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The map label for this gene is thiC

Identifier: 113476087

GI number: 113476087

Start: 3791090

End: 3792550

Strand: Reverse

Name: thiC

Synonym: Tery_2464

Alternate gene names: 113476087

Gene position: 3792550-3791090 (Counterclockwise)

Preceding gene: 113476088

Following gene: 113476086

Centisome position: 48.94

GC content: 41.14

Gene sequence:

>1461_bases
ATGCGGACAGAATGGATCGCCAAACGTAGTGGACACAAAAATGTCTCTCAGATGCACTACGGTCGTCAGGGAATCATCAC
TGAAGAAATGAACTTTGTGGCCCAACGAGAAAATCTTCCTGCTGAGTTAATTCGTGCAGAAGTAGCAAGGGGACGAATGA
TTATCCCTGCGAATATTAACCACACTAACCTTGAACCAATGTGTATTGGAATTGCTTCTCGGTGTAAAGTTAATGCCAAC
ATTGGCGCCTCTCCCAATACTTCTGATATTACTAAGGAAGTTGATAAACTAAAACTATCCATTAAATATGGTGCAGATAC
CGTGATGGACTTATCCACAGGTGGCGGTAACTTGGATGAAATTAGAACTGCTATTATCAACGCTTCCCCAGTACCTATCG
GAACAGTACCAGTATACCAAGCAGTAGAAAGCGTTCATGGCAGAATAGAAAATCTGACTGCTGATGACTTTCTTCATGTT
ATTGAAAAGCATGCTCAACAAGGGGTAGACTATCAAACCATCCACGCCGGAATTTTAATTGAACATCTACCTCTAGTCAG
AAGCCGTATCACTGGTATAGTTTCCCGTGGCGGTGGAATTATCGCCAAGTGGATGCTCCACCATCACAAACAAAATCCTC
TTTATACTCATTTTGACGATATTATTGAAATCTTCAAAAAATATGATGTTTCCTTTAGTTTAGGAGATTCTCTACGACCC
GGTTGTACCCATGATGCTTCTGATGAAGCCCAACTAGCAGAATTAAAAACATTAGGTCAGTTAACTCGCCGTGCCTGGGG
ACATGAAGTTCAAGTTATGGTAGAAGGTCCTGGTCATGTACCAATGGACCAAATTGAGTTTAATGTGAAAAAACAAATGG
AAGAGTGTTCAAGTCCTCCTTTGAATGCTTTGGATCTAGATCCCTTAGCATCCAACGCCAATGAACAGATGGAAAAGTTT
AATGTTCTAGGTCCAGCTCCTTTCTATGTTTTAGGTCCATTAGTCACAGATATTGCTCCTGGTTATGATCATATTACTTC
TGCTATTGGTGCAGCAATGGCGGGATGGTATGGTACAGCAATGTTATGTTACGTTACTCCGAAAGAACATTTAGGTTTGC
CTAACGCTGAGGATGTCAGAAATGGACTCATTGCTTATAAAATTGCAGCCCATGCAGCAGATATTGGTCGGCATCGTCAG
GGAGCGAGGGATAGAGATGATGAACTTTCTGCTGCTCGTTACAATTTTGATTGGAACCGTCAATTTGAGTTATCTTTAGA
TCCAGAGCGAGCCAAAGAATATCACGATGAAACTTTGCCTGCGGATATCTATAAAACTGCTGAATTTTGTTCTATGTGCG
GACCAAAATTCTGTCCGATGCAAACTAAGGTTGATGCTGACGCTTTGACAGAATTAGAGAAGTTTTTAGCCAAGGAAAAA
GAAGTTGTAACTCAAAGTTAA

Upstream 100 bases:

>100_bases
TAAGCTAGGGGTGCCTAAAATATTTAGGCTGAGAAATACCCTTAGAACCTGAGACTGGTTAATACCAGCGGAGGGAAGCT
GTTTATTTAAGGAAAAAAAT

Downstream 100 bases:

>100_bases
TAGACATATTAATTTTAAAATAATTAGGGTGGGGTAGAAAACAATTAATATTTGGGTTTAATTGTTAATCTTTGTATTTT
GTGTGTTCCTGCTTTCTGGA

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MRTEWIAKRSGHKNVSQMHYGRQGIITEEMNFVAQRENLPAELIRAEVARGRMIIPANINHTNLEPMCIGIASRCKVNAN
IGASPNTSDITKEVDKLKLSIKYGADTVMDLSTGGGNLDEIRTAIINASPVPIGTVPVYQAVESVHGRIENLTADDFLHV
IEKHAQQGVDYQTIHAGILIEHLPLVRSRITGIVSRGGGIIAKWMLHHHKQNPLYTHFDDIIEIFKKYDVSFSLGDSLRP
GCTHDASDEAQLAELKTLGQLTRRAWGHEVQVMVEGPGHVPMDQIEFNVKKQMEECSSPPLNALDLDPLASNANEQMEKF
NVLGPAPFYVLGPLVTDIAPGYDHITSAIGAAMAGWYGTAMLCYVTPKEHLGLPNAEDVRNGLIAYKIAAHAADIGRHRQ
GARDRDDELSAARYNFDWNRQFELSLDPERAKEYHDETLPADIYKTAEFCSMCGPKFCPMQTKVDADALTELEKFLAKEK
EVVTQS

Sequences:

>Translated_486_residues
MRTEWIAKRSGHKNVSQMHYGRQGIITEEMNFVAQRENLPAELIRAEVARGRMIIPANINHTNLEPMCIGIASRCKVNAN
IGASPNTSDITKEVDKLKLSIKYGADTVMDLSTGGGNLDEIRTAIINASPVPIGTVPVYQAVESVHGRIENLTADDFLHV
IEKHAQQGVDYQTIHAGILIEHLPLVRSRITGIVSRGGGIIAKWMLHHHKQNPLYTHFDDIIEIFKKYDVSFSLGDSLRP
GCTHDASDEAQLAELKTLGQLTRRAWGHEVQVMVEGPGHVPMDQIEFNVKKQMEECSSPPLNALDLDPLASNANEQMEKF
NVLGPAPFYVLGPLVTDIAPGYDHITSAIGAAMAGWYGTAMLCYVTPKEHLGLPNAEDVRNGLIAYKIAAHAADIGRHRQ
GARDRDDELSAARYNFDWNRQFELSLDPERAKEYHDETLPADIYKTAEFCSMCGPKFCPMQTKVDADALTELEKFLAKEK
EVVTQS
>Mature_486_residues
MRTEWIAKRSGHKNVSQMHYGRQGIITEEMNFVAQRENLPAELIRAEVARGRMIIPANINHTNLEPMCIGIASRCKVNAN
IGASPNTSDITKEVDKLKLSIKYGADTVMDLSTGGGNLDEIRTAIINASPVPIGTVPVYQAVESVHGRIENLTADDFLHV
IEKHAQQGVDYQTIHAGILIEHLPLVRSRITGIVSRGGGIIAKWMLHHHKQNPLYTHFDDIIEIFKKYDVSFSLGDSLRP
GCTHDASDEAQLAELKTLGQLTRRAWGHEVQVMVEGPGHVPMDQIEFNVKKQMEECSSPPLNALDLDPLASNANEQMEKF
NVLGPAPFYVLGPLVTDIAPGYDHITSAIGAAMAGWYGTAMLCYVTPKEHLGLPNAEDVRNGLIAYKIAAHAADIGRHRQ
GARDRDDELSAARYNFDWNRQFELSLDPERAKEYHDETLPADIYKTAEFCSMCGPKFCPMQTKVDADALTELEKFLAKEK
EVVTQS

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=476, Percent_Identity=55.0420168067227, Blast_Score=517, Evalue=1e-148,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_TRIEI (Q111Z9)

Other databases:

- EMBL:   CP000393
- RefSeq:   YP_722148.1
- STRING:   Q111Z9
- GeneID:   4244649
- GenomeReviews:   CP000393_GR
- KEGG:   ter:Tery_2464
- NMPDR:   fig|203124.1.peg.1264
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   TWELFRD
- PhylomeDB:   Q111Z9
- ProtClustDB:   PRK13352
- BioCyc:   TERY203124:TERY_2464-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 53898; Mature: 53898

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: BINDING 80-80 BINDING 109-109 BINDING 139-139 BINDING 175-175 BINDING 275-275 BINDING 329-329

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTEWIAKRSGHKNVSQMHYGRQGIITEEMNFVAQRENLPAELIRAEVARGRMIIPANIN
CCCCHHHHCCCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCC
HTNLEPMCIGIASRCKVNANIGASPNTSDITKEVDKLKLSIKYGADTVMDLSTGGGNLDE
CCCCCHHEEEHHHHCEECCCCCCCCCHHHHHHHHHHEEEEEEECCCEEEEECCCCCCHHH
IRTAIINASPVPIGTVPVYQAVESVHGRIENLTADDFLHVIEKHAQQGVDYQTIHAGILI
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEHHHHHHHH
EHLPLVRSRITGIVSRGGGIIAKWMLHHHKQNPLYTHFDDIIEIFKKYDVSFSLGDSLRP
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEECCCCCCC
GCTHDASDEAQLAELKTLGQLTRRAWGHEVQVMVEGPGHVPMDQIEFNVKKQMEECSSPP
CCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCC
LNALDLDPLASNANEQMEKFNVLGPAPFYVLGPLVTDIAPGYDHITSAIGAAMAGWYGTA
CCCCCCCHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCE
MLCYVTPKEHLGLPNAEDVRNGLIAYKIAAHAADIGRHRQGARDRDDELSAARYNFDWNR
EEEEECCHHHCCCCCHHHHHCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCE
QFELSLDPERAKEYHDETLPADIYKTAEFCSMCGPKFCPMQTKVDADALTELEKFLAKEK
EEEEECCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
EVVTQS
HHHCCC
>Mature Secondary Structure
MRTEWIAKRSGHKNVSQMHYGRQGIITEEMNFVAQRENLPAELIRAEVARGRMIIPANIN
CCCCHHHHCCCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCC
HTNLEPMCIGIASRCKVNANIGASPNTSDITKEVDKLKLSIKYGADTVMDLSTGGGNLDE
CCCCCHHEEEHHHHCEECCCCCCCCCHHHHHHHHHHEEEEEEECCCEEEEECCCCCCHHH
IRTAIINASPVPIGTVPVYQAVESVHGRIENLTADDFLHVIEKHAQQGVDYQTIHAGILI
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEHHHHHHHH
EHLPLVRSRITGIVSRGGGIIAKWMLHHHKQNPLYTHFDDIIEIFKKYDVSFSLGDSLRP
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEECCCCCCC
GCTHDASDEAQLAELKTLGQLTRRAWGHEVQVMVEGPGHVPMDQIEFNVKKQMEECSSPP
CCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCC
LNALDLDPLASNANEQMEKFNVLGPAPFYVLGPLVTDIAPGYDHITSAIGAAMAGWYGTA
CCCCCCCHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCE
MLCYVTPKEHLGLPNAEDVRNGLIAYKIAAHAADIGRHRQGARDRDDELSAARYNFDWNR
EEEEECCHHHCCCCCHHHHHCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCE
QFELSLDPERAKEYHDETLPADIYKTAEFCSMCGPKFCPMQTKVDADALTELEKFLAKEK
EEEEECCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
EVVTQS
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA