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The map label for this gene is prfA

Identifier: 113476549

GI number: 113476549

Start: 4637845

End: 4638954

Strand: Reverse

Name: prfA

Synonym: Tery_2982

Alternate gene names: 113476549

Gene position: 4638954-4637845 (Counterclockwise)

Preceding gene: 113476550

Following gene: 113476547

Centisome position: 59.86

GC content: 39.82

Gene sequence:

>1110_bases
ATGGCTGAAACATATTTATTAGATAAGTTAAAATCTGTAGAGCAAACATATAATGAATTAACTCTGCGTTTGGCCGATCC
AGATGTTGCTAAAGACCCCAGTGAGTTCCAAAAATTAGCTAAAGCACGTTCTTCCCTGGAAGAAGTTGTAAATTGTTATG
TAGAGTGGAAAAATGCTCAAGAAGAATTAGCTGATGCTAAGGAAATATTGAAAGAAGCTGTTGGCGATTTGGAAATGCAA
GAGATGGCCAAAGTTGAAGTAGAAGATTTGGAAGCCAAACTAGAATCTCTAGAAAATCAAATGAAAATTGCTCTACTGCC
ACGAGACCCCAACGATGATAAGAATATTATGTTGGAGATTAGAGCTGGTACAGGAGGAGATGAAGCTAGTATTTGGGCAG
GAGATCTCGTTAGAATGTACTCCCGCTACTCAGAGAACCAAAGCTGGAAGGTGAGCTTGCTGAGTGAATCTTTAGCAGAT
ATGGGCGGTTTTAAAGAAGCAATTTTAGAAATTAAAGGGGATCACGTTTATAGTAAGCTCAAATTTGAAGCAGGAGTTCA
TCGGGTTCAAAGAGTGCCAGTTACAGAAGCAGGTGGAAGAGTACATACTTCTACTGCTACAGTGGCGATAATGCCAGAGG
TGGATGATGTGGAAGTTGAAATAGACCAAAAGGATATTGAATTGTCAACTGCTCGTTCTGGTGGAGCTGGTGGACAAAAT
GTCAACAAGGTTGAAACTGCTGTAGATTTGTTTCACAAGCCTACGGGAATTAGAATTTTTTGTACCCAGGAGCGGAGCCA
GCTACAAAACAGGGAGCGAGCAATGCAGATTTTGCGGGCTAAACTTTATGAGATTAAGTTACAAGAGCAACAAGCAGAAG
TGAGTTCTATAAGGCGATCGCAAGTAGGTACTGGTTCCCGTTCTGAAAAAATTCGTACTTATAATTATAAAGATAATCGG
GTGACAGATCATCGCTTAAATCAGAACTTTTCTCTTGTACCTCTTTTGGAGGGAGATATAGAAAATGTTATTCAAGCTTG
TATTACCCAAGATCAACAGGAGCGTTTACAAGAGTTAGCAGCATCTAGTTCTACTCCTATTTCAGTATAG

Upstream 100 bases:

>100_bases
TTTTGCATTTTATCCATCGCTATAATCAATCATTACTTGTTTAGCACTACTGCAAAGGACAACGGACAATGGCTGAAACA
TATTTAAAGGACAACTTATA

Downstream 100 bases:

>100_bases
CTAATATGTTCATTCCCCTTTATGAGAGGAATTGTGGCACTTTTTCTCAAATCTTCTCAGGGTTGTGTTTTGGATTTAGG
AAAAACACCAATAGATCGGC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MAETYLLDKLKSVEQTYNELTLRLADPDVAKDPSEFQKLAKARSSLEEVVNCYVEWKNAQEELADAKEILKEAVGDLEMQ
EMAKVEVEDLEAKLESLENQMKIALLPRDPNDDKNIMLEIRAGTGGDEASIWAGDLVRMYSRYSENQSWKVSLLSESLAD
MGGFKEAILEIKGDHVYSKLKFEAGVHRVQRVPVTEAGGRVHTSTATVAIMPEVDDVEVEIDQKDIELSTARSGGAGGQN
VNKVETAVDLFHKPTGIRIFCTQERSQLQNRERAMQILRAKLYEIKLQEQQAEVSSIRRSQVGTGSRSEKIRTYNYKDNR
VTDHRLNQNFSLVPLLEGDIENVIQACITQDQQERLQELAASSSTPISV

Sequences:

>Translated_369_residues
MAETYLLDKLKSVEQTYNELTLRLADPDVAKDPSEFQKLAKARSSLEEVVNCYVEWKNAQEELADAKEILKEAVGDLEMQ
EMAKVEVEDLEAKLESLENQMKIALLPRDPNDDKNIMLEIRAGTGGDEASIWAGDLVRMYSRYSENQSWKVSLLSESLAD
MGGFKEAILEIKGDHVYSKLKFEAGVHRVQRVPVTEAGGRVHTSTATVAIMPEVDDVEVEIDQKDIELSTARSGGAGGQN
VNKVETAVDLFHKPTGIRIFCTQERSQLQNRERAMQILRAKLYEIKLQEQQAEVSSIRRSQVGTGSRSEKIRTYNYKDNR
VTDHRLNQNFSLVPLLEGDIENVIQACITQDQQERLQELAASSSTPISV
>Mature_368_residues
AETYLLDKLKSVEQTYNELTLRLADPDVAKDPSEFQKLAKARSSLEEVVNCYVEWKNAQEELADAKEILKEAVGDLEMQE
MAKVEVEDLEAKLESLENQMKIALLPRDPNDDKNIMLEIRAGTGGDEASIWAGDLVRMYSRYSENQSWKVSLLSESLADM
GGFKEAILEIKGDHVYSKLKFEAGVHRVQRVPVTEAGGRVHTSTATVAIMPEVDDVEVEIDQKDIELSTARSGGAGGQNV
NKVETAVDLFHKPTGIRIFCTQERSQLQNRERAMQILRAKLYEIKLQEQQAEVSSIRRSQVGTGSRSEKIRTYNYKDNRV
TDHRLNQNFSLVPLLEGDIENVIQACITQDQQERLQELAASSSTPISV

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=281, Percent_Identity=41.6370106761566, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI34577120, Length=254, Percent_Identity=41.7322834645669, Blast_Score=222, Evalue=4e-58,
Organism=Homo sapiens, GI166795305, Length=196, Percent_Identity=37.7551020408163, Blast_Score=152, Evalue=5e-37,
Organism=Escherichia coli, GI1787462, Length=357, Percent_Identity=42.5770308123249, Blast_Score=301, Evalue=3e-83,
Organism=Escherichia coli, GI2367172, Length=334, Percent_Identity=37.125748502994, Blast_Score=208, Evalue=4e-55,
Organism=Caenorhabditis elegans, GI17542784, Length=302, Percent_Identity=36.7549668874172, Blast_Score=194, Evalue=5e-50,
Organism=Saccharomyces cerevisiae, GI6321295, Length=336, Percent_Identity=37.797619047619, Blast_Score=207, Evalue=3e-54,
Organism=Drosophila melanogaster, GI19921226, Length=364, Percent_Identity=34.8901098901099, Blast_Score=209, Evalue=3e-54,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_TRIEI (Q110D7)

Other databases:

- EMBL:   CP000393
- RefSeq:   YP_722610.1
- ProteinModelPortal:   Q110D7
- SMR:   Q110D7
- STRING:   Q110D7
- GeneID:   4245098
- GenomeReviews:   CP000393_GR
- KEGG:   ter:Tery_2982
- NMPDR:   fig|203124.1.peg.5476
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- ProtClustDB:   PRK00591
- BioCyc:   TERY203124:TERY_2982-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 41475; Mature: 41344

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETYLLDKLKSVEQTYNELTLRLADPDVAKDPSEFQKLAKARSSLEEVVNCYVEWKNAQ
CCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHH
EELADAKEILKEAVGDLEMQEMAKVEVEDLEAKLESLENQMKIALLPRDPNDDKNIMLEI
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEEEE
RAGTGGDEASIWAGDLVRMYSRYSENQSWKVSLLSESLADMGGFKEAILEIKGDHVYSKL
EECCCCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCHHHHHHHHCCCHHHHHH
KFEAGVHRVQRVPVTEAGGRVHTSTATVAIMPEVDDVEVEIDQKDIELSTARSGGAGGQN
HHHHHHHHHHCCCCCCCCCEEEECCEEEEEECCCCCCEEEECCCCCEEEECCCCCCCCCC
VNKVETAVDLFHKPTGIRIFCTQERSQLQNRERAMQILRAKLYEIKLQEQQAEVSSIRRS
CHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVGTGSRSEKIRTYNYKDNRVTDHRLNQNFSLVPLLEGDIENVIQACITQDQQERLQELA
HCCCCCCCCCEEEECCCCCCCCHHHCCCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHH
ASSSTPISV
CCCCCCCCC
>Mature Secondary Structure 
AETYLLDKLKSVEQTYNELTLRLADPDVAKDPSEFQKLAKARSSLEEVVNCYVEWKNAQ
CCHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHH
EELADAKEILKEAVGDLEMQEMAKVEVEDLEAKLESLENQMKIALLPRDPNDDKNIMLEI
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEEEE
RAGTGGDEASIWAGDLVRMYSRYSENQSWKVSLLSESLADMGGFKEAILEIKGDHVYSKL
EECCCCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCHHHHHHHHCCCHHHHHH
KFEAGVHRVQRVPVTEAGGRVHTSTATVAIMPEVDDVEVEIDQKDIELSTARSGGAGGQN
HHHHHHHHHHCCCCCCCCCEEEECCEEEEEECCCCCCEEEECCCCCEEEECCCCCCCCCC
VNKVETAVDLFHKPTGIRIFCTQERSQLQNRERAMQILRAKLYEIKLQEQQAEVSSIRRS
CHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVGTGSRSEKIRTYNYKDNRVTDHRLNQNFSLVPLLEGDIENVIQACITQDQQERLQELA
HCCCCCCCCCEEEECCCCCCCCHHHCCCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHH
ASSSTPISV
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA