Click here to switch to the map view.

The map label for this gene is acbC [H]

Identifier: 113476547

GI number: 113476547

Start: 4631340

End: 4632689

Strand: Reverse

Name: acbC [H]

Synonym: Tery_2977

Alternate gene names: 113476547

Gene position: 4632689-4631340 (Counterclockwise)

Preceding gene: 113476549

Following gene: 113476546

Centisome position: 59.78

GC content: 38.59

Gene sequence:

>1350_bases
ATGAGCAACGCAATATCAACCTCTGTACCAGTAATTGAAAAAGTCAACAAGCCAGACTTCGTATTTGATCCAAGTATTCG
GCGCGATCATGCAGAACAATATCGTACTTCAGAGTGGTATGATGGTCGTGGAGAAATAAAGGCAGTGGATGATGGTCGCT
CATTCGAGATTTCTGCAACATTTACCCTCAAATCAGAAGTCAAATTAGTAAATGGAGTTTTAGATCCATCTAACCCTATT
TTAGCTAATATCTATAAAAGTCGTAATCGATGCGTAGCAGTTGTCGATCACATGGTAGATGAACTTTATGGAAAATCATT
GACAAATTATTTTGAGCAGAATGATATTCCTCTGAAAACTCTTGTCTGTCGTGCTTGGGAGTCAGACAAAAATACTGATA
CGGTCCATAAAATATTGCAATTCTTGGGCAAAGATGGTTGTGATGTATCTCGTAACGAACCTGTTTTGATAATTGGAGGT
GGTGTACTCAGTGATGTTGCTGGTTTAGCCTGTAGTTTACAACATCGGCGGACACCTTATGTAATGATTGGAACATCAGT
TGTTGCTGCTATTGATGCTGGACCATCTCCTCGTACCTGCACAAATAGTAAGCTATTTAAAAATGGTATTGGAGCATATC
ATCCCCCTGTGATGACTATAGTAGACCGTACTTTGTTCCGTACTTTGGCAAAAGGTCATATTCGCAACGGAATGGCAGAA
ATTATTAAAATGGCAGTTACTGATGATCAAACATTATTTGAATTGCTGGAAAAATATGGTCGACGTTTAGTTGAAACTCA
TTTTGCTAATTTTGATGCAGATGAGGAACTGGTAAAAATATCTGATGAGGTGATTTATCGTGCTTTGTATTCTTATATGA
AGCATGAAGGAACAAATATGTTTGAAACCTATCAAGACCGACCTCATGCTTACGGTCATACATGGAGTCCCCGTTTTGAG
CCAGTGGTCAAACTAATGCACGGTCATGCTGTAACTATTGGTATGTCTCTTGGAGCTACTTTAGCGTTAGAGATGGGTTG
GATAACAACAAATGAACGTGATCGAATAGTTAACTTGTGTCATTCTATGGGTTTGTCTGTTTACCATGAAATTATCGAAG
ATACAGAGTTAATGCTTCAAGCTCAGAAAAATATAACTCGTAAGCGCGGTCGTAGGTTGTGGGCACCCCTACCTACAGGT
ATTGGTTCTTGTAGTTTTGCGAAGGAAATATCTCCAGATTTATTAACATCTGCTGTTGAGGAACATAAGAAATTTTGTGC
TTCTTTTACTTCTTTTGGTAGTCAAGGAAAAGGTGAAGAAATGTATTTAAGCGACCTAGGTTTGGAGTAA

Upstream 100 bases:

>100_bases
ATTTAAGCTCTTACACTGACTTCAGGTGTAGGCAGACTGGTTTGTTGTGCAATAACATCAGACAATACCTCTAAAGCAAA
CAATTACCTAAATACAAATC

Downstream 100 bases:

>100_bases
AAAAATAGGGAGTCTGCTAATAACCCCTTATGATATGCAATTCTCAAGATAGAGAAATGCGGTTAGCTTAAATGTTTATC
ATTGTCATAGTTGTGCTGCT

Product: 3-dehydroquinate synthase

Products: 3-dehydroquinate; phosphate

Alternate protein names: Sedoheptulose 7-phosphate cyclase [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MSNAISTSVPVIEKVNKPDFVFDPSIRRDHAEQYRTSEWYDGRGEIKAVDDGRSFEISATFTLKSEVKLVNGVLDPSNPI
LANIYKSRNRCVAVVDHMVDELYGKSLTNYFEQNDIPLKTLVCRAWESDKNTDTVHKILQFLGKDGCDVSRNEPVLIIGG
GVLSDVAGLACSLQHRRTPYVMIGTSVVAAIDAGPSPRTCTNSKLFKNGIGAYHPPVMTIVDRTLFRTLAKGHIRNGMAE
IIKMAVTDDQTLFELLEKYGRRLVETHFANFDADEELVKISDEVIYRALYSYMKHEGTNMFETYQDRPHAYGHTWSPRFE
PVVKLMHGHAVTIGMSLGATLALEMGWITTNERDRIVNLCHSMGLSVYHEIIEDTELMLQAQKNITRKRGRRLWAPLPTG
IGSCSFAKEISPDLLTSAVEEHKKFCASFTSFGSQGKGEEMYLSDLGLE

Sequences:

>Translated_449_residues
MSNAISTSVPVIEKVNKPDFVFDPSIRRDHAEQYRTSEWYDGRGEIKAVDDGRSFEISATFTLKSEVKLVNGVLDPSNPI
LANIYKSRNRCVAVVDHMVDELYGKSLTNYFEQNDIPLKTLVCRAWESDKNTDTVHKILQFLGKDGCDVSRNEPVLIIGG
GVLSDVAGLACSLQHRRTPYVMIGTSVVAAIDAGPSPRTCTNSKLFKNGIGAYHPPVMTIVDRTLFRTLAKGHIRNGMAE
IIKMAVTDDQTLFELLEKYGRRLVETHFANFDADEELVKISDEVIYRALYSYMKHEGTNMFETYQDRPHAYGHTWSPRFE
PVVKLMHGHAVTIGMSLGATLALEMGWITTNERDRIVNLCHSMGLSVYHEIIEDTELMLQAQKNITRKRGRRLWAPLPTG
IGSCSFAKEISPDLLTSAVEEHKKFCASFTSFGSQGKGEEMYLSDLGLE
>Mature_448_residues
SNAISTSVPVIEKVNKPDFVFDPSIRRDHAEQYRTSEWYDGRGEIKAVDDGRSFEISATFTLKSEVKLVNGVLDPSNPIL
ANIYKSRNRCVAVVDHMVDELYGKSLTNYFEQNDIPLKTLVCRAWESDKNTDTVHKILQFLGKDGCDVSRNEPVLIIGGG
VLSDVAGLACSLQHRRTPYVMIGTSVVAAIDAGPSPRTCTNSKLFKNGIGAYHPPVMTIVDRTLFRTLAKGHIRNGMAEI
IKMAVTDDQTLFELLEKYGRRLVETHFANFDADEELVKISDEVIYRALYSYMKHEGTNMFETYQDRPHAYGHTWSPRFEP
VVKLMHGHAVTIGMSLGATLALEMGWITTNERDRIVNLCHSMGLSVYHEIIEDTELMLQAQKNITRKRGRRLWAPLPTGI
GSCSFAKEISPDLLTSAVEEHKKFCASFTSFGSQGKGEEMYLSDLGLE

Specific function: C7-cyclitol synthase using sedo-heptulose 7-phosphate, but not ido-heptulose 7-phosphate and 3-deoxy-arabino- heptulosonate 7-phosphate, as a substrate. Involved in the biosynthesis of the acarviose moiety of the alpha-glucosidase inhibitor acarbose [H]

COG id: COG0337

COG function: function code E; 3-dehydroquinate synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dehydroquinate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1789791, Length=347, Percent_Identity=24.207492795389, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320332, Length=271, Percent_Identity=26.9372693726937, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002658 [H]

Pfam domain/function: PF01761 DHQ_synthase [H]

EC number: 4.2.3.4

Molecular weight: Translated: 50324; Mature: 50193

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNAISTSVPVIEKVNKPDFVFDPSIRRDHAEQYRTSEWYDGRGEIKAVDDGRSFEISAT
CCCCCCCCCCHHHHCCCCCEEECCCHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEEEEE
FTLKSEVKLVNGVLDPSNPILANIYKSRNRCVAVVDHMVDELYGKSLTNYFEQNDIPLKT
EEEHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
LVCRAWESDKNTDTVHKILQFLGKDGCDVSRNEPVLIIGGGVLSDVAGLACSLQHRRTPY
HHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCE
VMIGTSVVAAIDAGPSPRTCTNSKLFKNGIGAYHPPVMTIVDRTLFRTLAKGHIRNGMAE
EEECCHHEEEECCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IIKMAVTDDQTLFELLEKYGRRLVETHFANFDADEELVKISDEVIYRALYSYMKHEGTNM
HHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCH
FETYQDRPHAYGHTWSPRFEPVVKLMHGHAVTIGMSLGATLALEMGWITTNERDRIVNLC
HHHHCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEHHCCCEEEEEECEECCCCHHHHHHHH
HSMGLSVYHEIIEDTELMLQAQKNITRKRGRRLWAPLPTGIGSCSFAKEISPDLLTSAVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCHHHHCCHHHHHHHHH
EHKKFCASFTSFGSQGKGEEMYLSDLGLE
HHHHHHHHHHHHCCCCCCCEEEHHHCCCC
>Mature Secondary Structure 
SNAISTSVPVIEKVNKPDFVFDPSIRRDHAEQYRTSEWYDGRGEIKAVDDGRSFEISAT
CCCCCCCCCHHHHCCCCCEEECCCHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEEEEE
FTLKSEVKLVNGVLDPSNPILANIYKSRNRCVAVVDHMVDELYGKSLTNYFEQNDIPLKT
EEEHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
LVCRAWESDKNTDTVHKILQFLGKDGCDVSRNEPVLIIGGGVLSDVAGLACSLQHRRTPY
HHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCE
VMIGTSVVAAIDAGPSPRTCTNSKLFKNGIGAYHPPVMTIVDRTLFRTLAKGHIRNGMAE
EEECCHHEEEECCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IIKMAVTDDQTLFELLEKYGRRLVETHFANFDADEELVKISDEVIYRALYSYMKHEGTNM
HHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCH
FETYQDRPHAYGHTWSPRFEPVVKLMHGHAVTIGMSLGATLALEMGWITTNERDRIVNLC
HHHHCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEHHCCCEEEEEECEECCCCHHHHHHHH
HSMGLSVYHEIIEDTELMLQAQKNITRKRGRRLWAPLPTGIGSCSFAKEISPDLLTSAVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCHHHHCCHHHHHHHHH
EHKKFCASFTSFGSQGKGEEMYLSDLGLE
HHHHHHHHHHHHCCCCCCCEEEHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: Co2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.033 {3-deoxy-D-arabino-heptulosonic} [C]

Substrates: 3-deoxy-arabino-heptulonate 7-phosphate

Specific reaction: 3-deoxy-arabino-heptulonate 7-phosphate = 3-dehydroquinate + phosphate

General reaction: elimination (P-O bond cleavage [C]

Inhibitor: 3-deoxy-D-arabino-heoptulosonic acid; 3-deoxy-D-arabino-heptulosonic acid7-homophosphate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10196166 [H]