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The map label for this gene is aniA [H]

Identifier: 113474657

GI number: 113474657

Start: 1318857

End: 1319852

Strand: Reverse

Name: aniA [H]

Synonym: Tery_0831

Alternate gene names: 113474657

Gene position: 1319852-1318857 (Counterclockwise)

Preceding gene: 113474658

Following gene: 113474656

Centisome position: 17.03

GC content: 39.36

Gene sequence:

>996_bases
ATGGCCAATCTTCCTCTTCCAAAGCCAGAAAAATCGGAATTTTGGAACAGACGGCAATTCCTAAAGTTAGGAATAGTTGG
TACTGGTGTCACTTTAGCTGGCATCAGTACTCAACATATTCTACCTTTATTGTCGTCATCACTTAAGGCCCAGGCTGCCA
TAAATGCCATAGAACTAGATCCTATGAATACTCTGCGGGATTTTGATTATGGCATGCTCAAAGAAGAAAATGGTCGCAAA
ATCAGGGAATTTCAGATTAAAGCAATTAATTCTAAATTACAACTAAATCAAGCAGTATCCTTAATTAGTTGGAACTATAA
TGGTCGTGTTCCTGGGCCAACACTGAGAGCAACAGCAGGGGAGCGAGTACGAATTATTTTTGAGAATGAAGGTGGTCATG
CTCATACGATGCACTTTCACGGTATTCATCCAGCAGAAGCAGATGGAGTACGACCAGTACGCCATGGCAAAACAACAGTT
TATGAATTTGATGCTCGACCTTTTGGGGTTCATCTCTATCATTGCCATATTACTCCAGTGACTCGTCATATTAGCAAAGG
GTTGTACGGTATGTTTATTATCGATCCTCCCGAAGGTCGTCCCCCTGCTGATGAGATAGTATTGGTAATGGGAGGTTATG
ATACTAACAATGATGGCAAAAATGAGCTTTATGCTTTTAATGGTTTGCCAAACTATTACAAAACCCATCCAATACTGATA
CAGCAAAATAAACTGGTGCGTTTGTATATACTGAATATGATTGAGTTTGACTCTGCTGCAACATTTCATATTCATGGAAA
TTTTTTCCAGGTTTATCCTACTGGTATGACTGTTAAACCATGGCAAAAAACAGATGTAGTTACTATGGGAACTTCTGAAA
GACATATTCTCGAATTTTACTATTCCTTCCCTGGTAAATATATGTTTCATCCTCACCAAGATGCGATCGCGGAAAGTGGT
TGTATTGGTTTATTTGAGGTTTTGCCTCAGGCATAA

Upstream 100 bases:

>100_bases
CCATAAGTTTTGGACTTACCTAATATAGATAAAAACTTCAATAAGCTATATAATAGTTTTCAGTTTATTAAAAATAATTA
TCAATAATTGTGAGAGAGCT

Downstream 100 bases:

>100_bases
AAATTTGAGCAAATTATTAACTGAATTTGATGGTTATTAATTATGATGAATATTAACACTATTTTACGTTATTTTTTGGT
GGGATTTCTTGCTACTTTAT

Product: twin-arginine translocation pathway signal protein

Products: NA

Alternate protein names: Major outer membrane protein Pan 1 [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MANLPLPKPEKSEFWNRRQFLKLGIVGTGVTLAGISTQHILPLLSSSLKAQAAINAIELDPMNTLRDFDYGMLKEENGRK
IREFQIKAINSKLQLNQAVSLISWNYNGRVPGPTLRATAGERVRIIFENEGGHAHTMHFHGIHPAEADGVRPVRHGKTTV
YEFDARPFGVHLYHCHITPVTRHISKGLYGMFIIDPPEGRPPADEIVLVMGGYDTNNDGKNELYAFNGLPNYYKTHPILI
QQNKLVRLYILNMIEFDSAATFHIHGNFFQVYPTGMTVKPWQKTDVVTMGTSERHILEFYYSFPGKYMFHPHQDAIAESG
CIGLFEVLPQA

Sequences:

