHFYPEIVSTHSWNDRVETVKNLKAAGIQACT
HHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHCHHHHC
GGIIGMGETWADRIDLALALRELEVESVPLNLLNSREGTPLGGLLKLDPYDALKAIAIFR
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCEEEECHHHHHHHHHHHH
LILPKQIIRYAGGREAVMGDLQALGLKAGINAMLIGHYLTTLGQPPEKDQAMLKSLGLQG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
GETPIATNGL
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA