Click here to switch to the map view.

The map label for this gene is kshA [H]

Identifier: 113474433

GI number: 113474433

Start: 926399

End: 927370

Strand: Reverse

Name: kshA [H]

Synonym: Tery_0576

Alternate gene names: 113474433

Gene position: 927370-926399 (Counterclockwise)

Preceding gene: 113474437

Following gene: 113474431

Centisome position: 11.97

GC content: 36.42

Gene sequence:

>972_bases
ATGTATTCAAATTTGCCTAATAGCTGGTTTCGTGTTGCTAGAAGTGAAGATTTATCTACCAAAGGAGTTATACCACTTCA
TTATTTTGGAAAAGACTTTGTACTGTTTCGTACAGAAGATGGCAAACCCCATATTTTTGATGCCCATTGTCCTCACTTAG
GGGCTCATCTTGCTCATGGGGGTAGAATAGAAGGCCAGACTCTAAGATGTCCCTACCACGGTTGGTTTTGGGATACAAAT
GGACAATGTGCTGCGACTCCTTATCCGAATAAAGCAAAACCTCAAGTCAAAATACAAAATTGGCCTGTACTTGAGCTGAA
TGGTCTAATAATGATGTATCATCACCACCAAGGAAAATTACCAGACTGGGAAATACCGCAAATACCAGAACTAATGTCTG
AGGAATGGACTCCTCTACGTTTAGTCCGTCAGTGGAAAGTACGCACTACTCTTCAAGATTATATGGACAATAGTGTAGAT
GTCTCTCACTTATATAATTTGCACAGCCGTACTTTTAAAAGCGCTCATAGTTATGGTGTAGAAATTGACGGACCAATGTT
AACTCATAGAATGTCCCAGAAATATAATTTATCTTCTTTAGCAGCGGGAAAATTAGTATTGGAAGATGGCTCAGTTACTA
CTATTTATTATGGACCTGCTTATGATGTGAGTTTTTACTGGACAGAAGGAAAGTTAAAGCTAGGTTTGCTAACTATTTTT
ACAGGAACACCAATAAATAATGAGTATCTTGATATTGCTATATTTTATAGTGTGAAAAAAGTTCTACCTTTTCCTTTAAG
CTTGATTTTAAATAAGATGTTAAAACAGGATGTTTTAGAAACTTTTGAACAAGATGTGAGTATTTTAGAGAATAAAAAAA
ATATTCGTAATCCACTTTTATATCAAAATGATGGCCCTGTGCGTCAGTCTCGAAGTTGGGCAAGTCAATTCTACTCCGAA
AATTCTGCTTGA

Upstream 100 bases:

>100_bases
TAGTTTGCATAAAATATTTTTTAATTTTTAGCTTTGGTAAGCGATCGCTATCCAAATAAACTAATAGCTGATTTTTGTCT
ATATTCAAAAAAAAATAATT

Downstream 100 bases:

>100_bases
TCAAATTAGTATAAATTGAATCCCGGAAATTGATGCTATATAGTCATGATCTATGTAGAAACCGGGTTGAGGAAACTCCC
TGGTTTGAACAATAATGGTC

Product: Rieske (2Fe-2S) protein

Products: NAD; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol [C]

Alternate protein names: Rieske-type oxygenase; RO [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MYSNLPNSWFRVARSEDLSTKGVIPLHYFGKDFVLFRTEDGKPHIFDAHCPHLGAHLAHGGRIEGQTLRCPYHGWFWDTN
GQCAATPYPNKAKPQVKIQNWPVLELNGLIMMYHHHQGKLPDWEIPQIPELMSEEWTPLRLVRQWKVRTTLQDYMDNSVD
VSHLYNLHSRTFKSAHSYGVEIDGPMLTHRMSQKYNLSSLAAGKLVLEDGSVTTIYYGPAYDVSFYWTEGKLKLGLLTIF
TGTPINNEYLDIAIFYSVKKVLPFPLSLILNKMLKQDVLETFEQDVSILENKKNIRNPLLYQNDGPVRQSRSWASQFYSE
NSA

Sequences:

>Translated_323_residues
MYSNLPNSWFRVARSEDLSTKGVIPLHYFGKDFVLFRTEDGKPHIFDAHCPHLGAHLAHGGRIEGQTLRCPYHGWFWDTN
GQCAATPYPNKAKPQVKIQNWPVLELNGLIMMYHHHQGKLPDWEIPQIPELMSEEWTPLRLVRQWKVRTTLQDYMDNSVD
VSHLYNLHSRTFKSAHSYGVEIDGPMLTHRMSQKYNLSSLAAGKLVLEDGSVTTIYYGPAYDVSFYWTEGKLKLGLLTIF
TGTPINNEYLDIAIFYSVKKVLPFPLSLILNKMLKQDVLETFEQDVSILENKKNIRNPLLYQNDGPVRQSRSWASQFYSE
NSA
>Mature_323_residues
MYSNLPNSWFRVARSEDLSTKGVIPLHYFGKDFVLFRTEDGKPHIFDAHCPHLGAHLAHGGRIEGQTLRCPYHGWFWDTN
GQCAATPYPNKAKPQVKIQNWPVLELNGLIMMYHHHQGKLPDWEIPQIPELMSEEWTPLRLVRQWKVRTTLQDYMDNSVD
VSHLYNLHSRTFKSAHSYGVEIDGPMLTHRMSQKYNLSSLAAGKLVLEDGSVTTIYYGPAYDVSFYWTEGKLKLGLLTIF
TGTPINNEYLDIAIFYSVKKVLPFPLSLILNKMLKQDVLETFEQDVSILENKKNIRNPLLYQNDGPVRQSRSWASQFYSE
NSA

Specific function: Catalyzes the opening of ring B of 1,4-androstadiene- 3,17,-dione (ADD) and 4-androstene-3,17-dione (ADD) with concomitant aromatization of ring A. The ring B is subsequently hydroxylated to yield a catechol and then subject to meta- cleavage [H]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI71983709, Length=349, Percent_Identity=28.9398280802292, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI161085896, Length=338, Percent_Identity=27.5147928994083, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017941 [H]

Pfam domain/function: PF00355 Rieske [H]

EC number: 1.14.1.- [C]

Molecular weight: Translated: 37236; Mature: 37236

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSNLPNSWFRVARSEDLSTKGVIPLHYFGKDFVLFRTEDGKPHIFDAHCPHLGAHLAHG
CCCCCCCHHHHHHCCCCCCCCCEEEEEECCCEEEEEEECCCCCCEEECCCCCHHHHHCCC
GRIEGQTLRCPYHGWFWDTNGQCAATPYPNKAKPQVKIQNWPVLELNGLIMMYHHHQGKL
CEECCCEEECCCCCEEECCCCCEEECCCCCCCCCCEEECCCCEEEECCEEEEEEECCCCC
PDWEIPQIPELMSEEWTPLRLVRQWKVRTTLQDYMDNSVDVSHLYNLHSRTFKSAHSYGV
CCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC
EIDGPMLTHRMSQKYNLSSLAAGKLVLEDGSVTTIYYGPAYDVSFYWTEGKLKLGLLTIF
EECCHHHHHHHHCCCCHHHHCCCEEEEECCCEEEEEECCCEEEEEEEECCEEEEEEEEEE
TGTPINNEYLDIAIFYSVKKVLPFPLSLILNKMLKQDVLETFEQDVSILENKKNIRNPLL
ECCCCCCCEEEEEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
YQNDGPVRQSRSWASQFYSENSA
ECCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MYSNLPNSWFRVARSEDLSTKGVIPLHYFGKDFVLFRTEDGKPHIFDAHCPHLGAHLAHG
CCCCCCCHHHHHHCCCCCCCCCEEEEEECCCEEEEEEECCCCCCEEECCCCCHHHHHCCC
GRIEGQTLRCPYHGWFWDTNGQCAATPYPNKAKPQVKIQNWPVLELNGLIMMYHHHQGKL
CEECCCEEECCCCCEEECCCCCEEECCCCCCCCCCEEECCCCEEEECCEEEEEEECCCCC
PDWEIPQIPELMSEEWTPLRLVRQWKVRTTLQDYMDNSVDVSHLYNLHSRTFKSAHSYGV
CCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC
EIDGPMLTHRMSQKYNLSSLAAGKLVLEDGSVTTIYYGPAYDVSFYWTEGKLKLGLLTIF
EECCHHHHHHHHCCCCHHHHCCCEEEEECCCEEEEEECCCEEEEEEEECCEEEEEEEEEE
TGTPINNEYLDIAIFYSVKKVLPFPLSLILNKMLKQDVLETFEQDVSILENKKNIRNPLL
ECCCCCCCEEEEEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
YQNDGPVRQSRSWASQFYSENSA
ECCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2Fe-2S. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; Proton; O2; 3-phenylpropionate [C]

Specific reaction: 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD 3-phenylpropionate + NADH + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]