>Translated_331_residues
MANLPLPKPEKSEFWNRRQFLKLGIVGTGVTLAGISTQHILPLLSSSLKAQAAINAIELDPMNTLRDFDYGMLKEENGRK
IREFQIKAINSKLQLNQAVSLISWNYNGRVPGPTLRATAGERVRIIFENEGGHAHTMHFHGIHPAEADGVRPVRHGKTTV
YEFDARPFGVHLYHCHITPVTRHISKGLYGMFIIDPPEGRPPADEIVLVMGGYDTNNDGKNELYAFNGLPNYYKTHPILI
QQNKLVRLYILNMIEFDSAATFHIHGNFFQVYPTGMTVKPWQKTDVVTMGTSERHILEFYYSFPGKYMFHPHQDAIAESG
CIGLFEVLPQA
>Mature_330_residues
ANLPLPKPEKSEFWNRRQFLKLGIVGTGVTLAGISTQHILPLLSSSLKAQAAINAIELDPMNTLRDFDYGMLKEENGRKI
REFQIKAINSKLQLNQAVSLISWNYNGRVPGPTLRATAGERVRIIFENEGGHAHTMHFHGIHPAEADGVRPVRHGKTTVY
EFDARPFGVHLYHCHITPVTRHISKGLYGMFIIDPPEGRPPADEIVLVMGGYDTNNDGKNELYAFNGLPNYYKTHPILIQ
QNKLVRLYILNMIEFDSAATFHIHGNFFQVYPTGMTVKPWQKTDVVTMGTSERHILEFYYSFPGKYMFHPHQDAIAESGC
IGLFEVLPQA

Specific function: Catalyzes the reduction of nitrite to nitric oxide (NO), probably with azurin as electron donor. Essential for growth and survival in oxygen-depleted environments. Can also provide protection against killing by normal human sera [H]

COG id: COG2132

COG function: function code Q; Putative multicopper oxidases

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 plastocyanin-like domains [H]

Homologues:

None

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001117
- InterPro:   IPR011707
- InterPro:   IPR008972
- InterPro:   IPR001287 [H]

Pfam domain/function: PF00394 Cu-oxidase; PF07732 Cu-oxidase_3 [H]

EC number: =1.7.2.1 [H]

Molecular weight: Translated: 37397; Mature: 37266

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANLPLPKPEKSEFWNRRQFLKLGIVGTGVTLAGISTQHILPLLSSSLKAQAAINAIELD
CCCCCCCCCCHHHHHCCCEEEEEEEEECCEEEECCCHHHHHHHHHHHHHHHHHEEEEEEC
PMNTLRDFDYGMLKEENGRKIREFQIKAINSKLQLNQAVSLISWNYNGRVPGPTLRATAG
CCHHHHHCCCCCEECCCCCEEEEEEEEECCCHHHHCCEEEEEEECCCCCCCCCEEEECCC
ERVRIIFENEGGHAHTMHFHGIHPAEADGVRPVRHGKTTVYEFDARPFGVHLYHCHITPV
CEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEEEEECHH
TRHISKGLYGMFIIDPPEGRPPADEIVLVMGGYDTNNDGKNELYAFNGLPNYYKTHPILI
HHHHHCCCEEEEEEECCCCCCCHHHEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCEEE
QQNKLVRLYILNMIEFDSAATFHIHGNFFQVYPTGMTVKPWQKTDVVTMGTSERHILEFY
ECCCEEEEEEEEEEECCCEEEEEEECCEEEEEECCEEECCCCCCCEEEECCCCCEEEEEE
YSFPGKYMFHPHQDAIAESGCIGLFEVLPQA
ECCCCCEEECCCHHHHHCCCCEEHHHHCCCC
>Mature Secondary Structure 
ANLPLPKPEKSEFWNRRQFLKLGIVGTGVTLAGISTQHILPLLSSSLKAQAAINAIELD
CCCCCCCCCHHHHHCCCEEEEEEEEECCEEEECCCHHHHHHHHHHHHHHHHHEEEEEEC
PMNTLRDFDYGMLKEENGRKIREFQIKAINSKLQLNQAVSLISWNYNGRVPGPTLRATAG
CCHHHHHCCCCCEECCCCCEEEEEEEEECCCHHHHCCEEEEEEECCCCCCCCCEEEECCC
ERVRIIFENEGGHAHTMHFHGIHPAEADGVRPVRHGKTTVYEFDARPFGVHLYHCHITPV
CEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEEEEECHH
TRHISKGLYGMFIIDPPEGRPPADEIVLVMGGYDTNNDGKNELYAFNGLPNYYKTHPILI
HHHHHCCCEEEEEEECCCCCCCHHHEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCEEE
QQNKLVRLYILNMIEFDSAATFHIHGNFFQVYPTGMTVKPWQKTDVVTMGTSERHILEFY
ECCCEEEEEEEEEEECCCEEEEEEECCEEEEEECCEEECCCCCCCEEEECCCCCEEEEEE
YSFPGKYMFHPHQDAIAESGCIGLFEVLPQA
ECCCCCEEECCCHHHHHCCCCEEHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1383156; 1398981 [H]