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The map label for this gene is tuf

Identifier: 113474344

GI number: 113474344

Start: 755363

End: 756592

Strand: Direct

Name: tuf

Synonym: Tery_0476

Alternate gene names: 113474344

Gene position: 755363-756592 (Clockwise)

Preceding gene: 113474343

Following gene: 113474345

Centisome position: 9.75

GC content: 41.22

Gene sequence:

>1230_bases
ATGGCACGTGCAAAATTTGAAAGAAATAAACCTCATGTCAATATTGGAACTATTGGCCATGTAGACCACGGTAAAACAAC
CTTAACTGCTGCAATTACAATGAGTCTAGCAGCGCAAGGAAAGGCTAAGGCAAGAAATTATGCAGATATTGATGCAGCTC
CAGAAGAGAAAGCACGGGGTATCACAATTAATACTGCTCATGTAGAGTATGAAACAGAAGGACGTCACTATGCTCATGTA
GATTGTCCAGGACATGCTGACTACGTAAAAAATATGATTACAGGTGCAGCTCAAATGGATGGTGCAATTCTAGTAGTTTC
AGCTGCAGATGGCCCAATGCCCCAAACTCGTGAGCATATTCTGTTAGCAAAACAAGTGGGGGTTCCTAATATAGTTATCT
TCCTTAACAAGCAAGATATGGTAGATGATGAAGAACTTTTAGAACTAGTGGAACTGGAAGTTAGGGAACTTTTAAGCGAC
TATGATTTTGATGGAGATAATATTCCTATCGTGGCTGGTTCTGCTTTACAGGCAGTAGAAGCACTAACAGCAAACCCAGG
AATAGGAAAGGGAGAAAATGAATGGGTAGATAAAATTCTCTCTCTGATGGATGAAGTTGATGGGTACATTCCTCAACCAG
AACGTGATGTTGATAAGCCATTCTTAATGGCGGTAGAAGACGTATTTTCTATTACGGGTCGCGGTACTGTAGCTACAGGA
CGGATTGAACGTGGTAAGATAAAAGTTGGGGAAACTGTAGAATTGGTTGGAATTAAAGATACTCGCAATAGTACTGTTAC
AGGTGTGGAAATGTTCCAGAAAATTCTGGACGAAGGAATGGCTGGAGATAATGTAGGATTACTACTACGTGGTATGCAGA
AAGATGATATCCAACGTGGTATGGTTTTAGCTAAATCAGGTTCTATTACTCCCCATAAGAAGTTTGAGTCAGAAGTATAT
GTTCTCAAAAAGGAGGAAGGAGGCCGTCATACACCTTTCTTCCCAAATTATCGCCCTCAATTCTATATTCGGACAACAGA
TGTGACTGGTGCTATTGAGTCTTTTACTGCTGATGATGGTAGTGTGGCAGAGATGGTCATGCCTGGTGACAGGATTAAAA
TGACAGTTCAACTAATCAATCCAGTGGCCATTGAACAAGGTATGCGTTTTGCCATTCGTGAAGGTGGGAGAACTATTGGC
GCTGGTGTTGTCTCTAAGATTGTAGAGTAG

Upstream 100 bases:

>100_bases
AAGCATGATTTTCAGAGTTTATCTTATTATTAATTAGTGTATCAATTTCCTAATTTAAGGGAAGCAGAATTAACTACGAA
AAAAAAGGAAAAAGTTATTA

Downstream 100 bases:

>100_bases
GAAAACGTCCTGAGAAATTAAGTAGTAGAAAAGCAGTTATGTAAGTTATTGATTTTTGCTTTTCTGCTGCTTATTTAACG
AAAGTCTAAAACTTCGCCTT

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MARAKFERNKPHVNIGTIGHVDHGKTTLTAAITMSLAAQGKAKARNYADIDAAPEEKARGITINTAHVEYETEGRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLAKQVGVPNIVIFLNKQDMVDDEELLELVELEVRELLSD
YDFDGDNIPIVAGSALQAVEALTANPGIGKGENEWVDKILSLMDEVDGYIPQPERDVDKPFLMAVEDVFSITGRGTVATG
RIERGKIKVGETVELVGIKDTRNSTVTGVEMFQKILDEGMAGDNVGLLLRGMQKDDIQRGMVLAKSGSITPHKKFESEVY
VLKKEEGGRHTPFFPNYRPQFYIRTTDVTGAIESFTADDGSVAEMVMPGDRIKMTVQLINPVAIEQGMRFAIREGGRTIG
AGVVSKIVE

Sequences:

>Translated_409_residues
MARAKFERNKPHVNIGTIGHVDHGKTTLTAAITMSLAAQGKAKARNYADIDAAPEEKARGITINTAHVEYETEGRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLAKQVGVPNIVIFLNKQDMVDDEELLELVELEVRELLSD
YDFDGDNIPIVAGSALQAVEALTANPGIGKGENEWVDKILSLMDEVDGYIPQPERDVDKPFLMAVEDVFSITGRGTVATG
RIERGKIKVGETVELVGIKDTRNSTVTGVEMFQKILDEGMAGDNVGLLLRGMQKDDIQRGMVLAKSGSITPHKKFESEVY
VLKKEEGGRHTPFFPNYRPQFYIRTTDVTGAIESFTADDGSVAEMVMPGDRIKMTVQLINPVAIEQGMRFAIREGGRTIG
AGVVSKIVE
>Mature_408_residues
ARAKFERNKPHVNIGTIGHVDHGKTTLTAAITMSLAAQGKAKARNYADIDAAPEEKARGITINTAHVEYETEGRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLAKQVGVPNIVIFLNKQDMVDDEELLELVELEVRELLSDY
DFDGDNIPIVAGSALQAVEALTANPGIGKGENEWVDKILSLMDEVDGYIPQPERDVDKPFLMAVEDVFSITGRGTVATGR
IERGKIKVGETVELVGIKDTRNSTVTGVEMFQKILDEGMAGDNVGLLLRGMQKDDIQRGMVLAKSGSITPHKKFESEVYV
LKKEEGGRHTPFFPNYRPQFYIRTTDVTGAIESFTADDGSVAEMVMPGDRIKMTVQLINPVAIEQGMRFAIREGGRTIGA
GVVSKIVE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=407, Percent_Identity=53.3169533169533, Blast_Score=452, Evalue=1e-127,
Organism=Homo sapiens, GI4503475, Length=450, Percent_Identity=28.8888888888889, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI223555963, Length=450, Percent_Identity=29.1111111111111, Blast_Score=165, Evalue=6e-41,
Organism=Homo sapiens, GI5729864, Length=450, Percent_Identity=29.1111111111111, Blast_Score=164, Evalue=9e-41,
Organism=Homo sapiens, GI4503471, Length=444, Percent_Identity=28.1531531531532, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI54607086, Length=330, Percent_Identity=27.8787878787879, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI46094014, Length=441, Percent_Identity=26.530612244898, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI194018522, Length=441, Percent_Identity=26.984126984127, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI194097354, Length=443, Percent_Identity=26.410835214447, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI194018520, Length=443, Percent_Identity=26.410835214447, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI4503507, Length=277, Percent_Identity=27.0758122743682, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI94966754, Length=142, Percent_Identity=32.3943661971831, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI157426893, Length=353, Percent_Identity=22.6628895184136, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1790412, Length=408, Percent_Identity=70.8333333333333, Blast_Score=561, Evalue=1e-161,
Organism=Escherichia coli, GI1789737, Length=408, Percent_Identity=70.8333333333333, Blast_Score=560, Evalue=1e-161,
Organism=Escherichia coli, GI2367247, Length=356, Percent_Identity=30.3370786516854, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI48994988, Length=291, Percent_Identity=31.2714776632302, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1789108, Length=334, Percent_Identity=27.8443113772455, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790835, Length=149, Percent_Identity=30.2013422818792, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=414, Percent_Identity=50, Blast_Score=387, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI25141371, Length=400, Percent_Identity=42.5, Blast_Score=322, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI17552884, Length=443, Percent_Identity=27.765237020316, Blast_Score=158, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI17569207, Length=443, Percent_Identity=27.765237020316, Blast_Score=158, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI115532067, Length=446, Percent_Identity=26.6816143497758, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32566629, Length=452, Percent_Identity=27.6548672566372, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI115532065, Length=442, Percent_Identity=26.6968325791855, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI32566303, Length=439, Percent_Identity=27.1070615034169, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17506081, Length=282, Percent_Identity=29.7872340425532, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=38.0952380952381, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17565438, Length=429, Percent_Identity=23.3100233100233, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17557151, Length=282, Percent_Identity=25.177304964539, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=407, Percent_Identity=62.4078624078624, Blast_Score=521, Evalue=1e-149,
Organism=Saccharomyces cerevisiae, GI6325337, Length=443, Percent_Identity=29.5711060948081, Blast_Score=162, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6319594, Length=443, Percent_Identity=29.5711060948081, Blast_Score=162, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6322937, Length=456, Percent_Identity=24.780701754386, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6320377, Length=328, Percent_Identity=29.2682926829268, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320863, Length=277, Percent_Identity=26.3537906137184, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323320, Length=283, Percent_Identity=28.6219081272085, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=222, Percent_Identity=29.7297297297297, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI281363316, Length=403, Percent_Identity=57.3200992555831, Blast_Score=470, Evalue=1e-133,
Organism=Drosophila melanogaster, GI17864358, Length=403, Percent_Identity=57.3200992555831, Blast_Score=470, Evalue=1e-133,
Organism=Drosophila melanogaster, GI19921738, Length=399, Percent_Identity=46.8671679197995, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI45553807, Length=442, Percent_Identity=28.0542986425339, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI45553816, Length=442, Percent_Identity=28.0542986425339, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24651721, Length=442, Percent_Identity=28.0542986425339, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI17864154, Length=442, Percent_Identity=28.0542986425339, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24652838, Length=445, Percent_Identity=27.6404494382022, Blast_Score=151, Evalue=8e-37,
Organism=Drosophila melanogaster, GI17137572, Length=445, Percent_Identity=27.6404494382022, Blast_Score=151, Evalue=8e-37,
Organism=Drosophila melanogaster, GI19922690, Length=313, Percent_Identity=29.3929712460064, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI45550900, Length=455, Percent_Identity=26.5934065934066, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI17137380, Length=453, Percent_Identity=27.1523178807947, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24647046, Length=302, Percent_Identity=27.8145695364238, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24647048, Length=367, Percent_Identity=26.4305177111717, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI28572034, Length=164, Percent_Identity=33.5365853658537, Blast_Score=66, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24582462, Length=146, Percent_Identity=33.5616438356164, Blast_Score=65, Evalue=8e-11,
Organism=Drosophila melanogaster, GI78706572, Length=255, Percent_Identity=26.2745098039216, Blast_Score=65, Evalue=1e-10,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_TRIEI (Q118Z2)

Other databases:

- EMBL:   CP000393
- RefSeq:   YP_720405.1
- ProteinModelPortal:   Q118Z2
- SMR:   Q118Z2
- STRING:   Q118Z2
- GeneID:   4241688
- GenomeReviews:   CP000393_GR
- KEGG:   ter:Tery_0476
- NMPDR:   fig|203124.1.peg.4541
- eggNOG:   COG0050
- HOGENOM:   HBG307581
- OMA:   FEGQVYV
- PhylomeDB:   Q118Z2
- ProtClustDB:   PRK00049
- BioCyc:   TERY203124:TERY_0476-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 44587; Mature: 44456

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARAKFERNKPHVNIGTIGHVDHGKTTLTAAITMSLAAQGKAKARNYADIDAAPEEKARG
CCCCCCCCCCCEEEECEEECCCCCCEEEEHHHHHHHHHCCCHHCCCCCCCCCCCCHHCCC
ITINTAHVEYETEGRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHE
LLAKQVGVPNIVIFLNKQDMVDDEELLELVELEVRELLSDYDFDGDNIPIVAGSALQAVE
EEEHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECHHHHHHH
ALTANPGIGKGENEWVDKILSLMDEVDGYIPQPERDVDKPFLMAVEDVFSITGRGTVATG
HHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEC
RIERGKIKVGETVELVGIKDTRNSTVTGVEMFQKILDEGMAGDNVGLLLRGMQKDDIQRG
CEECCEEEECCEEEEEEEECCCCCEEHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHC
MVLAKSGSITPHKKFESEVYVLKKEEGGRHTPFFPNYRPQFYIRTTDVTGAIESFTADDG
EEEEECCCCCCCHHCCCEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCHHHHCCCCCC
SVAEMVMPGDRIKMTVQLINPVAIEQGMRFAIREGGRTIGAGVVSKIVE
CEEEEECCCCCEEEEEEECCHHHHHHCCEEEHHCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
ARAKFERNKPHVNIGTIGHVDHGKTTLTAAITMSLAAQGKAKARNYADIDAAPEEKARG
CCCCCCCCCCEEEECEEECCCCCCEEEEHHHHHHHHHCCCHHCCCCCCCCCCCCHHCCC
ITINTAHVEYETEGRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHE
LLAKQVGVPNIVIFLNKQDMVDDEELLELVELEVRELLSDYDFDGDNIPIVAGSALQAVE
EEEHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECHHHHHHH
ALTANPGIGKGENEWVDKILSLMDEVDGYIPQPERDVDKPFLMAVEDVFSITGRGTVATG
HHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEC
RIERGKIKVGETVELVGIKDTRNSTVTGVEMFQKILDEGMAGDNVGLLLRGMQKDDIQRG
CEECCEEEECCEEEEEEEECCCCCEEHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHC
MVLAKSGSITPHKKFESEVYVLKKEEGGRHTPFFPNYRPQFYIRTTDVTGAIESFTADDG
EEEEECCCCCCCHHCCCEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCHHHHCCCCCC
SVAEMVMPGDRIKMTVQLINPVAIEQGMRFAIREGGRTIGAGVVSKIVE
CEEEEECCCCCEEEEEEECCHHHHHHCCEEEHHCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA