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The map label for this gene is kefA [H]

Identifier: 113474110

GI number: 113474110

Start: 315196

End: 317253

Strand: Direct

Name: kefA [H]

Synonym: Tery_0209

Alternate gene names: 113474110

Gene position: 315196-317253 (Clockwise)

Preceding gene: 113474108

Following gene: 113474113

Centisome position: 4.07

GC content: 30.17

Gene sequence:

>2058_bases
ATGTGGCATAAATATTTGTGGCTAAAGTTAAAAAAAATAGCAAAATTAGAACTACCAAATTATCTTAAAGCAACAAATAA
TCCCTTAACTTTTCCAAGCAAGAGAGAAACTAAAACCCACCAAAAGCAAATCGCCAAAATATTAATTATTGCTTGTTTAA
TACCTTTACTATTTTTTATAATACCAGCTTGGGGACAAGCCATTGATAATTATCTAGAAAAAGCTCCAGTAGAATTGAAT
GGTCAAAAATTATTTGAAGTAGCAGGTAATAAAGAAAAAAATTTGACTGCTATTGAACGAGCGGCAATAATTAGCTCAAA
ATTAGAAAATATCTTCAGCTCAGATGAATCTATAGAGGTAAATATAGATAAGCAAAACAATCAAGTAATTTTGCTTACTA
ATAATAACTATTTACTAACTATAACTGCTGCAGATGTTAAAGGAAGAATGAAAACAGAAGAGCAAGCAATCATCTGGAAA
GATAAGATTGAAGAAAGTTTTAAAGCAGGCAAAAAAAACTCTCATTCTCCTTACTTATTAAAAGAATTATCCATTAGCAT
TGTTTTAATTTTAGCAGCTTTAAGTATAGAGCAATTTTGGGGTTTATTAAAGTTTAAAATTAGCAATTTCTGTGAAAAAA
AATATCCTCAAATTGTTAGTTATTTACCAAAATTTTTGCCATTAACGATAATTTCTATCCGAGTCATAGTATGGATAAAA
ATCACCCTATATATCACCTATCTACTACCATTTACTCGTGAGCCTAGTGAGAAACTATTAAGTATTTTATATAATAGTTT
TACTTCTCGTATCTTTGACTTAGGCAACAAAGGTCTTTCCATTGTTGATTTGGTATTTTTAACTGGGTTAACTATTGGAA
TGTGGAAAGGAGTTGGGTACTTTATTGATATTTTTAGAACTAAAATTGTAAGTTTAACAGGAGCAGAGCGAAGTGTTCAA
GATACAATCAGTTTCTTAGCTAAATATGGATTAATTTTTTTTGGTATACTAATTATTCTACAACTTTGGGGTGTAGATAT
TAGTTCTTTGGCAATTATTGCTAGTGTTCTTGGTGTAGGTATTGGTTTTGGTCTACAGAATATTGCTAATAATTTTATGA
GTGGTATTATTCTAGTTTTTGAGCGACCAATTCAAGTGGGTGACTTTATTGAAGTAGGAAATTTAGTAGGCATTGTAAAA
AAAATAGGTTTGCGTAGCACTTACATTACAACTTTAGACAAAGTTTCTTTAATTGTTCCTAACTCTAGATTTCTAGAAAA
TGAAGTTTTAAATTGGAGTCATGGTAACCCTATTTCTAGGATAAAAATACCGATAGGTGTTGCTTATGGTTCTAATGTTA
AGTTAGTTAAGAAAGCTATATTAGAAGTAGCAAAAAGTCATCCAGAAGTATTATTACATCCCTCCCCACAAATTTGGTTT
CAAGAATTTGGTGATAGTTCTCTAAAATTTCAATTGTTGATTTGGACTAGAGAACCACAAAAACAATATCAACTAAAAAG
TGAGTTAAATTATCGCATTGAGGCAAGTCTACAAAGATATAAAATTGAAATTCCTTTTCCTCAAAGAGATTTGCATTTAA
AATCTCCCAAAATAGAGCAAATGATAGAGATTTGGATGCGGAAAAATTCACCTCCTCAAGAACAAATTTACTATCCGAAT
AGCCTTAAGTTTAAGTTTGAAAATAATGCTTCTCAGTTAGATATAGATGAGGAGGATGAAGCCAAAGCATTGACTAAAGA
TTCTCTCAATAATCAAACCAACAAAAATATAAATATAGATACCTTAGTTGAAGAAATGCGTGGTCATAATGGAGTATCAA
TTAAAGATCGTCAACATAGGTGGGATGTCTACTCAAAATGTTTTGTTGGTTCTGAGGCAGTACAGTGGTTAATGAGAACT
CAAAAACTTAACTTAAGTCAAGCAATTTCTATGGGTCAATTATTAATAGACCGTGGCTTAATTCATCATGTTGTAGATAA
ACATAGTTTTAAAAATCAGTATATTTTCTATCGTTTTTATGAAGATGAAAACTACTAG

Upstream 100 bases:

>100_bases
AAAAAAGATCGTGCTAAAAATTCAGCAAATTTTAGTACATCAGTTTTTCCACATCAATAGTTTATAGTGAAATATTTATT
AGTAGTTTTTGAGAGTGACT

Downstream 100 bases:

>100_bases
GGTGAATATGTTTTTAGTAAACTAAAAATTGTCCGTAATTATTAGTCACTTTAATATTTGAATAATTAGTTGTAGGGTTG
AAGGGATACTGGAGAAAAAT

Product: mechanosensitive ion channel MscS

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 685; Mature: 685

Protein sequence:

>685_residues
MWHKYLWLKLKKIAKLELPNYLKATNNPLTFPSKRETKTHQKQIAKILIIACLIPLLFFIIPAWGQAIDNYLEKAPVELN
GQKLFEVAGNKEKNLTAIERAAIISSKLENIFSSDESIEVNIDKQNNQVILLTNNNYLLTITAADVKGRMKTEEQAIIWK
DKIEESFKAGKKNSHSPYLLKELSISIVLILAALSIEQFWGLLKFKISNFCEKKYPQIVSYLPKFLPLTIISIRVIVWIK
ITLYITYLLPFTREPSEKLLSILYNSFTSRIFDLGNKGLSIVDLVFLTGLTIGMWKGVGYFIDIFRTKIVSLTGAERSVQ
DTISFLAKYGLIFFGILIILQLWGVDISSLAIIASVLGVGIGFGLQNIANNFMSGIILVFERPIQVGDFIEVGNLVGIVK
KIGLRSTYITTLDKVSLIVPNSRFLENEVLNWSHGNPISRIKIPIGVAYGSNVKLVKKAILEVAKSHPEVLLHPSPQIWF
QEFGDSSLKFQLLIWTREPQKQYQLKSELNYRIEASLQRYKIEIPFPQRDLHLKSPKIEQMIEIWMRKNSPPQEQIYYPN
SLKFKFENNASQLDIDEEDEAKALTKDSLNNQTNKNINIDTLVEEMRGHNGVSIKDRQHRWDVYSKCFVGSEAVQWLMRT
QKLNLSQAISMGQLLIDRGLIHHVVDKHSFKNQYIFYRFYEDENY

Sequences:

