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The map label for this gene is leuA

Identifier: 113475894

GI number: 113475894

Start: 3503654

End: 3505279

Strand: Reverse

Name: leuA

Synonym: Tery_2253

Alternate gene names: 113475894

Gene position: 3505279-3503654 (Counterclockwise)

Preceding gene: 113475899

Following gene: 113475893

Centisome position: 45.23

GC content: 41.33

Gene sequence:

>1626_bases
ATGATCACCAGTCAAGACCGAGTTATTATTTTTGATACTACCCTCAGGGATGGAGAACAATCTCCAGGAGCAACTCTCAA
TATAGATGAAAAATTGACCATTGCTCGACAACTATCTCTTTTGGGAGTAGACGTCATTGAAGCTGGTTTCCCCTATGCTA
GTATTGGGGATTTTGAGGCAGTACAAAAAATTGCTGAGATAGTGGGAGTAGAAGGAGGCCCAACTATTTGCGGTTTGGCA
AGAACAACTCGTGCAGATATTGAGGCAGCAGGTAAAGCCCTTAAACCAGCAGCTAAAGCGCGTATTCATACCTTTATTGC
TACCTCTGATATTCACTTAGCTTACAAACTGAAGAAAACTAGAGCAGAAGTATTGGAGATTGCTTCAGAAATGGTTGCCT
ATGCTAAAACCTTTGTAGACGATGTAGAGTTTTCTCCGGAAGATGCGGGTCGCTCTGACCCAGAATTTCTTTATCAAGTA
TTGGAAAAGGCGATCGCTGCTGGAGCTACTACTGTGAATATTCCTGATACTGTAGGTTATACAACTCCTGCTGAGTTTGG
GGGGCTGATCCGGGGTATTAAAGAAAATGTACCAAATATTGACCAAGCTATTATTTCAGTTCACGGTCACAATGATTTGG
GTTTGGCAGTCGCTAATTTTCTGGAAGCAGTGAAAAATGGGGCTCGTCAGTTAGAATGTACTATCAATGGCATTGGGGAA
CGTGCTGGTAATGCTGCTTTAGAAGAATTGGTAATGGCGTTTCACGTTCGGCGACAATATTTCAACCCATTTTTTGGTCG
CCCACCAGAGTCAGAAACACCACTAACTAATATTAATACTCGTCATATTTACAAAACATCTCGTTTGGTTTCTAGTTTGA
CAGGAATGTTCGTGCAACCAAATAAAGCTATTGTTGGTGCTAATGCTTTTGCTCACGAGTCAGGTATTCACCAAGATGGG
GTACTCAAAAATAAACTGACCTATGAAATTATGGATGCTCAGTCAATTGGGTTGACAGATAATCAGATTGTTTTGGGTAA
ACTTTCGGGTCGTCATGCTTTCCAAAGTAGGTTGCAAGAGCTTGGGTTTAAACTTTCAGATGAAGAAATTAATAAGGCGT
TTATCAAGTTCAAAGACTTGGCAGATAAAAAGAAAGAAATAACAGATTGGGATTTGGAATCTATTGTTAATGCTGAAATT
CAACAACCACCGGAACTATTCCGTTTGGAGTTGGTGCAGGTTTCTTGTGGCGATCGCTCTCGCCCTACAGCTACAGTTAT
TATCCGCACTCCCAATGGGGAAGAATTAACTGATGCTGCCATTGGTACTGGTCCTGTAGATGCTGTGTACAAAGCAATTA
ACCGAGTGGTGAATGTAGAAAATGATTTAGTTGAGTTTTCTGTGCAGTCTGTAACTGCTGGTATTGATGCTATTGGGGAA
GTCACTATTCGCGTGCGCCATGAAGGGCGGGTTTATTCTGGTCATGCTGCTAATACTGATATTATTGTGGCATCTGCTGA
GGCTTATCTAAGTGCTTTAAATCGGTTGTATGCAGTTTTGCAAAGAGGTGTAGAGAAAATAAATTTACCAAAGGATCAAC
CTACGGAAGTAGTAGCTGGTAGTTGA

Upstream 100 bases:

>100_bases
TAACTTTTATAGTCGGTCGCTTGTGTAGGGACTTGAAAAGAAATTGCATAAAATAAAGCCAACTTCCCTTTAACATTATA
GAGTAAAAGAAGAAAAAGCA

Downstream 100 bases:

>100_bases
CTAGCTTAAAACTTATTGTAGAAACCGGGTTTATTAACAAAATTTCTTGATTTGAACAATAATAATTAGGGCAATAAACT
CGGTAAAACCTACTAAAACT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MITSQDRVIIFDTTLRDGEQSPGATLNIDEKLTIARQLSLLGVDVIEAGFPYASIGDFEAVQKIAEIVGVEGGPTICGLA
RTTRADIEAAGKALKPAAKARIHTFIATSDIHLAYKLKKTRAEVLEIASEMVAYAKTFVDDVEFSPEDAGRSDPEFLYQV
LEKAIAAGATTVNIPDTVGYTTPAEFGGLIRGIKENVPNIDQAIISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE
RAGNAALEELVMAFHVRRQYFNPFFGRPPESETPLTNINTRHIYKTSRLVSSLTGMFVQPNKAIVGANAFAHESGIHQDG
VLKNKLTYEIMDAQSIGLTDNQIVLGKLSGRHAFQSRLQELGFKLSDEEINKAFIKFKDLADKKKEITDWDLESIVNAEI
QQPPELFRLELVQVSCGDRSRPTATVIIRTPNGEELTDAAIGTGPVDAVYKAINRVVNVENDLVEFSVQSVTAGIDAIGE
VTIRVRHEGRVYSGHAANTDIIVASAEAYLSALNRLYAVLQRGVEKINLPKDQPTEVVAGS

Sequences:

>Translated_541_residues
MITSQDRVIIFDTTLRDGEQSPGATLNIDEKLTIARQLSLLGVDVIEAGFPYASIGDFEAVQKIAEIVGVEGGPTICGLA
RTTRADIEAAGKALKPAAKARIHTFIATSDIHLAYKLKKTRAEVLEIASEMVAYAKTFVDDVEFSPEDAGRSDPEFLYQV
LEKAIAAGATTVNIPDTVGYTTPAEFGGLIRGIKENVPNIDQAIISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE
RAGNAALEELVMAFHVRRQYFNPFFGRPPESETPLTNINTRHIYKTSRLVSSLTGMFVQPNKAIVGANAFAHESGIHQDG
VLKNKLTYEIMDAQSIGLTDNQIVLGKLSGRHAFQSRLQELGFKLSDEEINKAFIKFKDLADKKKEITDWDLESIVNAEI
QQPPELFRLELVQVSCGDRSRPTATVIIRTPNGEELTDAAIGTGPVDAVYKAINRVVNVENDLVEFSVQSVTAGIDAIGE
VTIRVRHEGRVYSGHAANTDIIVASAEAYLSALNRLYAVLQRGVEKINLPKDQPTEVVAGS
>Mature_541_residues
MITSQDRVIIFDTTLRDGEQSPGATLNIDEKLTIARQLSLLGVDVIEAGFPYASIGDFEAVQKIAEIVGVEGGPTICGLA
RTTRADIEAAGKALKPAAKARIHTFIATSDIHLAYKLKKTRAEVLEIASEMVAYAKTFVDDVEFSPEDAGRSDPEFLYQV
LEKAIAAGATTVNIPDTVGYTTPAEFGGLIRGIKENVPNIDQAIISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE
RAGNAALEELVMAFHVRRQYFNPFFGRPPESETPLTNINTRHIYKTSRLVSSLTGMFVQPNKAIVGANAFAHESGIHQDG
VLKNKLTYEIMDAQSIGLTDNQIVLGKLSGRHAFQSRLQELGFKLSDEEINKAFIKFKDLADKKKEITDWDLESIVNAEI
QQPPELFRLELVQVSCGDRSRPTATVIIRTPNGEELTDAAIGTGPVDAVYKAINRVVNVENDLVEFSVQSVTAGIDAIGE
VTIRVRHEGRVYSGHAANTDIIVASAEAYLSALNRLYAVLQRGVEKINLPKDQPTEVVAGS

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=513, Percent_Identity=50.4873294346979, Blast_Score=470, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6320071, Length=406, Percent_Identity=30.0492610837438, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6320019, Length=437, Percent_Identity=28.8329519450801, Blast_Score=147, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6324225, Length=524, Percent_Identity=23.6641221374046, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324682, Length=524, Percent_Identity=23.2824427480916, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_TRIEI (Q112U2)

Other databases:

- EMBL:   CP000393
- RefSeq:   YP_721955.1
- ProteinModelPortal:   Q112U2
- SMR:   Q112U2
- STRING:   Q112U2
- GeneID:   4243346
- GenomeReviews:   CP000393_GR
- KEGG:   ter:Tery_2253
- NMPDR:   fig|203124.1.peg.1462
- eggNOG:   COG0119
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- PhylomeDB:   Q112U2
- ProtClustDB:   PRK00915
- BioCyc:   TERY203124:TERY_2253-MONOMER
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 58747; Mature: 58747

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITSQDRVIIFDTTLRDGEQSPGATLNIDEKLTIARQLSLLGVDVIEAGFPYASIGDFEA
CCCCCCCEEEEECCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
VQKIAEIVGVEGGPTICGLARTTRADIEAAGKALKPAAKARIHTFIATSDIHLAYKLKKT
HHHHHHHHCCCCCCCEEECHHHHHHHHHHHHHHHCHHHHHHEEEEEEECCEEEEEEHHHH
RAEVLEIASEMVAYAKTFVDDVEFSPEDAGRSDPEFLYQVLEKAIAAGATTVNIPDTVGY
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCC
TTPAEFGGLIRGIKENVPNIDQAIISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE
CCCHHHHHHHHHHHHCCCCHHHHHEEECCCCCHHHHHHHHHHHHHCCCEEEEEEEECHHH
RAGNAALEELVMAFHVRRQYFNPFFGRPPESETPLTNINTRHIYKTSRLVSSLTGMFVQP
HCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEECC
NKAIVGANAFAHESGIHQDGVLKNKLTYEIMDAQSIGLTDNQIVLGKLSGRHAFQSRLQE
CCEEEECCHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHH
LGFKLSDEEINKAFIKFKDLADKKKEITDWDLESIVNAEIQQPPELFRLELVQVSCGDRS
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHEEEEEEEEECCCCC
RPTATVIIRTPNGEELTDAAIGTGPVDAVYKAINRVVNVENDLVEFSVQSVTAGIDAIGE
CCEEEEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCE
VTIRVRHEGRVYSGHAANTDIIVASAEAYLSALNRLYAVLQRGVEKINLPKDQPTEVVAG
EEEEEEECCEEEECCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
S
C
>Mature Secondary Structure
MITSQDRVIIFDTTLRDGEQSPGATLNIDEKLTIARQLSLLGVDVIEAGFPYASIGDFEA
CCCCCCCEEEEECCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
VQKIAEIVGVEGGPTICGLARTTRADIEAAGKALKPAAKARIHTFIATSDIHLAYKLKKT
HHHHHHHHCCCCCCCEEECHHHHHHHHHHHHHHHCHHHHHHEEEEEEECCEEEEEEHHHH
RAEVLEIASEMVAYAKTFVDDVEFSPEDAGRSDPEFLYQVLEKAIAAGATTVNIPDTVGY
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCC
TTPAEFGGLIRGIKENVPNIDQAIISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE
CCCHHHHHHHHHHHHCCCCHHHHHEEECCCCCHHHHHHHHHHHHHCCCEEEEEEEECHHH
RAGNAALEELVMAFHVRRQYFNPFFGRPPESETPLTNINTRHIYKTSRLVSSLTGMFVQP
HCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEECC
NKAIVGANAFAHESGIHQDGVLKNKLTYEIMDAQSIGLTDNQIVLGKLSGRHAFQSRLQE
CCEEEECCHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHH
LGFKLSDEEINKAFIKFKDLADKKKEITDWDLESIVNAEIQQPPELFRLELVQVSCGDRS
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHEEEEEEEEECCCCC
RPTATVIIRTPNGEELTDAAIGTGPVDAVYKAINRVVNVENDLVEFSVQSVTAGIDAIGE
CCEEEEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCE
VTIRVRHEGRVYSGHAANTDIIVASAEAYLSALNRLYAVLQRGVEKINLPKDQPTEVVAG
EEEEEEECCEEEECCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA