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The map label for this gene is truA [H]

Identifier: 113476553

GI number: 113476553

Start: 4642259

End: 4643077

Strand: Reverse

Name: truA [H]

Synonym: Tery_2986

Alternate gene names: 113476553

Gene position: 4643077-4642259 (Counterclockwise)

Preceding gene: 113476554

Following gene: 113476552

Centisome position: 59.91

GC content: 40.66

Gene sequence:

>819_bases
ATGGCTAATAACCAAACTTTAGTTCAGCGCGTTGCCCTTGTGATTCAATATCAAGGTACTCATTTTCATGGGTGGCAAAG
ACAGCCAAATCAACGAACTGTCCAAGAAGAGATTGAGAAGACTATTGCTTCTTTCATTAACGAACCTGTGACGTTACATG
GGGCGGGTAGAACAGATGCTGGAGTTCATGCAGCAGCTCAAGTTGCTCACTTTGATGTTTCAGGTCCTATCCCTGCTCAT
AAATGGGCTACTATTCTAAACTCACGACTGGCTAATGATATATTGATTAGAGGTTCGGCTAGAATAGATTATGCTTGGCA
CTCCCGGTTTACCGCAATTTGGCGACGTTATCGCTATACTATTTATACAGATAAACAACCAAATTTGTTTGTGATGCCTT
TTGTTTGGCATTATTATCAAGCTTCTTTAGACCCACAATTGATTCAAGCAGCTTTGAATCCTTTAATTGGTCATCATCAT
TTGGCTGCTTTTCAAAGAGCTGGATCCTGTCGTCCCCATTCTTGGGTAGATGTACAAGAGGTAATGTGTGAAAGGAAAGG
TCCATTTATCTATATAGAGGTTCAAGCAAGTGGGTTTCTGTATGGAATGATGCGATTATTGGTAGGGTTGCTTATTCAAG
TGGGTACTGAAGAGCTATCTCTGGAAAGTTTTACGGATATTTGGGTTAATCAAAGGAGGGACATTGTGAAGCACTCAGCT
CCAGCTAGTGGTCTTTGTTTATTGCGGGTTGGTTATCCAGAATTTCCTTTTCCTCAAGAAGTTTGGTATGACAATATGCC
TAGATTTATCCTTGGTTAA

Upstream 100 bases:

>100_bases
AAGGCAGTGGGAAGATAGAAAAGGTTAATTTGACCATTTAAAAGTAAGAGCTTAATGTTAATAGATATTGACTTTTAAGC
TTTAGAGACTAACAATTCTC

Downstream 100 bases:

>100_bases
TTTAAATAAGTAGTCAGTACTCTACAATTTAGTCAGATCGATCATTTTTTTATTTGGCAGAAGGGTATTTCAAGATAAAG
ATAATTATAAAAAATTATAC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MANNQTLVQRVALVIQYQGTHFHGWQRQPNQRTVQEEIEKTIASFINEPVTLHGAGRTDAGVHAAAQVAHFDVSGPIPAH
KWATILNSRLANDILIRGSARIDYAWHSRFTAIWRRYRYTIYTDKQPNLFVMPFVWHYYQASLDPQLIQAALNPLIGHHH
LAAFQRAGSCRPHSWVDVQEVMCERKGPFIYIEVQASGFLYGMMRLLVGLLIQVGTEELSLESFTDIWVNQRRDIVKHSA
PASGLCLLRVGYPEFPFPQEVWYDNMPRFILG

Sequences:

>Translated_272_residues
MANNQTLVQRVALVIQYQGTHFHGWQRQPNQRTVQEEIEKTIASFINEPVTLHGAGRTDAGVHAAAQVAHFDVSGPIPAH
KWATILNSRLANDILIRGSARIDYAWHSRFTAIWRRYRYTIYTDKQPNLFVMPFVWHYYQASLDPQLIQAALNPLIGHHH
LAAFQRAGSCRPHSWVDVQEVMCERKGPFIYIEVQASGFLYGMMRLLVGLLIQVGTEELSLESFTDIWVNQRRDIVKHSA
PASGLCLLRVGYPEFPFPQEVWYDNMPRFILG
>Mature_271_residues
ANNQTLVQRVALVIQYQGTHFHGWQRQPNQRTVQEEIEKTIASFINEPVTLHGAGRTDAGVHAAAQVAHFDVSGPIPAHK
WATILNSRLANDILIRGSARIDYAWHSRFTAIWRRYRYTIYTDKQPNLFVMPFVWHYYQASLDPQLIQAALNPLIGHHHL
AAFQRAGSCRPHSWVDVQEVMCERKGPFIYIEVQASGFLYGMMRLLVGLLIQVGTEELSLESFTDIWVNQRRDIVKHSAP
ASGLCLLRVGYPEFPFPQEVWYDNMPRFILG

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=276, Percent_Identity=31.5217391304348, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1788657, Length=251, Percent_Identity=34.6613545816733, Blast_Score=164, Evalue=5e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 31193; Mature: 31062

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANNQTLVQRVALVIQYQGTHFHGWQRQPNQRTVQEEIEKTIASFINEPVTLHGAGRTDA
CCCCHHHHHHHHHHEEECCCEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCC
GVHAAAQVAHFDVSGPIPAHKWATILNSRLANDILIRGSARIDYAWHSRFTAIWRRYRYT
CHHHHHHHEEEECCCCCCHHHHHHHHHHHHHCCEEEECCCEEEHHHHHHHHHHHHHHEEE
IYTDKQPNLFVMPFVWHYYQASLDPQLIQAALNPLIGHHHLAAFQRAGSCRPHSWVDVQE
EEECCCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
VMCERKGPFIYIEVQASGFLYGMMRLLVGLLIQVGTEELSLESFTDIWVNQRRDIVKHSA
HHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC
PASGLCLLRVGYPEFPFPQEVWYDNMPRFILG
CCCCEEEEEECCCCCCCCHHHHHCCCCHHHCC
>Mature Secondary Structure 
ANNQTLVQRVALVIQYQGTHFHGWQRQPNQRTVQEEIEKTIASFINEPVTLHGAGRTDA
CCCHHHHHHHHHHEEECCCEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCC
GVHAAAQVAHFDVSGPIPAHKWATILNSRLANDILIRGSARIDYAWHSRFTAIWRRYRYT
CHHHHHHHEEEECCCCCCHHHHHHHHHHHHHCCEEEECCCEEEHHHHHHHHHHHHHHEEE
IYTDKQPNLFVMPFVWHYYQASLDPQLIQAALNPLIGHHHLAAFQRAGSCRPHSWVDVQE
EEECCCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
VMCERKGPFIYIEVQASGFLYGMMRLLVGLLIQVGTEELSLESFTDIWVNQRRDIVKHSA
HHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC
PASGLCLLRVGYPEFPFPQEVWYDNMPRFILG
CCCCEEEEEECCCCCCCCHHHHHCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]