BacMap

Definition	Alcanivorax borkumensis SK2 chromosome, complete genome.
Accession	NC_008260
Length	3,120,143

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The map label for this gene is ephD [H]

Identifier: 110835255

GI number: 110835255

Start: 2717029

End: 2717877

Strand: Reverse

Name: ephD [H]

Synonym: ABO_2394

Alternate gene names: 110835255

Gene position: 2717877-2717029 (Counterclockwise)

Preceding gene: 110835257

Following gene: 110835254

Centisome position: 87.11

GC content: 57.13

Gene sequence:

>849_bases
ATGATGAATTTTTTGAAGCAGCGTTTTATCAAGCCCAGTAAAAATGCCTATGCGGTGGTGACCGGCGCCGGTAGTGGTAT
CGGTCGCAGCTTTGCACTGGAGCTGGGCAAGCGCGGTGGCGCGGTGGTCTGTGCCGATGTGAATCTGAAGGCTGCCGAAG
AAACGGTGGCGTTACTGAACGCGCAGGGCGCGAAGGGGTTTGCCGTTCACTGTGACGTGGGCGATGCCAAGCAGGTCGCT
GCCTTGGCCGAAAAATCCGCAGCGTTGCTGGGGCACCCGGTAACCCTGGTCGTCAACAACGCCGGCGTTGGTCTGGGGGG
CATGTTCGATGAGCTGAGCCTGGAAGACTGGCAGTGGTGCATGCATGTGAATCTGTGGGGCGTGATCAACGGGTGCCATT
ATTTTGTACCCGGTTTCAAGACGCAGGGGCACGGCGCCATTATCAATGTGGCGTCTGCGGCGTCGTACACAGCGGCGCCA
GAAATGAGCGCCTATAACGTGAGCAAGGCAGGGGTACGTGCCCTGTCTGAAACCCTGTTTTCGGAATTGGCCAAGGATAA
TATTCGGGTGAATGTGCTGTGCCCGACACTGGTGCCCACCAATATTATTAAGAATGGCCGTATCCCCGACCGGTACGCCA
AGCTGGCGGATCATGCGCTAATGAACTACGCCATGACCACCAGTGAGAGCGTGGCCAAGCTGACCCTGAACCGCCTGGAT
AAGGGCGAGCTGTACACCACCCCCCAGGTGGACGCCAAACTGTTTTGGATAATGAAGCGGGCGTCGCCCCGTCTTTACGC
CAAGTTCATGAGTTTCTCTTACCGCTTTGTTCGCACCAAGGAGGCATAA

Upstream 100 bases:

>100_bases
CGGAATCTGATTAAGGCATGGGTGCCGACACTGCGAGCGTGATGTTGCCGGTGCGCCTGTTTAATCGAGCGTGTCGCCAT
AACCTGTTGAGATAATCGTA

Downstream 100 bases:

>100_bases
CATGGCCGCAGCAAAAAAAACCAAACCCTCAGCGGCCAAGGGCCCTGCGGAAAAAATTTATGATTCGTTTATTGTCGGTG
CCGGTATCTCCGGTATTGCC

Product: short chain dehydrogenase/reductase family oxidoreductase

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MMNFLKQRFIKPSKNAYAVVTGAGSGIGRSFALELGKRGGAVVCADVNLKAAEETVALLNAQGAKGFAVHCDVGDAKQVA
ALAEKSAALLGHPVTLVVNNAGVGLGGMFDELSLEDWQWCMHVNLWGVINGCHYFVPGFKTQGHGAIINVASAASYTAAP
EMSAYNVSKAGVRALSETLFSELAKDNIRVNVLCPTLVPTNIIKNGRIPDRYAKLADHALMNYAMTTSESVAKLTLNRLD
KGELYTTPQVDAKLFWIMKRASPRLYAKFMSFSYRFVRTKEA

Sequences:

>Translated_282_residues
MMNFLKQRFIKPSKNAYAVVTGAGSGIGRSFALELGKRGGAVVCADVNLKAAEETVALLNAQGAKGFAVHCDVGDAKQVA
ALAEKSAALLGHPVTLVVNNAGVGLGGMFDELSLEDWQWCMHVNLWGVINGCHYFVPGFKTQGHGAIINVASAASYTAAP
EMSAYNVSKAGVRALSETLFSELAKDNIRVNVLCPTLVPTNIIKNGRIPDRYAKLADHALMNYAMTTSESVAKLTLNRLD
KGELYTTPQVDAKLFWIMKRASPRLYAKFMSFSYRFVRTKEA
>Mature_282_residues
MMNFLKQRFIKPSKNAYAVVTGAGSGIGRSFALELGKRGGAVVCADVNLKAAEETVALLNAQGAKGFAVHCDVGDAKQVA
ALAEKSAALLGHPVTLVVNNAGVGLGGMFDELSLEDWQWCMHVNLWGVINGCHYFVPGFKTQGHGAIINVASAASYTAAP
EMSAYNVSKAGVRALSETLFSELAKDNIRVNVLCPTLVPTNIIKNGRIPDRYAKLADHALMNYAMTTSESVAKLTLNRLD
KGELYTTPQVDAKLFWIMKRASPRLYAKFMSFSYRFVRTKEA

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI15277342, Length=268, Percent_Identity=30.2238805970149, Blast_Score=98, Evalue=9e-21,
Organism=Homo sapiens, GI25282469, Length=232, Percent_Identity=30.6034482758621, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI32483357, Length=192, Percent_Identity=32.2916666666667, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI142976729, Length=192, Percent_Identity=34.8958333333333, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI31542939, Length=211, Percent_Identity=30.3317535545024, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI40807363, Length=205, Percent_Identity=27.8048780487805, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI210032110, Length=205, Percent_Identity=30.2439024390244, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI126723191, Length=184, Percent_Identity=31.5217391304348, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI40254992, Length=186, Percent_Identity=31.1827956989247, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI310128830, Length=263, Percent_Identity=26.615969581749, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI310115468, Length=263, Percent_Identity=26.615969581749, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI33667109, Length=195, Percent_Identity=32.8205128205128, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI20149619, Length=207, Percent_Identity=28.5024154589372, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI5031737, Length=191, Percent_Identity=32.4607329842932, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI126723750, Length=193, Percent_Identity=30.5699481865285, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI4504505, Length=203, Percent_Identity=29.5566502463054, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI7705925, Length=248, Percent_Identity=31.4516129032258, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI50726952, Length=204, Percent_Identity=31.3725490196078, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI4503817, Length=185, Percent_Identity=29.1891891891892, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI4557649, Length=203, Percent_Identity=28.0788177339901, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI226371731, Length=194, Percent_Identity=29.3814432989691, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI224922801, Length=166, Percent_Identity=31.3253012048193, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI44680136, Length=189, Percent_Identity=28.042328042328, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI44680133, Length=189, Percent_Identity=28.042328042328, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI17738292, Length=189, Percent_Identity=28.042328042328, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787905, Length=201, Percent_Identity=30.8457711442786, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1789208, Length=203, Percent_Identity=31.5270935960591, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1786812, Length=200, Percent_Identity=35.5, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI2367175, Length=248, Percent_Identity=31.0483870967742, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI87082100, Length=184, Percent_Identity=31.5217391304348, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1790717, Length=186, Percent_Identity=28.494623655914, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787335, Length=187, Percent_Identity=29.4117647058824, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788459, Length=234, Percent_Identity=26.0683760683761, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082160, Length=214, Percent_Identity=27.1028037383178, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786701, Length=187, Percent_Identity=28.8770053475936, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787820, Length=218, Percent_Identity=27.9816513761468, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1789378, Length=220, Percent_Identity=25.9090909090909, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17567345, Length=188, Percent_Identity=32.4468085106383, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI193203115, Length=194, Percent_Identity=29.3814432989691, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI193203117, Length=186, Percent_Identity=30.1075268817204, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI25147288, Length=186, Percent_Identity=36.0215053763441, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17555706, Length=202, Percent_Identity=31.6831683168317, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17564282, Length=211, Percent_Identity=27.4881516587678, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17508895, Length=197, Percent_Identity=23.3502538071066, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17561402, Length=189, Percent_Identity=31.7460317460317, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI193210355, Length=261, Percent_Identity=27.9693486590038, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17560676, Length=186, Percent_Identity=28.494623655914, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17559104, Length=204, Percent_Identity=27.9411764705882, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI115534694, Length=208, Percent_Identity=29.8076923076923, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17568967, Length=203, Percent_Identity=28.0788177339901, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17562910, Length=196, Percent_Identity=28.0612244897959, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI71994600, Length=244, Percent_Identity=27.0491803278689, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI71994604, Length=212, Percent_Identity=25, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323882, Length=188, Percent_Identity=30.8510638297872, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6322861, Length=209, Percent_Identity=29.1866028708134, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28571526, Length=215, Percent_Identity=33.0232558139535, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI21357041, Length=218, Percent_Identity=32.1100917431193, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24649181, Length=189, Percent_Identity=33.8624338624339, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24639444, Length=196, Percent_Identity=33.1632653061224, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI23397609, Length=214, Percent_Identity=32.2429906542056, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281364892, Length=198, Percent_Identity=29.2929292929293, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24640492, Length=193, Percent_Identity=26.9430051813472, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI161076933, Length=192, Percent_Identity=30.7291666666667, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24584748, Length=195, Percent_Identity=30.7692307692308, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21355631, Length=167, Percent_Identity=31.7365269461078, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356301, Length=184, Percent_Identity=29.8913043478261, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI18859837, Length=202, Percent_Identity=30.6930693069307, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI28574402, Length=196, Percent_Identity=30.1020408163265, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24584744, Length=196, Percent_Identity=30.1020408163265, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24584752, Length=202, Percent_Identity=30.6930693069307, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24644339, Length=213, Percent_Identity=28.6384976525822, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24643142, Length=216, Percent_Identity=29.6296296296296, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24640442, Length=195, Percent_Identity=28.7179487179487, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17737361, Length=203, Percent_Identity=30.0492610837438, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24581726, Length=213, Percent_Identity=27.6995305164319, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI21355319, Length=189, Percent_Identity=28.5714285714286, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 30483; Mature: 30483

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNFLKQRFIKPSKNAYAVVTGAGSGIGRSFALELGKRGGAVVCADVNLKAAEETVALLN
CCHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHC
AQGAKGFAVHCDVGDAKQVAALAEKSAALLGHPVTLVVNNAGVGLGGMFDELSLEDWQWC
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHCCCCCCEEEE
MHVNLWGVINGCHYFVPGFKTQGHGAIINVASAASYTAAPEMSAYNVSKAGVRALSETLF
EEEEEEEEECCEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHH
SELAKDNIRVNVLCPTLVPTNIIKNGRIPDRYAKLADHALMNYAMTTSESVAKLTLNRLD
HHHHHCCEEEEEEECCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
KGELYTTPQVDAKLFWIMKRASPRLYAKFMSFSYRFVRTKEA
CCCEEECCCCCCEEEEEEHHCCHHHHHHHHHHHEEEEEECCC
>Mature Secondary Structure
MMNFLKQRFIKPSKNAYAVVTGAGSGIGRSFALELGKRGGAVVCADVNLKAAEETVALLN
CCHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHC
AQGAKGFAVHCDVGDAKQVAALAEKSAALLGHPVTLVVNNAGVGLGGMFDELSLEDWQWC
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHCCCCCCEEEE
MHVNLWGVINGCHYFVPGFKTQGHGAIINVASAASYTAAPEMSAYNVSKAGVRALSETLF
EEEEEEEEECCEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHH
SELAKDNIRVNVLCPTLVPTNIIKNGRIPDRYAKLADHALMNYAMTTSESVAKLTLNRLD
HHHHHCCEEEEEEECCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
KGELYTTPQVDAKLFWIMKRASPRLYAKFMSFSYRFVRTKEA
CCCEEECCCCCCEEEEEEHHCCHHHHHHHHHHHEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]