>Translated_685_residues
MWHKYLWLKLKKIAKLELPNYLKATNNPLTFPSKRETKTHQKQIAKILIIACLIPLLFFIIPAWGQAIDNYLEKAPVELN
GQKLFEVAGNKEKNLTAIERAAIISSKLENIFSSDESIEVNIDKQNNQVILLTNNNYLLTITAADVKGRMKTEEQAIIWK
DKIEESFKAGKKNSHSPYLLKELSISIVLILAALSIEQFWGLLKFKISNFCEKKYPQIVSYLPKFLPLTIISIRVIVWIK
ITLYITYLLPFTREPSEKLLSILYNSFTSRIFDLGNKGLSIVDLVFLTGLTIGMWKGVGYFIDIFRTKIVSLTGAERSVQ
DTISFLAKYGLIFFGILIILQLWGVDISSLAIIASVLGVGIGFGLQNIANNFMSGIILVFERPIQVGDFIEVGNLVGIVK
KIGLRSTYITTLDKVSLIVPNSRFLENEVLNWSHGNPISRIKIPIGVAYGSNVKLVKKAILEVAKSHPEVLLHPSPQIWF
QEFGDSSLKFQLLIWTREPQKQYQLKSELNYRIEASLQRYKIEIPFPQRDLHLKSPKIEQMIEIWMRKNSPPQEQIYYPN
SLKFKFENNASQLDIDEEDEAKALTKDSLNNQTNKNINIDTLVEEMRGHNGVSIKDRQHRWDVYSKCFVGSEAVQWLMRT
QKLNLSQAISMGQLLIDRGLIHHVVDKHSFKNQYIFYRFYEDENY
>Mature_685_residues
MWHKYLWLKLKKIAKLELPNYLKATNNPLTFPSKRETKTHQKQIAKILIIACLIPLLFFIIPAWGQAIDNYLEKAPVELN
GQKLFEVAGNKEKNLTAIERAAIISSKLENIFSSDESIEVNIDKQNNQVILLTNNNYLLTITAADVKGRMKTEEQAIIWK
DKIEESFKAGKKNSHSPYLLKELSISIVLILAALSIEQFWGLLKFKISNFCEKKYPQIVSYLPKFLPLTIISIRVIVWIK
ITLYITYLLPFTREPSEKLLSILYNSFTSRIFDLGNKGLSIVDLVFLTGLTIGMWKGVGYFIDIFRTKIVSLTGAERSVQ
DTISFLAKYGLIFFGILIILQLWGVDISSLAIIASVLGVGIGFGLQNIANNFMSGIILVFERPIQVGDFIEVGNLVGIVK
KIGLRSTYITTLDKVSLIVPNSRFLENEVLNWSHGNPISRIKIPIGVAYGSNVKLVKKAILEVAKSHPEVLLHPSPQIWF
QEFGDSSLKFQLLIWTREPQKQYQLKSELNYRIEASLQRYKIEIPFPQRDLHLKSPKIEQMIEIWMRKNSPPQEQIYYPN
SLKFKFENNASQLDIDEEDEAKALTKDSLNNQTNKNINIDTLVEEMRGHNGVSIKDRQHRWDVYSKCFVGSEAVQWLMRT
QKLNLSQAISMGQLLIDRGLIHHVVDKHSFKNQYIFYRFYEDENY

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI34452732, Length=74, Percent_Identity=43.2432432432432, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI47578115, Length=83, Percent_Identity=39.7590361445783, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI47578117, Length=83, Percent_Identity=39.7590361445783, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI189571664, Length=81, Percent_Identity=41.9753086419753, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI155030204, Length=73, Percent_Identity=38.3561643835616, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI155030206, Length=73, Percent_Identity=38.3561643835616, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI1786670, Length=268, Percent_Identity=32.4626865671642, Blast_Score=174, Evalue=2e-44,
Organism=Escherichia coli, GI2367355, Length=214, Percent_Identity=32.7102803738318, Blast_Score=137, Evalue=3e-33,
Organism=Escherichia coli, GI1789291, Length=222, Percent_Identity=27.9279279279279, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI1787591, Length=228, Percent_Identity=28.5087719298246, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6322598, Length=108, Percent_Identity=36.1111111111111, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 78696; Mature: 78696

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS50186 DEP ; PS01246 UPF0003

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWHKYLWLKLKKIAKLELPNYLKATNNPLTFPSKRETKTHQKQIAKILIIACLIPLLFFI
CCCHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IPAWGQAIDNYLEKAPVELNGQKLFEVAGNKEKNLTAIERAAIISSKLENIFSSDESIEV
HHHHHHHHHHHHHHCCCCCCCHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEE
NIDKQNNQVILLTNNNYLLTITAADVKGRMKTEEQAIIWKDKIEESFKAGKKNSHSPYLL
EEECCCCEEEEEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHH
KELSISIVLILAALSIEQFWGLLKFKISNFCEKKYPQIVSYLPKFLPLTIISIRVIVWIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITLYITYLLPFTREPSEKLLSILYNSFTSRIFDLGNKGLSIVDLVFLTGLTIGMWKGVGY
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
FIDIFRTKIVSLTGAERSVQDTISFLAKYGLIFFGILIILQLWGVDISSLAIIASVLGVG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
IGFGLQNIANNFMSGIILVFERPIQVGDFIEVGNLVGIVKKIGLRSTYITTLDKVSLIVP
HCCCHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHEEEEEC
NSRFLENEVLNWSHGNPISRIKIPIGVAYGSNVKLVKKAILEVAKSHPEVLLHPSPQIWF
CCHHHHHHCCCCCCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHCCCCEEECCCCHHHH
QEFGDSSLKFQLLIWTREPQKQYQLKSELNYRIEASLQRYKIEIPFPQRDLHLKSPKIEQ
HHCCCCCEEEEEEEEECCHHHHHHHHHHCCEEEEECEEEEEEECCCCCCCCCCCCCCHHH
MIEIWMRKNSPPQEQIYYPNSLKFKFENNASQLDIDEEDEAKALTKDSLNNQTNKNINID
HHHHHHHCCCCCHHHEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHH
TLVEEMRGHNGVSIKDRQHRWDVYSKCFVGSEAVQWLMRTQKLNLSQAISMGQLLIDRGL
HHHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
IHHVVDKHSFKNQYIFYRFYEDENY
HHHHHHHHCCCCCEEEEEEECCCCC
>Mature Secondary Structure
MWHKYLWLKLKKIAKLELPNYLKATNNPLTFPSKRETKTHQKQIAKILIIACLIPLLFFI
CCCHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IPAWGQAIDNYLEKAPVELNGQKLFEVAGNKEKNLTAIERAAIISSKLENIFSSDESIEV
HHHHHHHHHHHHHHCCCCCCCHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEE
NIDKQNNQVILLTNNNYLLTITAADVKGRMKTEEQAIIWKDKIEESFKAGKKNSHSPYLL
EEECCCCEEEEEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHH
KELSISIVLILAALSIEQFWGLLKFKISNFCEKKYPQIVSYLPKFLPLTIISIRVIVWIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITLYITYLLPFTREPSEKLLSILYNSFTSRIFDLGNKGLSIVDLVFLTGLTIGMWKGVGY
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
FIDIFRTKIVSLTGAERSVQDTISFLAKYGLIFFGILIILQLWGVDISSLAIIASVLGVG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
IGFGLQNIANNFMSGIILVFERPIQVGDFIEVGNLVGIVKKIGLRSTYITTLDKVSLIVP
HCCCHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHEEEEEC
NSRFLENEVLNWSHGNPISRIKIPIGVAYGSNVKLVKKAILEVAKSHPEVLLHPSPQIWF
CCHHHHHHCCCCCCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHCCCCEEECCCCHHHH
QEFGDSSLKFQLLIWTREPQKQYQLKSELNYRIEASLQRYKIEIPFPQRDLHLKSPKIEQ
HHCCCCCEEEEEEEEECCHHHHHHHHHHCCEEEEECEEEEEEECCCCCCCCCCCCCCHHH
MIEIWMRKNSPPQEQIYYPNSLKFKFENNASQLDIDEEDEAKALTKDSLNNQTNKNINID
HHHHHHHCCCCCHHHEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHH
TLVEEMRGHNGVSIKDRQHRWDVYSKCFVGSEAVQWLMRTQKLNLSQAISMGQLLIDRGL
HHHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
IHHVVDKHSFKNQYIFYRFYEDENY
HHHHHHHHCCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]