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The map label for this gene is mgsA [C]

Identifier: 113474653

GI number: 113474653

Start: 1312665

End: 1313972

Strand: Reverse

Name: mgsA [C]

Synonym: Tery_0827

Alternate gene names: 113474653

Gene position: 1313972-1312665 (Counterclockwise)

Preceding gene: 113474656

Following gene: 113474651

Centisome position: 16.95

GC content: 39.07

Gene sequence:

>1308_bases
ATGGGAAATACTATTGCTTTACTAGCTTATGACAGTAAAAAAGATAACTTAGTTGAATTTGTCAAAAAACATAAAATTGT
ACTATCTCGCTATCAATTAGTAGCGACAGAAAGCACTGCCCAATATATTTTACAAGAGGTAGATATAGAGATAAATTCTG
TCTTGGCAGGCCCCTTGGGAGGAGATGCTCAATTAGCTGTAGAAGTAACTACGGGTAATGTGGTTGCTGTTATTTGTTTG
GTGGACTACTTATTTTCTCAGTCTTATGAACCTGATATCAAATTATTATTGCGTCTTTGTCAGGTGCATAATGTTCCTAT
TGCGATCAATATTGCTACGGCGGAAATTATTATTAAGGGTTTTATTCAAACTACTGTTGCTCACCTCATATTTAACCCTA
TATCTGGTAAGGGCAATGCTCAACAAGATTTATTATTAATTAAATCACTTCTCGAACCTCATCTACATTTAGATATACAT
CAAACTACCCCAGAAAAAACTGCAGAAGAATTAACGGAGTTAGCGATCGCTAAACAAGCAGATATAATTATTGCTTGTGG
AGGAGATGGGACAGTTTCTGCTGTTGCTGGGGCTTTAGTAAATACAAATATCCCTCTGGGTATAATTCCGAGGGGAACAG
CTAATGCGTTTTGTATGGCATTAGGCATTCCCAGTATTGCGCCGATCAGAGGTGCCTGTGATCTAATCTTGGCAGGGAAA
ACTCGTGTTATAGATGTTGGTCGTTGCAATGAACGTACTATGATTTTATTAGCAGGGGTGGGATATGAAGCAGAAACTAT
AGAAAGAGCAGACCGGGAAGCTAAAAACCGTTGGGGAGTTTTGGCATATATGATGGCAGGTTGGCGGCAGCTCACCGAAC
AGCAACTTTTTGATATTGAAATTGAAATTGATGGTGTAGCTAAAACTTTCCAAGCTGGGGCTATTACTATTGCTAATGCA
GCTCCACCTACATCATTTTTAGCCCAAGGTTTGGGAAAGGTAATTATGAATGATGGTTTATTGGAAGTAATGATCATTAC
TTCTAAGAATACACTGCAGGCTGTTAATGCTGGAGTCAGAATGTTCGGCTCGGCTATCTTTCAAACTCCTACTCAACATG
AAGATACTATTGCTTTGCGTACAAAAAGGGTTAAAGTTAGTGCAGATCCACCCCAAAAAGTTGTGCTTGATGGTGAAATT
ATTGGCACTACTCCTATTGAGGTAGAGTGTATTCCTAATGGTTTAACTGTGTTGGCTCCACCAACTAATGAAGCTGAAGA
AAAGCCTGCCTGGGGCTTTTTCTCTTGA

Upstream 100 bases:

>100_bases
GTCTGTCAGAGATTATTTTTAACTTTTAATTTTTAATTTTTAATTTTAAACTTTTGAATTGCCCGTAGCACTATAGTCAG
TTAATTCTAGGATGAAAATT

Downstream 100 bases:

>100_bases
AATCAGGGGTTACGCAAAACCTCTCCCCTATTGGGTTGGCGTAACTCCTGTTATTGTTAACTAACCTATGTTATTTTTCT
AGTTATTTTCACCACATAAT

Product: methylglyoxal synthase

Products: Methylglyoxal; Phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MGNTIALLAYDSKKDNLVEFVKKHKIVLSRYQLVATESTAQYILQEVDIEINSVLAGPLGGDAQLAVEVTTGNVVAVICL
VDYLFSQSYEPDIKLLLRLCQVHNVPIAINIATAEIIIKGFIQTTVAHLIFNPISGKGNAQQDLLLIKSLLEPHLHLDIH
QTTPEKTAEELTELAIAKQADIIIACGGDGTVSAVAGALVNTNIPLGIIPRGTANAFCMALGIPSIAPIRGACDLILAGK
TRVIDVGRCNERTMILLAGVGYEAETIERADREAKNRWGVLAYMMAGWRQLTEQQLFDIEIEIDGVAKTFQAGAITIANA
APPTSFLAQGLGKVIMNDGLLEVMIITSKNTLQAVNAGVRMFGSAIFQTPTQHEDTIALRTKRVKVSADPPQKVVLDGEI
IGTTPIEVECIPNGLTVLAPPTNEAEEKPAWGFFS

Sequences:

>Translated_435_residues
MGNTIALLAYDSKKDNLVEFVKKHKIVLSRYQLVATESTAQYILQEVDIEINSVLAGPLGGDAQLAVEVTTGNVVAVICL
VDYLFSQSYEPDIKLLLRLCQVHNVPIAINIATAEIIIKGFIQTTVAHLIFNPISGKGNAQQDLLLIKSLLEPHLHLDIH
QTTPEKTAEELTELAIAKQADIIIACGGDGTVSAVAGALVNTNIPLGIIPRGTANAFCMALGIPSIAPIRGACDLILAGK
TRVIDVGRCNERTMILLAGVGYEAETIERADREAKNRWGVLAYMMAGWRQLTEQQLFDIEIEIDGVAKTFQAGAITIANA
APPTSFLAQGLGKVIMNDGLLEVMIITSKNTLQAVNAGVRMFGSAIFQTPTQHEDTIALRTKRVKVSADPPQKVVLDGEI
IGTTPIEVECIPNGLTVLAPPTNEAEEKPAWGFFS
>Mature_434_residues
GNTIALLAYDSKKDNLVEFVKKHKIVLSRYQLVATESTAQYILQEVDIEINSVLAGPLGGDAQLAVEVTTGNVVAVICLV
DYLFSQSYEPDIKLLLRLCQVHNVPIAINIATAEIIIKGFIQTTVAHLIFNPISGKGNAQQDLLLIKSLLEPHLHLDIHQ
TTPEKTAEELTELAIAKQADIIIACGGDGTVSAVAGALVNTNIPLGIIPRGTANAFCMALGIPSIAPIRGACDLILAGKT
RVIDVGRCNERTMILLAGVGYEAETIERADREAKNRWGVLAYMMAGWRQLTEQQLFDIEIEIDGVAKTFQAGAITIANAA
PPTSFLAQGLGKVIMNDGLLEVMIITSKNTLQAVNAGVRMFGSAIFQTPTQHEDTIALRTKRVKVSADPPQKVVLDGEII
GTTPIEVECIPNGLTVLAPPTNEAEEKPAWGFFS

Specific function: Unknown

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI87081809, Length=116, Percent_Identity=37.9310344827586, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1788402, Length=284, Percent_Identity=26.056338028169, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001206
- InterPro:   IPR004363
- InterPro:   IPR018148
- InterPro:   IPR011607 [H]

Pfam domain/function: PF00781 DAGK_cat; PF02142 MGS [H]

EC number: 4.2.3.3 [C]

Molecular weight: Translated: 46734; Mature: 46603

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNTIALLAYDSKKDNLVEFVKKHKIVLSRYQLVATESTAQYILQEVDIEINSVLAGPLG
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHEEEEEECHHHHHHHHHHCEEEEEEEECCCC
GDAQLAVEVTTGNVVAVICLVDYLFSQSYEPDIKLLLRLCQVHNVPIAINIATAEIIIKG
CCCEEEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEHHHHHHHH
FIQTTVAHLIFNPISGKGNAQQDLLLIKSLLEPHLHLDIHQTTPEKTAEELTELAIAKQA
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCC
DIIIACGGDGTVSAVAGALVNTNIPLGIIPRGTANAFCMALGIPSIAPIRGACDLILAGK
CEEEEECCCCCHHHHHHHHHCCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCEEEEECC
TRVIDVGRCNERTMILLAGVGYEAETIERADREAKNRWGVLAYMMAGWRQLTEQQLFDIE
EEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
IEIDGVAKTFQAGAITIANAAPPTSFLAQGLGKVIMNDGLLEVMIITSKNTLQAVNAGVR
EEECCHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHH
MFGSAIFQTPTQHEDTIALRTKRVKVSADPPQKVVLDGEIIGTTPIEVECIPNGLTVLAP
HHHHHHHCCCCCCCCEEEEEEEEEEEECCCCCEEEECCEEECCCCEEEEEECCCCEEEEC
PTNEAEEKPAWGFFS
CCCCCCCCCCCCCCC
>Mature Secondary Structure 
GNTIALLAYDSKKDNLVEFVKKHKIVLSRYQLVATESTAQYILQEVDIEINSVLAGPLG
CCEEEEEEECCCCHHHHHHHHHHHHHHHHEEEEEECHHHHHHHHHHCEEEEEEEECCCC
GDAQLAVEVTTGNVVAVICLVDYLFSQSYEPDIKLLLRLCQVHNVPIAINIATAEIIIKG
CCCEEEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEHHHHHHHH
FIQTTVAHLIFNPISGKGNAQQDLLLIKSLLEPHLHLDIHQTTPEKTAEELTELAIAKQA
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCC
DIIIACGGDGTVSAVAGALVNTNIPLGIIPRGTANAFCMALGIPSIAPIRGACDLILAGK
CEEEEECCCCCHHHHHHHHHCCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCEEEEECC
TRVIDVGRCNERTMILLAGVGYEAETIERADREAKNRWGVLAYMMAGWRQLTEQQLFDIE
EEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
IEIDGVAKTFQAGAITIANAAPPTSFLAQGLGKVIMNDGLLEVMIITSKNTLQAVNAGVR
EEECCHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHH
MFGSAIFQTPTQHEDTIALRTKRVKVSADPPQKVVLDGEIIGTTPIEVECIPNGLTVLAP
HHHHHHHCCCCCCCCEEEEEEEEEEEECCCCCEEEECCEEECCCCEEEEEECCCCEEEEC
PTNEAEEKPAWGFFS
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 13200 [C]

Specific activity: NA

Km value (mM): 0.5 {dihydroxyacetone} 0.47 {dihydroxyacetone} 0.2 {dihydroxyacetone} [C]

Substrates: Dihydroxyacetone phosphate [C]

Specific reaction: Dihydroxyacetone phosphate --> Methylglyoxal + Phosphate [C]

General reaction: elimination (C-O bond cleavage [C]

Inhibitor: 2-phosphoglycerate; 3-phosphoglycerate; arsenate; diphosphate; phosphate; phosphoenolpyruvate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]